Abstract：
:During the development of cancer, changes in cellular glycosylation are observed, indicating that alterations of the glycome occur in extracellular fluids as well as in serum and could therefore serve as tumor biomarkers. In the case of epithelial ovarian cancer (EOC), common tumor markers such as CA125 are known to have poor specificity; therefore, better biomarkers are needed. The aim of this work was to identify new potential glycan biomarkers in EOC-patients. N-Glycans were cleaved from serum glycoproteins from 63 preoperative primary EOC-patients along with 33 age-matched healthy women, permethylated, and analyzed using MALDI-TOF mass spectrometry. A value named GLYCOV was calculated from the relative areas of the 11 N-glycan biomarkers revealed by SPSS statistical analyses, namely four high-mannose and seven complex-type fucosylated N-glycans. GLYCOV diagnosed primary EOC with a sensitivity of 97% and a specificity of 98.4% whereas CA-125 showed a sensitivity of 97% and a specificity of 88.9%. Our study is the first one to compare glycan values with the established tumor marker CA125 and to give better results. Therefore, the N-glycome could potentially be used as a biomarker.
journal_name
J Proteome Resjournal_title
Journal of proteome researchauthors
Biskup K,Braicu EI,Sehouli J,Fotopoulou C,Tauber R,Berger M,Blanchard Vdoi
10.1021/pr400405xsubject
Has Abstractpub_date
2013-09-06 00:00:00pages
4056-63issue
9eissn
1535-3893issn
1535-3907journal_volume
12pub_type
杂志文章abstract：:Gaining insights into the molecular events that govern the progression from melanoma in situ to advanced melanoma and understanding how the local microenvironment at the melanoma site influences this progression are two clinically pivotal aspects that to date are largely unexplored. In an effort to identify key regula...
journal_title：Journal of proteome research
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更新日期：2008-11-01 00:00:00
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更新日期：2012-02-03 00:00:00
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