Phenotyping polyclonal kappa and lambda light chain molecular mass distributions in patient serum using mass spectrometry.

abstract：:NMR ligand affinity screening is a powerful technique that is routinely used in drug discovery or functional genomics to directly detect protein-ligand binding events. Binding events can be identified by monitoring differences in the 1D (1)H NMR spectrum of a compound with and without protein. Although a single NMR sp...

journal_title：Journal of proteome research

authors： Stark JL,Eghbalnia HR,Lee W,Westler WM,Markley JL

更新日期：2016-04-01 00:00:00

abstract：:Nanobacteria may cause peripheral neuropathy by adhesion to the perineurium. This hypothesis receives support from five independent observations: (1) identification of perineurial apatite in diabetic patients with peripheral neuropathy, (2) massive presence of nanobacteria in a diabetic patient, (3) beneficial effect ...

journal_title：Journal of proteome research

authors： Sommer AP

更新日期：2004-09-01 00:00:00

abstract：:Starting in 2007, we have grown soybean (Glycine max [L.] Merr. variety Soniachna) and flax (Linum usitatissimum, L. variety Kyivskyi) in the radio-contaminated Chernobyl area and analyzed the seed proteomes. In the second-generation flax seeds, we detected a 12% increase in oil content. To characterize the bases for ...

journal_title：Journal of proteome research

authors： Klubicová K,Danchenko M,Skultety L,Berezhna VV,Rashydov NM,Hajduch M

更新日期：2013-11-01 00:00:00

abstract：:The deuterium, a frequently used stable isotope in isotopic labeling for quantitative proteomics, could deteriorate the accuracy and precision of proteome quantification owing to the retention time shift of deuterated peptides from the hydrogenated counterpart. We introduce a novel three-plexed peptide "diethylation" ...

journal_title：Journal of proteome research

authors： Jung J,Jeong K,Choi Y,Kim SA,Kim H,Lee JW,Kim VN,Kim KP,Kim JS

更新日期：2019-03-01 00:00:00

abstract：:Diosgenin (DIO), the starting material for the synthesis of steroidal anti-inflammatory drugs in the pharmaceutical industry, has been previously demonstrated to display pharmaceutical effects against cerebral ischemic reperfusion (I/R). However, the alterations of brain proteome profiles underlying this treatment rem...

journal_title：Journal of proteome research

authors： Zhang X,Wang X,Khurm M,Zhan G,Zhang H,Ito Y,Guo Z

更新日期：2020-03-06 00:00:00

abstract：:In the framework of the C-HPP, our Franco-Swiss consortium has adopted chromosomes 2 and 14, coding for a total of 382 missing proteins (proteins for which evidence is lacking at protein level). Over the last 4 years, the French proteomics infrastructure has collected high-quality data sets from 40 human samples, incl...

journal_title：Journal of proteome research

authors： Carapito C,Lane L,Benama M,Opsomer A,Mouton-Barbosa E,Garrigues L,Gonzalez de Peredo A,Burel A,Bruley C,Gateau A,Bouyssié D,Jaquinod M,Cianferani S,Burlet-Schiltz O,Van Dorsselaer A,Garin J,Vandenbrouck Y

更新日期：2015-09-04 00:00:00

abstract：:Protein arrays will greatly accelerate research and development in medical and biological sciences. We have used cell-free protein biosynthesis and a parallel immobilization strategy for producing protein biochips. We demonstrate a model two-protein microarray using luciferase and green fluorescent protein, both expre...

journal_title：Journal of proteome research

authors： Oleinikov AV,Gray MD,Zhao J,Montgomery DD,Ghindilis AL,Dill K

更新日期：2003-05-01 00:00:00

abstract：:A major source of intestinal metabolites results from both host and microbial processing of dietary nutrients. (1)H NMR-based metabolic profiling of mouse feces was carried out over time in different microbiome mouse models, including conventional (n = 9), conventionalized (n = 10), and "humanized" gnotobiotic mice in...

journal_title：Journal of proteome research

authors： Martin FP,Sprenger N,Montoliu I,Rezzi S,Kochhar S,Nicholson JK

更新日期：2010-10-01 00:00:00

abstract：:We have explored the potential of commercial polystyrene-divinylbenzene monolithic capillary nanoLC-MS/MS for identifying Sinorhizobium fredii HH103 nodulation outer proteins. Monolithic nanoLC with off-line MALDI-TOF/TOF and on-line ESI-q-oTOF is fast and robust, generating complementary data and offering high-confid...

journal_title：Journal of proteome research

authors： Rodrigues JA,López-Baena FJ,Ollero FJ,Vinardell JM,Espuny Mdel R,Bellogín RA,Ruiz-Sainz JE,Thomas JR,Sumpton D,Ault J,Thomas-Oates J

更新日期：2007-03-01 00:00:00

abstract：:Cellular functions are largely carried out by noncovalent protein complexes that may exist within the cell as stable modules or as assemblies of dynamically changing composition, whose formation and decomposition are triggered in response to extracellular stimuli. The protein constituents of complexes often exhibit po...

journal_title：Journal of proteome research

authors： Przybylski C,Jünger MA,Aubertin J,Radvanyi F,Aebersold R,Pflieger D

更新日期：2010-10-01 00:00:00

abstract：:Fast (120 s) and high-throughput (more than six samples at once) in-gel trypsin digestion of proteins using sonoreactor technology has been achieved. Successful protein identification was done by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, MALDI-TOF-MS. Specific identification of the ...

journal_title：Journal of proteome research

authors： Rial-Otero R,Carreira RJ,Cordeiro FM,Moro AJ,Fernandes L,Moura I,Capelo JL

更新日期：2007-02-01 00:00:00

abstract：:Schwann cells (SCs) are the principal glial cells of the peripheral nervous system (PNS). As a result of tissue heterogeneity and difficulties in the isolation and culture of primary SCs, a considerable understanding of SC biology is obtained from SC lines. However, the differences between the primary SCs and SC lines...

journal_title：Journal of proteome research

authors： Ji Y,Shen M,Wang X,Zhang S,Yu S,Chen G,Gu X,Ding F

更新日期：2012-06-01 00:00:00

abstract：:"Dry eye" is a multifactorial inflammatory disease affecting the ocular surface. Tear hyperosmolarity in dry eye contributes to inflammation and cell damage. Recent research efforts on dry eye have been directed toward biomarker discovery for diagnosis, response to treatment, and disease mechanisms. This study employe...

journal_title：Journal of proteome research

authors： Chen L,Li J,Guo T,Ghosh S,Koh SK,Tian D,Zhang L,Jia D,Beuerman RW,Aebersold R,Chan EC,Zhou L

更新日期：2015-09-04 00:00:00

abstract：:Pancreatic cancer is a formidable disease and early detection biomarkers are needed to make inroads into improving the outcomes in these patients. In this work, lectin antibody microarrays were utilized to detect unique glycosylation patterns of proteins from serum. Antibodies to four potential glycoprotein markers th...

journal_title：Journal of proteome research

authors： Li C,Simeone DM,Brenner DE,Anderson MA,Shedden KA,Ruffin MT,Lubman DM

更新日期：2009-02-01 00:00:00

abstract：:Profiling of tissues and cell types through systematic characterization of expressed genes or proteins shows promise as a basic research tool, and has potential applications in disease diagnosis and classification. We used multidimensional protein identification protein identification technology (MudPIT) to analyze pr...

journal_title：Journal of proteome research

authors： Cagney G,Park S,Chung C,Tong B,O'Dushlaine C,Shields DC,Emili A

更新日期：2005-09-01 00:00:00

abstract：:An efficient means for the identification of prognostic and predictive biomarkers is essential in today's cancer management. A new approach toward biomarker discovery has therefore been proposed, where pathways instead of individual proteins would be monitored and targeted. Recently, the 'secretome', a biological flui...

journal_title：Journal of proteome research

authors： Lawlor K,Nazarian A,Lacomis L,Tempst P,Villanueva J

更新日期：2009-03-01 00:00:00

abstract：:In type-1 diabetes mellitus (T1DM) with diabetic nephropathy (DN), accumulation of abnormal proteins in the kidney and other tissues may derive from constitutive alterations of intracellular protein recognition, assembly, and turnover. We characterized the proteins involved in these functions in cultured skin fibrobla...

journal_title：Journal of proteome research

authors： Tessari P,Puricelli L,Iori E,Arrigoni G,Vedovato M,James P,Coracina A,Millioni R

更新日期：2007-03-01 00:00:00

abstract：:Beta-thalassemia results from mutations of the β-hemoglobin (Hbb) gene and reduced functional Hbb synthesis. Excess α-Hb causes globin chain aggregation, oxidation, cytoskeletal damage, and increased red blood cell clearance. These events result in anemia, altered iron homeostasis, and expansion of extramedullary eryt...

journal_title：Journal of proteome research

authors： Vallelian F,Gelderman-Fuhrmann MP,Schaer CA,Puglia M,Opitz L,Baek JH,Vostal J,Buehler PW,Schaer DJ

更新日期：2015-02-06 00:00:00

abstract：:The ability of concentrated formic acid to formylate reactive amino acid residues is known from previous reports. In contrast, solvents containing a low concentration of formic acid are generally recognized as a safe environment for proteomic applications. The primary objective of this study was to explain the excessi...

journal_title：Journal of proteome research

authors： Lenčo J,Khalikova MA,Švec F

更新日期：2020-03-06 00:00:00

abstract：:We have obtained the first direct electrochemistry of full-length inducible nitric oxide synthase (iNOS) by entrapping the enzyme in polyethylenimine (PEI) film. The interaction between iNOS and calmodulin (CaM) was then studied, which revealed an enhanced electron-transfer reactivity of the enzyme facilitated by CaM....

journal_title：Journal of proteome research

authors： Xiao H,Zhou H,Chen G,Liu S,Li G

更新日期：2007-04-01 00:00:00

abstract：:There is a crucial need for development of prognostic and predictive biomarkers in human bladder carcinogenesis in order to personalize preventive and therapeutic strategies and improve outcomes. Epigenetic alterations, such as histone modifications, are implicated in the genetic dysregulation that is fundamental to c...

journal_title：Journal of proteome research

authors： Telu KH,Abbaoui B,Thomas-Ahner JM,Zynger DL,Clinton SK,Freitas MA,Mortazavi A

更新日期：2013-07-05 00:00:00

abstract：:Rhizobia, the nitrogen-fixing bacterial symbionts of legumes, represent an agricultural application of primary relevance and a model of plant-microbe molecular dialogues. We recently described rhizobium proteome alterations induced by plant flavonoids using iTRAQ. Herein, we further extend that experimentation, provin...

journal_title：Journal of proteome research

authors： Arrigoni G,Tolin S,Moscatiello R,Masi A,Navazio L,Squartini A

更新日期：2013-11-01 00:00:00

abstract：:The use of matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) to acquire spectral profiles has become a common approach to detect proteomic biomarkers of disease. MALDI-MS signals may represent both intact proteins as well as proteolysis products. Liquid chromatography-tandem mass spectrometry (L...

journal_title：Journal of proteome research

authors： Zimmerman LJ,Wernke GR,Caprioli RM,Liebler DC

更新日期：2005-09-01 00:00:00

abstract：:As work on systematic searches for developmentally-regulated proteins is limited, whole brains of CL57B/6J mice were taken from day 16 of gestation, at postnatal days 7 and 54-58. Proteins were extracted, and analyzed by a gel-based proteomic method (ion trap HCT). Antioxidant, cytoskeleton, chaperone, several metabol...

journal_title：Journal of proteome research

authors： Myung JK,Shim KS,Li L,Höger H,Lubec G

更新日期：2009-03-01 00:00:00

abstract：:Natural killer (NK) cells are important components of innate immune defense. NK cells kill virus-infected cells and secrete cytokines that are involved in activation of other immune cells. Macrophage-derived cytokines interferon-alpha (IFN-alpha) and interleukin-15 (IL-15) are in turn important activators of NK cells,...

journal_title：Journal of proteome research

authors： Rakkola R,Matikainen S,Nyman TA

更新日期：2005-01-01 00:00:00

abstract：:Acute stress may trigger systemic biochemical and physiological changes in living organisms, leading to a rapid loss of homeostasis, which can be gradually reinstated by self-regulatory mechanisms and/or drug intervention strategy. However, such a sophisticated metabolic regulatory process has so far been poorly under...

journal_title：Journal of proteome research

authors： Wang X,Su M,Qiu Y,Ni Y,Zhao T,Zhou M,Zhao A,Yang S,Zhao L,Jia W

更新日期：2007-09-01 00:00:00

abstract：:Formalin-fixed, paraffin-embedded (FFPE) tissues have been used to discover disease-associated protein changes using mass spectrometry. Protein post-translational modifications such as glycosylation are known to associate with disease development. In this study, we investigated whether FFPE tissues preserve such modif...

journal_title：Journal of proteome research

authors： Tian Y,Gurley K,Meany DL,Kemp CJ,Zhang H

更新日期：2009-04-01 00:00:00

abstract：:Searching tandem mass spectra against a peptide database requires accurate knowledge of various experimental parameters, including machine settings and details of the sample preparation protocol. In some cases, such as in reanalysis of public data sets, this experimental metadata may be missing or inaccurate. We descr...

journal_title：Journal of proteome research

authors： May DH,Tamura K,Noble WS

更新日期：2019-04-05 00:00:00

abstract：:Mass spectrometry (MS) has become an accessible tool for whole proteome quantitation with the ability to characterize protein expression across thousands of proteins within a single experiment. A subset of MS quantification methods (e.g., SILAC and label-free) monitor the relative intensity of intact peptides, where t...

journal_title：Journal of proteome research

authors： O'Brien JJ,O'Connell JD,Paulo JA,Thakurta S,Rose CM,Weekes MP,Huttlin EL,Gygi SP

更新日期：2018-01-05 00:00:00

abstract：:The identification of commercial shrimp species is a relevant issue to ensure correct labeling, maintain consumer confidence and enhance the knowledge of the captured species, benefiting both, fisheries and manufacturers. A proteomic approach, based on 2DE, tryptic in-gel digestion, MALDI-TOF MS, and ESI-MS/MS analyse...

journal_title：Journal of proteome research

authors： Ortea I,Cañas B,Gallardo JM

更新日期：2009-11-01 00:00:00