Abstract：
:Dipetalogaster maxima is a blood-sucking Hemiptera that inhabits sylvatic areas in Mexico. It usually takes its blood meal from lizards, but following human population growth, it invaded suburban areas, feeding also on humans and domestic animals. Hematophagous insect salivary glands produce potent pharmacologic compounds that counteract host hemostasis, including anticlotting, antiplatelet, and vasodilatory molecules. To obtain further insight into the salivary biochemical and pharmacologic complexity of this insect, a cDNA library from its salivary glands was randomly sequenced. Salivary proteins were also submitted to one- and two-dimensional gel electrophoresis (1DE and 2DE) followed by mass spectrometry analysis. We present the analysis of a set of 2728 cDNA sequences, 1375 of which coded for proteins of a putative secretory nature. The saliva 2DE proteome displayed approximately 150 spots. The mass spectrometry analysis revealed mainly lipocalins, pallidipins, antigen 5-like proteins, and apyrases. The redundancy of sequence identification of saliva-secreted proteins suggests that proteins are present in multiple isoforms or derive from gene duplications.
journal_name
J Proteome Resjournal_title
Journal of proteome researchauthors
Assumpção TC,Charneau S,Santiago PB,Francischetti IM,Meng Z,Araújo CN,Pham VM,Queiroz RM,de Castro CN,Ricart CA,Santana JM,Ribeiro JMdoi
10.1021/pr100866hsubject
Has Abstractpub_date
2011-02-04 00:00:00pages
669-79issue
2eissn
1535-3893issn
1535-3907journal_volume
10pub_type
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