Vegetative Cell and Spore Proteomes of Clostridioides difficile Show Finite Differences and Reveal Potential Protein Markers.


:Clostridioides difficile-associated infection (CDI) is a health-care-associated infection caused, as the name suggests, by obligate anaerobic pathogen C. difficile and thus mainly transmitted via highly resistant endospores from one person to the other. In vivo, the spores need to germinate into cells prior to establishing an infection. Bile acids and glycine, both available in sufficient amounts inside the human host intestinal tract, serve as efficient germinants for the spores. It is therefore, for better understanding of C. difficile virulence, crucial to study both the cell and spore states with respect to their genetic, metabolic, and proteomic composition. In the present study, mass spectrometric relative protein quantification, based on the 14N/15N peptide isotopic ratios, has led to quantification of over 700 proteins from combined spore and cell samples. The analysis has revealed that the proteome turnover between a vegetative cell and a spore for this organism is moderate. Additionally, specific cell and spore surface proteins, vegetative cell proteins CD1228, CD3301 and spore proteins CD2487, CD2434, and CD0684 are identified as potential protein markers for C. difficile infection.


J Proteome Res


Abhyankar WR,Zheng L,Brul S,de Koster CG,de Koning LJ




Has Abstract


2019-11-01 00:00:00












  • Identification of ethylene-mediated protein changes during nodulation in Medicago truncatula using proteome analysis.

    abstract::Ethylene has been hypothesised to be a regulator of root nodule development in legumes, but its molecular mechanisms of action remain unclear. The skl mutant is an ethylene-insensitive legume mutant showing a hypernodulation phenotype when inoculated with its symbiont Sinorhizobium meliloti. We used the skl mutant to ...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Prayitno J,Imin N,Rolfe BG,Mathesius U

    更新日期:2006-11-01 00:00:00

  • On the development of plasma protein biomarkers.

    abstract::The development of plasma biomarkers has proven to be more challenging than initially anticipated. Many studies have reported lists of candidate proteins rather than validated candidate markers with an assigned performance to a specific clinical objective. Biomarker research necessitates a clear rational framework wit...

    journal_title:Journal of proteome research

    pub_type: 杂志文章,评审


    authors: Surinova S,Schiess R,Hüttenhain R,Cerciello F,Wollscheid B,Aebersold R

    更新日期:2011-01-07 00:00:00

  • Global Analysis of Protein Lysine Succinylation Profiles and Their Overlap with Lysine Acetylation in the Marine Bacterium Vibrio parahemolyticus.

    abstract::Protein lysine acylation, including acetylation and succinylation, has been found to be a major post-translational modification (PTM) and is associated with the regulation of cellular processes that are widespread in bacteria. Vibrio parahemolyticus is a model marine bacterium that causes seafood-borne illness in huma...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Pan J,Chen R,Li C,Li W,Ye Z

    更新日期:2015-10-02 00:00:00

  • abFASP-MS: affinity-based filter-aided sample preparation mass spectrometry for quantitative analysis of chemically labeled protein complexes.

    abstract::Affinity purification coupled to 1-D gel-free liquid chromatography mass spectrometry (LC-MS) is a well-established and widespread approach for the analyses of noncovalently interacting protein complexes. In this study, two proteins conjugated to a streptavidin-binding peptide and hemagglutinin double tag were express...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Huber ML,Sacco R,Parapatics K,Skucha A,Khamina K,Müller AC,Rudashevskaya EL,Bennett KL

    更新日期:2014-02-07 00:00:00

  • NMRmix: A Tool for the Optimization of Compound Mixtures in 1D (1)H NMR Ligand Affinity Screens.

    abstract::NMR ligand affinity screening is a powerful technique that is routinely used in drug discovery or functional genomics to directly detect protein-ligand binding events. Binding events can be identified by monitoring differences in the 1D (1)H NMR spectrum of a compound with and without protein. Although a single NMR sp...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Stark JL,Eghbalnia HR,Lee W,Westler WM,Markley JL

    更新日期:2016-04-01 00:00:00

  • Citrate boosts the performance of phosphopeptide analysis by UPLC-ESI-MS/MS.

    abstract::Incomplete recovery from the LC column is identified as a major cause for poor detection efficiency of phosphopeptides by LC-MS/MS. It is proposed that metal ions adsorbed on the stationary phase interact with the phosphate group of phosphopeptides via an ion-pairing mechanism related to IMAC (IMAC: immobilized metal ...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Winter D,Seidler J,Ziv Y,Shiloh Y,Lehmann WD

    更新日期:2009-01-01 00:00:00

  • 2018 YPIC Challenge: A Case Study in Characterizing an Unknown Protein Sample.

    abstract::For the 2018 YPIC Challenge, contestants were invited to try to decipher two unknown English questions encoded by a synthetic protein expressed in Escherichia coli. In addition to deciphering the sentence, contestants were asked to determine the three-dimensional structure and detect any post-translation modifications...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Pino L,Lin A,Bittremieux W

    更新日期:2019-11-01 00:00:00

  • Glycoproteomic analysis of glioblastoma stem cell differentiation.

    abstract::Cancer stem cells are responsible for tumor formation through self-renewal and differentiation into multiple cell types and thus represent a new therapeutic target for tumors. Glycoproteins play a critical role in determining the fates of stem cells such as self-renewal, proliferation, and differentiation. Here we app...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: He J,Liu Y,Zhu TS,Xie X,Costello MA,Talsma CE,Flack CG,Crowley JG,Dimeco F,Vescovi AL,Fan X,Lubman DM

    更新日期:2011-01-07 00:00:00

  • On-line concentration of microheterogeneous proteins by capillary electrophoresis using SDS and PEO as additives.

    abstract::In this paper, we describe a method for analyzing large-volume protein samples using capillary electrophoresis in conjunction with laser-induced fluorescence detection (CE-LIF). To improve the stacking and separation efficiencies of proteins, we added either 0.01% sodium dodecyl sulfate (SDS) or 0.01% poly(ethylene ox...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Huang YF,Hsieh MM,Tseng WL,Chang HT

    更新日期:2006-02-01 00:00:00

  • Proteomic analysis of multiprotein complexes in the thylakoid membrane upon cadmium treatment.

    abstract::The time course of the thylakoid membranes proteomic profile changes upon cadmium (Cd) addition to hydroponic Spinacia oleracea L. plants has been investigated. Two different proteomic approaches have been used: blue native gel electrophoresis followed by SDS-PAGE (2D BN-SDS-PAGE) and sucrose density gradient ultracen...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Fagioni M,D'Amici GM,Timperio AM,Zolla L

    更新日期:2009-01-01 00:00:00

  • Interaction of Drug Candidates with Various SARS-CoV-2 Receptors: An in Silico Study to Combat COVID-19.

    abstract::The world is currently facing the COVID-19 pandemic caused by the SARS-CoV-2 virus. The pandemic is causing the death of people around the world, and public and social health measures to slow or prevent the spread of COVID-19 are being implemented with the involvement of all members of society. Research institutions a...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Barros RO,Junior FLCC,Pereira WS,Oliveira NMN,Ramos RM

    更新日期:2020-11-06 00:00:00

  • Subtractive proteomic approach to the endometrial carcinoma invasion front.

    abstract::Tumor invasion defines the transition between tissue-restricted carcinomas, related to good outcome as optimal surgery becomes possible, and metastatic tumors associated with poor prognosis and a dramatic decrease in survival. In endometrial cancer, myometrial infiltration represents a determinant parameter highly val...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Monge M,Doll A,Colas E,Gil-Moreno A,Castellvi J,Garcia A,Colome N,Perez-Benavente A,Pedrola N,Lopez-Lopez R,Dolcet X,Ramon y Cajal S,Xercavins J,Matias-Guiu X,Canals F,Reventos J,Abal M

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  • A Novel Proteomics-Based Strategy for the Investigation of Peptide Sequences in Extraterrestrial Samples.

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    journal_title:Journal of proteome research

    pub_type: 杂志文章


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  • Primary cell cultures arising from normal kidney and renal cell carcinoma retain the proteomic profile of corresponding tissues.

    abstract::Renal cell carcinoma (RCC) tissue is composed of a mixture of neoplastic and normal cells, which complicate proteome analysis. The aim of our study was to investigate whether it is feasible to establish primary cell cultures of RCC and of renal cortex maintaining the tissue phenotype along with a more homogeneous and ...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


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    更新日期:2005-09-01 00:00:00

  • Worker honeybee sterility: a proteomic analysis of suppressed ovary activation.

    abstract::Eusocial behavior is extensively studied in the honeybee, Apis mellifera, as it displays an extreme form of altruism. Honeybee workers are generally obligatory sterile in a bee colony headed by a queen, but the inhibition of ovary activation is lifted upon the absence of queen and larvae. Worker bees are then able to ...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Cardoen D,Ernst UR,Boerjan B,Bogaerts A,Formesyn E,de Graaf DC,Wenseleers T,Schoofs L,Verleyen P

    更新日期:2012-05-04 00:00:00

  • Quantitative analysis of brain nuclear phosphoproteins identifies developmentally regulated phosphorylation events.

    abstract::Protein phosphorylation is a globally adopted and tightly controlled post-translational modification, and represents one of the most important molecular switching mechanisms that govern the entire spectrum of biological processes. In the central nervous system, it has been demonstrated that phosphorylation of key prot...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Liao L,McClatchy DB,Park SK,Xu T,Lu B,Yates JR 3rd

    更新日期:2008-11-01 00:00:00

  • Selenite-induced toxicity in cancer cells is mediated by metabolic generation of endogenous selenium nanoparticles.

    abstract::Selenite has been a touted cancer chemopreventative agent but generates conflicting outcomes. Multiple mechanisms of selenite cytotoxicity in cancer cells are thought to be induced by metabolites of selenite. We observed that intracellular metabolism of selenite generates endogenous selenium nanoparticles (SeNPs) in c...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Bao P,Chen Z,Tai RZ,Shen HM,Martin FL,Zhu YG

    更新日期:2015-02-06 00:00:00

  • Comparison of software tools to improve the detection of carcinogen induced changes in the rat liver proteome by analyzing SELDI-TOF-MS spectra.

    abstract::A common animal model of chemical hepatocarcinogenesis was used to demonstrate the potential identification of carcinogenicity related protein signatures/biomarkers. Therefore, an animal study in which rats were treated with the known liver carcinogen N-nitrosomorpholine (NNM) or the corresponding vehicle was evaluate...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Beyer S,Walter Y,Hellmann J,Kramer PJ,Kopp-Schneider A,Kroeger M,Ittrich C

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  • Quantitative Proteomics Reveals Changes in Vero Cells in Response to Porcine Epidemic Diarrhea Virus.

    abstract::Outbreaks of porcine epidemic diarrhea virus (PEDV) have caused significant lethality rates in neonatal piglets, which pose a serious threat to the swine industry worldwide. Available commercial vaccines fail to protect against the emergence of high virulence of PEDV variants. Therefore, the endemic state of the PEDV ...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Ye Y,Zhu J,Ai Q,Wang C,Liao M,Fan H

    更新日期:2019-04-05 00:00:00

  • The human colostrum whey proteome is altered in gestational diabetes mellitus.

    abstract::Proteomics of human milk has been used to identify the comprehensive cargo of proteins involved in immune and cellular function. Very little is known about the effects of gestational diabetes mellitus (GDM) on lactation and breast milk components. The objective of the current study was to examine the effect of GDM on ...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Grapov D,Lemay DG,Weber D,Phinney BS,Azulay Chertok IR,Gho DS,German JB,Smilowitz JT

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  • Standardized protocols for quality control of MRM-based plasma proteomic workflows.

    abstract::Mass spectrometry (MS)-based proteomics is rapidly emerging as a viable technology for the identification and quantitation of biological samples, such as human plasma--the most complex yet commonly employed biofluid in clinical analyses. The transition from a qualitative to quantitative science is required if proteomi...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Percy AJ,Chambers AG,Smith DS,Borchers CH

    更新日期:2013-01-04 00:00:00

  • Proteomic analysis of human serum by two-dimensional differential gel electrophoresis after depletion of high-abundant proteins.

    abstract::Two-dimensional differential gel electrophoresis (2-D DIGE) was used to analyze human serum following the removal of albumin and five other high-abundant serum proteins. After protein removal, serum was analyzed by SDS-PAGE as a preliminary screen, and significant differences between four high-abundant protein removal...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Chromy BA,Gonzales AD,Perkins J,Choi MW,Corzett MH,Chang BC,Corzett CH,McCutchen-Maloney SL

    更新日期:2004-11-01 00:00:00

  • LC-MS/MS characterization of O-glycosylation sites and glycan structures of human cerebrospinal fluid glycoproteins.

    abstract::The GalNAc O-glycosylation on Ser/Thr residues of extracellular proteins has not been well characterized from a proteomics perspective. We previously reported a sialic acid capture-and-release protocol to enrich tryptic N- and O-glycopeptides from human cerebrospinal fluid glycoproteins using nano-LC-ESI-MS/MS with co...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Halim A,Rüetschi U,Larson G,Nilsson J

    更新日期:2013-02-01 00:00:00

  • Mapping Citrullinated Sites in Multiple Organs of Mice Using Hypercitrullinated Library.

    abstract::Protein citrullination (or deimination), an irreversible post-translational modification, has been implicated in several physiological and pathological processes, including gene expression regulation, apoptosis, rheumatoid arthritis, and Alzheimer's disease. Several research studies have been carried out on citrullina...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Fert-Bober J,Venkatraman V,Hunter CL,Liu R,Crowgey EL,Pandey R,Holewinski RJ,Stotland A,Berman BP,Van Eyk JE

    更新日期:2019-05-03 00:00:00

  • Discovery of Manassantin A Protein Targets Using Large-Scale Protein Folding and Stability Measurements.

    abstract::Manassantin A is a natural product that has been shown to have anticancer activity in cell-based assays, but has a largely unknown mode-of-action. Described here is the use of two different energetics-based approaches to identify protein targets of manassantin A. Using the stability of proteins from rates of oxidation...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Geer Wallace MA,Kwon DY,Weitzel DH,Lee CT,Stephenson TN,Chi JT,Mook RA Jr,Dewhirst MW,Hong J,Fitzgerald MC

    更新日期:2016-08-05 00:00:00

  • Quantitative Phosphoproteomics Revealed Glucose-Stimulated Responses of Islet Associated with Insulin Secretion.

    abstract::As central tissue of glucose homeostasis, islet has been an important focus of diabetes research. Phosphorylation plays pivotal roles in islet function, especially in islet glucose-stimulated insulin secretion. A systematic view on how phosphorylation networks were coordinately regulated in this process remains lackin...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Li J,Li Q,Tang J,Xia F,Wu J,Zeng R

    更新日期:2015-11-06 00:00:00

  • Proteomic and Metabolomic Analyses of Leaf from Clematis terniflora DC. Exposed to High-Level Ultraviolet-B Irradiation with Dark Treatment.

    abstract::Clematis terniflora DC. has potential pharmaceutical value; on the contrary, high-level UV-B irradiation with dark treatment led to the accumulation of secondary metabolites. Metabolomic and proteomic analyses of leaf of C. terniflora were performed to investigate the systematic response mechanisms to high-level UV-B ...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Yang B,Wang X,Gao C,Chen M,Guan Q,Tian J,Komatsu S

    更新日期:2016-08-05 00:00:00

  • Serum glycome profiling: a biomarker for diagnosis of ovarian cancer.

    abstract::During the development of cancer, changes in cellular glycosylation are observed, indicating that alterations of the glycome occur in extracellular fluids as well as in serum and could therefore serve as tumor biomarkers. In the case of epithelial ovarian cancer (EOC), common tumor markers such as CA125 are known to h...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Biskup K,Braicu EI,Sehouli J,Fotopoulou C,Tauber R,Berger M,Blanchard V

    更新日期:2013-09-06 00:00:00

  • Phosphoproteome study reveals Hsp27 as a novel signaling molecule involved in GDNF-induced neurite outgrowth.

    abstract::Glial-cell-line-derived neurotrophic factor (GDNF) is a most potent survival factor for dopaminergic neurons. In addition, GDNF was also found to promote neurite outgrowth in dopaminergic neurons. However, despite the potential clinical and physiological importance of GDNF, its mechanism of action is unclear. Therefor...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Hong Z,Zhang QY,Liu J,Wang ZQ,Zhang Y,Xiao Q,Lu J,Zhou HY,Chen SD

    更新日期:2009-06-01 00:00:00

  • Multicolumn separation platform for simultaneous depletion and prefractionation prior to 2-DE for facilitating in-depth serum proteomics profiling.

    abstract::In this report, we describe an integrated fluidic platform composed of tandem affinity columns for the depletion of high-abundance proteins from human serum and on-line fractionation/concentration of medium- and low-abundance proteins by tandem immobilized metal-ion affinity chromatography (IMAC) columns and reversed ...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Jmeian Y,El Rassi Z

    更新日期:2009-10-01 00:00:00