In-depth proteomic and glycomic analysis of the adult-stage Cooperia oncophora excretome/secretome.

Abstract:

:Cooperia oncophora is one of the most common intestinal parasitic nematodes in cattle worldwide. To date, C. oncophora infections are treated using broad-spectrum anthelmintics. However, during the past decade, reports of anthelmintic resistance in this parasite species have emerged worldwide, necessitating new avenues for its control, possibly through vaccination. In this frame, we analyzed the adult-stage C. oncophora excretome/secretome (ES), covering both the protein and glycan components, since this fraction constitutes the primary interface between parasite and host and may hold potential vaccine candidates. Two-dimensional gel electrophoretic separation of the ES material enabled the MALDI-TOF mass spectrometry (MS)-directed identification of 12 distinct proteins, grouped in three separate molecular weight fractions: (i) a high molecular weight fraction consisting of a double-domain activation-associated secreted protein (ASP), (ii) a midmolecular weight fraction predominantly containing a single-domain ASP, a thioredoxin peroxidase and innexin, and (iii) a low molecular weight protein pool essentially holding two distinct low molecular weight antigens. Further MS-driven glycan analysis mapped a variety of N-glycans to the midmolecular weight single-domain ASP, with Man6GlcNAc2 oligomannosyl glycans as the major species. The predominance of the nonglycosylated double-domain ASP in the high-molecular weight fraction renders it ideal for advancement toward vaccine trials and development.

journal_name

J Proteome Res

authors

Borloo J,De Graef J,Peelaers I,Nguyen DL,Mitreva M,Devreese B,Hokke CH,Vercruysse J,Claerebout E,Geldhof P

doi

10.1021/pr400114y

subject

Has Abstract

pub_date

2013-09-06 00:00:00

pages

3900-11

issue

9

eissn

1535-3893

issn

1535-3907

journal_volume

12

pub_type

杂志文章
  • The Muscle Metabolome Differs between Healthy and Frail Older Adults.

    abstract::Populations around the world are aging rapidly. Age-related loss of physiological functions negatively affects quality of life. A major contributor to the frailty syndrome of aging is loss of skeletal muscle. In this study we assessed the skeletal muscle biopsy metabolome of healthy young, healthy older and frail olde...

    journal_title:Journal of proteome research

    pub_type: 杂志文章

    doi:10.1021/acs.jproteome.5b00840

    authors: Fazelzadeh P,Hangelbroek RW,Tieland M,de Groot LC,Verdijk LB,van Loon LJ,Smilde AK,Alves RD,Vervoort J,Müller M,van Duynhoven JP,Boekschoten MV

    更新日期:2016-02-05 00:00:00

  • Identification of autocrine growth factors secreted by CHO cells for applications in single-cell cloning media.

    abstract::Chinese hamster ovary (CHO) cell lines are widely used for the expression of therapeutic recombinant proteins, including monoclonal antibodies and other biologics. For manufacturing, cells derived from a single-cell clone are typically used to ensure product consistency. Presently, fetal bovine serum (FBS) is commonly...

    journal_title:Journal of proteome research

    pub_type: 杂志文章

    doi:10.1021/pr400352n

    authors: Lim UM,Yap MG,Lim YP,Goh LT,Ng SK

    更新日期:2013-07-05 00:00:00

  • Liquid- and gas-phase nitration of bovine serum albumin studied by LC-MS and LC-MS/MS using monolithic columns.

    abstract::Post-translational nitration of proteins was analyzed by capillary reversed-phase high-performance liquid chromatography (RP-HPLC) on-line interfaced to electrospray ionization mass spectrometry (ESI--MS) or tandem mass spectrometry (ESI--MS/MS). Both methods were compared using a tryptic digest of bovine serum albumi...

    journal_title:Journal of proteome research

    pub_type: 杂志文章

    doi:10.1021/pr034034s

    authors: Walcher W,Franze T,Weller MG,Pöschl U,Huber CG

    更新日期:2003-09-01 00:00:00

  • Circulating Lamin B1 (LMNB1) biomarker detects early stages of liver cancer in patients.

    abstract::Hepatocellular carcinoma (HCC) is a major liver malignancy possessing a high mortality rate and is particularly prevalent in China and Asia. While surgery is the most effective treatment for liver tumor, about 80% of HCC patients are inoperable at presentation and die early due to late diagnosis. For early cancer dete...

    journal_title:Journal of proteome research

    pub_type: 杂志文章

    doi:10.1021/pr9002118

    authors: Sun S,Xu MZ,Poon RT,Day PJ,Luk JM

    更新日期:2010-01-01 00:00:00

  • Identification of Missing Proteins in Human Olfactory Epithelial Tissue by Liquid Chromatography-Tandem Mass Spectrometry.

    abstract::We performed proteomic analyses of human olfactory epithelial tissue to identify missing proteins using liquid chromatography-tandem mass spectrometry. Using a next-generation proteomic pipeline with a < 1.0% false discovery rate at the peptide and protein levels, we identified 3731 proteins, among which five were mis...

    journal_title:Journal of proteome research

    pub_type: 杂志文章

    doi:10.1021/acs.jproteome.8b00408

    authors: Hwang H,Jeong JE,Lee HK,Yun KN,An HJ,Lee B,Paik YK,Jeong TS,Yee GT,Kim JY,Yoo JS

    更新日期:2018-12-07 00:00:00

  • Retinal Mueller glial cells trigger the hallmark inflammatory process in autoimmune uveitis.

    abstract::Spontaneous equine recurrent uveitis (ERU) is an incurable autoimmune disease affecting the eye. Although retinal-autoantigen specific T-helper 1 cells have been demonstrated to trigger disease progression and relapses, the molecular processes leading to retinal degeneration and consequent blindness remain unknown. To...

    journal_title:Journal of proteome research

    pub_type: 杂志文章

    doi:10.1021/pr060668y

    authors: Hauck SM,Schoeffmann S,Amann B,Stangassinger M,Gerhards H,Ueffing M,Deeg CA

    更新日期:2007-06-01 00:00:00

  • Introduction of the disulfide proteome: application of a technique for the analysis of plant storage proteins as well as allergens.

    abstract::Redox regulation plays an important role across a broad spectrum of biology. Accumulating evidence suggests that thioredoxin, a widely distributed redox enzyme, participates in the redox control of numerous target proteins, thus, playing a key role as a signaling intermediate that senses the metabolic state or environ...

    journal_title:Journal of proteome research

    pub_type: 杂志文章,评审

    doi:10.1021/pr8003453

    authors: Yano H,Kuroda S

    更新日期:2008-08-01 00:00:00

  • Highly altered protein expression profile in the adriamycin resistant MCF-7 cell line.

    abstract::The protein expression pattern in the cytosol fraction of the adriamycin resistant MCF-7 cell line (MCF-7/ADR) was compared to that of the parental MCF-7 cell line using two-dimensional gel electrophoresis and mass spectrometry. Twenty proteins with altered abundances were identified and studied in MCF-7/ADR. Both up ...

    journal_title:Journal of proteome research

    pub_type: 杂志文章

    doi:10.1021/pr0340577

    authors: Gehrmann ML,Fenselau C,Hathout Y

    更新日期:2004-05-01 00:00:00

  • Identifying proteins in zebrafish embryos using spectral libraries generated from dissected adult organs and tissues.

    abstract::Spectral libraries provide a sensitive and accurate method for identifying peptides from tandem mass spectra, complementary to searching genome-derived databases or sequencing de novo. Their application requires comprehensive libraries including peptides from low-abundant proteins. Here we describe a method for constr...

    journal_title:Journal of proteome research

    pub_type: 杂志文章

    doi:10.1021/pr4010585

    authors: van der Plas-Duivesteijn SJ,Mohammed Y,Dalebout H,Meijer A,Botermans A,Hoogendijk JL,Henneman AA,Deelder AM,Spaink HP,Palmblad M

    更新日期:2014-03-07 00:00:00

  • Single-step procedure for the isolation of proteins at near-native conditions from mammalian tissue for proteomic analysis on antibody microarrays.

    abstract::The process of extracting comprehensive proteome representations is a crucial step for many proteomic studies. While antibody microarrays are an evolving and promising methodology in proteomics, the issue of protein extraction from tissues for this kind of analysis has never been addressed. Here, we describe a single-...

    journal_title:Journal of proteome research

    pub_type: 杂志文章

    doi:10.1021/pr900844q

    authors: Alhamdani MS,Schröder C,Werner J,Giese N,Bauer A,Hoheisel JD

    更新日期:2010-02-05 00:00:00

  • Relative and Quantitative Phosphoproteome Analysis of Macrophages in Response to Infection by Virulent and Avirulent Mycobacteria Reveals a Distinct Role of the Cytosolic RNA Sensor RIG-I in Mycobacterium tuberculosis Pathogenesis.

    abstract::Comparative phosphoproteomics of Mycobacterium tuberculosis (Mtb)- and Mycobacterium bovis BCG (BCG)-infected macrophages could be instrumental in understanding the characteristic post-translational modifications of host proteins and their subsequent involvement in determining Mtb pathogenesis. To identify proteins ac...

    journal_title:Journal of proteome research

    pub_type: 杂志文章

    doi:10.1021/acs.jproteome.9b00895

    authors: Choudhary E,Bullen CK,Goel R,Singh AK,Praharaj M,Thakur P,Dhiman R,Bishai WR,Agarwal N

    更新日期:2020-06-05 00:00:00

  • Shotgun protein profile of human adipose tissue and its changes in relation to systemic amyloidoses.

    abstract::In systemic amyloidosis, accumulation of misfolded proteins as extracellular amyloid fibrils in tissues causes severe organ dysfunction, but the molecular events of tissue damage related to amyloid deposition are still largely unknown. Through the use of the MudPIT proteomic approach, comprehensive protein profiles of...

    journal_title:Journal of proteome research

    pub_type: 杂志文章

    doi:10.1021/pr400583h

    authors: Brambilla F,Lavatelli F,Di Silvestre D,Valentini V,Palladini G,Merlini G,Mauri P

    更新日期:2013-12-06 00:00:00

  • Investigating the dynamic nature of the interactions between nuclear proteins and histones upon DNA damage using an immobilized peptide chemical proteomics approach.

    abstract::As a result of the complexity and dynamic range of the cellular proteome, including mutual interactions and interactions with other molecules, focused proteomic approaches are important to study subsets of physiologically important proteins. In one such approach, a small molecule or part of a protein is immobilized on...

    journal_title:Journal of proteome research

    pub_type: 杂志文章

    doi:10.1021/pr060278b

    authors: Dirksen EH,Pinkse MW,Rijkers DT,Cloos J,Liskamp RM,Slijper M,Heck AJ

    更新日期:2006-09-01 00:00:00

  • Optimizing performance of glycopeptide capture for plasma proteomics.

    abstract::Selective capture of glycopolypeptides followed by release and analysis of the former glycosylation-site peptides has been shown to have promise for reducing the complexity of body fluids such as blood for biomarker discovery. In this work, a protocol based on capture of polypeptides containing a N-linked carbohydrate...

    journal_title:Journal of proteome research

    pub_type: 杂志文章

    doi:10.1021/pr900845m

    authors: Berven FS,Ahmad R,Clauser KR,Carr SA

    更新日期:2010-04-05 00:00:00

  • Suppression of peptide sample losses in autosampler vials.

    abstract::Protein or peptide sample losses could accompany all steps of the proteomic analysis workflow. We focused on suppression of sample adsorptive losses during sample storage in autosampler vials. We examined suppression capabilities of six different sample injection solutions and seven types of autosampler vial surfaces ...

    journal_title:Journal of proteome research

    pub_type: 杂志文章

    doi:10.1021/pr400183v

    authors: Stejskal K,Potěšil D,Zdráhal Z

    更新日期:2013-06-07 00:00:00

  • Alterations of Brain Quantitative Proteomics Profiling Revealed the Molecular Mechanisms of Diosgenin against Cerebral Ischemia Reperfusion Effects.

    abstract::Diosgenin (DIO), the starting material for the synthesis of steroidal anti-inflammatory drugs in the pharmaceutical industry, has been previously demonstrated to display pharmaceutical effects against cerebral ischemic reperfusion (I/R). However, the alterations of brain proteome profiles underlying this treatment rem...

    journal_title:Journal of proteome research

    pub_type: 杂志文章

    doi:10.1021/acs.jproteome.9b00667

    authors: Zhang X,Wang X,Khurm M,Zhan G,Zhang H,Ito Y,Guo Z

    更新日期:2020-03-06 00:00:00

  • Site-Specific N-Glycosylation Characterization of Windmill Palm Tree Peroxidase Using Novel Tools for Analysis of Plant Glycopeptide Mass Spectrometry Data.

    abstract::Plant secretory (Class III) peroxidases are redox enzymes that rely on N-glycosylation for full enzyme activity and stability. Peroxidases from palm tree leaves comprise the most stable and active plant peroxidases characterized to date. Herein, site-specific glycosylation and microheterogeneity of windmill palm tree ...

    journal_title:Journal of proteome research

    pub_type: 杂志文章

    doi:10.1021/acs.jproteome.6b00205

    authors: Baker MR,Tabb DL,Ching T,Zimmerman LJ,Sakharov IY,Li QX

    更新日期:2016-06-03 00:00:00

  • Integrative proteome and transcriptome analysis of extramedullary erythropoiesis and its reversal by transferrin treatment in a mouse model of beta-thalassemia.

    abstract::Beta-thalassemia results from mutations of the β-hemoglobin (Hbb) gene and reduced functional Hbb synthesis. Excess α-Hb causes globin chain aggregation, oxidation, cytoskeletal damage, and increased red blood cell clearance. These events result in anemia, altered iron homeostasis, and expansion of extramedullary eryt...

    journal_title:Journal of proteome research

    pub_type: 杂志文章

    doi:10.1021/pr5010778

    authors: Vallelian F,Gelderman-Fuhrmann MP,Schaer CA,Puglia M,Opitz L,Baek JH,Vostal J,Buehler PW,Schaer DJ

    更新日期:2015-02-06 00:00:00

  • Challenges in Peptide-Spectrum Matching: A Robust and Reproducible Statistical Framework for Removing Low-Accuracy, High-Scoring Hits.

    abstract::Workflows for large-scale (MS)-based shotgun proteomics can potentially lead to costly errors in the form of incorrect peptide-spectrum matches (PSMs). To improve the robustness of these workflows, we have investigated the use of the precursor mass discrepancy (PMD) to detect and filter potentially false PSMs that hav...

    journal_title:Journal of proteome research

    pub_type: 杂志文章

    doi:10.1021/acs.jproteome.9b00478

    authors: Hubler SL,Kumar P,Mehta S,Easterly C,Johnson JE,Jagtap PD,Griffin TJ

    更新日期:2020-01-03 00:00:00

  • Predicting eukaryotic protein subcellular location by fusing optimized evidence-theoretic K-Nearest Neighbor classifiers.

    abstract::Facing the explosion of newly generated protein sequences in the post genomic era, we are challenged to develop an automated method for fast and reliably annotating their subcellular locations. Knowledge of subcellular locations of proteins can provide useful hints for revealing their functions and understanding how t...

    journal_title:Journal of proteome research

    pub_type: 杂志文章

    doi:10.1021/pr060167c

    authors: Chou KC,Shen HB

    更新日期:2006-08-01 00:00:00

  • Proteomics of Huntington's disease-affected human embryonic stem cells reveals an evolving pathology involving mitochondrial dysfunction and metabolic disturbances.

    abstract::Huntington's disease (HD) is an autosomal dominant neurodegenerative disorder caused by a mutation in the Huntingtin gene, where excessive (≥ 36) CAG repeats encode for glutamine expansion in the huntingtin protein. Research using mouse models and human pathological material has indicated dysfunctions in a myriad of s...

    journal_title:Journal of proteome research

    pub_type: 杂志文章

    doi:10.1021/pr500649m

    authors: McQuade LR,Balachandran A,Scott HA,Khaira S,Baker MS,Schmidt U

    更新日期:2014-12-05 00:00:00

  • Proteomics in Influenza Research: The Emerging Role of Posttranslational Modifications.

    abstract::Influenza viruses continue evolving and have the ability to cause a global pandemic, so it is very important to elucidate its pathogenesis and find new treatment methods. In recent years, proteomics has made important contributions to describing the dynamic interaction between influenza viruses and their hosts, especi...

    journal_title:Journal of proteome research

    pub_type: 杂志文章

    doi:10.1021/acs.jproteome.0c00778

    authors: Zhang J,Peng Q,Zhao W,Sun W,Yang J,Liu N

    更新日期:2021-01-01 00:00:00

  • A limited screen for protein interactions reveals new roles for protein phosphatase 1 in cell cycle control and apoptosis.

    abstract::Protein phosphatase 1 (PP1) catalytic subunits typically combine with other proteins that modulate their activity, direct them to distinct substrates, or serve as substrates for PP1. More than 50 PP1-interacting proteins (PIPs) have been identified so far. Given there are approximately 10 000 phosphoproteins in mammal...

    journal_title:Journal of proteome research

    pub_type: 杂志文章

    doi:10.1021/pr060504h

    authors: Flores-Delgado G,Liu CW,Sposto R,Berndt N

    更新日期:2007-03-01 00:00:00

  • High-throughput middle-down analysis using an orbitrap.

    abstract::This report demonstrates the application of a capillary LC-LTQ-orbitrap system to provide automated middle-down analysis of proteolytic peptides in the mass range 3000 to 10,000 Da. The novel workflow combines an underutilized method in the orbitrap-high resolution, mass-accurate product ion measurements-with software...

    journal_title:Journal of proteome research

    pub_type: 杂志文章

    doi:10.1021/pr1000994

    authors: Cannon J,Lohnes K,Wynne C,Wang Y,Edwards N,Fenselau C

    更新日期:2010-08-06 00:00:00

  • Pleural effusion lipoproteins measured by NMR spectroscopy for diagnosis of exudative pleural effusions: a novel tool for pore-size estimation.

    abstract::High-resolution proton nuclear magnetic resonance (NMR) spectrometry of biofluids has been increasingly used in laboratory diagnosis of various diseases. In this study, we extended the use of (1)H NMR spectroscopy for laboratory diagnosis of exudative pleural effusions using pleural fluids. We compared this new NMR-ba...

    journal_title:Journal of proteome research

    pub_type: 杂志文章

    doi:10.1021/pr5004856

    authors: Lam CW,Law CY

    更新日期:2014-09-05 00:00:00

  • Identification of Yersinia pestis and Escherichia coli strains by whole cell and outer membrane protein extracts with mass spectrometry-based proteomics.

    abstract::Whole cell protein and outer membrane protein (OMP) extracts were compared for their ability to differentiate and delineate the correct database organism to an experimental sample and for the degree of dissimilarity to the nearest neighbor database organism strains. These extracts were isolated from pathogenic and non...

    journal_title:Journal of proteome research

    pub_type: 杂志文章

    doi:10.1021/pr100402y

    authors: Jabbour RE,Wade MM,Deshpande SV,Stanford MF,Wick CH,Zulich AW,Snyder AP

    更新日期:2010-07-02 00:00:00

  • Dual-source mass spectrometer with MALDI-LIT-ESI configuration.

    abstract::A novel linear ion trap (LIT) mass spectrometer with dual matrix-assisted laser desorption/ionization (MALDI) and electrospray ionization (ESI) ionization sources has been built in the MALDI-LIT-ESI configuration. The design features two independent ion source/ion optical channels connected to opposite ends of a singl...

    journal_title:Journal of proteome research

    pub_type: 杂志文章

    doi:10.1021/pr060514i

    authors: Smith SA,Blake TA,Ifa DR,Cooks RG,Ouyang Z

    更新日期:2007-02-01 00:00:00

  • Impact of the core components of the phosphoenolpyruvate-carbohydrate phosphotransferase system, HPr and EI, on differential protein expression in Ralstonia eutropha H16.

    abstract::In Ralstonia eutropha H16, seven genes encoding proteins being involved in the phosphoenolpyruvate-carbohydrate phosphotransferase system (PEP-PTS) were identified. In order to provide more insights into the poly(3-hydroxybutyrate) (PHB)-leaky phenotype of the HPr/EI deletion mutants H16ΔptsH, H16ΔptsI, and H16ΔptsHI ...

    journal_title:Journal of proteome research

    pub_type: 杂志文章

    doi:10.1021/pr300042f

    authors: Kaddor C,Voigt B,Hecker M,Steinbüchel A

    更新日期:2012-07-06 00:00:00

  • Characterization of cancer associated mucin type O-glycans using the exchange sialylation properties of mammalian sialyltransferase ST3Gal-II.

    abstract::Our previous studies suggest that the α2,3sialylated T-antigen (NeuAcα2,3Galβ1,3GalNac-) and associated glycan structures are likely to be elevated during cancer. An easy and reliable strategy to label mucinous glycans that contain such carbohydrates can enable the identification of novel glycoproteins that are cancer...

    journal_title:Journal of proteome research

    pub_type: 杂志文章

    doi:10.1021/pr201108q

    authors: Chandrasekaran EV,Xue J,Xia J,Locke RD,Patil SA,Neelamegham S,Matta KL

    更新日期:2012-04-06 00:00:00

  • Global metabolite profiling of human colorectal cancer xenografts in mice using HPLC-MS/MS.

    abstract::Reversed-phase gradient LC-MS was used to perform untargeted metabonomic analysis on extracts of human colorectal cancer (CRC) cell lines (COLO 205, HT-29, HCT 116 and SW620) subcutaneously implanted into age-matched athymic nude male mice to study small molecule metabolic profiles and examine possible correlations wi...

    journal_title:Journal of proteome research

    pub_type: 杂志文章

    doi:10.1021/pr400260h

    authors: Loftus NJ,Lai L,Wilkinson RW,Odedra R,Wilson ID,Barnes AJ

    更新日期:2013-06-07 00:00:00