Abstract：
:Dehydroeffusol (DHE) is a phenanthrene isolated from the Chinese medicinal plant Juncus effusus. Biological evaluation of DHE reveals in vitro and in vivo anticancer effects. We performed a shotgun proteomic analysis using liquid chromatography-tandem mass spectrometry to investigate the changes in the protein profiles in cancer cells upon DHE treatment. DHE affected cancer-associated signaling pathways, including NF-κB, β-catenin, and endoplasmic reticulum stress. Through quantitative pathway and key node analysis of the proteomics data, activating transcription factor 2 (ATF-2) and c-Jun kinase (JNK) were found to be the key components in DHE's modulated biological pathways. Based on the pathway analysis as well as chemical similarity to estradiol, DHE is proposed to be a phytoestrogen. The proteomic, bioinformatic, and chemoinformatic analyses were further verified with individual cell-based experiments. Our study demonstrates a workflow for identifying the mechanisms of action of DHE through shotgun proteomic analysis.
journal_name
J Proteome Resjournal_title
Journal of proteome researchauthors
Chang IS,Sy LK,Cao B,Lum CT,Kwong WL,Fung YE,Lok CN,Che CMdoi
10.1021/acs.jproteome.8b00227subject
Has Abstractpub_date
2018-07-06 00:00:00pages
2470-2479issue
7eissn
1535-3893issn
1535-3907journal_volume
17pub_type
杂志文章abstract：:This report describes the application of NMR spectroscopy to the profiling of metabolites in bronchoalveolar lavage fluid (BALf) of lung transplant recipients without bronchiolitis obliterans syndrome (BOS) (stable, S, n = 10), and with BOS at different degrees of severity (BOS 0p, n = 10; BOS I, n = 10). Through the ...
journal_title：Journal of proteome research
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更新日期：2017-04-07 00:00:00
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journal_title：Journal of proteome research
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journal_title：Journal of proteome research
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journal_title：Journal of proteome research
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journal_title：Journal of proteome research
pub_type： 杂志文章
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journal_title：Journal of proteome research
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更新日期：2010-07-02 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
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更新日期：2008-08-01 00:00:00
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journal_title：Journal of proteome research
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doi：10.1021/pr060648w
更新日期：2007-05-01 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr900918h
更新日期：2010-03-05 00:00:00
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journal_title：Journal of proteome research
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doi：10.1021/pr050364w
更新日期：2006-03-01 00:00:00
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journal_title：Journal of proteome research
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doi：10.1021/pr4004739
更新日期：2013-09-06 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr8009929
更新日期：2009-03-01 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr5004856
更新日期：2014-09-05 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/acs.jproteome.7b00474
更新日期：2017-10-06 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/acs.jproteome.6b00779
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journal_title：Journal of proteome research
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journal_title：Journal of proteome research
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journal_title：Journal of proteome research
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