当前位置： SCI文献检索 > JOURNAL OF PROTEOME RESEARCH期刊下所有文献 > Vegetative Cell and Spore Proteomes of Clostridioides difficile Show Finite Differences and Reveal Potential Protein Markers.

Vegetative Cell and Spore Proteomes of Clostridioides difficile Show Finite Differences and Reveal Potential Protein Markers.

Abstract：

:Clostridioides difficile-associated infection (CDI) is a health-care-associated infection caused, as the name suggests, by obligate anaerobic pathogen C. difficile and thus mainly transmitted via highly resistant endospores from one person to the other. In vivo, the spores need to germinate into cells prior to establishing an infection. Bile acids and glycine, both available in sufficient amounts inside the human host intestinal tract, serve as efficient germinants for the spores. It is therefore, for better understanding of C. difficile virulence, crucial to study both the cell and spore states with respect to their genetic, metabolic, and proteomic composition. In the present study, mass spectrometric relative protein quantification, based on the 14N/15N peptide isotopic ratios, has led to quantification of over 700 proteins from combined spore and cell samples. The analysis has revealed that the proteome turnover between a vegetative cell and a spore for this organism is moderate. Additionally, specific cell and spore surface proteins, vegetative cell proteins CD1228, CD3301 and spore proteins CD2487, CD2434, and CD0684 are identified as potential protein markers for C. difficile infection.

journal_name

J Proteome Res

journal_title

Journal of proteome research

authors

Abhyankar WR,Zheng L,Brul S,de Koster CG,de Koning LJ

doi

10.1021/acs.jproteome.9b00413

subject

Has Abstract

pub_date

2019-11-01 00:00:00

pages

3967-3976

issue

11

eissn

1535-3893

issn

1535-3907

journal_volume

18

pub_type

杂志文章

相关文献

JOURNAL OF PROTEOME RESEARCH文献大全
  • Untargeted metabolomic profiling of amphenicol-resistant Campylobacter jejuni by ultra-high-performance liquid chromatography-mass spectrometry.

    abstract：:Campylobacter jejuni, an important foodborne microorganism, poses severe and emergent threats to human health as antibiotic resistance becomes increasingly prevalent. The mechanisms of drug resistance are hard to decipher, and little is known at the metabolic level. Here we apply metabolomic profiling to discover meta...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/pr501061d

    authors： Li H,Xia X,Li X,Naren G,Fu Q,Wang Y,Wu C,Ding S,Zhang S,Jiang H,Li J,Shen J

    更新日期：2015-02-06 00:00:00

  • Identification of novel proteins from the venom of a cryptic snake Drysdalia coronoides by a combined transcriptomics and proteomics approach.

    abstract：:We have investigated the transcriptome and proteome of the venom of a cryptic Australian elapid snake Drysdalia coronoides. To probe into the transcriptome, we constructed a partial cDNA library from the venom gland of D. coronoides. The proteome of the venom of D. coronoides was explored by tryptic digestion of the c...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/pr1008916

    authors： Chatrath ST,Chapeaurouge A,Lin Q,Lim TK,Dunstan N,Mirtschin P,Kumar PP,Kini RM

    更新日期：2011-02-04 00:00:00

  • Construction and Deciphering of Human Phosphorylation-Mediated Signaling Transduction Networks.

    abstract：:Protein phosphorylation is the most abundant reversible covalent modification. Human protein kinases participate in almost all biological pathways, and approximately half of the kinases are associated with disease. PhoSigNet was designed to store and display human phosphorylation-mediated signal transduction networks,...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/acs.jproteome.5b00249

    authors： Zhang M,Li H,He Y,Sun H,Xia L,Wang L,Sun B,Ma L,Zhang G,Li J,Li Y,Xie L

    更新日期：2015-07-02 00:00:00

  • Sonoreactor-based technology for fast high-throughput proteolytic digestion of proteins.

    abstract：:Fast (120 s) and high-throughput (more than six samples at once) in-gel trypsin digestion of proteins using sonoreactor technology has been achieved. Successful protein identification was done by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, MALDI-TOF-MS. Specific identification of the ...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/pr060508m

    authors： Rial-Otero R,Carreira RJ,Cordeiro FM,Moro AJ,Fernandes L,Moura I,Capelo JL

    更新日期：2007-02-01 00:00:00

  • Integrative Omics Analysis Reveals Post-Transcriptionally Enhanced Protective Host Response in Colorectal Cancers with Microsatellite Instability.

    abstract：:Microsatellite instability (MSI) is a frequent and clinically relevant molecular phenotype in colorectal cancer. MSI cancers have favorable survival compared with microsatellite stable cancers (MSS), possibly due to the pronounced tumor-infiltrating lymphocytes observed in MSI cancers. Consistent with the strong immun...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/acs.jproteome.5b00847

    authors： Liu Q,Zhang B

    更新日期：2016-03-04 00:00:00

  • Porphyromonas gingivalis Gingipains Display Transpeptidation Activity.

    abstract：:Porphyromonas gingivalis is a keystone periodontal pathogen that has been associated with autoimmune disorders. The cell surface proteases Lys-gingipain (Kgp) and Arg-gingipains (RgpA and RgpB) are major virulence factors, and their proteolytic activity is enhanced by small peptides such as glycylglycine (GlyGly). The...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/acs.jproteome.8b00286

    authors： Zhang L,Veith PD,Huq NL,Chen YY,Seers CA,Cross KJ,Gorasia DG,Reynolds EC

    更新日期：2018-08-03 00:00:00

  • IDAWG: Metabolic incorporation of stable isotope labels for quantitative glycomics of cultured cells.

    abstract：:Robust quantification is an essential component of comparative -omic strategies. In this regard, glycomics lags behind proteomics. Although various isotope-tagging and direct quantification methods have recently enhanced comparative glycan analysis, a cell culture labeling strategy, that could provide for glycomics th...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/pr8010028

    authors： Orlando R,Lim JM,Atwood JA 3rd,Angel PM,Fang M,Aoki K,Alvarez-Manilla G,Moremen KW,York WS,Tiemeyer M,Pierce M,Dalton S,Wells L

    更新日期：2009-08-01 00:00:00

  • Analysis of secreted proteins for the study of bladder cancer cell aggressiveness.

    abstract：:Secreted proteins play a key role in cell signaling, communication, and migration. We recently described the development of an aggressive variant (T24M) of the bladder cancer cell line T24. Using this cell line model, the objective of our work was the identification of secreted proteins involved in the acquisition of ...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/pr100189d

    authors： Makridakis M,Roubelakis MG,Bitsika V,Dimuccio V,Samiotaki M,Kossida S,Panayotou G,Coleman J,Candiano G,Anagnou NP,Vlahou A

    更新日期：2010-06-04 00:00:00

  • Proteomics, genomics, and pathway analyses of Escherichia coli and Staphylococcus aureus infected milk whey reveal molecular pathways and networks involved in mastitis.

    abstract：:Gram-negative and -positive bacteria elicit different response patterns by the host. The proteomic profiles of milk whey samples from cows naturally infected with Escherichia coli or Staphyloccocus aureus as compared to whey from healthy cows were determined by one-dimensional, liquid chromatography-tandem mass spectr...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/pr100336e

    authors： Ibeagha-Awemu EM,Ibeagha AE,Messier S,Zhao X

    更新日期：2010-09-03 00:00:00

  • Advances in Higher Order Multiplexing Techniques in Proteomics.

    abstract：:Proteomics by mass spectrometry (MS) allows the large-scale identification and quantitation of the cellular proteins in a given biological context. Systems biology studies from proteomics data are largely limited by the accuracy and coverage of quantitative proteomics along with missing values. Toward this end, statis...

    journal_title：Journal of proteome research

    pub_type： 杂志文章,评审

    doi：10.1021/acs.jproteome.9b00228

    authors： Aggarwal S,Talukdar NC,Yadav AK

    更新日期：2019-06-07 00:00:00

  • Site-specific quantitative analysis of overglycosylation of collagen in osteogenesis imperfecta using hydrazide chemistry and SILAC.

    abstract：:We recently developed a novel method for analysis of collagen O-glycosylations, which include galactosyl-hydroxylysine (GHL) and glucosyl-galactosyl-hydroxylysine (GGHL), using hydrazide chemistry (Taga, Y., Mol. Cell. Proteomics 2012, 11 (6), M111.010397). Here we investigated an overglycosylation model of collagen p...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/pr400079d

    authors： Taga Y,Kusubata M,Ogawa-Goto K,Hattori S

    更新日期：2013-05-03 00:00:00

  • Relative quantification of membrane proteins in wild-type and prion protein (PrP)-knockout cerebellar granule neurons.

    abstract：:Approximately 25% of eukaryotic proteins possessing homology to at least two transmembrane domains are predicted to be embedded in biological membranes. Nevertheless, this group of proteins is not usually well represented in proteome-wide experiments due to their refractory nature. Here we present a quantitative mass ...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/pr200759m

    authors： Stella R,Cifani P,Peggion C,Hansson K,Lazzari C,Bendz M,Levander F,Sorgato MC,Bertoli A,James P

    更新日期：2012-02-03 00:00:00

  • Proteomic analysis of protein expression affected by peroxiredoxin V knock-down in hypoxic kidney.

    abstract：:Peroxiredoxin V, an atypical thioredoxin peroxidase, is widely expressed in mammalian tissues. In addition, Prdx V is localized in mitochondria, peroxisome, cytosol, and the nucleus. Prdx V has been reported to protect a wide range of cellular environments as an antioxidant enzyme, and its dysfunctions may be implicat...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/pr100190b

    authors： Yang HY,Kwon J,Cho EJ,Choi HI,Park C,Park HR,Park SH,Chung KJ,Ryoo ZY,Cho KO,Lee TH

    更新日期：2010-08-06 00:00:00

  • Sputum proteomics identifies elevated PIGR levels in smokers and mild-to-moderate COPD.

    abstract：:Chronic obstructive pulmonary disease (COPD) is one of the leading causes of morbidity and mortality around the world. However, the exact mechanisms leading to COPD and its progression are still poorly understood. In this study, induced sputum was analyzed by cysteine-specific two-dimensional difference gel electropho...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/pr2006395

    authors： Ohlmeier S,Mazur W,Linja-Aho A,Louhelainen N,Rönty M,Toljamo T,Bergmann U,Kinnula VL

    更新日期：2012-02-03 00:00:00

  • Proteomic Analysis of Brain and Cerebrospinal Fluid from the Three Major Forms of Neuronal Ceroid Lipofuscinosis Reveals Potential Biomarkers.

    abstract：:Clinical trials have been conducted for the neuronal ceroid lipofuscinoses (NCLs), a group of neurodegenerative lysosomal diseases that primarily affect children. Whereas clinical rating systems will evaluate long-term efficacy, biomarkers to measure short-term response to treatment would be extremely valuable. To ide...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/acs.jproteome.7b00460

    authors： Sleat DE,Tannous A,Sohar I,Wiseman JA,Zheng H,Qian M,Zhao C,Xin W,Barone R,Sims KB,Moore DF,Lobel P

    更新日期：2017-10-06 00:00:00

  • Cellular responses associated with ROS production and cell fate decision in early stress response to iron limitation in the diatom Thalassiosira pseudonana.

    abstract：:Investigation of how diatoms cope with the rapid fluctuations in iron bioavailability in marine environments may facilitate a better understanding of the mechanisms underlying their ecological success, in particular their ability to proliferate rapidly during favorable conditions. In this study, using in vivo biochemi...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/pr5004664

    authors： Luo CS,Liang JR,Lin Q,Li C,Bowler C,Anderson DM,Wang P,Wang XW,Gao YH

    更新日期：2014-12-05 00:00:00

  • DeNovoGUI: an open source graphical user interface for de novo sequencing of tandem mass spectra.

    abstract：:De novo sequencing is a popular technique in proteomics for identifying peptides from tandem mass spectra without having to rely on a protein sequence database. Despite the strong potential of de novo sequencing algorithms, their adoption threshold remains quite high. We here present a user-friendly and lightweight gr...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/pr4008078

    authors： Muth T,Weilnböck L,Rapp E,Huber CG,Martens L,Vaudel M,Barsnes H

    更新日期：2014-02-07 00:00:00

  • Characterization of amyloid beta peptides in cerebrospinal fluid by an automated immunoprecipitation procedure followed by mass spectrometry.

    abstract：:Pathogenic events in Alzheimer's disease are believed to involve an imbalance between the production and clearance of the neurotoxic 42 amino acid form of the beta-amyloid peptide (Abeta1-42). Although much is known about the production of Abeta1-42, many questions remain about its degradation. Here, we describe an op...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/pr0703627

    authors： Portelius E,Tran AJ,Andreasson U,Persson R,Brinkmalm G,Zetterberg H,Blennow K,Westman-Brinkmalm A

    更新日期：2007-11-01 00:00:00

  • Proteomic Profiling of Fast Neutron-Induced Soybean Mutant Unveiled Pathways Associated with Increased Seed Protein Content.

    abstract：:Mutagenesis through fast neutron (FN) radiation of soybean resulted in a mutant with a 15% increase in seed protein content. A comparative genomic hybridization analysis confirmed that the mutant is lacking 24 genes located at chromosomes 5 and 10. A tandem mass tag-based proteomic profiling of the wild type and the F...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/acs.jproteome.0c00160

    authors： Islam N,Krishnan HB,Natarajan S

    更新日期：2020-10-02 00:00:00

  • In-gel derivatization of proteins for cysteine-specific cleavages and their analysis by mass spectrometry.

    abstract：:As a potential tool for proteomics and protein characterization, in-gel cysteine- and arginine-specific cleavage is demonstrated by means of trypsin or endoproteinase Lys-C for six model proteins (lysozyme, alpha-lactalbumin, beta-lactoglobulin, ribonuclease A, albumin, and transferrin), ranging in size from 14 kDa to...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/pr025568g

    authors： Thevis M,Ogorzalek Loo RR,Loo JA

    更新日期：2003-03-01 00:00:00

  • Label-free proteomics and systems biology analysis of mycobacterial phagosomes in dendritic cells and macrophages.

    abstract：:Proteomics has been applied to study intracellular bacteria and phagocytic vacuoles in different host cell lines, especially macrophages (Mφs). For mycobacterial phagosomes, few studies have identified over several hundred proteins for systems assessment of the phagosome maturation and antigen presentation pathways. M...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/pr101245u

    authors： Li Q,Singh CR,Ma S,Price ND,Jagannath C

    更新日期：2011-05-06 00:00:00

  • A sulfated, phosphorylated 7 kDa secreted peptide characterized by direct analysis of cell culture media.

    abstract：:An unusual sulfotyrosine-, phosphoserine-containing motif was mapped on a differentially post-translationally modified 60 residue antimicrobial neuroendocrine peptide called chrombacin. The study was performed by high resolution FT MS using complementary fragmentation techniques. The peptide was analyzed at low levels...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/pr7006686

    authors： Taylor SW,Sun C,Hsieh A,Andon NL,Ghosh SS

    更新日期：2008-02-01 00:00:00

  • iTRAQ-based quantitative proteomics of developing and ripening muscadine grape berry.

    abstract：:Grapes are among the widely cultivated fruit crops in the world. Grape berries like other nonclimacteric fruits undergo a complex set of dynamic, physical, physiological, and biochemical changes during ripening. Muscadine grapes are widely cultivated in the southern United States for fresh fruit and wine. To date, cha...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/pr400731p

    authors： Kambiranda D,Katam R,Basha SM,Siebert S

    更新日期：2014-02-07 00:00:00

  • Analysis of oxygen/glucose-deprivation-induced changes in SUMO3 conjugation using SILAC-based quantitative proteomics.

    abstract：:Transient cerebral ischemia dramatically activates small ubiquitin-like modifier (SUMO2/3) conjugation. In cells exposed to 6 h of transient oxygen/glucose deprivation (OGD), a model of ischemia, SUMOylation increases profoundly between 0 and 30 min following re-oxygenation. To elucidate the effect of transient OGD on...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/pr200834f

    authors： Yang W,Thompson JW,Wang Z,Wang L,Sheng H,Foster MW,Moseley MA,Paschen W

    更新日期：2012-02-03 00:00:00

  • Predictive metabolomics evaluation of nutrition-modulated metabolic stress responses in human blood serum during the early recovery phase of strenuous physical exercise.

    abstract：:We have investigated whether postexercise ingestion of carbohydrates in combination with proteins generates a different systemic metabolic response, as compared to the sole ingestion of carbohydrate or water, in the early recovery phase following exercise. In addition, metabolic patterns related to fitness level were ...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/pr900081q

    authors： Chorell E,Moritz T,Branth S,Antti H,Svensson MB

    更新日期：2009-06-01 00:00:00

  • IR-MALDI-LDI combined with ion mobility orthogonal time-of-flight mass spectrometry.

    abstract：:Most MALDI instrumentation uses UV lasers. We have designed a MALDI-IM-oTOF-MS which employs both a Nd:YAG laser pumped optical parametric oscillator (OPOTEK, lambda = 2.8-3.2 microm at 20 Hz) to perform IR-LDI or IR-MALDI and a Nd:YLF laser (Crystalaser, lambda = 249 nm at 200 Hz) for the UV. Ion mobility (IM) gives ...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/pr060055l

    authors： Woods AS,Ugarov M,Jackson SN,Egan T,Wang HY,Murray KK,Schultz JA

    更新日期：2006-06-01 00:00:00

  • Quantitative overview of N2 fixation in Nostoc punctiforme ATCC 29133 through cellular enrichments and iTRAQ shotgun proteomics.

    abstract：:Nostoc punctiforme ATCC 29133 is a photoautotrophic cyanobacterium with the capacity to fix atmospheric N 2. Its ability to mediate this process is similar to that described for Nostoc sp. PCC 7120, where vegetative cells differentiate into heterocysts. Quantitative proteomic investigations at both the filament level ...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/pr800285v

    authors： Ow SY,Noirel J,Cardona T,Taton A,Lindblad P,Stensjö K,Wright PC

    更新日期：2009-01-01 00:00:00

  • Modulation of colon cancer by nutmeg.

    abstract：:Colon cancer is the most common cancer and the third leading cause of cancer mortality in humans. Using mass spectrometry-based metabolomics, the current study revealed the accumulation of four uremic toxins (cresol sulfate, cresol glucuronide, indoxyl sulfate, and phenyl sulfate) in the serum of mice harboring adenom...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/pr5013152

    authors： Li F,Yang XW,Krausz KW,Nichols RG,Xu W,Patterson AD,Gonzalez FJ

    更新日期：2015-04-03 00:00:00

  • In-gel isoelectric focusing of peptides as a tool for improved protein identification.

    abstract：:In the analysis of proteins in complex samples, pre-fractionation is imperative to obtain the necessary depth in the number of reliable protein identifications by mass spectrometry. Here we explore isoelectric focusing of peptides (peptide IEF) as an effective fractionation step that at the same time provides the adde...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/pr0601180

    authors： Krijgsveld J,Gauci S,Dormeyer W,Heck AJ

    更新日期：2006-07-01 00:00:00

  • Proteome analysis of silkworm, Bombyx mori, larval gonads: characterization of proteins involved in sexual dimorphism and gametogenesis.

    abstract：:Sexual dimorphism is initialed by the components of the sex determination pathway and is most evident in gonads and germ cells. Although striking dimorphic expressions have been detected at the transcriptional level between the silkworm larval testis and the ovary, the sex-dimorphic expressions at the protein level ha...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/pr300920z

    authors： Chen JE,Li JY,You ZY,Liu LL,Liang JS,Ma YY,Chen M,Zhang HR,Jiang ZD,Zhong BX

    更新日期：2013-06-07 00:00:00