Abstract：
:A biphasic monolithic capillary column with 10 cm segment of strong-cation exchange monolith and 65 cm segment of reversed-phase monolith was prepared within a single 100 microm i.d. capillary. Separation performance of this column was evaluated by a five-cycle online multidimensional separation of 10 microg tryptic digest of yeast proteins using nanoflow liquid chromatography coupled with tandem mass spectrometry, and it took 12 h for whole separation under the operating pressure only approximately 900 psi. Totally, 780 distinct proteins were positively identified through assignment of 2953 unique peptides at false-positive rate less than 1%. The good separation performance of this biphasic column was largely attributed to the good orthogonality of the strong-cation exchange monolith and reversed-phase monolith for multidimensional separation.
journal_name
J Proteome Resjournal_title
Journal of proteome researchauthors
Wang F,Dong J,Ye M,Jiang X,Wu R,Zou Hdoi
10.1021/pr700562bsubject
Has Abstractpub_date
2008-01-01 00:00:00pages
306-10issue
1eissn
1535-3893issn
1535-3907journal_volume
7pub_type
杂志文章abstract：:Many neurodegenerative diseases, such as Parkinson's disease, can be directly correlated with the deregulation in neuronal signaling. Hence, it is indispensable for therapy development to understand the participating signaling processes. Because the activity of the involved protein kinases is of major interest for the...
journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr500163r
更新日期：2014-08-01 00:00:00
abstract：:Dehydroeffusol (DHE) is a phenanthrene isolated from the Chinese medicinal plant Juncus effusus. Biological evaluation of DHE reveals in vitro and in vivo anticancer effects. We performed a shotgun proteomic analysis using liquid chromatography-tandem mass spectrometry to investigate the changes in the protein profile...
journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/acs.jproteome.8b00227
更新日期：2018-07-06 00:00:00
abstract：:The use of combinatorial peptide ligand libraries, containing hexapeptides terminating with a primary amine, or modified with a terminal carboxyl group, allowed discovering and identifying a large number of previously unreported proteins in cow's whey. Whereas comprehensive whey protein lists progressively increased i...
journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr900221x
更新日期：2009-08-01 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr900478h
更新日期：2009-11-01 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
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更新日期：2014-06-06 00:00:00
abstract：:Proteomics methods are being increasingly used to study archaeological and palaeontological bone, assisting in species identification and phylogenetic studies as well as improving our understanding of bone diagenesis. More recently, there are developing interests in the study of post-translational modifications, some ...
journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/acs.jproteome.6b00564
更新日期：2017-02-03 00:00:00
abstract：:Tomato fruit and seed allergens were detected by IgE-immunoblotting using sera from 18 adult tomato-sensitized patients selected based on a positive history skin prick test (SPT) and specific Immunglobulin (Ig) E-levels. Isolated tomato seed total protein showed high SPT activity comparable or even higher than tomato ...
journal_title：Journal of proteome research
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更新日期：2009-03-01 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr301067r
更新日期：2013-05-03 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr800507x
更新日期：2009-01-01 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr060525b
更新日期：2007-05-01 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/acs.jproteome.7b00516
更新日期：2017-11-03 00:00:00
abstract：:Glycolysis is the core metabolic pathway supplying energy to cells. Whereas the vast majority of studies focus on specific aspects of the process, global analyses characterizing simultaneously all enzymes involved in the process are scarce. Here, we demonstrate that quantitative label- and standard-free proteomics all...
journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/acs.jproteome.5b00276
更新日期：2015-08-07 00:00:00
abstract：:Enzyme-mediated 18O/16O differential labeling of proteome samples often suffers from incomplete exchange of the carboxy-terminus oxygen atoms, resulting in ambiguity in the measurable abundance differences. In this study, an 18O/16O labeling strategy was optimized for and applied to the solution-based comparative anal...
journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr049790s
更新日期：2005-03-01 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/acs.jproteome.5b01031
更新日期：2016-07-01 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr060586+
更新日期：2007-05-01 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr1011119
更新日期：2011-04-01 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/acs.jproteome.9b00531
更新日期：2020-01-03 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr300273g
更新日期：2012-11-02 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr401056c
更新日期：2014-05-02 00:00:00
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journal_title：Journal of proteome research
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doi：10.1021/pr070392+
更新日期：2008-03-01 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
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更新日期：2009-12-01 00:00:00
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abstract：:Ureteral obstruction will lead clinically to hydronephrosis, which may further develop into partial or complete loss of kidney function and even cause permanent histological damage. However, there is little knowledge of metabolic responses during the obstructed process and its recoverability. In this study, a complete...
journal_title：Journal of proteome research
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更新日期：2018-09-07 00:00:00
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journal_title：Journal of proteome research
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更新日期：2010-07-02 00:00:00
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journal_title：Journal of proteome research
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更新日期：2008-01-01 00:00:00
abstract：:Schwann cells (SCs) are the principal glial cells of the peripheral nervous system (PNS). As a result of tissue heterogeneity and difficulties in the isolation and culture of primary SCs, a considerable understanding of SC biology is obtained from SC lines. However, the differences between the primary SCs and SC lines...
journal_title：Journal of proteome research
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更新日期：2012-06-01 00:00:00
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journal_title：Journal of proteome research
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更新日期：2004-05-01 00:00:00
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journal_title：Journal of proteome research
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更新日期：2014-02-07 00:00:00
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journal_title：Journal of proteome research
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更新日期：2014-02-07 00:00:00
