Abstract:
:Drug resistance is one of the major causes of cancer chemotherapy failure. In the current study, we used a pair of lung adenocarcinoma cell lines, A549 and the pemetrexed-resistant A549/PEM cells, as a model to monitor resistance-dependent cellular responses and identify potential therapeutic targets. By means of 2D differential gel electrophoresis (2D-DIGE) and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS), we investigated the global protein expression alterations induced by pemetrexed treatment and resistance. The proteomic result revealed that pemetrexed exposure obviously altered the expression of 81 proteins in the A549 cells, whereas no significant response was observed in the similarly treated A549/PEM cells, hence implying an association between these proteins and the drug-specific response. Moreover, 72 proteins including flavin reductase and calreticulin demonstrated differential expression between the A549 and A549/PEM cells, indicating baseline resistance. Additional tests employed siRNA silencing, protein overexpression, cell viability analysis, and analysis of apoptosis to examine and confirm the potency of flavin reductase and calreticulin proteins in the development of pemetrexed resistance. In summary, by using a proteomic approach, we identified numerous proteins, including flavin reductase and calreticulin, involved in pemetrexed drug resistance-developing mechanisms. Our results provide useful diagnostic markers and therapeutic candidates for pemetrexed-resistant lung cancer treatment.
journal_name
J Proteome Resjournal_title
Journal of proteome researchauthors
Chou HC,Chen JY,Lin DY,Wen YF,Lin CC,Lin SH,Lin CH,Chung TW,Liao EC,Chen YJ,Wei YS,Tsai YT,Chan HLdoi
10.1021/acs.jproteome.5b00794subject
Has Abstractpub_date
2015-11-06 00:00:00pages
4907-20issue
11eissn
1535-3893issn
1535-3907journal_volume
14pub_type
杂志文章abstract::The paper describes the implementation of a software system based on the Fenyö disulfide bond assignment algorithm. The system allows an investigator to enter data derived from mass spectrum peak assignments, a target protein sequence and other experimental conditions. The output of the system is the set of disulfide ...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr034016a
更新日期:2003-11-01 00:00:00
abstract::Rheumatoid arthritis is a progressive, highly debilitating disease where early diagnosis, enabling rapid clinical intervention, would provide obvious benefits to patients, healthcare systems, and society. Novel biomarkers that enable noninvasive early diagnosis of the onset and progression of the disease provide one r...
journal_title:Journal of proteome research
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更新日期:2016-12-02 00:00:00
abstract::As a potential tool for proteomics and protein characterization, in-gel cysteine- and arginine-specific cleavage is demonstrated by means of trypsin or endoproteinase Lys-C for six model proteins (lysozyme, alpha-lactalbumin, beta-lactoglobulin, ribonuclease A, albumin, and transferrin), ranging in size from 14 kDa to...
journal_title:Journal of proteome research
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journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr8008572
更新日期:2009-03-01 00:00:00
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journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr101082c
更新日期:2011-05-06 00:00:00
abstract::Elevated atmospheric CO2 and nitrogen are major environmental factors affecting shoot growth. The objectives of this study are to determine the interactive effects of elevated CO2 and nitrogen on leaf growth in tall fescue ( Festuca arundinacea) and to identify major proteins and associated metabolic pathways underlyi...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/acs.jproteome.8b00951
更新日期:2019-06-07 00:00:00
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journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/acs.jproteome.7b00330
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journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr8000282
更新日期:2008-06-01 00:00:00
abstract::Human milk oligosaccharides (HMOs) perform a number of functions including serving as prebiotics to stimulate the growth of beneficial intestinal bacteria, as receptor analogues to inhibit binding of pathogens, and as substances that promote postnatal brain development. There is further evidence that HMOs participate ...
journal_title:Journal of proteome research
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更新日期:2010-08-06 00:00:00
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journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr3006233
更新日期:2012-09-07 00:00:00
abstract::The Baculoviral IAP repeat-containing protein 5 (BIRC5), also known as inhibitor of apoptosis protein survivin, is a member of the chromosomal passenger complex and a key player in mitosis. To investigate the function of BIRC5 in liver regeneration, we analyzed a hepatocyte-specific BIRC5-knockout mouse model using a ...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr401188r
更新日期:2014-06-06 00:00:00
abstract::Epithelial cells play an important role in physiological and pathophysiological situations, with organ-, tissue-, type-, and function-specific patterns. Proteome analysis has been used to study epithelial-origin diseases and identify novel prognostic, diagnostic, and therapeutic markers. The present review compares th...
journal_title:Journal of proteome research
pub_type: 杂志文章,评审
doi:10.1021/pr050389v
更新日期:2006-04-01 00:00:00
abstract::Protein cleavage coupled with isotope dilution mass spectrometry (PC-IDMS) has the potential to provide the absolute concentration of a specific protein, or multiple proteins, in complex mixtures. However, PC-IDMS differs from standard IDMS since the internal standard is a different molecule than the analyte at the st...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr034124x
更新日期:2004-05-01 00:00:00
abstract::During vertebrate central nervous system development, the apical neuroepithelium is bathed with embryonic Cerebrospinal Fluid (e-CSF) which plays regulatory roles in cortical cell proliferation and maintenance. Here, we report the first proteomic analysis of human e-CSF and compare it to an extensive proteomic analysi...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr070247w
更新日期:2007-09-01 00:00:00
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journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr401019d
更新日期:2014-06-06 00:00:00
abstract::Near-isogenic sunflower lines containing 25% (inbred RHA280) and 48% (RHA801) oil by seed dry mass were comparatively analyzed in biological triplicate at 18 days after flowering using two-dimensional (both pI 3-10 and 4-7) Difference Gel Electrophoresis. Additionally, two inbred lines varying in oleic acid content, H...
journal_title:Journal of proteome research
pub_type: 杂志文章
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journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr100789v
更新日期:2010-12-03 00:00:00
abstract::The effects of increased intake of dietary fiber as either whole grain rye or as resistant starch compared to a typical low dietary fiber Western-style diet on the metabolomics responses were studied in gastrointestinal content and tissue, peripheral plasma, and urine using a multicompartmental nontargeted liquid chro...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/acs.jproteome.5b00039
更新日期:2015-08-07 00:00:00
abstract::Lysine methylation plays important roles in structural and functional regulation of chromatin. Although trypsin is the most widely used protease in mass spectrometry-based proteomic analysis for lysine methylation substrates, the proteolytic activity of trypsin on dimethylated lysine residues remains an arguable issue...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/acs.jproteome.7b00373
更新日期:2017-09-01 00:00:00
abstract::Proteoforms containing post-translational modifications (PTMs) represent a degree of functional diversity only harnessed through analytically precise simultaneous quantification of multiple PTMs. Here we present a method to accurately differentiate an unmodified peptide from its PTM-containing counterpart through data...
journal_title:Journal of proteome research
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journal_title:Journal of proteome research
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journal_title:Journal of proteome research
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journal_title:Journal of proteome research
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journal_title:Journal of proteome research
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abstract::Manassantin A is a natural product that has been shown to have anticancer activity in cell-based assays, but has a largely unknown mode-of-action. Described here is the use of two different energetics-based approaches to identify protein targets of manassantin A. Using the stability of proteins from rates of oxidation...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/acs.jproteome.6b00237
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journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr300893w
更新日期:2013-01-04 00:00:00
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journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/acs.jproteome.9b00615
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journal_title:Journal of proteome research
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doi:10.1021/pr300273g
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journal_title:Journal of proteome research
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journal_title:Journal of proteome research
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