Novel Mechanistic Insights into Bacterial Fluoroquinolone Resistance.

abstract：:Recent studies have shown a remarkable degree of plasticity in the N-glycome of the model nematode Caenorhabditis elegans; ablation of glycosylation-relevant genes can result in radically altered N-glycan profiles despite only minor biological phenotypic effects. Up to four fucose residues and five different linkages ...

journal_title：Journal of proteome research

authors： Yan S,Jin C,Wilson IB,Paschinger K

更新日期：2015-12-04 00:00:00

abstract：:The Chromosome-Centric Human Proteome Project (C-HPP) aims to identify "missing" proteins in the neXtProt knowledgebase. We present an in-depth proteomics analysis of the human sperm proteome to identify testis-enriched missing proteins. Using protein extraction procedures and LC-MS/MS analysis, we detected 235 protei...

journal_title：Journal of proteome research

authors： Vandenbrouck Y,Lane L,Carapito C,Duek P,Rondel K,Bruley C,Macron C,Gonzalez de Peredo A,Couté Y,Chaoui K,Com E,Gateau A,Hesse AM,Marcellin M,Méar L,Mouton-Barbosa E,Robin T,Burlet-Schiltz O,Cianferani S,Ferro M,Fr

更新日期：2016-11-04 00:00:00

abstract：:Tropomyosins (Tm) constitute a family of ubiquitous and highly conserved actin-binding proteins, playing essential roles in a variety of biological processes. Tm isoforms produced by multiple Tm encoding genes and alternatively expressed exons along with post-translational modifications (PTMs) regulate Tm function. Th...

journal_title：Journal of proteome research

authors： Peng Y,Chen X,Zhang H,Xu Q,Hacker TA,Ge Y

更新日期：2013-01-04 00:00:00

abstract：:Affinity purification coupled to 1-D gel-free liquid chromatography mass spectrometry (LC-MS) is a well-established and widespread approach for the analyses of noncovalently interacting protein complexes. In this study, two proteins conjugated to a streptavidin-binding peptide and hemagglutinin double tag were express...

journal_title：Journal of proteome research

authors： Huber ML,Sacco R,Parapatics K,Skucha A,Khamina K,Müller AC,Rudashevskaya EL,Bennett KL

更新日期：2014-02-07 00:00:00

abstract：:Liquid chromatography in coupling with high-resolution ESI-LTQ-Orbitrap mass spectrometry was applied for a proteomic study of pediatric pilocytic astrocytoma brain tumor intracystic fluid by an integrated top-down/bottom-up platform. Both of the proteomic strategies resulted complementary and support each other in co...

journal_title：Journal of proteome research

authors： Inserra I,Iavarone F,Martelli C,D'Angelo L,Delfino D,Rossetti DV,Tamburrini G,Massimi L,Caldarelli M,Di Rocco C,Messana I,Castagnola M,Desiderio C

更新日期：2014-11-07 00:00:00

abstract：:In this paper, we describe a method for analyzing large-volume protein samples using capillary electrophoresis in conjunction with laser-induced fluorescence detection (CE-LIF). To improve the stacking and separation efficiencies of proteins, we added either 0.01% sodium dodecyl sulfate (SDS) or 0.01% poly(ethylene ox...

journal_title：Journal of proteome research

authors： Huang YF,Hsieh MM,Tseng WL,Chang HT

更新日期：2006-02-01 00:00:00

abstract：:Glial-cell-line-derived neurotrophic factor (GDNF) is a most potent survival factor for dopaminergic neurons. In addition, GDNF was also found to promote neurite outgrowth in dopaminergic neurons. However, despite the potential clinical and physiological importance of GDNF, its mechanism of action is unclear. Therefor...

journal_title：Journal of proteome research

authors： Hong Z,Zhang QY,Liu J,Wang ZQ,Zhang Y,Xiao Q,Lu J,Zhou HY,Chen SD

更新日期：2009-06-01 00:00:00

abstract：:Cellular functions are largely carried out by noncovalent protein complexes that may exist within the cell as stable modules or as assemblies of dynamically changing composition, whose formation and decomposition are triggered in response to extracellular stimuli. The protein constituents of complexes often exhibit po...

journal_title：Journal of proteome research

authors： Przybylski C,Jünger MA,Aubertin J,Radvanyi F,Aebersold R,Pflieger D

更新日期：2010-10-01 00:00:00

abstract：:Natural disasters such as drought, extreme temperatures, and flooding can severely impact crop production. Understanding the metabolic response of crops threatened with these disasters provides insights into biological response mechanisms that can influence survival. In this study, a comparative analysis of GC-MS and ...

journal_title：Journal of proteome research

authors： Barding GA Jr,Béni S,Fukao T,Bailey-Serres J,Larive CK

更新日期：2013-02-01 00:00:00

abstract：:In this study, a tandem LC-MS (Waters Xevo TQ) MRM-based MS method was developed for rapid, broad profiling of hydrophilic metabolites from biological samples, in either positive or negative ion modes without the need for an ion pairing reagent, using a reversed-phase pentafluorophenylpropyl (PFPP) column. The develop...

journal_title：Journal of proteome research

authors： Lv H,Palacios G,Hartil K,Kurland IJ

更新日期：2011-04-01 00:00:00

abstract：:Metal based chemotherapeutic drugs are widely used as an effective method to defeat various cancers. In this study, the mechanism of action of a novel therapeutic agent, [Pd(sac)(terpy)](sac)·4H2O (sac = saccharinate, and terpy = 2,2':6',2″-terpyridine) was studied. To better understand the proteomic changes in respon...

journal_title：Journal of proteome research

authors： Adiguzel Z,Baykal AT,Kacar O,Yilmaz VT,Ulukaya E,Acilan C

更新日期：2014-11-07 00:00:00

abstract：:As spectral library searching has received increasing attention for peptide identification, constructing good decoy spectra from the target spectra is the key to correctly estimating the false discovery rate in searching against the concatenated target-decoy spectral library. Several methods have been proposed to cons...

journal_title：Journal of proteome research

authors： Cheng CY,Tsai CF,Chen YJ,Sung TY,Hsu WL

更新日期：2013-05-03 00:00:00

abstract：:Energy imbalance is a primary cause of obesity. While the classical approach to attenuate weight gain includes an increase in energy expenditure through exercise, dietary manipulation such as the inclusion of dairy products has also been proven effective. In the present study, we explored the potential mechanisms by w...

journal_title：Journal of proteome research

authors： Mayengbam S,Mickiewicz B,Trottier SK,Mu C,Wright DC,Reimer RA,Vogel HJ,Shearer J

更新日期：2019-11-01 00:00:00

abstract：:As a recently validated reversible post translational modification, lysine malonylation regulates diverse cellular processes from bacteria to mammals, but its existence and function in photosynthetic organisms remain unknown. Cyanobacteria are the most ancient group of photosynthetic prokaryotes and contribute about 5...

journal_title：Journal of proteome research

authors： Ma Y,Yang M,Lin X,Liu X,Huang H,Ge F

更新日期：2017-05-05 00:00:00

abstract：:A novel metabolite identification strategy is presented for the combined NMR/MS analysis of complex metabolite mixtures. The approach first identifies metabolite candidates from 1D or 2D NMR spectra by NMR database query, which is followed by the determination of the masses (m/z) of their possible ions, adducts, fragm...

journal_title：Journal of proteome research

authors： Bingol K,Brüschweiler R

更新日期：2015-06-05 00:00:00

abstract：:Src family kinases (SFKs) are the earliest known family of tyrosine kinases and are widely thought to play essential roles in cellular signal transduction. Although numerous functional analyses have been performed, no study has analyzed the specificity of all SFKs on an equal platform. To gain a better understanding o...

journal_title：Journal of proteome research

authors： Takeda H,Kawamura Y,Miura A,Mori M,Wakamatsu A,Yamamoto J,Isogai T,Matsumoto M,Nakayama KI,Natsume T,Nomura N,Goshima N

更新日期：2010-11-05 00:00:00

abstract：:Proteomics data analysis strongly benefits from not studying single proteins in isolation but taking their multivariate interdependence into account. We introduce PerseusNet, the new Perseus network module for the biological analysis of proteomics data. Proteomics is commonly used to generate networks, e.g., with affi...

journal_title：Journal of proteome research

authors： Rudolph JD,Cox J

更新日期：2019-05-03 00:00:00

abstract：:Accurate and sensitive detection of protein-protein and protein-RNA interactions is key to understanding their biological functions. Traditional methods to identify these interactions require cell lysis and biochemical manipulations that exclude cellular compartments that cannot be solubilized under mild conditions. H...

journal_title：Journal of proteome research

authors： Beck DB,Narendra V,Drury WJ 3rd,Casey R,Jansen PW,Yuan ZF,Garcia BA,Vermeulen M,Bonasio R

更新日期：2014-12-05 00:00:00

abstract：:The exact impact of bariatric surgery in metabolically "healthy" (MH) or "unhealthy" (MU) phenotypes for the study of the metabolic improvement is still unknown. We applied an untargeted LC-ESI-TripleTOF-MS-driven metabolomics approach in serum samples from 39 patients with morbid obesity (MH and MU) 1, 3, and 6 month...

journal_title：Journal of proteome research

authors： Palau-Rodriguez M,Tulipani S,Marco-Ramell A,Miñarro A,Jauregui O,Gonzalez-Dominguez R,Sanchez-Pla A,Ramos-Molina B,Tinahones FJ,Andres-Lacueva C

更新日期：2018-08-03 00:00:00

abstract：:Proteins and peptides present within clinical samples represent a valuable library of information regarding the ongoing processes within cells and tissues in health and disease. We have developed and validated novel technology applications that can be used to characterize the patterns of global protein expression in t...

journal_title：Journal of proteome research

authors： Marko-Varga G,Lindberg H,Löfdahl CG,Jönsson P,Hansson L,Dahlbäck M,Lindquist E,Johansson L,Foster M,Fehniger TE

更新日期：2005-07-01 00:00:00

abstract：:We assessed proteomic patterns in breast cancer using MALDI MS and laser capture microdissected cells. Protein and peptide expression in invasive mammary carcinoma versus normal mammary epithelium and estrogen-receptor positive versus estrogen-receptor negative tumors were compared. Biomarker candidates were identifie...

journal_title：Journal of proteome research

authors： Sanders ME,Dias EC,Xu BJ,Mobley JA,Billheimer D,Roder H,Grigorieva J,Dowsett M,Arteaga CL,Caprioli RM

更新日期：2008-04-01 00:00:00

abstract：:Human saliva is a protein-rich, easily accessible source of potential local and systemic biomarkers to monitor changes that occur under pathological conditions; however, little is known about the changes in abundance associated with normal aging. In this study, we performed a comprehensive proteomic profiling of poole...

journal_title：Journal of proteome research

authors： Ambatipudi KS,Lu B,Hagen FK,Melvin JE,Yates JR

更新日期：2009-11-01 00:00:00

abstract：:Immune recognition of nonself is coordinated through complex mechanisms involving both innate and adaptive responses. Circulating antibodies communicate with effector cells of the innate immune system through surface receptors known as Fcγ receptors (FcγRs). The FcγRs are single-pass transmembrane glycoproteins respon...

journal_title：Journal of proteome research

authors： Cosgrave EF,Struwe WB,Hayes JM,Harvey DJ,Wormald MR,Rudd PM

更新日期：2013-08-02 00:00:00

abstract：:Ricin is a type II ribosome-inactivating protein toxin consisting of A and B chains linked by one interchain disulfide bond. Because of its high toxicity depending on both chains together, confirming the presence of both A and B chains of intact ricin is required during the investigation of the illegal production and ...

journal_title：Journal of proteome research

authors： Liu CC,Liang LH,Yang Y,Yu HL,Yan L,Li XS,Chen B,Liu SL,Xi HL

更新日期：2021-01-01 00:00:00

abstract：:Crohn's disease is an inflammatory disorder of the bowel, believed to arise from the dysregulation of intestinal mucosal immunity. The interleukin-10-deficient (IL10-/-) mouse, which develops intestinal inflammation in the presence of gut microflora, serves as a mouse model of Crohn's disease. Nontargeted urinary meta...

journal_title：Journal of proteome research

authors： Lin HM,Edmunds SI,Helsby NA,Ferguson LR,Rowan DD

更新日期：2009-04-01 00:00:00

abstract：:Yellow fever virus (YFV) replication is highly dependent on host cell factors. YFV NS4B is reported to be involved in viral replication and immune evasion. Here interactions between NS4B and human proteins were determined using a GST pull-down assay and analyzed using 1-DE and LC-MS/MS. We present a total of 207 prote...

journal_title：Journal of proteome research

authors： Vidotto A,Morais AT,Ribeiro MR,Pacca CC,Terzian AC,Gil LH,Mohana-Borges R,Gallay P,Nogueira ML

更新日期：2017-04-07 00:00:00

abstract：:Cytological examination of cells from bronchoalveolar lavage (BAL) is commonly used for the diagnosis of lung cancer. Proteins released from lung cancer cells into BAL may serve as biomarkers for cancer detection. In this study, N-glycoproteins in eight cases of BAL fluid, as well as eight lung adenocarcinoma tissues ...

journal_title：Journal of proteome research

authors： Li QK,Shah P,Li Y,Aiyetan PO,Chen J,Yung R,Molena D,Gabrielson E,Askin F,Chan DW,Zhang H

更新日期：2013-08-02 00:00:00

abstract：:A "one-pot" alternative method for processing proteins and isolating peptide mixtures from bacterial samples is presented for liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis and data reduction. The conventional in-solution digestion of the protein contents of bacteria is compared to a small disposab...

journal_title：Journal of proteome research

authors： Jabbour RE,Deshpande SV,Stanford MF,Wick CH,Zulich AW,Snyder AP

更新日期：2011-02-04 00:00:00

abstract：:Chinese hamster ovary (CHO) cell lines are widely used for the expression of therapeutic recombinant proteins, including monoclonal antibodies and other biologics. For manufacturing, cells derived from a single-cell clone are typically used to ensure product consistency. Presently, fetal bovine serum (FBS) is commonly...

journal_title：Journal of proteome research

authors： Lim UM,Yap MG,Lim YP,Goh LT,Ng SK

更新日期：2013-07-05 00:00:00

abstract：:Glioblastoma multiforme (GBM) is the most aggressive malignant primary brain tumor, with a dismal mean survival even with the current standard of care. Although in vitro cell systems can provide mechanistic insight into the regulatory networks governing GBM cell proliferation and migration, clinical samples provide a ...

journal_title：Journal of proteome research

authors： Johnson H,White FM

更新日期：2014-11-07 00:00:00