Abstract:
:Transferrin purification from mice serum samples by immunoaffinity chromatography (IAC) was optimized in order to study the possible modifications occurring in its glycans in collagen-induced arthritis (CIA) samples. SDS-PAGE and nanoLC-MS/MS were used to monitor the IAC purification performance. Afterward, a relative quantification of mouse transferrin (mTf) glycan isomers using [12C6]/[13C6]-aniline was used to unequivocally detect alterations in the glycan profile of CIA mice. In addition, multivariate data analysis was applied to identify the most meaningful glycan isomers for the discrimination between control and pathological samples. Partial least-squares discriminant analysis (PLS-DA) revealed that five out of fifteen mTf glycan isomers could be potential biomarkers of CIA, most of them corresponding to highly sialylated structures (H6N5S3_2, H6N5S3_3, and H5N4S3_2). Moreover, some of these glycan isomers also seemed to be related with the progression of CIA, especially H6N5S2 and H6N5S3_2, as their overexpression increased with the clinical score of the pathology. Hence, the established methodology not only provides valuable information to find glycan-based biomarkers of CIA, but also leaves the door open to evaluate, in the future, glycosylation changes of many other inflammatory diseases, in which transferrin has been described to be altered.
journal_name
J Proteome Resjournal_title
Journal of proteome researchauthors
Mancera-Arteu M,Giménez E,Sancho J,Sanz-Nebot Vdoi
10.1021/acs.jproteome.0c00016subject
Has Abstractpub_date
2020-04-03 00:00:00pages
1750-1759issue
4eissn
1535-3893issn
1535-3907journal_volume
19pub_type
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journal_title:Journal of proteome research
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pub_type: 杂志文章
doi:10.1021/pr4009404
更新日期:2014-04-04 00:00:00
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journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr100394u
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journal_title:Journal of proteome research
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更新日期:2009-01-01 00:00:00
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journal_title:Journal of proteome research
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doi:10.1021/acs.jproteome.8b00615
更新日期:2019-02-01 00:00:00
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journal_title:Journal of proteome research
pub_type: 杂志文章
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journal_title:Journal of proteome research
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