A sulfated, phosphorylated 7 kDa secreted peptide characterized by direct analysis of cell culture media.

Abstract:

:An unusual sulfotyrosine-, phosphoserine-containing motif was mapped on a differentially post-translationally modified 60 residue antimicrobial neuroendocrine peptide called chrombacin. The study was performed by high resolution FT MS using complementary fragmentation techniques. The peptide was analyzed at low levels directly from cell culture media in contrast to previous reports that required extensive purification and proteolytic digestion. The sulfation site was not previously described nor predicted by informatic analysis of the peptide's precursor sequence.

journal_name

J Proteome Res

authors

Taylor SW,Sun C,Hsieh A,Andon NL,Ghosh SS

doi

10.1021/pr7006686

subject

Has Abstract

pub_date

2008-02-01 00:00:00

pages

795-802

issue

2

eissn

1535-3893

issn

1535-3907

journal_volume

7

pub_type

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