A sulfated, phosphorylated 7 kDa secreted peptide characterized by direct analysis of cell culture media.


:An unusual sulfotyrosine-, phosphoserine-containing motif was mapped on a differentially post-translationally modified 60 residue antimicrobial neuroendocrine peptide called chrombacin. The study was performed by high resolution FT MS using complementary fragmentation techniques. The peptide was analyzed at low levels directly from cell culture media in contrast to previous reports that required extensive purification and proteolytic digestion. The sulfation site was not previously described nor predicted by informatic analysis of the peptide's precursor sequence.


J Proteome Res


Taylor SW,Sun C,Hsieh A,Andon NL,Ghosh SS




Has Abstract


2008-02-01 00:00:00












  • Thioridazine Alters the Cell-Envelope Permeability of Mycobacterium tuberculosis.

    abstract::The increasing occurrence of multidrug resistant tuberculosis exerts a major burden on treatment of this infectious disease. Thioridazine, previously used as a neuroleptic, is active against extensively drug resistant tuberculosis when added to other second- and third-line antibiotics. By quantitatively studying the p...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: de Keijzer J,Mulder A,de Haas PE,de Ru AH,Heerkens EM,Amaral L,van Soolingen D,van Veelen PA

    更新日期:2016-06-03 00:00:00

  • Global Metabonomic and Proteomic Analysis of Human Conjunctival Epithelial Cells (IOBA-NHC) in Response to Hyperosmotic Stress.

    abstract::"Dry eye" is a multifactorial inflammatory disease affecting the ocular surface. Tear hyperosmolarity in dry eye contributes to inflammation and cell damage. Recent research efforts on dry eye have been directed toward biomarker discovery for diagnosis, response to treatment, and disease mechanisms. This study employe...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Chen L,Li J,Guo T,Ghosh S,Koh SK,Tian D,Zhang L,Jia D,Beuerman RW,Aebersold R,Chan EC,Zhou L

    更新日期:2015-09-04 00:00:00

  • Optimized fast and sensitive acquisition methods for shotgun proteomics on a quadrupole orbitrap mass spectrometer.

    abstract::Advances in proteomics are continually driven by the introduction of new mass spectrometric instrumentation with improved performances. The recently introduced quadrupole Orbitrap (Q Exactive) tandem mass spectrometer allows fast acquisition of high-resolution higher-energy collisional dissociation (HCD) tandem mass s...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Kelstrup CD,Young C,Lavallee R,Nielsen ML,Olsen JV

    更新日期:2012-06-01 00:00:00

  • Poly(glycidyl methacrylate/divinylbenzene)-IDA-FeIII in phosphoproteomics.

    abstract::The study of protein phosphorylation has grown exponentially in recent years, as it became evident that important cellular functions are regulated by phosphorylation and dephosphorylation of proteins on serine, threonine and tyrosine residues. The use of immobilized metal affinity chromatography (IMAC) to enrich phosp...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Aprilita NH,Huck CW,Bakry R,Feuerstein I,Stecher G,Morandell S,Huang HL,Stasyk T,Huber LA,Bonn GK

    更新日期:2005-11-01 00:00:00

  • Proteome changes after metabolic engineering to enhance aerobic mineralization of cis-1,2-dichloroethylene.

    abstract::Metabolically engineered Escherichia coli has previously been used to degrade cis-1,2-dichloroethylene (cis-DCE). The strains express the six genes of an evolved toluene ortho-monooxygenase from Burkholderia cepacia G4 (TOM-Green, which formed a reactive epoxide) with either (1) gamma-glutamylcysteine synthetase (GSHI...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Lee J,Cao L,Ow SY,Barrios-Llerena ME,Chen W,Wood TK,Wright PC

    更新日期:2006-06-01 00:00:00

  • Regulated glycosylation patterns of IgG during alloimmune responses against human platelet antigens.

    abstract::Various biological activities of immunoglobulin G (IgG) including antibody-dependent cellular cytotoxicity (ADCC) are modulated by the structural features of the N-glycans in the Fc part. In this study, we describe a population of IgG1 alloantibodies which are formed during pregnancy against human platelet antigens (H...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Wuhrer M,Porcelijn L,Kapur R,Koeleman CA,Deelder A,de Haas M,Vidarsson G

    更新日期:2009-02-01 00:00:00

  • Comparative Glycoproteomic Profiling of Human Body Fluid between Healthy Controls and Patients with Papillary Thyroid Carcinoma.

    abstract::Papillary thyroid carcinoma (PTC) is the most common type of thyroid cancer among women worldwide. It is confirmed mainly by fine-needle aspiration biopsy (FNAB), an invasive diagnostic method. The key proteins responsible for thyroid hormone biosynthesis are glycosylated. Hence, changes in site-specific glycosylation...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Zhang Y,Zhao W,Zhao Y,Mao Y,Su T,Zhong Y,Wang S,Zhai R,Cheng J,Fang X,Zhu J,Yang H

    更新日期:2020-07-02 00:00:00

  • Site-Specific N-Glycosylation Characterization of Windmill Palm Tree Peroxidase Using Novel Tools for Analysis of Plant Glycopeptide Mass Spectrometry Data.

    abstract::Plant secretory (Class III) peroxidases are redox enzymes that rely on N-glycosylation for full enzyme activity and stability. Peroxidases from palm tree leaves comprise the most stable and active plant peroxidases characterized to date. Herein, site-specific glycosylation and microheterogeneity of windmill palm tree ...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Baker MR,Tabb DL,Ching T,Zimmerman LJ,Sakharov IY,Li QX

    更新日期:2016-06-03 00:00:00

  • Human tissue profiling with multidimensional protein identification technology.

    abstract::Profiling of tissues and cell types through systematic characterization of expressed genes or proteins shows promise as a basic research tool, and has potential applications in disease diagnosis and classification. We used multidimensional protein identification protein identification technology (MudPIT) to analyze pr...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Cagney G,Park S,Chung C,Tong B,O'Dushlaine C,Shields DC,Emili A

    更新日期:2005-09-01 00:00:00

  • Comprehensive appraisal of the extracellular proteins from a monoderm bacterium: theoretical and empirical exoproteomes of Listeria monocytogenes EGD-e by secretomics.

    abstract::Defined as proteins actively transported via secretion systems, secreted proteins can have radically different subcellular destinations in monoderm (Gram-positive) bacteria. From degradative enzymes in saprophytes to virulence factors in pathogens, secreted proteins are the main tools used by bacteria to interact with...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Desvaux M,Dumas E,Chafsey I,Chambon C,Hébraud M

    更新日期:2010-10-01 00:00:00

  • Serum metabolic signatures of fulminant type 1 diabetes.

    abstract::Fulminant type 1 diabetes (FT1DM) is a relatively new clinical entity featured by acute destruction of pancreatic beta cells. Clinical consequences of FT1DM could be fatal when timely medications are not provided, suggesting the particular importance of rapid and accurate diagnosis. Here we report a serum metabonomics...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Lu J,Zhou J,Bao Y,Chen T,Zhang Y,Zhao A,Qiu Y,Xie G,Wang C,Jia W,Jia W

    更新日期:2012-09-07 00:00:00

  • Gain-Scanning for Protein Microarray Assays.

    abstract::Protein microarrays consist of known proteins spotted onto solid substrates and are used to perform highly multivariate assessments of protein-binding interactions. Human protein arrays are routinely applied to pathogen detection, immune response biomarker profiling, and antibody specificity profiling. Here, we descri...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Feng F,Ataca ST,Ran M,Wang Y,Breen M,Kepler TB

    更新日期:2020-07-02 00:00:00

  • Streptozotocin-induced dynamic metabonomic changes in rat biofluids.

    abstract::Diabetes mellitus is a complex polygenic disease caused by gene-environment interactions with multiple complications, and metabonomic analysis is crucial for pathogenesis, early diagnosis, and timely interventions. Here, we comprehensively analyzed the dynamic metabolic changes in rat urine and plasma, which were indu...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Xu W,Wu J,An Y,Xiao C,Hao F,Liu H,Wang Y,Tang H

    更新日期:2012-06-01 00:00:00

  • Liquid- and gas-phase nitration of bovine serum albumin studied by LC-MS and LC-MS/MS using monolithic columns.

    abstract::Post-translational nitration of proteins was analyzed by capillary reversed-phase high-performance liquid chromatography (RP-HPLC) on-line interfaced to electrospray ionization mass spectrometry (ESI--MS) or tandem mass spectrometry (ESI--MS/MS). Both methods were compared using a tryptic digest of bovine serum albumi...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Walcher W,Franze T,Weller MG,Pöschl U,Huber CG

    更新日期:2003-09-01 00:00:00

  • Comprehensive proteomic mass spectrometric characterization of human cannabinoid CB2 receptor.

    abstract::The CB1 and CB2 cannabinoid receptors belong to the GPCR superfamily and are associated with a variety of physiological and pathophysiological processes. Both receptors, with several lead compounds at different phases of development, are potentially useful targets for drug discovery. For this reason, fully elucidating...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Zvonok N,Yaddanapudi S,Williams J,Dai S,Dong K,Rejtar T,Karger BL,Makriyannis A

    更新日期:2007-06-01 00:00:00

  • Metabolic regulatory network alterations in response to acute cold stress and ginsenoside intervention.

    abstract::Acute stress may trigger systemic biochemical and physiological changes in living organisms, leading to a rapid loss of homeostasis, which can be gradually reinstated by self-regulatory mechanisms and/or drug intervention strategy. However, such a sophisticated metabolic regulatory process has so far been poorly under...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Wang X,Su M,Qiu Y,Ni Y,Zhao T,Zhou M,Zhao A,Yang S,Zhao L,Jia W

    更新日期:2007-09-01 00:00:00

  • Evaluation of protein depletion methods for the analysis of total-, phospho- and glycoproteins in lumbar cerebrospinal fluid.

    abstract::A proper sample preparation, in particular, abundant protein removal is crucial in the characterization of low-abundance proteins including those harboring post-translational modifications. In human cerebrospinal fluid (CSF), approximately 80% of proteins originate from serum, and removal of major proteins is necessar...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Ogata Y,Charlesworth MC,Muddiman DC

    更新日期:2005-05-01 00:00:00

  • Mapping post-translational modifications of mammalian testicular specific histone variant TH2B in tetraploid and haploid germ cells and their implications on the dynamics of nucleosome structure.

    abstract::Histones regulate a variety of chromatin templated events by their post-translational modifications (PTMs). Although there are extensive reports on the PTMs of canonical histones, the information on the histone variants remains very scanty. Here, we report the identification of different PTMs, such as acetylation, met...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Pentakota SK,Sandhya S,P Sikarwar A,Chandra N,Satyanarayana Rao MR

    更新日期:2014-12-05 00:00:00

  • Optimization of Search Engines and Postprocessing Approaches to Maximize Peptide and Protein Identification for High-Resolution Mass Data.

    abstract::The two key steps for analyzing proteomic data generated by high-resolution MS are database searching and postprocessing. While the two steps are interrelated, studies on their combinatory effects and the optimization of these procedures have not been adequately conducted. Here, we investigated the performance of thre...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Tu C,Sheng Q,Li J,Ma D,Shen X,Wang X,Shyr Y,Yi Z,Qu J

    更新日期:2015-11-06 00:00:00

  • Alterations in actin cytoskeletal assembly and junctional protein complexes in human endothelial cells induced by dengue virus infection and mimicry of leukocyte transendothelial migration.

    abstract::Vascular leakage is a hallmark of severe dengue infection. Although extensive studies have been conducted during the past several decades, the molecular mechanisms underlying vascular leakage in dengue shock syndrome (DSS) remain unclear. We thus performed a proteomics study to characterize responses in human endothel...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Kanlaya R,Pattanakitsakul SN,Sinchaikul S,Chen ST,Thongboonkerd V

    更新日期:2009-05-01 00:00:00

  • Porphyromonas gingivalis Gingipains Display Transpeptidation Activity.

    abstract::Porphyromonas gingivalis is a keystone periodontal pathogen that has been associated with autoimmune disorders. The cell surface proteases Lys-gingipain (Kgp) and Arg-gingipains (RgpA and RgpB) are major virulence factors, and their proteolytic activity is enhanced by small peptides such as glycylglycine (GlyGly). The...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Zhang L,Veith PD,Huq NL,Chen YY,Seers CA,Cross KJ,Gorasia DG,Reynolds EC

    更新日期:2018-08-03 00:00:00

  • Proteomic analysis of butyrate effects and loss of butyrate sensitivity in HT29 colorectal cancer cells.

    abstract::Butyrate, a fermentation product of the large bowel microflora, is potentially protective against the development of colorectal cancer. In vitro, butyrate has been shown to induce apoptosis and inhibit proliferation in numerous cancer cell lines, including colorectal cancer. Although these tumor suppressing properties...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Fung KY,Lewanowitsch T,Henderson ST,Priebe I,Hoffmann P,McColl SR,Lockett T,Head R,Cosgrove LJ

    更新日期:2009-03-01 00:00:00

  • Overexpression of CD38 decreases cellular NAD levels and alters the expression of proteins involved in energy metabolism and antioxidant defense.

    abstract::Nicotinamide adenine dinucleotide (NAD) is a coenzyme found in all living cells and mediates multiple cellular signaling pathways. In the present study, a 35% decrease of cellular NAD level is achieved by stable expression of the N-terminal truncated CD38, a NAD hydrolase. CD38-expressing (CD38(+)) cells have the lowe...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Hu Y,Wang H,Wang Q,Deng H

    更新日期:2014-02-07 00:00:00

  • Lipid Profiling in Epicardial and Subcutaneous Adipose Tissue of Patients with Coronary Artery Disease.

    abstract::Coronary artery disease is one of the most frequent causes of morbidity and mortality worldwide. It is even more prevalent in patients with type 2 diabetes mellitus who suffer from obesity and increased accumulation of epicardial fat with a possible contributing role in the development of coronary artery disease. We p...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Tomášová P,Čermáková M,Pelantová H,Vecka M,Kratochvílová H,Lipš M,Lindner J,Ivák P,Netuka I,Šedivá B,Haluzík M,Kuzma M

    更新日期:2020-10-02 00:00:00

  • Quantitative proteome analyses identify PrfA-responsive proteins and phosphoproteins in Listeria monocytogenes.

    abstract::Protein phosphorylation is a major mechanism of signal transduction in bacteria. Here, we analyzed the proteome and phosphoproteome of a wild-type strain of the food-borne pathogen Listeria monocytogenes that was grown in either chemically defined medium or rich medium containing glucose. We then compared these result...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Misra SK,Moussan Désirée Aké F,Wu Z,Milohanic E,Cao TN,Cossart P,Deutscher J,Monnet V,Archambaud C,Henry C

    更新日期:2014-12-05 00:00:00

  • Proteomic analysis of lipid microdomains from lipopolysaccharide-activated human endothelial cells.

    abstract::The endothelium plays a critical role in orchestrating the inflammatory response seen during sepsis. Many of the inflammatory effects of Gram-negative sepsis are elicited by lipopolysaccharide (LPS), a glycolipid component of bacterial cell walls. Lipid-rich microdomains have been shown to concentrate components of th...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Karsan A,Blonder J,Law J,Yaquian E,Lucas DA,Conrads TP,Veenstra T

    更新日期:2005-03-01 00:00:00

  • Early events in plastid protein degradation in stay-green Arabidopsis reveal differential regulation beyond the retention of LHCII and chlorophyll.

    abstract::An individually darkened leaf model was used to study protein changes in the Arabidopsis mutant stay-green1 (sgr1) to partially mimic the process of leaf covering senescence that occurs naturally in the shaded rosettes of Arabidopsis plants. Utilizing this controlled and predictable induced senescence model has allowe...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Grassl J,Pružinská A,Hörtensteiner S,Taylor NL,Millar AH

    更新日期:2012-11-02 00:00:00

  • Integrative analysis of N-linked human glycoproteomic data sets reveals PTPRF ectodomain as a novel plasma biomarker candidate for prostate cancer.

    abstract::In an attempt to identify prostate cancer biomarkers with greater diagnostic and prognostic capabilities, we have developed an integrative proteomic discovery workflow focused on N-linked glycoproteins that refines the target selection process. In this work, hydrazide-based chemistry was used to identify N-linked glyc...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Whitmore TE,Peterson A,Holzman T,Eastham A,Amon L,McIntosh M,Ozinsky A,Nelson PS,Martin DB

    更新日期:2012-05-04 00:00:00

  • GC-MS-Based Metabolome and Metabolite Regulation in Serum-Resistant Streptococcus agalactiae.

    abstract::Streptococcus agalactiae causes severe systemic infections in human and fish. In the present study, we established a pathogen-plasma interaction model by which we explored how S. agalactiae evaded serum-mediated killing. We found that S. agalactiae grew faster in the presence of yellow grouper plasma than in the absen...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Wang Z,Li MY,Peng B,Cheng ZX,Li H,Peng XX

    更新日期:2016-07-01 00:00:00

  • Identification of novel accessible proteins bearing diagnostic and therapeutic potential in human pancreatic ductal adenocarcinoma.

    abstract::Pancreas ductal adenocarcinoma (PDAC) remains a deadly malignancy with poor early diagnostic and no effective therapy. Although several proteomic studies have performed comparative analysis between normal and malignant tissues, there is a lack of clear characterization of proteins that could be of clinical value. Syst...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Turtoi A,Musmeci D,Wang Y,Dumont B,Somja J,Bevilacqua G,De Pauw E,Delvenne P,Castronovo V

    更新日期:2011-09-02 00:00:00