Abstract:
:Glycosylation has significant effects on protein function and cell metastasis, which are important in cancer progression. It is of great interest to identify site-specific glycosylation in search of potential cancer biomarkers. However, the abundance of glycopeptides is low compared to that of nonglycopeptides after trypsin digestion of serum samples, and the mass spectrometric signals of glycopeptides are often masked by coeluting nonglycopeptides due to low ionization efficiency. Selective enrichment of glycopeptides from complex serum samples is essential for mass spectrometry (MS)-based analysis. Herein, a strategy has been optimized using LCA enrichment to improve the identification of core-fucosylation (CF) sites in serum of pancreatic cancer patients. The optimized strategy was then applied to analyze CF glycopeptide sites in 13 sets of serum samples from pancreatic cancer, chronic pancreatitis, healthy controls, and a standard reference. In total, 630 core-fucosylation sites were identified from 322 CF proteins in pancreatic cancer patient serum using an Orbitrap Elite mass spectrometer. Further data analysis revealed that 8 CF peptides exhibited a significant difference between pancreatic cancer and other controls, which may be potential diagnostic biomarkers for pancreatic cancer.
journal_name
J Proteome Resjournal_title
Journal of proteome researchauthors
Tan Z,Yin H,Nie S,Lin Z,Zhu J,Ruffin MT,Anderson MA,Simeone DM,Lubman DMdoi
10.1021/acs.jproteome.5b00068subject
Has Abstractpub_date
2015-04-03 00:00:00pages
1968-78issue
4eissn
1535-3893issn
1535-3907journal_volume
14pub_type
杂志文章abstract::The search for novel enzymes is an important but difficult task in functional genomics. Here, we present a systematic method based on in vitro assays in combination with metabolite profiling to discover novel enzymatic activities. A complex mixture of metabolites is incubated with purified candidate proteins and the r...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr0600576
更新日期:2006-08-01 00:00:00
abstract::Large-scale phosphoproteomic analysis employing liquid chromatography-tandem mass spectrometry (LC-MS/MS) often requires a significant amount of manual manipulation of phosphopeptide datasets in the post-acquisition phase. To assist in this process, we have created software, PhosphoPIC (PhosphoPeptide Identification a...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr0701153
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abstract::Colorectal cancer (CRC) is the third most common cancer worldwide and has poor prognosis. To identify the proteins involved in colorectal carcinogenesis, we employed 2-DE and MALDI-TOF/TOF-based proteomics approach to study the differentially expressed proteins in tumor and adjacent nontumor tissue samples. Samples fr...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr900365e
更新日期:2009-10-01 00:00:00
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journal_title:Journal of proteome research
pub_type: 杂志文章
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更新日期:2015-02-06 00:00:00
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journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr400376e
更新日期:2013-08-02 00:00:00
abstract::Transferrin purification from mice serum samples by immunoaffinity chromatography (IAC) was optimized in order to study the possible modifications occurring in its glycans in collagen-induced arthritis (CIA) samples. SDS-PAGE and nanoLC-MS/MS were used to monitor the IAC purification performance. Afterward, a relative...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/acs.jproteome.0c00016
更新日期:2020-04-03 00:00:00
abstract::Metabolically engineered Escherichia coli has previously been used to degrade cis-1,2-dichloroethylene (cis-DCE). The strains express the six genes of an evolved toluene ortho-monooxygenase from Burkholderia cepacia G4 (TOM-Green, which formed a reactive epoxide) with either (1) gamma-glutamylcysteine synthetase (GSHI...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr060008t
更新日期:2006-06-01 00:00:00
abstract::The honeybees Apis mellifera ligustica (Aml) and Apis cerana cerana (Acc) are two different western and eastern bee species that evolved in distinct ecologies and developed specific antennal olfactory systems for their survival. Knowledge of how their antennal olfactory systems function in regards to the success of ea...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr300298w
更新日期:2012-09-07 00:00:00
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journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/acs.jproteome.5b00764
更新日期:2015-11-06 00:00:00
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journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr070239f
更新日期:2007-10-01 00:00:00
abstract::Colon cancer is the most common cancer and the third leading cause of cancer mortality in humans. Using mass spectrometry-based metabolomics, the current study revealed the accumulation of four uremic toxins (cresol sulfate, cresol glucuronide, indoxyl sulfate, and phenyl sulfate) in the serum of mice harboring adenom...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr5013152
更新日期:2015-04-03 00:00:00
abstract::Correct phosphorylation site assignment is a critical aspect of phosphoproteomic analysis. Large-scale phosphopeptide data sets that are generated through liquid chromatography-coupled tandem mass spectrometry (LC-MS/MS) analysis often contain hundreds or thousands of phosphorylation sites that require validation. To ...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr800169k
更新日期:2008-07-01 00:00:00
abstract::Russell and colleagues deserve credit for being the first to use a QconCAT standard to simultaneously quantify both the wild-type and mutant peptides of a protein (i.e., CYP2B6) ( J. Proteome Res. 2013, 12 (12), 5934-5942. DOI: 10.1021/pr400279u). However, the rationale of their study was entirely different from ours ...
journal_title:Journal of proteome research
pub_type: 评论,杂志文章
doi:10.1021/acs.jproteome.8b00940
更新日期:2019-03-01 00:00:00
abstract::Ozonated autohemotherapy (O3-AHT) is a medical approach during which blood obtained from the patient is ozonated and injected back into the body. Despite an increasing number of evidence that O3-AHT is safe, this type of therapy remains controversial. To extend knowledge about the changes in blood evoked by O3-AHT, LC...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr3008946
更新日期:2012-12-07 00:00:00
abstract::Reference materials are vital to benchmarking the reproducibility of clinical tests and essential for monitoring laboratory performance for clinical proteomics. The reference material utilized for mass spectrometric analysis of the human proteome would ideally contain enough proteins to be suitably representative of t...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/acs.jproteome.8b00165
更新日期:2018-06-01 00:00:00
abstract::We describe a dual-column interface for parallel chromatography to improve throughput during LC-MS experimentation. The system employs a high-voltage switch to operate two capillary column/nanospray emitters fixed at the MS orifice. Sequentially loading one column while operating the second nearly doubles the LC-MS du...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr400738a
更新日期:2013-12-06 00:00:00
abstract::Traditional measures of short-term stress response such as fecal glucocorticoid metabolites (FGM) are widely used in controlled settings to quantify the intensity of stimulation to which cattle are exposed. However, FGMs present several methodological and interpretation pitfalls when applied on animals in free-ranging...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/acs.jproteome.9b00655
更新日期:2020-02-07 00:00:00
abstract::Populus tremula L. x P. alba L. (Populus x canescens (Aiton) Smith), clone INRA 717-1-B4, saplings were subjected to 120 ppb ozone exposure for 28 days. Chloroplasts were isolated, and the membrane proteins, solubilized using the detergent 1,2-diheptanoyl-sn-glycero-3-phosphocholine (DHPC), were analyzed in a differen...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr1012009
更新日期:2011-07-01 00:00:00
abstract::A biphasic monolithic capillary column with 10 cm segment of strong-cation exchange monolith and 65 cm segment of reversed-phase monolith was prepared within a single 100 microm i.d. capillary. Separation performance of this column was evaluated by a five-cycle online multidimensional separation of 10 microg tryptic d...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr700562b
更新日期:2008-01-01 00:00:00
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journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/acs.jproteome.8b00915
更新日期:2019-03-01 00:00:00
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journal_title:Journal of proteome research
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更新日期:2008-08-01 00:00:00
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journal_title:Journal of proteome research
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journal_title:Journal of proteome research
pub_type: 杂志文章
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更新日期:2016-04-01 00:00:00
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journal_title:Journal of proteome research
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doi:10.1021/pr4007574
更新日期:2014-02-07 00:00:00
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journal_title:Journal of proteome research
pub_type: 杂志文章
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更新日期:2006-05-01 00:00:00
abstract::The study of glycosylation patterns (glycomics) in biological samples is an emerging field that can provide key insights into cell development and pathology. A current challenge in the field of glycomics is to determine how to quantify changes in glycan expression between different cells, tissues, or biological fluids...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr070476i
更新日期:2008-01-01 00:00:00
abstract::Middle-aged and old left ventricles (LVs) are structurally and functionally very similar. Compared to a young LV, both show increased wall thickness and increased cavity size, with preserved cardiac function. However, when a stressor such as myocardial infarction occurs, striking differences are revealed between young...
journal_title:Journal of proteome research
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更新日期:2008-02-01 00:00:00
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更新日期:2014-11-07 00:00:00
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pub_type: 杂志文章
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更新日期:2009-10-01 00:00:00
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journal_title:Journal of proteome research
pub_type: 杂志文章
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更新日期:2014-02-07 00:00:00