A sulfated, phosphorylated 7 kDa secreted peptide characterized by direct analysis of cell culture media.


:An unusual sulfotyrosine-, phosphoserine-containing motif was mapped on a differentially post-translationally modified 60 residue antimicrobial neuroendocrine peptide called chrombacin. The study was performed by high resolution FT MS using complementary fragmentation techniques. The peptide was analyzed at low levels directly from cell culture media in contrast to previous reports that required extensive purification and proteolytic digestion. The sulfation site was not previously described nor predicted by informatic analysis of the peptide's precursor sequence.


J Proteome Res


Taylor SW,Sun C,Hsieh A,Andon NL,Ghosh SS




Has Abstract


2008-02-01 00:00:00












  • Proteomic characterization of human milk whey proteins during a twelve-month lactation period.

    abstract::Human milk is a rich source of bioactive proteins that support the early growth and development of the newborn. Although the major components of the protein fraction in human milk have been studied, the expression and relative abundance of minor components have received limited attention. We examined the expression of...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Liao Y,Alvarado R,Phinney B,Lönnerdal B

    更新日期:2011-04-01 00:00:00

  • Alterations in actin cytoskeletal assembly and junctional protein complexes in human endothelial cells induced by dengue virus infection and mimicry of leukocyte transendothelial migration.

    abstract::Vascular leakage is a hallmark of severe dengue infection. Although extensive studies have been conducted during the past several decades, the molecular mechanisms underlying vascular leakage in dengue shock syndrome (DSS) remain unclear. We thus performed a proteomics study to characterize responses in human endothel...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Kanlaya R,Pattanakitsakul SN,Sinchaikul S,Chen ST,Thongboonkerd V

    更新日期:2009-05-01 00:00:00

  • Stage-specific proteome signatures in early bovine embryo development.

    abstract::Development of early embryonic stages before activation of the embryonic genome depends on sufficiently stored products of the maternal genome, adequate recruitment and degradation of mRNAs, as well as activation, deactivation, and relocation of proteins. By application of an isobaric tagging for relative and absolute...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Deutsch DR,Fröhlich T,Otte KA,Beck A,Habermann FA,Wolf E,Arnold GJ

    更新日期:2014-10-03 00:00:00

  • Discovery of Metabolite Biomarkers for Acute Ischemic Stroke Progression.

    abstract::Stroke remains a major public health problem worldwide; it causes severe disability and is associated with high mortality rates. However, early diagnosis of stroke is difficult, and no reliable biomarkers are currently established. In this study, mass-spectrometry-based metabolomics was utilized to characterize the me...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Liu P,Li R,Antonov AA,Wang L,Li W,Hua Y,Guo H,Wang L,Liu P,Chen L,Tian Y,Xu F,Zhang Z,Zhu Y,Huang Y

    更新日期:2017-02-03 00:00:00

  • Processing of data generated by 2-dimensional gel electrophoresis for statistical analysis: missing data, normalization, and statistics.

    abstract::Several high-throughput statistical methods were evaluated for processing data generated by two-dimensional polyacrylamide gel electrophoresis, including how to handle missing data, normalization, and statistical analysis of data obtained from 2-D gels. Quantile normalization combined with a nonparametric permutation ...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Chang J,Van Remmen H,Ward WF,Regnier FE,Richardson A,Cornell J

    更新日期:2004-11-01 00:00:00

  • Development of an annotated library of neutral human milk oligosaccharides.

    abstract::Human milk oligosaccharides (HMOs) perform a number of functions including serving as prebiotics to stimulate the growth of beneficial intestinal bacteria, as receptor analogues to inhibit binding of pathogens, and as substances that promote postnatal brain development. There is further evidence that HMOs participate ...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Wu S,Tao N,German JB,Grimm R,Lebrilla CB

    更新日期:2010-08-06 00:00:00

  • Proteomic analysis of near-isogenic sunflower varieties differing in seed oil traits.

    abstract::Near-isogenic sunflower lines containing 25% (inbred RHA280) and 48% (RHA801) oil by seed dry mass were comparatively analyzed in biological triplicate at 18 days after flowering using two-dimensional (both pI 3-10 and 4-7) Difference Gel Electrophoresis. Additionally, two inbred lines varying in oleic acid content, H...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Hajduch M,Casteel JE,Tang S,Hearne LB,Knapp S,Thelen JJ

    更新日期:2007-08-01 00:00:00

  • Combined transcriptomic-(1)H NMR metabonomic study reveals that monoethylhexyl phthalate stimulates adipogenesis and glyceroneogenesis in human adipocytes.

    abstract::Adipose tissue is a major storage site for lipophilic environmental contaminants. The environmental metabolic disruptor hypothesis postulates that some pollutants can promote obesity or metabolic disorders by activating nuclear receptors involved in the control of energetic homeostasis. In this context, monoethylhexyl...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Ellero-Simatos S,Claus SP,Benelli C,Forest C,Letourneur F,Cagnard N,Beaune PH,de Waziers I

    更新日期:2011-12-02 00:00:00

  • Proteomic approach to identify champagne wine proteins as modified by Botrytis cinerea infection.

    abstract::The presence of the fungal pathogen, Botrytis cinerea, in the vineyard causes reductions in both quality and quantity of grapes and wine. Because proteins are involved in the foam stabilization of sparkling wines, we have undertaken, for the first time, a thorough proteomic analysis of two champagne base wines prepare...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Cilindre C,Jégou S,Hovasse A,Schaeffer C,Castro AJ,Clément C,Van Dorsselaer A,Jeandet P,Marchal R

    更新日期:2008-03-01 00:00:00

  • Direct protein detection from biological media through electrospray-assisted laser desorption ionization/mass spectrometry.

    abstract::We report here using a novel technology-electrospray-assisted laser desorption ionization (ELDI)/mass spectrometry-for the rapid and sensitive detection of the major proteins that exist in dried biological fluids (e.g., blood, tears, saliva, serum), bacterial cultures, and tissues (e.g., porcine liver and heart) under...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Huang MZ,Hsu HJ,Lee JY,Jeng J,Shiea J

    更新日期:2006-05-01 00:00:00

  • Serum Antibody Profile during Colonization of the Mouse Gut by Candida albicans: Relevance for Protection during Systemic Infection.

    abstract::Candida albicans is a commensal microorganism in the oral cavity and gastrointestinal and urogenital tracts of most individuals that acts as an opportunistic pathogen when the host immune response is reduced. Here, we established different immunocompetent murine models to analyze the antibody responses to the C. albic...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Huertas B,Prieto D,Pitarch A,Gil C,Pla J,Díez-Orejas R

    更新日期:2017-01-06 00:00:00

  • NMRmix: A Tool for the Optimization of Compound Mixtures in 1D (1)H NMR Ligand Affinity Screens.

    abstract::NMR ligand affinity screening is a powerful technique that is routinely used in drug discovery or functional genomics to directly detect protein-ligand binding events. Binding events can be identified by monitoring differences in the 1D (1)H NMR spectrum of a compound with and without protein. Although a single NMR sp...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Stark JL,Eghbalnia HR,Lee W,Westler WM,Markley JL

    更新日期:2016-04-01 00:00:00

  • Liquid chromatography-mass spectrometry based metabolomics study of cloned versus normal pigs fed either restricted or ad libitum high-energy diets.

    abstract::Genetically identical cloned pigs should in principle eliminate biological variation and provide more pronounced effects when subjected to, e.g., dietary interventions, but little is known about how phenotype and phenotypic variation is affected by cloning. Therefore, an investigation of the metabolome of cloned pigs ...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Christensen KL,Hedemann MS,Jørgensen H,Stagsted J,Knudsen KE

    更新日期:2012-07-06 00:00:00

  • Identification of the amyloid β-protein precursor and cystatin C as novel epidermal growth factor receptor regulated secretory proteins in nasopharyngeal carcinoma by proteomics.

    abstract::The epidermal growth factor receptor (EGFR) is usually overexpressed in nasopharyngeal carcinoma (NPC) and is associated with pathogenesis of NPC. However, while EGFR-modulated intracellular proteins have been extensively studied, little is known concerning their extracellular counterparts. To identify EGFR-regulated ...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Tang CE,Guan YJ,Yi B,Li XH,Liang K,Zou HY,Yi H,Li MY,Zhang PF,Li C,Peng F,Chen ZC,Yao KT,Xiao ZQ

    更新日期:2010-12-03 00:00:00

  • Temperature Dependence of the Proteome Profile of the Psychrotolerant Pathogenic Food Spoiler Bacillus weihenstephanensis Type Strain WSBC 10204.

    abstract::Bacillus weihenstephanensis is a subspecies of the Bacillus cereus sensu lato group of spore-forming bacteria known to cause food spoilage or food poisoning. The key distinguishing phenotype of B. weihenstephanensis is its ability to grow below 7 °C or, from a food safety perspective, to grow and potentially produce t...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Stelder SK,Mahmud SA,Dekker HL,de Koning LJ,Brul S,de Koster CG

    更新日期:2015-05-01 00:00:00

  • A data-mining scheme for identifying peptide structural motifs responsible for different MS/MS fragmentation intensity patterns.

    abstract::Although tandem mass spectrometry (MS/MS) has become an integral part of proteomics, intensity patterns in MS/MS spectra are rarely weighted heavily in most widely used algorithms because they are not yet fully understood. Here a knowledge mining approach is demonstrated to discover fragmentation intensity patterns an...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Huang Y,Tseng GC,Yuan S,Pasa-Tolic L,Lipton MS,Smith RD,Wysocki VH

    更新日期:2008-01-01 00:00:00

  • Expansion for the Brachylophosaurus canadensis Collagen I Sequence and Additional Evidence of the Preservation of Cretaceous Protein.

    abstract::Sequence data from biomolecules such as DNA and proteins, which provide critical information for evolutionary studies, have been assumed to be forever outside the reach of dinosaur paleontology. Proteins, which are predicted to have greater longevity than DNA, have been recovered from two nonavian dinosaurs, but these...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Schroeter ER,DeHart CJ,Cleland TP,Zheng W,Thomas PM,Kelleher NL,Bern M,Schweitzer MH

    更新日期:2017-02-03 00:00:00

  • Proteomic comparison between maturation drying and prematurely imposed drying of Zea mays seeds reveals a potential role of maturation drying in preparing proteins for seed germination, seedling vigor, and pathogen resistance.

    abstract::We have studied the role(s) of maturation drying in the acquisition of germinability, seedling vigor and pathogen resistance by comparing the proteome changes in maize embryo and endosperm during mature and prematurely imposed drying. Prematurely imposed dried seeds at 40 days after pollination (DAP) germinated almost...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Wang WQ,Ye JQ,Rogowska-Wrzesinska A,Wojdyla KI,Jensen ON,Møller IM,Song SQ

    更新日期:2014-02-07 00:00:00

  • Differentiating proteomic biomarkers in breast cancer by laser capture microdissection and MALDI MS.

    abstract::We assessed proteomic patterns in breast cancer using MALDI MS and laser capture microdissected cells. Protein and peptide expression in invasive mammary carcinoma versus normal mammary epithelium and estrogen-receptor positive versus estrogen-receptor negative tumors were compared. Biomarker candidates were identifie...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Sanders ME,Dias EC,Xu BJ,Mobley JA,Billheimer D,Roder H,Grigorieva J,Dowsett M,Arteaga CL,Caprioli RM

    更新日期:2008-04-01 00:00:00

  • Proteomic analysis of leaves and roots of common wheat (Triticum aestivum L.) under copper-stress conditions.

    abstract::Proteomic studies were performed to identify the protein species involved in copper (Cu) stress responses in common wheat. Two-week-old wheat seedlings were exposed to 100 μM CuSO4 treatment for 3 days. Growth of shoots and roots was markedly inhibited and lipid peroxidation was greatly increased. Cu was readily absor...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Li G,Peng X,Xuan H,Wei L,Yang Y,Guo T,Kang G

    更新日期:2013-11-01 00:00:00

  • Radioactive Chernobyl environment has produced high-oil flax seeds that show proteome alterations related to carbon metabolism during seed development.

    abstract::Starting in 2007, we have grown soybean (Glycine max [L.] Merr. variety Soniachna) and flax (Linum usitatissimum, L. variety Kyivskyi) in the radio-contaminated Chernobyl area and analyzed the seed proteomes. In the second-generation flax seeds, we detected a 12% increase in oil content. To characterize the bases for ...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Klubicová K,Danchenko M,Skultety L,Berezhna VV,Rashydov NM,Hajduch M

    更新日期:2013-11-01 00:00:00

  • Improving the identification rate of endogenous peptides using electron transfer dissociation and collision-induced dissociation.

    abstract::Tandem mass spectrometry (MS/MS) combined with bioinformatics tools have enabled fast and systematic protein identification based on peptide-to-spectrum matches. However, it remains challenging to obtain accurate identification of endogenous peptides, such as neuropeptides, peptide hormones, peptide pheromones, venom ...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Hayakawa E,Menschaert G,De Bock PJ,Luyten W,Gevaert K,Baggerman G,Schoofs L

    更新日期:2013-12-06 00:00:00

  • Elucidating Escherichia coli Proteoform Families Using Intact-Mass Proteomics and a Global PTM Discovery Database.

    abstract::A proteoform family is a group of related molecular forms of a protein (proteoforms) derived from the same gene. We have previously described a strategy to identify proteoforms and elucidate proteoform families in complex mixtures of intact proteins. The strategy is based upon measurements of two properties for each p...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Dai Y,Shortreed MR,Scalf M,Frey BL,Cesnik AJ,Solntsev S,Schaffer LV,Smith LM

    更新日期:2017-11-03 00:00:00

  • Coping with anoxia: a comprehensive proteomic and transcriptomic survey of denitrification.

    abstract::Ralstonia eutropha H16 is a denitrifying microorganism able to use nitrate and nitrite as terminal electron acceptors under oxygen deprivation. To identify proteins showing an altered expression pattern in response to oxygen supply, R. eutropha cells grown aerobically and anaerobically were compared in a comprehensive...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Kohlmann Y,Pohlmann A,Schwartz E,Zühlke D,Otto A,Albrecht D,Grimmler C,Ehrenreich A,Voigt B,Becher D,Hecker M,Friedrich B,Cramm R

    更新日期:2014-10-03 00:00:00

  • Protein profiling of human pancreatic islets by two-dimensional gel electrophoresis and mass spectrometry.

    abstract::Completion of the human genome sequence has provided scientists with powerful resources with which to explore the molecular events associated with disease states such as diabetes. Understanding the relative levels of expression of gene products, especially of proteins, and their post-translational modifications will b...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Ahmed M,Forsberg J,Bergsten P

    更新日期:2005-05-01 00:00:00

  • Response to the Comments on "Determining Allele-Specific Protein Expression (ASPE) Using a Novel Quantitative Concatamer Proteomics Method".

    abstract::Russell and colleagues deserve credit for being the first to use a QconCAT standard to simultaneously quantify both the wild-type and mutant peptides of a protein (i.e., CYP2B6) ( J. Proteome Res. 2013, 12 (12), 5934-5942. DOI: 10.1021/pr400279u). However, the rationale of their study was entirely different from ours ...

    journal_title:Journal of proteome research

    pub_type: 评论,杂志文章


    authors: Shi J,Wang X,Zhu H,Jiang H,Wang D,Nesvizhskii A,Zhu HJ

    更新日期:2019-03-01 00:00:00

  • Evaluating the conformation and binding interface of cap-binding proteins and complexes via ultraviolet photodissociation mass spectrometry.

    abstract::We report the structural analysis of cap-binding proteins using a chemical probe/ultraviolet photodissociation (UVPD) mass spectrometry strategy for evaluating solvent accessibility of proteins. Our methodology utilized a chromogenic probe (NN) to probe the exposed amine residues of wheat eukaryotic translation initia...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: O'Brien JP,Mayberry LK,Murphy PA,Browning KS,Brodbelt JS

    更新日期:2013-12-06 00:00:00

  • Proteomic and Metabolomic Analyses of Leaf from Clematis terniflora DC. Exposed to High-Level Ultraviolet-B Irradiation with Dark Treatment.

    abstract::Clematis terniflora DC. has potential pharmaceutical value; on the contrary, high-level UV-B irradiation with dark treatment led to the accumulation of secondary metabolites. Metabolomic and proteomic analyses of leaf of C. terniflora were performed to investigate the systematic response mechanisms to high-level UV-B ...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Yang B,Wang X,Gao C,Chen M,Guan Q,Tian J,Komatsu S

    更新日期:2016-08-05 00:00:00

  • A difference gel electrophoresis study on thylakoids isolated from poplar leaves reveals a negative impact of ozone exposure on membrane proteins.

    abstract::Populus tremula L. x P. alba L. (Populus x canescens (Aiton) Smith), clone INRA 717-1-B4, saplings were subjected to 120 ppb ozone exposure for 28 days. Chloroplasts were isolated, and the membrane proteins, solubilized using the detergent 1,2-diheptanoyl-sn-glycero-3-phosphocholine (DHPC), were analyzed in a differen...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Bohler S,Sergeant K,Hoffmann L,Dizengremel P,Hausman JF,Renaut J,Jolivet Y

    更新日期:2011-07-01 00:00:00

  • hEIDI: An Intuitive Application Tool To Organize and Treat Large-Scale Proteomics Data.

    abstract::Advances in high-throughput proteomics have led to a rapid increase in the number, size, and complexity of the associated data sets. Managing and extracting reliable information from such large series of data sets require the use of dedicated software organized in a consistent pipeline to reduce, validate, exploit, an...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Hesse AM,Dupierris V,Adam C,Court M,Barthe D,Emadali A,Masselon C,Ferro M,Bruley C

    更新日期:2016-10-07 00:00:00