Abstract：
:Hundreds of ribosomally synthesized cyclopeptides have been isolated from all domains of life, the vast majority having been reported in the last 15 years. Studies of cyclic peptides have highlighted their exceptional potential both as stable drug scaffolds and as biomedicines in their own right. Despite this, computational techniques for cyclopeptide identification are still in their infancy, with many such peptides remaining uncharacterized. Tandem mass spectrometry has occupied a niche role in cyclopeptide identification, taking over from traditional techniques such as nuclear magnetic resonance spectroscopy (NMR). MS/MS studies require only picogram quantities of peptide (compared to milligrams for NMR studies) and are applicable to complex samples, abolishing the requirement for time-consuming chromatographic purification. While database search tools such as Sequest and Mascot have become standard tools for the MS/MS identification of linear peptides, they are not applicable to cyclopeptides, due to the parent mass shift resulting from cyclization and different fragmentation patterns of cyclic peptides. In this paper, we describe the development of a novel database search methodology to aid in the identification of cyclopeptides by mass spectrometry and evaluate its utility in identifying two peptide rings from Helianthus annuus, a bacterial cannibalism factor from Bacillus subtilis, and a θ-defensin from Rhesus macaque.
journal_name
J Proteome Resjournal_title
Journal of proteome researchauthors
Mohimani H,Liu WT,Mylne JS,Poth AG,Colgrave ML,Tran D,Selsted ME,Dorrestein PC,Pevzner PAdoi
10.1021/pr200323asubject
Has Abstractpub_date
2011-10-07 00:00:00pages
4505-12issue
10eissn
1535-3893issn
1535-3907journal_volume
10pub_type
杂志文章abstract：:Mammary tumors are a major health threat to women and female dogs. In both, metastasis of the primary tumor to distant organs is the most common cause of tumor-related death. Nevertheless, the molecular mechanisms of tumor metastasis are far from being understood, and it is still unknown why some human and canine carc...
journal_title：Journal of proteome research
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journal_title：Journal of proteome research
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journal_title：Journal of proteome research
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journal_title：Journal of proteome research
pub_type： 杂志文章
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更新日期：2007-06-01 00:00:00
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journal_title：Journal of proteome research
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更新日期：2013-03-01 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr5003142
更新日期：2014-06-06 00:00:00
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journal_title：Journal of proteome research
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更新日期：2018-01-05 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
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更新日期：2009-02-01 00:00:00
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journal_title：Journal of proteome research
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doi：10.1021/pr060504h
更新日期：2007-03-01 00:00:00
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journal_title：Journal of proteome research
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更新日期：2017-01-06 00:00:00
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更新日期：2014-11-07 00:00:00
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journal_title：Journal of proteome research
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journal_title：Journal of proteome research
pub_type： 杂志文章
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更新日期：2016-06-03 00:00:00
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journal_title：Journal of proteome research
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更新日期：2020-04-03 00:00:00
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journal_title：Journal of proteome research
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更新日期：2011-02-04 00:00:00
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更新日期：2014-03-07 00:00:00
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