Abstract:
:Profiling of tissues and cell types through systematic characterization of expressed genes or proteins shows promise as a basic research tool, and has potential applications in disease diagnosis and classification. We used multidimensional protein identification protein identification technology (MudPIT) to analyze proteomes for enriched nuclear extracts of eight human tissues: brain, heart, liver, lung, muscle, pancreas, spleen, and testis. We show that the method is approximately 80% reproducible. We address issues of relative abundance, tissue-specificity, and selectivity, and the significance of proteins whose expression does not correlate with that of the corresponding mRNA. Surprisingly, most proteins are detected in a single tissue. These proteins tend to fulfill specialist (and potentially tissue-specific) functions compared to proteins expressed in two or more tissues.
journal_name
J Proteome Resjournal_title
Journal of proteome researchauthors
Cagney G,Park S,Chung C,Tong B,O'Dushlaine C,Shields DC,Emili Adoi
10.1021/pr0500354keywords:
subject
Has Abstractpub_date
2005-09-01 00:00:00pages
1757-67issue
5eissn
1535-3893issn
1535-3907journal_volume
4pub_type
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