Abstract:
:We describe Peptoonist, a program that can automatically identify the glycans (sugars) present at each N-glycosylation site of a protein. The input to Peptoonist is a series of mass spectra, both MS and MS/MS, obtained from a liquid chromatography (LC) run of proteolytically digested purified glycoproteins. The program uses MS/MS to identify glycosylated peptides and single-MS to identify the N-glycans present on each of these peptides, at least to the level of monosaccharide composition. We validate the program on an LC run of mouse zona pellucida proteins that had been intensively hand annotated by a human expert. Our program doubled the number of glycopeptide identifications, and also found several possible errors in the hand annotation. In addition, it automatically made most of the same glycan isomer identifications as the expert annotator.
journal_name
J Proteome Resjournal_title
Journal of proteome researchauthors
Goldberg D,Bern M,Parry S,Sutton-Smith M,Panico M,Morris HR,Dell Adoi
10.1021/pr070239fsubject
Has Abstractpub_date
2007-10-01 00:00:00pages
3995-4005issue
10eissn
1535-3893issn
1535-3907journal_volume
6pub_type
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