Novel highly sensitive, specific, and straightforward strategy for comprehensive N-terminal proteomics reveals unknown substrates of the mitochondrial peptidase Icp55.

abstract：:The development of plasma biomarkers has proven to be more challenging than initially anticipated. Many studies have reported lists of candidate proteins rather than validated candidate markers with an assigned performance to a specific clinical objective. Biomarker research necessitates a clear rational framework wit...

journal_title：Journal of proteome research

authors： Surinova S,Schiess R,Hüttenhain R,Cerciello F,Wollscheid B,Aebersold R

更新日期：2011-01-07 00:00:00

abstract：:Chronic kidney disease (CKD) has emerged as a major public health problem worldwide. It frequently progresses to end-stage renal disease, which is related to very high cost and mortality. Novel biomarkers can provide insight into the novel mechanism, facilitate early detection, and monitor progression of CKD and its r...

journal_title：Journal of proteome research

authors： Zhang ZH,Chen H,Vaziri ND,Mao JR,Zhang L,Bai X,Zhao YY

更新日期：2016-10-07 00:00:00

abstract：:Proteomics by mass spectrometry (MS) allows the large-scale identification and quantitation of the cellular proteins in a given biological context. Systems biology studies from proteomics data are largely limited by the accuracy and coverage of quantitative proteomics along with missing values. Toward this end, statis...

journal_title：Journal of proteome research

authors： Aggarwal S,Talukdar NC,Yadav AK

更新日期：2019-06-07 00:00:00

abstract：:Mass spectrometry is the main analytical technique currently used to address the challenges of glycomics as it offers unrivalled levels of sensitivity and the ability to handle complex mixtures of different glycan variations. Determination of glycan structures from analysis of MS data is a major bottleneck in high-thr...

journal_title：Journal of proteome research

authors： Ceroni A,Maass K,Geyer H,Geyer R,Dell A,Haslam SM

更新日期：2008-04-01 00:00:00

abstract：:Transient cerebral ischemia dramatically activates small ubiquitin-like modifier (SUMO2/3) conjugation. In cells exposed to 6 h of transient oxygen/glucose deprivation (OGD), a model of ischemia, SUMOylation increases profoundly between 0 and 30 min following re-oxygenation. To elucidate the effect of transient OGD on...

journal_title：Journal of proteome research

authors： Yang W,Thompson JW,Wang Z,Wang L,Sheng H,Foster MW,Moseley MA,Paschen W

更新日期：2012-02-03 00:00:00

abstract：:Post-translational nitration of proteins was analyzed by capillary reversed-phase high-performance liquid chromatography (RP-HPLC) on-line interfaced to electrospray ionization mass spectrometry (ESI--MS) or tandem mass spectrometry (ESI--MS/MS). Both methods were compared using a tryptic digest of bovine serum albumi...

journal_title：Journal of proteome research

authors： Walcher W,Franze T,Weller MG,Pöschl U,Huber CG

更新日期：2003-09-01 00:00:00

abstract：:Fast (120 s) and high-throughput (more than six samples at once) in-gel trypsin digestion of proteins using sonoreactor technology has been achieved. Successful protein identification was done by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, MALDI-TOF-MS. Specific identification of the ...

journal_title：Journal of proteome research

authors： Rial-Otero R,Carreira RJ,Cordeiro FM,Moro AJ,Fernandes L,Moura I,Capelo JL

更新日期：2007-02-01 00:00:00

abstract：:Hypoxia inducible factor (HIF)-2α protein expression in solid tumors promotes stem-like phenotype in cancer stem cells and increases tumorigenic potential in nonstem cancer cells. Recently, we have shown that HIF-1/2α gene expression is correlated to neuroblastoma (NB) poor survival and to undifferentiated tumor state...

journal_title：Journal of proteome research

authors： Cimmino F,Pezone L,Avitabile M,Persano L,Vitale M,Sassi M,Bresolin S,Serafin V,Zambrano N,Scaloni A,Basso G,Iolascon A,Capasso M

更新日期：2016-10-07 00:00:00

abstract：:Human milk oligosaccharides (HMOs) perform a number of functions including serving as prebiotics to stimulate the growth of beneficial intestinal bacteria, as receptor analogues to inhibit binding of pathogens, and as substances that promote postnatal brain development. There is further evidence that HMOs participate ...

journal_title：Journal of proteome research

authors： Wu S,Tao N,German JB,Grimm R,Lebrilla CB

更新日期：2010-08-06 00:00:00

abstract：:Various mammalian cells including tumor cells secrete extracellular vesicles (EVs), otherwise known as exosomes and microvesicles. EVs are nanosized bilayered proteolipids and play multiple roles in intercellular communication. Although many vesicular proteins have been identified, their functional interrelationships ...

journal_title：Journal of proteome research

authors： Choi DS,Yang JS,Choi EJ,Jang SC,Park S,Kim OY,Hwang D,Kim KP,Kim YK,Kim S,Gho YS

更新日期：2012-02-03 00:00:00

abstract：:Human plasma and serum proteins are subject to intrinsic proteolytic degradation both during and after blood collection. By monitoring peptides, we investigated the stability of plasma and serum samples and the effects of anticoagulants and protease inhibitors on the plasma samples. Serum and plasma were subjected to ...

journal_title：Journal of proteome research

authors： Yi J,Kim C,Gelfand CA

更新日期：2007-05-01 00:00:00

abstract：:The secreted proteins of bacteria are usually accompanied by virulence factors, which can cause inflammation and damage host cells. Identifying the secretomes arising from the interactions of bacteria and host cells could therefore increase understanding of the mechanisms during initial pathogenesis. The present study...

journal_title：Journal of proteome research

authors： Chiu KH,Wang LH,Tsai TT,Lei HY,Liao PC

更新日期：2017-01-06 00:00:00

abstract：:Serum protein profiling by mass spectrometry is a promising method for early detection of cancer. We have implemented a combined strategy based on matrix-assisted laser desorption ionization mass spectrometry (MALDI MS) and statistical data analysis for serum protein profiling and applied it in a well-described breast...

journal_title：Journal of proteome research

authors： Callesen AK,Vach W,Jørgensen PE,Cold S,Tan Q,Depont Christensen R,Mogensen O,Kruse TA,Jensen ON,Madsen JS

更新日期：2008-04-01 00:00:00

abstract：:The zebrafish genome has recently been sequenced and annotated allowing for high-throughput proteomic analysis. Here, we report for the first time a proteomic subset of zebrafish liver, an important organ for metabolizing toxins. Using a newly developed analytical procedure, we have identified 1204 proteins from the c...

journal_title：Journal of proteome research

authors： Wang N,Mackenzie L,De Souza AG,Zhong H,Goss G,Li L

更新日期：2007-01-01 00:00:00

abstract：:Periodontal disease is perhaps the most common infectious disease in humans. Gingival crevicular fluid (GCF) is a local inflammatory exudate of the periodontal tissues. Its composition greatly varies between health and periodontal disease. GCF collection is rapid and noninvasive, but previous approaches aiming to anal...

journal_title：Journal of proteome research

authors： Bostanci N,Heywood W,Mills K,Parkar M,Nibali L,Donos N

更新日期：2010-05-07 00:00:00

abstract：:Many tumors display significant cellular heterogeneity as well as molecular heterogeneity. Sensitive biomarkers that differentiate between diagnostically challenging tumors must contend with this heterogeneity. Mass spectrometry-based molecular histology of a patient series of heterogeneous, microscopically identical ...

journal_title：Journal of proteome research

authors： Jones EA,Schmitz N,Waaijer CJ,Frese CK,van Remoortere A,van Zeijl RJ,Heck AJ,Hogendoorn PC,Deelder AM,Altelaar AF,Bovée JV,McDonnell LA

更新日期：2013-04-05 00:00:00

abstract：:Schwann cells (SCs) are the principal glial cells of the peripheral nervous system (PNS). As a result of tissue heterogeneity and difficulties in the isolation and culture of primary SCs, a considerable understanding of SC biology is obtained from SC lines. However, the differences between the primary SCs and SC lines...

journal_title：Journal of proteome research

authors： Ji Y,Shen M,Wang X,Zhang S,Yu S,Chen G,Gu X,Ding F

更新日期：2012-06-01 00:00:00

abstract：:Aquaporin (AQP) family plays a pivotal role in fluid secretion and absorption, especially in the digestive system and secretory glands. Within this family, AQP8 was reported to be widely expressed in the epithelia of the digestive tract, liver, and pancreas. In two parallel experimental platforms with different analyt...

journal_title：Journal of proteome research

authors： Magdeldin S,Li H,Yoshida Y,Satokata I,Maeda Y,Yokoyama M,Enany S,Zhang Y,Xu B,Fujinaka H,Yaoita E,Yamamoto T

更新日期：2010-12-03 00:00:00

abstract：:Rapid early triage and dose estimation is vital for limited medical resource allocation and treatment of a large number of the wounded after radiological accidents. Lipidomics has been utilized to delineate biofluid lipid signatures after irradiation. Here, high-coverage targeted lipidomics was employed to screen radi...

journal_title：Journal of proteome research

authors： Xi C,Zhao H,Lu X,Cai TJ,Li S,Liu KH,Tian M,Liu QJ

更新日期：2021-01-01 00:00:00

abstract：:Interleukin-10 is an immunosuppressive cytokine involved in the regulation of gastrointestinal mucosal immunity toward intestinal microbiota. Interleukin-10-deficient (IL10(-/-)) mice develop Crohn's disease-like colitis unless raised in germ-free conditions. Previous gas chromatography-mass spectrometry (GC-MS) metab...

journal_title：Journal of proteome research

authors： Lin HM,Barnett MP,Roy NC,Joyce NI,Zhu S,Armstrong K,Helsby NA,Ferguson LR,Rowan DD

更新日期：2010-04-05 00:00:00

abstract：:12-O-Tetradecanoyl-phorbol-13-acetate (TPA) is a plant derivative with multiple function as tumor promoter, differentiation revulsant or leukemia therapy drug. The molecular mechanism of its function is perplexing. Many studies have focused on the mechanism of TPA stimulation in tumor promotion of mouse models or term...

journal_title：Journal of proteome research

authors： Jiang P,Gan M,Huang H,Shen X,Wang S,Yao K

更新日期：2005-03-01 00:00:00

abstract：:The term "spectral clustering" is sometimes used to refer to the clustering of mass spectrometry data. However, it also classically refers to a family of popular clustering algorithms. To avoid confusion, a more specific term could advantageously be coined. ...

journal_title：Journal of proteome research

authors： Borges H,Guibert R,Permiakova O,Burger T

更新日期：2019-01-04 00:00:00

abstract：:In systemic amyloidosis, accumulation of misfolded proteins as extracellular amyloid fibrils in tissues causes severe organ dysfunction, but the molecular events of tissue damage related to amyloid deposition are still largely unknown. Through the use of the MudPIT proteomic approach, comprehensive protein profiles of...

journal_title：Journal of proteome research

authors： Brambilla F,Lavatelli F,Di Silvestre D,Valentini V,Palladini G,Merlini G,Mauri P

更新日期：2013-12-06 00:00:00

abstract：:Acute kidney injury (AKI) is a frequent complication after cardiopulmonary bypass, but early detection of postoperative AKI remains challenging. Protein biomarkers predict AKI excellently in homogeneous cohorts but are less reliable in patients suffering from various comorbidities. We employed nuclear magnetic resonan...

journal_title：Journal of proteome research

authors： Zacharias HU,Hochrein J,Vogl FC,Schley G,Mayer F,Jeleazcov C,Eckardt KU,Willam C,Oefner PJ,Gronwald W

更新日期：2015-07-02 00:00:00

abstract：:Various biological activities of immunoglobulin G (IgG) including antibody-dependent cellular cytotoxicity (ADCC) are modulated by the structural features of the N-glycans in the Fc part. In this study, we describe a population of IgG1 alloantibodies which are formed during pregnancy against human platelet antigens (H...

journal_title：Journal of proteome research

authors： Wuhrer M,Porcelijn L,Kapur R,Koeleman CA,Deelder A,de Haas M,Vidarsson G

更新日期：2009-02-01 00:00:00

abstract：:Cytological examination of cells from bronchoalveolar lavage (BAL) is commonly used for the diagnosis of lung cancer. Proteins released from lung cancer cells into BAL may serve as biomarkers for cancer detection. In this study, N-glycoproteins in eight cases of BAL fluid, as well as eight lung adenocarcinoma tissues ...

journal_title：Journal of proteome research

authors： Li QK,Shah P,Li Y,Aiyetan PO,Chen J,Yung R,Molena D,Gabrielson E,Askin F,Chan DW,Zhang H

更新日期：2013-08-02 00:00:00

abstract：:Advancements in studies on the evolutionary mechanisms underlying bacterial antibiotic resistance are unclear. This study aimed to investigate the evolutionary mechanism underlying bacterial antibiotic resistance using isobaric tags for relative and absolute quantitation-based quantitative proteomics along with functi...

journal_title：Journal of proteome research

authors： Du GF,Zheng YD,Chen J,He QY,Sun X

更新日期：2019-11-01 00:00:00

abstract：:The energy-dissipating alternative oxidase (AOX) from Hansenula anomala was expressed in Saccharomyces cerevisiae. The recombinant AOX was functional. A comparative analysis by two-dimensional differential in-gel electrophoresis (2D-DIGE) of mitochondrial protein patterns found in wild-type and recombinant AOX strains...

journal_title：Journal of proteome research

authors： Mathy G,Navet R,Gerkens P,Leprince P,De Pauw E,Sluse-Goffart CM,Sluse FE,Douette P

更新日期：2006-02-01 00:00:00

abstract：:The chromatophore membrane of the photosynthetic diazotroph Rhodospirillum rubrum is of vital importance for a number of central processes, including nitrogen fixation. Using a novel amphiphile, we have identified protein complexes present under different nitrogen availability conditions by the use of two-dimensional ...

journal_title：Journal of proteome research

authors： Selao TT,Branca R,Chae PS,Lehtiö J,Gellman SH,Rasmussen SG,Nordlund S,Norén A

更新日期：2011-06-03 00:00:00

abstract：:Accurate predictions of peptide retention times (RT) in liquid chromatography have many applications in mass spectrometry-based proteomics. Most notably such predictions are used to weed out incorrect peptide-spectrum matches, and to design targeted proteomics experiments. In this study, we describe a RT predictor, EL...

journal_title：Journal of proteome research

authors： Moruz L,Tomazela D,Käll L

更新日期：2010-10-01 00:00:00