Abstract:
:Peptide amidination labeling using S-methyl thioacetimidate (SMTA) is investigated in an attempt to increase the number and types of peptides that can be detected in a bottom-up proteomics experiment. This derivatization method affects the basicity of lysine residues and is shown here to significantly impact the idiosyncracies of peptide fragmentation and peptide detectability. The unique and highly reproducible fragmentation properties of SMTA-labeled peptides, such as the strong propensity for forming b1 fragment ions, can be further exploited to modify the scoring of peptide-spectrum pairs and improve peptide identification. To this end, we have developed a supervised postprocessing algorithm to exploit these characteristics of peptides labeled by SMTA. Our experiments show that although the overall number of identifications are similar, the SMTA modification enabled the detection of 16-26% peptides not previously observed in comparable CID/HCD tandem mass spectrometry experiments without SMTA labeling.
journal_name
J Proteome Resjournal_title
Journal of proteome researchauthors
Li S,Dabir A,Misal SA,Tang H,Radivojac P,Reilly JPdoi
10.1021/acs.jproteome.6b00468subject
Has Abstractpub_date
2016-10-07 00:00:00pages
3656-3665issue
10eissn
1535-3893issn
1535-3907journal_volume
15pub_type
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