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The protein interaction network of extracellular vesicles derived from human colorectal cancer cells.

Abstract：

:Various mammalian cells including tumor cells secrete extracellular vesicles (EVs), otherwise known as exosomes and microvesicles. EVs are nanosized bilayered proteolipids and play multiple roles in intercellular communication. Although many vesicular proteins have been identified, their functional interrelationships and the mechanisms of EV biogenesis remain unknown. By interrogating proteomic data using systems approaches, we have created a protein interaction network of human colorectal cancer cell-derived EVs which comprises 1491 interactions between 957 vesicular proteins. We discovered that EVs have well-connected clusters with several hub proteins similar to other subcellular networks. We also experimentally validated that direct protein interactions between cellular proteins may be involved in protein sorting during EV formation. Moreover, physically and functionally interconnected protein complexes form functional modules involved in EV biogenesis and functions. Specifically, we discovered that SRC signaling plays a major role in EV biogenesis, and confirmed that inhibition of SRC kinase decreased the intracellular biogenesis and cell surface release of EVs. Our study provides global insights into the cargo-sorting, biogenesis, and pathophysiological roles of these complex extracellular organelles.

journal_name

J Proteome Res

journal_title

Journal of proteome research

authors

Choi DS,Yang JS,Choi EJ,Jang SC,Park S,Kim OY,Hwang D,Kim KP,Kim YK,Kim S,Gho YS

doi

10.1021/pr200842h

subject

Has Abstract

pub_date

2012-02-03 00:00:00

pages

1144-51

issue

2

eissn

1535-3893

issn

1535-3907

journal_volume

11

pub_type

杂志文章

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