Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae.

abstract：:Vascular leakage is a hallmark of severe dengue infection. Although extensive studies have been conducted during the past several decades, the molecular mechanisms underlying vascular leakage in dengue shock syndrome (DSS) remain unclear. We thus performed a proteomics study to characterize responses in human endothel...

journal_title：Journal of proteome research

authors： Kanlaya R,Pattanakitsakul SN,Sinchaikul S,Chen ST,Thongboonkerd V

更新日期：2009-05-01 00:00:00

abstract：:The Biology/Disease-driven (B/D) working groups of the Human Proteome Project are alliances of research groups aimed at developing or improving proteomic tools to support specific biological or disease-related research areas. Here, we describe the activities and progress to date of the B/D working group focused on pro...

journal_title：Journal of proteome research

authors： Boersema PJ,Melnik A,Hazenberg BPC,Rezeli M,Marko-Varga G,Kamiie J,Portelius E,Blennow K,Zubarev RA,Polymenidou M,Picotti P

更新日期：2018-12-07 00:00:00

abstract：:Quantifying peptides based on unique peptide fragment ions avoids the issue of ratio distortion that is commonly observed for reporter ion-based quantification approaches. Herein, we present a collision-induced dissociation-cleavable, isobaric acetyl-isoleucine-proline-glycine (Ac-IPG) tag, which conserves the merits ...

journal_title：Journal of proteome research

authors： Tian X,de Vries MP,Permentier HP,Bischoff R

更新日期：2020-09-04 00:00:00

abstract：:An unusual sulfotyrosine-, phosphoserine-containing motif was mapped on a differentially post-translationally modified 60 residue antimicrobial neuroendocrine peptide called chrombacin. The study was performed by high resolution FT MS using complementary fragmentation techniques. The peptide was analyzed at low levels...

journal_title：Journal of proteome research

authors： Taylor SW,Sun C,Hsieh A,Andon NL,Ghosh SS

更新日期：2008-02-01 00:00:00

abstract：:One of the most thoroughly studied insect species, with respect to locomotion behavior, is the stick insect Carausius morosus. Although detailed information exists on premotor networks controlling walking, surprisingly little is known about neuropeptides, which are certainly involved in motor activity generation and m...

journal_title：Journal of proteome research

authors： Liessem S,Ragionieri L,Neupert S,Büschges A,Predel R

更新日期：2018-06-01 00:00:00

abstract：:Hepatocellular carcinoma (HCC) is a major liver malignancy possessing a high mortality rate and is particularly prevalent in China and Asia. While surgery is the most effective treatment for liver tumor, about 80% of HCC patients are inoperable at presentation and die early due to late diagnosis. For early cancer dete...

journal_title：Journal of proteome research

authors： Sun S,Xu MZ,Poon RT,Day PJ,Luk JM

更新日期：2010-01-01 00:00:00

abstract：:Oxidative stress and inflammation play a role in the physiopathology of insulin resistance, diabetes and cardiovascular disease. A single high-fat, high-carbohydrate (HFHC) meal induces an increase in inflammatory and oxidative stress markers in peripheral blood mononuclear cells (PBMC). Previous studies have shown th...

journal_title：Journal of proteome research

authors： Chaves DFS,Carvalho PC,Brasili E,Rogero MM,Hassimotto NA,Diedrich JK,Moresco JJ,Yates JR 3rd,Lajolo FM

更新日期：2017-11-03 00:00:00

abstract：:Metabolic phenotyping of tissues uses metabolomics and lipidomics to measure the relative polar and nonpolar (lipid) metabolite levels in biological samples. This approach aims to understand disease biochemistry and identify biochemical markers of disease. Sample preparation methods must be reproducible, sensitive (hi...

journal_title：Journal of proteome research

authors： Southam AD,Pursell H,Frigerio G,Jankevics A,Weber RJM,Dunn WB

更新日期：2021-01-01 00:00:00

abstract：:Skeletal muscle has emerged as an important secretory organ that produces so-called myokines, regulating energy metabolism via autocrine, paracrine, and endocrine actions; however, the nature and extent of the muscle secretome has not been fully elucidated. Mass spectrometry (MS)-based proteomics, in principle, allows...

journal_title：Journal of proteome research

authors： Deshmukh AS,Cox J,Jensen LJ,Meissner F,Mann M

更新日期：2015-11-06 00:00:00

abstract：:Secreted proteins play a key role in cell signaling, communication, and migration. We recently described the development of an aggressive variant (T24M) of the bladder cancer cell line T24. Using this cell line model, the objective of our work was the identification of secreted proteins involved in the acquisition of ...

journal_title：Journal of proteome research

authors： Makridakis M,Roubelakis MG,Bitsika V,Dimuccio V,Samiotaki M,Kossida S,Panayotou G,Coleman J,Candiano G,Anagnou NP,Vlahou A

更新日期：2010-06-04 00:00:00

abstract：:Protein microarrays consist of known proteins spotted onto solid substrates and are used to perform highly multivariate assessments of protein-binding interactions. Human protein arrays are routinely applied to pathogen detection, immune response biomarker profiling, and antibody specificity profiling. Here, we descri...

journal_title：Journal of proteome research

authors： Feng F,Ataca ST,Ran M,Wang Y,Breen M,Kepler TB

更新日期：2020-07-02 00:00:00

abstract：:Nasopharyngeal carcinoma (NPC) is one of the most common malignant tumors in Southeast Asia. Unfortunately, most NPC victims have had metastasis when first diagnosed due to its deep location and vague symptoms. To date, discovery of sensitive and specific biomarkers for improving detection of NPC remains a challenge. ...

journal_title：Journal of proteome research

authors： Chang YH,Wu CC,Chang KP,Yu JS,Chang YC,Liao PC

更新日期：2009-12-01 00:00:00

abstract：:Diabetes mellitus is a complex polygenic disease caused by gene-environment interactions with multiple complications, and metabonomic analysis is crucial for pathogenesis, early diagnosis, and timely interventions. Here, we comprehensively analyzed the dynamic metabolic changes in rat urine and plasma, which were indu...

journal_title：Journal of proteome research

authors： Xu W,Wu J,An Y,Xiao C,Hao F,Liu H,Wang Y,Tang H

更新日期：2012-06-01 00:00:00

abstract：:Knowledge of the protein networks interacting with the amyloid precursor protein (APP) in vivo can shed light on the physiological function of APP. To date, most proteins interacting with the APP intracellular domain (AICD) have been identified by Yeast Two Hybrid screens which only detect direct interaction partners....

journal_title：Journal of proteome research

authors： Kohli BM,Pflieger D,Mueller LN,Carbonetti G,Aebersold R,Nitsch RM,Konietzko U

更新日期：2012-08-03 00:00:00

abstract：:We propose a method for finding features in liquid chromatography mass spectrometry data that is based on the isotopic pattern of peaks. Our interactive approach to feature finding is carried out across many samples simultaneously and aligns features concurrently. Our scale-independent approach prioritises potential f...

journal_title：Journal of proteome research

authors： Clifford D,Buckley M,Fung KY,Cosgrove L

更新日期：2006-11-01 00:00:00

abstract：:Interstitial cystitis/painful bladder syndrome (IC) is a chronic syndrome of unknown etiology that presents with bladder pain, urinary frequency, and urgency. The lack of specific biomarkers and a poor understanding of underlying molecular mechanisms present challenges for disease diagnosis and therapy. The goals of t...

journal_title：Journal of proteome research

authors： Wen H,Lee T,You S,Park SH,Song H,Eilber KS,Anger JT,Freeman MR,Park S,Kim J

更新日期：2015-01-02 00:00:00

abstract：:A synthetic coumarin, N,N,N-triethyl-11-(4-methyl-2-oxo-2H-chromen-7-yloxy)-11-oxoundecan-1-aminium bromide (SCD-1), having potent activity against pathogenic Aspergilli (MIC90 15.62 μg/mL), was investigated to identify its molecular targets in the pathogen. The proteome of Aspergillus fumigatus was developed after tr...

journal_title：Journal of proteome research

authors： Singh S,Gupta S,Singh B,Sharma SK,Gupta VK,Sharma GL

更新日期：2012-06-01 00:00:00

abstract：:Ovarian cancer remains a deadly threat to women as the disease is often diagnosed in the late stages when the chance of survival is low. There are no good biomarkers available for early detection and only a few markers have shown clinical utility for prognosis, response to therapy and disease recurrence. We mined cond...

journal_title：Journal of proteome research

authors： Gunawardana CG,Kuk C,Smith CR,Batruch I,Soosaipillai A,Diamandis EP

更新日期：2009-10-01 00:00:00

abstract：:Tryptic digestion of biotinylated Lys-C peptides followed by affinity chromatography allows the selective isolation of lysine-free tryptic peptides delimited by arginine residues (RRnK peptides). In silico analysis revealed that RRnK peptides represent 87% of the whole proteomes and their specific isolation simplifies...

journal_title：Journal of proteome research

authors： Sánchez A,González LJ,Ramos Y,Betancourt L,Gil J,Besada V,Fernández-de-Cossio J,Alvarez F,Padrón G

更新日期：2006-05-01 00:00:00

abstract：:As large-scale cross-linking data becomes available, new software tools for data processing and visualization are required to replace manual data analysis. XLink-DB serves as a data storage site and visualization tool for cross-linking results. XLink-DB accepts data generated with any cross-linker and stores them in a...

journal_title：Journal of proteome research

authors： Zheng C,Weisbrod CR,Chavez JD,Eng JK,Sharma V,Wu X,Bruce JE

更新日期：2013-04-05 00:00:00

abstract：:Quantitative proteomics experiments, using for instance isobaric tandem mass tagging approaches, are conducive to measuring changes in protein abundance over multiple time points in response to one or more conditions or stimulations. The aim is often to determine which proteins exhibit similar patterns within and acro...

journal_title：Journal of proteome research

authors： Lee LH,Halu A,Morgan S,Iwata H,Aikawa M,Singh SA

更新日期：2019-02-01 00:00:00

abstract：:The plasma of patients with stable carotid atherosclerosis (n = 9), and healthy subjects (n = 10) have been fingerprinted with both GC-MS and (1)H NMR. Principal component analysis (PCA), partial least-squares-discriminant analysis (PLS-DA) and orthogonal partial least-squares-discriminant analysis (OPLS-DA) have been...

journal_title：Journal of proteome research

authors： Teul J,Rupérez FJ,Garcia A,Vaysse J,Balayssac S,Gilard V,Malet-Martino M,Martin-Ventura JL,Blanco-Colio LM,Tuñón J,Egido J,Barbas C

更新日期：2009-12-01 00:00:00

abstract：:Ozone (O3), a potent air pollutant and a significant greenhouse gas, has been recognized as a major component of global climate change. However, current increasing trends in its background level are projecting a more severe threat to natural and cultivated plants in the near future. The present study has been designed...

journal_title：Journal of proteome research

authors： Sarkar A,Rakwal R,Bhushan Agrawal S,Shibato J,Ogawa Y,Yoshida Y,Kumar Agrawal G,Agrawal M

更新日期：2010-09-03 00:00:00

abstract：:Defects in the gene encoding the persulfide dioxygenase ETHE1 are known to cause the severe inherited metabolic disorder ethylmalonic encephalopathy (EE). In spite of known clinical characteristics, the molecular mechanisms underlying the ETHE1 deficiency are still obscure. Herein, to further analyze the molecular phe...

journal_title：Journal of proteome research

authors： Sahebekhtiari N,Nielsen CB,Johannsen M,Palmfeldt J

更新日期：2016-05-06 00:00:00

abstract：:We report here using a novel technology-electrospray-assisted laser desorption ionization (ELDI)/mass spectrometry-for the rapid and sensitive detection of the major proteins that exist in dried biological fluids (e.g., blood, tears, saliva, serum), bacterial cultures, and tissues (e.g., porcine liver and heart) under...

journal_title：Journal of proteome research

authors： Huang MZ,Hsu HJ,Lee JY,Jeng J,Shiea J

更新日期：2006-05-01 00:00:00

abstract：:Many proteins can be modified by multiple types of post-translational modifications (Mtp-proteins). Although some post-translational modifications (PTMs) have recently been found to be associated with life-threatening diseases like cancers and neurodegenerative disorders, the underlying mechanisms remain enigmatic to ...

journal_title：Journal of proteome research

authors： Huang Q,Chang J,Cheung MK,Nong W,Li L,Lee MT,Kwan HS

更新日期：2014-06-06 00:00:00

abstract：:Cardiovascular disease is the leading cause of diabetic morbidity with more than 10% of type 1 diabetes mellitus (T1DM) patients dying before they are 40 years old. This study utilized Akita mice, a murine model with T1DM progression analogous to that of humans. Diabetic cardiomyopathy in Akita mice presents as cardia...

journal_title：Journal of proteome research

authors： Dewey S,Lai X,Witzmann FA,Sohal M,Gomes AV

更新日期：2013-09-06 00:00:00

abstract：:Phospholipase C gamma 1 (PLC-γ1) occupies a critically important position in the T-cell signaling pathway. While its functions as a regulator of both Ca2+ signaling and PKC-family kinases are well characterized, PLC-γ1's role in the regulation of early T-cell receptor signaling events is incompletely understood. Activ...

journal_title：Journal of proteome research

authors： Belmont J,Gu T,Mudd A,Salomon AR

更新日期：2017-08-04 00:00:00

abstract：:The past decade has been marked by the emergence of selective affinity media and sensitive mass spectrometry instrumentation that facilitated large-scale phosphoproteome analyses and expanded the repertoire of protein phosphorylation. Despite these remarkable advances, the precise location of the phosphorylation site ...

journal_title：Journal of proteome research

authors： Courcelles M,Bridon G,Lemieux S,Thibault P

更新日期：2012-07-06 00:00:00

abstract：:Reliable statistical validation of peptide and protein identifications is a top priority in large-scale mass spectrometry based proteomics. PeptideProphet is one of the computational tools commonly used for assessing the statistical confidence in peptide assignments to tandem mass spectra obtained using database searc...

journal_title：Journal of proteome research

authors： Choi H,Ghosh D,Nesvizhskii AI

更新日期：2008-01-01 00:00:00