A sulfated, phosphorylated 7 kDa secreted peptide characterized by direct analysis of cell culture media.

abstract：:The increasing occurrence of multidrug resistant tuberculosis exerts a major burden on treatment of this infectious disease. Thioridazine, previously used as a neuroleptic, is active against extensively drug resistant tuberculosis when added to other second- and third-line antibiotics. By quantitatively studying the p...

journal_title：Journal of proteome research

authors： de Keijzer J,Mulder A,de Haas PE,de Ru AH,Heerkens EM,Amaral L,van Soolingen D,van Veelen PA

更新日期：2016-06-03 00:00:00

abstract：:"Dry eye" is a multifactorial inflammatory disease affecting the ocular surface. Tear hyperosmolarity in dry eye contributes to inflammation and cell damage. Recent research efforts on dry eye have been directed toward biomarker discovery for diagnosis, response to treatment, and disease mechanisms. This study employe...

journal_title：Journal of proteome research

authors： Chen L,Li J,Guo T,Ghosh S,Koh SK,Tian D,Zhang L,Jia D,Beuerman RW,Aebersold R,Chan EC,Zhou L

更新日期：2015-09-04 00:00:00

abstract：:Advances in proteomics are continually driven by the introduction of new mass spectrometric instrumentation with improved performances. The recently introduced quadrupole Orbitrap (Q Exactive) tandem mass spectrometer allows fast acquisition of high-resolution higher-energy collisional dissociation (HCD) tandem mass s...

journal_title：Journal of proteome research

authors： Kelstrup CD,Young C,Lavallee R,Nielsen ML,Olsen JV

更新日期：2012-06-01 00:00:00

abstract：:The study of protein phosphorylation has grown exponentially in recent years, as it became evident that important cellular functions are regulated by phosphorylation and dephosphorylation of proteins on serine, threonine and tyrosine residues. The use of immobilized metal affinity chromatography (IMAC) to enrich phosp...

journal_title：Journal of proteome research

authors： Aprilita NH,Huck CW,Bakry R,Feuerstein I,Stecher G,Morandell S,Huang HL,Stasyk T,Huber LA,Bonn GK

更新日期：2005-11-01 00:00:00

abstract：:Metabolically engineered Escherichia coli has previously been used to degrade cis-1,2-dichloroethylene (cis-DCE). The strains express the six genes of an evolved toluene ortho-monooxygenase from Burkholderia cepacia G4 (TOM-Green, which formed a reactive epoxide) with either (1) gamma-glutamylcysteine synthetase (GSHI...

journal_title：Journal of proteome research

authors： Lee J,Cao L,Ow SY,Barrios-Llerena ME,Chen W,Wood TK,Wright PC

更新日期：2006-06-01 00:00:00

abstract：:Various biological activities of immunoglobulin G (IgG) including antibody-dependent cellular cytotoxicity (ADCC) are modulated by the structural features of the N-glycans in the Fc part. In this study, we describe a population of IgG1 alloantibodies which are formed during pregnancy against human platelet antigens (H...

journal_title：Journal of proteome research

authors： Wuhrer M,Porcelijn L,Kapur R,Koeleman CA,Deelder A,de Haas M,Vidarsson G

更新日期：2009-02-01 00:00:00

abstract：:Papillary thyroid carcinoma (PTC) is the most common type of thyroid cancer among women worldwide. It is confirmed mainly by fine-needle aspiration biopsy (FNAB), an invasive diagnostic method. The key proteins responsible for thyroid hormone biosynthesis are glycosylated. Hence, changes in site-specific glycosylation...

journal_title：Journal of proteome research

authors： Zhang Y,Zhao W,Zhao Y,Mao Y,Su T,Zhong Y,Wang S,Zhai R,Cheng J,Fang X,Zhu J,Yang H

更新日期：2020-07-02 00:00:00

abstract：:Plant secretory (Class III) peroxidases are redox enzymes that rely on N-glycosylation for full enzyme activity and stability. Peroxidases from palm tree leaves comprise the most stable and active plant peroxidases characterized to date. Herein, site-specific glycosylation and microheterogeneity of windmill palm tree ...

journal_title：Journal of proteome research

authors： Baker MR,Tabb DL,Ching T,Zimmerman LJ,Sakharov IY,Li QX

更新日期：2016-06-03 00:00:00

abstract：:Profiling of tissues and cell types through systematic characterization of expressed genes or proteins shows promise as a basic research tool, and has potential applications in disease diagnosis and classification. We used multidimensional protein identification protein identification technology (MudPIT) to analyze pr...

journal_title：Journal of proteome research

authors： Cagney G,Park S,Chung C,Tong B,O'Dushlaine C,Shields DC,Emili A

更新日期：2005-09-01 00:00:00

abstract：:Defined as proteins actively transported via secretion systems, secreted proteins can have radically different subcellular destinations in monoderm (Gram-positive) bacteria. From degradative enzymes in saprophytes to virulence factors in pathogens, secreted proteins are the main tools used by bacteria to interact with...

journal_title：Journal of proteome research

authors： Desvaux M,Dumas E,Chafsey I,Chambon C,Hébraud M

更新日期：2010-10-01 00:00:00

abstract：:Fulminant type 1 diabetes (FT1DM) is a relatively new clinical entity featured by acute destruction of pancreatic beta cells. Clinical consequences of FT1DM could be fatal when timely medications are not provided, suggesting the particular importance of rapid and accurate diagnosis. Here we report a serum metabonomics...

journal_title：Journal of proteome research

authors： Lu J,Zhou J,Bao Y,Chen T,Zhang Y,Zhao A,Qiu Y,Xie G,Wang C,Jia W,Jia W

更新日期：2012-09-07 00:00:00

abstract：:Protein microarrays consist of known proteins spotted onto solid substrates and are used to perform highly multivariate assessments of protein-binding interactions. Human protein arrays are routinely applied to pathogen detection, immune response biomarker profiling, and antibody specificity profiling. Here, we descri...

journal_title：Journal of proteome research

authors： Feng F,Ataca ST,Ran M,Wang Y,Breen M,Kepler TB

更新日期：2020-07-02 00:00:00

abstract：:Diabetes mellitus is a complex polygenic disease caused by gene-environment interactions with multiple complications, and metabonomic analysis is crucial for pathogenesis, early diagnosis, and timely interventions. Here, we comprehensively analyzed the dynamic metabolic changes in rat urine and plasma, which were indu...

journal_title：Journal of proteome research

authors： Xu W,Wu J,An Y,Xiao C,Hao F,Liu H,Wang Y,Tang H

更新日期：2012-06-01 00:00:00

abstract：:Post-translational nitration of proteins was analyzed by capillary reversed-phase high-performance liquid chromatography (RP-HPLC) on-line interfaced to electrospray ionization mass spectrometry (ESI--MS) or tandem mass spectrometry (ESI--MS/MS). Both methods were compared using a tryptic digest of bovine serum albumi...

journal_title：Journal of proteome research

authors： Walcher W,Franze T,Weller MG,Pöschl U,Huber CG

更新日期：2003-09-01 00:00:00

abstract：:The CB1 and CB2 cannabinoid receptors belong to the GPCR superfamily and are associated with a variety of physiological and pathophysiological processes. Both receptors, with several lead compounds at different phases of development, are potentially useful targets for drug discovery. For this reason, fully elucidating...

journal_title：Journal of proteome research

authors： Zvonok N,Yaddanapudi S,Williams J,Dai S,Dong K,Rejtar T,Karger BL,Makriyannis A

更新日期：2007-06-01 00:00:00

abstract：:Acute stress may trigger systemic biochemical and physiological changes in living organisms, leading to a rapid loss of homeostasis, which can be gradually reinstated by self-regulatory mechanisms and/or drug intervention strategy. However, such a sophisticated metabolic regulatory process has so far been poorly under...

journal_title：Journal of proteome research

authors： Wang X,Su M,Qiu Y,Ni Y,Zhao T,Zhou M,Zhao A,Yang S,Zhao L,Jia W

更新日期：2007-09-01 00:00:00

abstract：:A proper sample preparation, in particular, abundant protein removal is crucial in the characterization of low-abundance proteins including those harboring post-translational modifications. In human cerebrospinal fluid (CSF), approximately 80% of proteins originate from serum, and removal of major proteins is necessar...

journal_title：Journal of proteome research

authors： Ogata Y,Charlesworth MC,Muddiman DC

更新日期：2005-05-01 00:00:00

abstract：:Histones regulate a variety of chromatin templated events by their post-translational modifications (PTMs). Although there are extensive reports on the PTMs of canonical histones, the information on the histone variants remains very scanty. Here, we report the identification of different PTMs, such as acetylation, met...

journal_title：Journal of proteome research

authors： Pentakota SK,Sandhya S,P Sikarwar A,Chandra N,Satyanarayana Rao MR

更新日期：2014-12-05 00:00:00

abstract：:The two key steps for analyzing proteomic data generated by high-resolution MS are database searching and postprocessing. While the two steps are interrelated, studies on their combinatory effects and the optimization of these procedures have not been adequately conducted. Here, we investigated the performance of thre...

journal_title：Journal of proteome research

authors： Tu C,Sheng Q,Li J,Ma D,Shen X,Wang X,Shyr Y,Yi Z,Qu J

更新日期：2015-11-06 00:00:00

abstract：:Vascular leakage is a hallmark of severe dengue infection. Although extensive studies have been conducted during the past several decades, the molecular mechanisms underlying vascular leakage in dengue shock syndrome (DSS) remain unclear. We thus performed a proteomics study to characterize responses in human endothel...

journal_title：Journal of proteome research

authors： Kanlaya R,Pattanakitsakul SN,Sinchaikul S,Chen ST,Thongboonkerd V

更新日期：2009-05-01 00:00:00

abstract：:Porphyromonas gingivalis is a keystone periodontal pathogen that has been associated with autoimmune disorders. The cell surface proteases Lys-gingipain (Kgp) and Arg-gingipains (RgpA and RgpB) are major virulence factors, and their proteolytic activity is enhanced by small peptides such as glycylglycine (GlyGly). The...

journal_title：Journal of proteome research

authors： Zhang L,Veith PD,Huq NL,Chen YY,Seers CA,Cross KJ,Gorasia DG,Reynolds EC

更新日期：2018-08-03 00:00:00

abstract：:Butyrate, a fermentation product of the large bowel microflora, is potentially protective against the development of colorectal cancer. In vitro, butyrate has been shown to induce apoptosis and inhibit proliferation in numerous cancer cell lines, including colorectal cancer. Although these tumor suppressing properties...

journal_title：Journal of proteome research

authors： Fung KY,Lewanowitsch T,Henderson ST,Priebe I,Hoffmann P,McColl SR,Lockett T,Head R,Cosgrove LJ

更新日期：2009-03-01 00:00:00

abstract：:Nicotinamide adenine dinucleotide (NAD) is a coenzyme found in all living cells and mediates multiple cellular signaling pathways. In the present study, a 35% decrease of cellular NAD level is achieved by stable expression of the N-terminal truncated CD38, a NAD hydrolase. CD38-expressing (CD38(+)) cells have the lowe...

journal_title：Journal of proteome research

authors： Hu Y,Wang H,Wang Q,Deng H

更新日期：2014-02-07 00:00:00

abstract：:Coronary artery disease is one of the most frequent causes of morbidity and mortality worldwide. It is even more prevalent in patients with type 2 diabetes mellitus who suffer from obesity and increased accumulation of epicardial fat with a possible contributing role in the development of coronary artery disease. We p...

journal_title：Journal of proteome research

authors： Tomášová P,Čermáková M,Pelantová H,Vecka M,Kratochvílová H,Lipš M,Lindner J,Ivák P,Netuka I,Šedivá B,Haluzík M,Kuzma M

更新日期：2020-10-02 00:00:00

abstract：:Protein phosphorylation is a major mechanism of signal transduction in bacteria. Here, we analyzed the proteome and phosphoproteome of a wild-type strain of the food-borne pathogen Listeria monocytogenes that was grown in either chemically defined medium or rich medium containing glucose. We then compared these result...

journal_title：Journal of proteome research

authors： Misra SK,Moussan Désirée Aké F,Wu Z,Milohanic E,Cao TN,Cossart P,Deutscher J,Monnet V,Archambaud C,Henry C

更新日期：2014-12-05 00:00:00

abstract：:The endothelium plays a critical role in orchestrating the inflammatory response seen during sepsis. Many of the inflammatory effects of Gram-negative sepsis are elicited by lipopolysaccharide (LPS), a glycolipid component of bacterial cell walls. Lipid-rich microdomains have been shown to concentrate components of th...

journal_title：Journal of proteome research

authors： Karsan A,Blonder J,Law J,Yaquian E,Lucas DA,Conrads TP,Veenstra T

更新日期：2005-03-01 00:00:00

abstract：:An individually darkened leaf model was used to study protein changes in the Arabidopsis mutant stay-green1 (sgr1) to partially mimic the process of leaf covering senescence that occurs naturally in the shaded rosettes of Arabidopsis plants. Utilizing this controlled and predictable induced senescence model has allowe...

journal_title：Journal of proteome research

authors： Grassl J,Pružinská A,Hörtensteiner S,Taylor NL,Millar AH

更新日期：2012-11-02 00:00:00

abstract：:In an attempt to identify prostate cancer biomarkers with greater diagnostic and prognostic capabilities, we have developed an integrative proteomic discovery workflow focused on N-linked glycoproteins that refines the target selection process. In this work, hydrazide-based chemistry was used to identify N-linked glyc...

journal_title：Journal of proteome research

authors： Whitmore TE,Peterson A,Holzman T,Eastham A,Amon L,McIntosh M,Ozinsky A,Nelson PS,Martin DB

更新日期：2012-05-04 00:00:00

abstract：:Streptococcus agalactiae causes severe systemic infections in human and fish. In the present study, we established a pathogen-plasma interaction model by which we explored how S. agalactiae evaded serum-mediated killing. We found that S. agalactiae grew faster in the presence of yellow grouper plasma than in the absen...

journal_title：Journal of proteome research

authors： Wang Z,Li MY,Peng B,Cheng ZX,Li H,Peng XX

更新日期：2016-07-01 00:00:00

abstract：:Pancreas ductal adenocarcinoma (PDAC) remains a deadly malignancy with poor early diagnostic and no effective therapy. Although several proteomic studies have performed comparative analysis between normal and malignant tissues, there is a lack of clear characterization of proteins that could be of clinical value. Syst...

journal_title：Journal of proteome research

authors： Turtoi A,Musmeci D,Wang Y,Dumont B,Somja J,Bevilacqua G,De Pauw E,Delvenne P,Castronovo V

更新日期：2011-09-02 00:00:00