Introduction of the disulfide proteome: application of a technique for the analysis of plant storage proteins as well as allergens.

abstract：:Activation of AMP-activated protein kinase (AMPK) upon energy depletion stimulates energy production and limits energy utilization. Erythrocytes lacking AMPK are susceptible to suicidal cell death (eryptosis). A hallmark of eryptosis is cell membrane scrambling with phosphatidylserine exposure at the erythrocyte surfa...

journal_title：Journal of proteome research

authors： Zelenak C,Föller M,Velic A,Krug K,Qadri SM,Viollet B,Lang F,Macek B

更新日期：2011-04-01 00:00:00

abstract：:Reversible phosphorylations play a critical role in most biological pathways. Hence, in signaling studies great effort has been put into identification of a maximum number of phosphosites per experiment. Mass spectrometry (MS)-based phosphoproteomics approaches have been proven to be an ideal analytical method for map...

journal_title：Journal of proteome research

authors： Zarei M,Sprenger A,Metzger F,Gretzmeier C,Dengjel J

更新日期：2011-08-05 00:00:00

abstract：:Neutrophilic granulocytes play a major role in the initiation and resolution of the inflammatory response, and demonstrate significant transcriptional and translational activity. Although much was known about neutrophils prior to the introduction of proteomics, the use of MS-based methodologies has provided an unprece...

journal_title：Journal of proteome research

authors： Tomazella GG,daSilva I,Thomé CH,Greene LJ,Koehler CJ,Thiede B,Wiker HG,de Souza GA

更新日期：2010-04-05 00:00:00

abstract：:Candida albicans (C. albicans) is a worldwide cause of fungal infectious diseases. As a general post-translational modification (PTM), lysine acetylation of proteins play an important regulatory role in almost every cell. In our research, we used a high-resolution proteomic technique (LC-MS/MS) to present the comprehe...

journal_title：Journal of proteome research

authors： Zhou X,Qian G,Yi X,Li X,Liu W

更新日期：2016-08-05 00:00:00

abstract：:A novel metabolite identification strategy is presented for the combined NMR/MS analysis of complex metabolite mixtures. The approach first identifies metabolite candidates from 1D or 2D NMR spectra by NMR database query, which is followed by the determination of the masses (m/z) of their possible ions, adducts, fragm...

journal_title：Journal of proteome research

authors： Bingol K,Brüschweiler R

更新日期：2015-06-05 00:00:00

abstract：:As a potential tool for proteomics and protein characterization, in-gel cysteine- and arginine-specific cleavage is demonstrated by means of trypsin or endoproteinase Lys-C for six model proteins (lysozyme, alpha-lactalbumin, beta-lactoglobulin, ribonuclease A, albumin, and transferrin), ranging in size from 14 kDa to...

journal_title：Journal of proteome research

authors： Thevis M,Ogorzalek Loo RR,Loo JA

更新日期：2003-03-01 00:00:00

abstract：:Schistosomes are blood-dwelling helminth parasites that cause schistosomiasis, a debilitating disease resulting in inflammation and, in extreme cases, multiple organ damage. Major challenges to control the transmission persist, and the discovery of protective antigens remains of critical importance for vaccine develop...

journal_title：Journal of proteome research

authors： Neves LX,Wilson RA,Brownridge P,Harman VM,Holman SW,Beynon RJ,Eyers CE,DeMarco R,Castro-Borges W

更新日期：2020-01-03 00:00:00

abstract：:Populations around the world are aging rapidly. Age-related loss of physiological functions negatively affects quality of life. A major contributor to the frailty syndrome of aging is loss of skeletal muscle. In this study we assessed the skeletal muscle biopsy metabolome of healthy young, healthy older and frail olde...

journal_title：Journal of proteome research

authors： Fazelzadeh P,Hangelbroek RW,Tieland M,de Groot LC,Verdijk LB,van Loon LJ,Smilde AK,Alves RD,Vervoort J,Müller M,van Duynhoven JP,Boekschoten MV

更新日期：2016-02-05 00:00:00

abstract：:We present a novel straightforward method for enrichment of N-terminal peptides, utilizing charge-based fractional diagonal chromatography (ChaFRADIC). Our method is robust, easy to operate, fast, specific, and more sensitive than existing methods, enabling the differential quantitation of 1459 nonredundant N-terminal...

journal_title：Journal of proteome research

authors： Venne AS,Vögtle FN,Meisinger C,Sickmann A,Zahedi RP

更新日期：2013-09-06 00:00:00

abstract：:The current technique used for microbial identification in hospitals is matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). However, it suffers from important limitations, in particular, for closely related species or when the database used for the identification lacks the appr...

journal_title：Journal of proteome research

authors： Dupré M,Duchateau M,Malosse C,Borges-Lima D,Calvaresi V,Podglajen I,Clermont D,Rey M,Chamot-Rooke J

更新日期：2021-01-01 00:00:00

abstract：:The identification of commercial shrimp species is a relevant issue to ensure correct labeling, maintain consumer confidence and enhance the knowledge of the captured species, benefiting both, fisheries and manufacturers. A proteomic approach, based on 2DE, tryptic in-gel digestion, MALDI-TOF MS, and ESI-MS/MS analyse...

journal_title：Journal of proteome research

authors： Ortea I,Cañas B,Gallardo JM

更新日期：2009-11-01 00:00:00

abstract：:Pathogenesis of Staphylococcus aureus, an opportunistic human pathogen, is complex and involves many virulence factors including an array of surface proteins (adhesins) that promote bacterial interactions with extracellular matrix components. A better understanding of these interactions can be achieved by studying the...

journal_title：Journal of proteome research

authors： Nandakumar R,Nandakumar MP,Marten MR,Ross JM

更新日期：2005-03-01 00:00:00

abstract：:Spectral libraries provide a sensitive and accurate method for identifying peptides from tandem mass spectra, complementary to searching genome-derived databases or sequencing de novo. Their application requires comprehensive libraries including peptides from low-abundant proteins. Here we describe a method for constr...

journal_title：Journal of proteome research

authors： van der Plas-Duivesteijn SJ,Mohammed Y,Dalebout H,Meijer A,Botermans A,Hoogendijk JL,Henneman AA,Deelder AM,Spaink HP,Palmblad M

更新日期：2014-03-07 00:00:00

abstract：:Direct tissue profiling and imaging mass spectrometry (MS) provides a detailed assessment of the complex protein pattern within a tissue sample. MALDI MS analysis of thin tissue sections results in over of 500 individual protein signals in the mass range of 2 to 70 kDa that directly correlate with protein composition ...

journal_title：Journal of proteome research

authors： Chaurand P,Schwartz SA,Caprioli RM

更新日期：2004-03-01 00:00:00

abstract：:14-3-3s are phosphoserine/phosphotreonine binding proteins that play pivotal roles as regulators of multiple cellular processes in eukaryotes. The flagellated protozoan parasite Giardia duodenalis, the causing agent of giardiasis, is a valuable simplified eukaryotic model. A single 14-3-3 isoform (g14-3-3) is expresse...

journal_title：Journal of proteome research

authors： Lalle M,Camerini S,Cecchetti S,Sayadi A,Crescenzi M,Pozio E

更新日期：2012-05-04 00:00:00

abstract：:Imatinib is the first molecular targeted therapy that has shown clinical success, but imatinib acquired resistance, although a rare event, is critical during the therapy of chronic myelogenous leukaemia (CML). With the aim of better understanding the molecular mechanisms accompanying acquisition of resistance to this ...

journal_title：Journal of proteome research

authors： Ferrari G,Pastorelli R,Buchi F,Spinelli E,Gozzini A,Bosi A,Santini V

更新日期：2007-01-01 00:00:00

abstract：:Chronic Obstructive Pulmonary Disease (COPD), a lung disease related to smoking, is one of the leading causes of chronic morbidity and mortality around the world. One goal in COPD research is the identification of biomarkers for early diagnosis of the disease. Here, we sought COPD-specific changes in the proteome from...

journal_title：Journal of proteome research

authors： Ohlmeier S,Vuolanto M,Toljamo T,Vuopala K,Salmenkivi K,Myllärniemi M,Kinnula VL

更新日期：2008-12-01 00:00:00

abstract：:The SEQUEST program was the first and remains one of the most widely used tools for assigning a peptide sequence within a database to a tandem mass spectrum. The cross correlation score is the primary score function implemented within SEQUEST and it is this score that makes the tool particularly sensitive. Unfortunate...

journal_title：Journal of proteome research

authors： Eng JK,Fischer B,Grossmann J,Maccoss MJ

更新日期：2008-10-01 00:00:00

abstract：:This study presents a comparative proteomic analysis of the membrane subproteome of whole Arabidopsis seedlings using 2% Brij-58 or 60% methanol to enrich and solubilize membrane proteins for strong cation exchange fractionation and reversed-phase liquid chromatography-tandem mass spectrometry (LC-MS/MS). A total of 4...

journal_title：Journal of proteome research

authors： Mitra SK,Gantt JA,Ruby JF,Clouse SD,Goshe MB

更新日期：2007-05-01 00:00:00

abstract：:The skin is the primary boundary between the body and the environment. In addition to its properties as a physical barrier, skin keratinocytes actively participate in many defense mechanisms. Viral double-stranded RNA (dsRNA) is the most important viral structure involved in activation of immune response. Intracellula...

journal_title：Journal of proteome research

authors： Ohman T,Lietzén N,Välimäki E,Melchjorsen J,Matikainen S,Nyman TA

更新日期：2010-03-05 00:00:00

abstract：:Analysis of the cerebrospinal fluid (CSF) proteome has proven valuable to the study of neurodegenerative disorders. To identify new protein/pathway alterations and candidate biomarkers for amyotrophic lateral sclerosis (ALS), we performed comparative proteomic profiling of CSF from sporadic ALS (sALS), healthy control...

journal_title：Journal of proteome research

authors： Collins MA,An J,Hood BL,Conrads TP,Bowser RP

更新日期：2015-11-06 00:00:00

abstract：:A popular method for peptide quantification relies on isobaric labeling such as tandem mass tags (TMT), which enables multiplexed proteome analyses. Quantification is achieved by reporter ions generated by fragmentation in a tandem mass spectrometer. However, with higher degrees of multiplexing, the smaller mass diffe...

journal_title：Journal of proteome research

authors： Kelstrup CD,Aizikov K,Batth TS,Kreutzman A,Grinfeld D,Lange O,Mourad D,Makarov AA,Olsen JV

更新日期：2018-11-02 00:00:00

abstract：:Protein cleavage coupled with isotope dilution mass spectrometry (PC-IDMS) has the potential to provide the absolute concentration of a specific protein, or multiple proteins, in complex mixtures. However, PC-IDMS differs from standard IDMS since the internal standard is a different molecule than the analyte at the st...

journal_title：Journal of proteome research

authors： Barnidge DR,Hall GD,Stocker JL,Muddiman DC

更新日期：2004-05-01 00:00:00

abstract：:In-depth, reproducible coverage of complex proteomes is challenging because the complexity of tryptic digests subjected to LC-MS/MS analysis frequently exceeds mass spectrometer analytical capacity, which results in undersampling of data. In this study, we used cancer cell lysates to systematically compare the commonl...

journal_title：Journal of proteome research

authors： Wang H,Chang-Wong T,Tang HY,Speicher DW

更新日期：2010-02-05 00:00:00

abstract：:MS-based de novo peptide sequencing has been improved remarkably with significant development of mass-spectrometry and computational approaches but still lacks quality-control methods. Here we proposed a novel algorithm pSite to evaluate the confidence of each amino acid rather than the full-length peptides obtained b...

journal_title：Journal of proteome research

authors： Yang H,Chi H,Zhou WJ,Zeng WF,Liu C,Wang RM,Wang ZW,Niu XN,Chen ZL,He SM

更新日期：2018-01-05 00:00:00

abstract：:Staphylococcus aureus with spa-type t437 has been identified as a predominant community-associated methicillin-resistant S. aureus clone from Asia, which is also encountered in Europe. Molecular typing has previously shown that t437 isolates are highly similar regardless of geographical regions or host environments. T...

journal_title：Journal of proteome research

authors： Zhao X,Palma Medina LM,Stobernack T,Glasner C,de Jong A,Utari P,Setroikromo R,Quax WJ,Otto A,Becher D,Buist G,van Dijl JM

更新日期：2019-07-05 00:00:00

abstract：:Stroke remains a major public health problem worldwide; it causes severe disability and is associated with high mortality rates. However, early diagnosis of stroke is difficult, and no reliable biomarkers are currently established. In this study, mass-spectrometry-based metabolomics was utilized to characterize the me...

journal_title：Journal of proteome research

authors： Liu P,Li R,Antonov AA,Wang L,Li W,Hua Y,Guo H,Wang L,Liu P,Chen L,Tian Y,Xu F,Zhang Z,Zhu Y,Huang Y

更新日期：2017-02-03 00:00:00

abstract：:Hypoxia inducible factor (HIF)-2α protein expression in solid tumors promotes stem-like phenotype in cancer stem cells and increases tumorigenic potential in nonstem cancer cells. Recently, we have shown that HIF-1/2α gene expression is correlated to neuroblastoma (NB) poor survival and to undifferentiated tumor state...

journal_title：Journal of proteome research

authors： Cimmino F,Pezone L,Avitabile M,Persano L,Vitale M,Sassi M,Bresolin S,Serafin V,Zambrano N,Scaloni A,Basso G,Iolascon A,Capasso M

更新日期：2016-10-07 00:00:00

abstract：:Secreted proteins play a key role in cell signaling, communication, and migration. We recently described the development of an aggressive variant (T24M) of the bladder cancer cell line T24. Using this cell line model, the objective of our work was the identification of secreted proteins involved in the acquisition of ...

journal_title：Journal of proteome research

authors： Makridakis M,Roubelakis MG,Bitsika V,Dimuccio V,Samiotaki M,Kossida S,Panayotou G,Coleman J,Candiano G,Anagnou NP,Vlahou A

更新日期：2010-06-04 00:00:00

abstract：:12-O-Tetradecanoyl-phorbol-13-acetate (TPA) is a plant derivative with multiple function as tumor promoter, differentiation revulsant or leukemia therapy drug. The molecular mechanism of its function is perplexing. Many studies have focused on the mechanism of TPA stimulation in tumor promotion of mouse models or term...

journal_title：Journal of proteome research

authors： Jiang P,Gan M,Huang H,Shen X,Wang S,Yao K

更新日期：2005-03-01 00:00:00