Abstract：
:In type-1 diabetes mellitus (T1DM) with diabetic nephropathy (DN), accumulation of abnormal proteins in the kidney and other tissues may derive from constitutive alterations of intracellular protein recognition, assembly, and turnover. We characterized the proteins involved in these functions in cultured skin fibroblasts from long-term T1DM patients with [DN+] or without [DN-] nephropathy but similar metabolic control, and from matched healthy subjects. 2-D gel electrophoresis and MS-MALDI analysis were employed. The [DN+] T1DM patients, compared with the two other groups, exhibited increased abundance of a high-molecular weight isoform of protein disulphide-isomerase A3 and a decrease of two low-molecular weight isoforms. They also had increased levels of heat shock protein (HSP) 60 kDa isoform #A4, of HSP71 kDa isoform #A30, and of HSP27 kDa isoform #6, whereas the HSP27 kDa isoforms #A90 and #A71 were decreased. Cathepsin beta-2 (#40), the cation-independent mannose 6-phosphate receptor binding protein 1 (CIMPR) (#A27), and annexin 2 (#A9) were also decreased in the [DN+] T1DM patients, whereas the RNA-binding protein regulatory subunity (#38) and the translationally-controlled tumor protein (TCTP) (#A45) were increased. These changes of chaperone-like proteins in fibroblasts may highlight those of the kidney and be patho-physiologically related to the development of nephropathy in T1DM.
journal_name
J Proteome Resjournal_title
Journal of proteome researchauthors
Tessari P,Puricelli L,Iori E,Arrigoni G,Vedovato M,James P,Coracina A,Millioni Rdoi
10.1021/pr060443nsubject
Has Abstractpub_date
2007-03-01 00:00:00pages
976-86issue
3eissn
1535-3893issn
1535-3907journal_volume
6pub_type
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更新日期：2014-05-02 00:00:00
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更新日期：2012-12-07 00:00:00
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journal_title：Journal of proteome research
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doi：10.1021/pr7006285
更新日期：2008-07-01 00:00:00
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pub_type： 杂志文章,随机对照试验
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更新日期：2017-06-02 00:00:00
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更新日期：2011-01-07 00:00:00
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journal_title：Journal of proteome research
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doi：10.1021/pr401108d
更新日期：2014-03-07 00:00:00
abstract：:Endometrial cancer (EC) is the most frequent gynecological cancer. Tumor dissemination affecting ∼20% of EC patients is characterized at the primary carcinoma by epithelial-to-mesenchymal transition (EMT) associated with myometrial infiltration. At distant sites, the interaction of circulating tumor cells (CTCs) with ...
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