Abstract：
:Pancreatic cystic neoplasms represent 10-15% of primary cystic masses of the pancreas. While pancreatic cysts are detected with an increasing frequency due to the use of advanced imaging modalities in clinical practice, the diagnosis of pancreatic cystic neoplasms remains unsatisfactory because available diagnostic techniques proved not sensitive enough so far. This study was designed to characterize the proteomic pattern of pancreatic cyst fluids obtained from various cystic lesions. Cyst fluids were collected by direct puncture during open surgery to avoid any possible contamination from other tissues. CEA, CA-19-9, and amylase concentrations were measured using specific immunoassays. After immunodepletion and fractionation by SDS-PAGE, proteins were digested and analyzed by LC-MS/MS. Specific histological lesions were found to be associated with distinct protein patterns. Interestingly, some of these proteins have been proposed as biomarkers of pancreatic cancer. Immunoblots allowed for verifying the differential expression in specific cyst fluids of two selected proteins, olfactomedin-4 and mucin-18. Finally, immunohistochemistry was performed to correlate these data with the expression pattern of olfactomedin-4 and mucin-18 in pancreatic cyst tissues. Results from this study indicate that proteomic analysis of cyst fluid could provide reliable candidates for developing new biomarkers for the preoperative management of malignant and premalignant pancreatic cysts.
journal_name
J Proteome Resjournal_title
Journal of proteome researchauthors
Cuoghi A,Farina A,Z'graggen K,Dumonceau JM,Tomasi A,Hochstrasser DF,Genevay M,Lescuyer P,Frossard JLdoi
10.1021/pr2000557subject
Has Abstractpub_date
2011-05-06 00:00:00pages
2664-70issue
5eissn
1535-3893issn
1535-3907journal_volume
10pub_type
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journal_title：Journal of proteome research
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journal_title：Journal of proteome research
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更新日期：2013-09-06 00:00:00
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journal_title：Journal of proteome research
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pub_type： 杂志文章
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更新日期：2005-11-01 00:00:00
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journal_title：Journal of proteome research
pub_type： 信件
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更新日期：2004-09-01 00:00:00
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journal_title：Journal of proteome research
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更新日期：2004-11-01 00:00:00
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journal_title：Journal of proteome research
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更新日期：2004-09-01 00:00:00
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journal_title：Journal of proteome research
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更新日期：2014-10-03 00:00:00
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journal_title：Journal of proteome research
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doi：10.1021/acs.jproteome.7b00784
更新日期：2018-03-02 00:00:00
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journal_title：Journal of proteome research
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journal_title：Journal of proteome research
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更新日期：2011-01-07 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
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更新日期：2009-01-01 00:00:00
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journal_title：Journal of proteome research
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更新日期：2007-02-01 00:00:00
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journal_title：Journal of proteome research
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journal_title：Journal of proteome research
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更新日期：2015-09-04 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr1009383
更新日期：2011-03-04 00:00:00
