Abstract：
:Liquid chromatography-tandem mass spectrometry (LC-MS/MS) and multiple reaction monitoring mass spectrometry (MRM-MS) proteomics analyses were performed on eccrine sweat of healthy controls, and the results were compared with those from individuals diagnosed with schizophrenia (SZ). This is the first large scale study of the sweat proteome. First, we performed LC-MS/MS on pooled SZ samples and pooled control samples for global proteomics analysis. Results revealed a high abundance of diverse proteins and peptides in eccrine sweat. Most of the proteins identified from sweat samples were found to be different than the most abundant proteins from serum, which indicates that eccrine sweat is not simply a plasma transudate and may thereby be a source of unique disease-associated biomolecules. A second independent set of patient and control sweat samples were analyzed by LC-MS/MS and spectral counting to determine qualitative protein differential abundances between the control and disease groups. Differential abundances of selected proteins, initially determined by spectral counting, were verified by MRM-MS analyses. Seventeen proteins showed a differential abundance of approximately 2-fold or greater between the SZ pooled sample and the control pooled sample. This study demonstrates the utility of LC-MS/MS and MRM-MS as a viable strategy for the discovery and verification of potential sweat protein disease biomarkers.
journal_name
J Proteome Resjournal_title
Journal of proteome researchauthors
Raiszadeh MM,Ross MM,Russo PS,Schaepper MA,Zhou W,Deng J,Ng D,Dickson A,Dickson C,Strom M,Osorio C,Soeprono T,Wulfkuhle JD,Petricoin EF,Liotta LA,Kirsch WMdoi
10.1021/pr2007957subject
Has Abstractpub_date
2012-04-06 00:00:00pages
2127-39issue
4eissn
1535-3893issn
1535-3907journal_volume
11pub_type
杂志文章abstract：:Mass spectrometry is the main analytical technique currently used to address the challenges of glycomics as it offers unrivalled levels of sensitivity and the ability to handle complex mixtures of different glycan variations. Determination of glycan structures from analysis of MS data is a major bottleneck in high-thr...
journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr7008252
更新日期：2008-04-01 00:00:00
abstract：:An algorithm for protein identification based on mass spectrometric proteolytic peptide mapping and genome database searching is presented. The algorithm ranks database proteins based on direct calculation of the probability of random matching and assigns the statistical significance to each result. We investigate the...
journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr034048y
更新日期：2004-01-01 00:00:00
abstract：:Proteomics has been applied to study intracellular bacteria and phagocytic vacuoles in different host cell lines, especially macrophages (Mφs). For mycobacterial phagosomes, few studies have identified over several hundred proteins for systems assessment of the phagosome maturation and antigen presentation pathways. M...
journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr101245u
更新日期：2011-05-06 00:00:00
abstract：:POPX2 is a serine/threonine phosphatase belonging to the protein phosphatase 2C (PP2C) family that has been found to be elevated in invasive breast cancer cells. Silencing of POPX2 results in lower cell motility and invasiveness. The molecular mechanism of POPX2-regulated cell motility is not well understood. To ident...
journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr301113c
更新日期：2013-06-07 00:00:00
abstract：:Ralstonia eutropha H16 is a denitrifying microorganism able to use nitrate and nitrite as terminal electron acceptors under oxygen deprivation. To identify proteins showing an altered expression pattern in response to oxygen supply, R. eutropha cells grown aerobically and anaerobically were compared in a comprehensive...
journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr500491r
更新日期：2014-10-03 00:00:00
abstract：:Dysregulated protein phosphorylation is a primary culprit in multiple physiopathological states. Hence, although analysis of signaling cascades on a proteome-wide scale would provide significant insight into both normal and aberrant cellular function, such studies are simultaneously limited by sheer biological complex...
journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr0602803
更新日期：2006-10-01 00:00:00
abstract：:Recoding refers to the reprogramming of mRNA translation by nonstandard read-out rules. In this study, we used stable isotope labeling with amino acids in cell culture (SILAC) technology to investigate the proteome of host-adapted Salmonella serovars, which are characteristic of accumulation of pseudogenes. Interestin...
journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr200904c
更新日期：2012-03-02 00:00:00
abstract：:New mass-tagging reagents for quantitative proteomics measurements have been designed using solid phase peptide synthesis technology. The solid phase mass tags have been used to accurately measure the relative amounts of cysteine-containing peptides in model peptide mixtures as well as in mixtures of tryptic digests i...
journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr034081k
更新日期：2004-01-01 00:00:00
abstract：:Accurate predictions of peptide retention times (RT) in liquid chromatography have many applications in mass spectrometry-based proteomics. Most notably such predictions are used to weed out incorrect peptide-spectrum matches, and to design targeted proteomics experiments. In this study, we describe a RT predictor, EL...
journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr1005058
更新日期：2010-10-01 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr050346e
更新日期：2006-02-01 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/acs.jproteome.5b00249
更新日期：2015-07-02 00:00:00
abstract：:Compartment proteomics enable broad characterization of target tissues. We employed a simple fractionation method and filter-aided sample preparation (FASP) to characterize the cytosolic and membrane fractions of white perch ovary tissues by semiquantitative tandem mass spectrometry using label-free quantitation based...
journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr401067g
更新日期：2014-03-07 00:00:00
abstract：:Metal based chemotherapeutic drugs are widely used as an effective method to defeat various cancers. In this study, the mechanism of action of a novel therapeutic agent, [Pd(sac)(terpy)](sac)·4H2O (sac = saccharinate, and terpy = 2,2':6',2″-terpyridine) was studied. To better understand the proteomic changes in respon...
journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr5006718
更新日期：2014-11-07 00:00:00
abstract：:A popular method for peptide quantification relies on isobaric labeling such as tandem mass tags (TMT), which enables multiplexed proteome analyses. Quantification is achieved by reporter ions generated by fragmentation in a tandem mass spectrometer. However, with higher degrees of multiplexing, the smaller mass diffe...
journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/acs.jproteome.8b00381
更新日期：2018-11-02 00:00:00
abstract：:The identification of commercial shrimp species is a relevant issue to ensure correct labeling, maintain consumer confidence and enhance the knowledge of the captured species, benefiting both, fisheries and manufacturers. A proteomic approach, based on 2DE, tryptic in-gel digestion, MALDI-TOF MS, and ESI-MS/MS analyse...
journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr900663d
更新日期：2009-11-01 00:00:00
abstract：:Elevated atmospheric CO2 and nitrogen are major environmental factors affecting shoot growth. The objectives of this study are to determine the interactive effects of elevated CO2 and nitrogen on leaf growth in tall fescue ( Festuca arundinacea) and to identify major proteins and associated metabolic pathways underlyi...
journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/acs.jproteome.8b00951
更新日期：2019-06-07 00:00:00
abstract：:Proteins are continuously exposed to diverse chemical stresses, and the resulting chemical modifications can provide significant information on biological events. Keratins are the main constituent of human skin and are the major target proteins of various chemical modifications. We have previously developed a mass spe...
journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/acs.jproteome.0c00406
更新日期：2020-09-04 00:00:00
abstract：:Facing the explosion of newly generated protein sequences in the post genomic era, we are challenged to develop an automated method for fast and reliably annotating their subcellular locations. Knowledge of subcellular locations of proteins can provide useful hints for revealing their functions and understanding how t...
journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr060167c
更新日期：2006-08-01 00:00:00
abstract：:There is a legion of hypothetical proteins (HP) in prokaryotic and eukaryotic proteomes and the aim of this study was to describe HP in the perireticular nucleus (PN), a key structure in human brain development. Tissue from four PNs was homogenized and extracted proteins were run on two-dimensional gel electrophoresis...
journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr050133p
更新日期：2005-11-01 00:00:00
abstract：:The plasma of patients with stable carotid atherosclerosis (n = 9), and healthy subjects (n = 10) have been fingerprinted with both GC-MS and (1)H NMR. Principal component analysis (PCA), partial least-squares-discriminant analysis (PLS-DA) and orthogonal partial least-squares-discriminant analysis (OPLS-DA) have been...
journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr900668v
更新日期：2009-12-01 00:00:00
abstract：:Direct tissue profiling and imaging mass spectrometry (MS) provides a detailed assessment of the complex protein pattern within a tissue sample. MALDI MS analysis of thin tissue sections results in over of 500 individual protein signals in the mass range of 2 to 70 kDa that directly correlate with protein composition ...
journal_title：Journal of proteome research
pub_type： 杂志文章,评审
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更新日期：2004-03-01 00:00:00
abstract：:Ceramides are central molecules in sphingolipid metabolism. They are involved in the regulation of cancer-cell growth, differentiation, senescence and apoptosis. To better understand how these secondary messengers induce their biological effects, adenocarcinoma cells (HCT116) were treated with exogenous long-chain cer...
journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr9005316
更新日期：2009-10-01 00:00:00
abstract：:Advances in proteomics are continually driven by the introduction of new mass spectrometric instrumentation with improved performances. The recently introduced quadrupole Orbitrap (Q Exactive) tandem mass spectrometer allows fast acquisition of high-resolution higher-energy collisional dissociation (HCD) tandem mass s...
journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr3000249
更新日期：2012-06-01 00:00:00
abstract：:Transient cerebral ischemia dramatically activates small ubiquitin-like modifier (SUMO2/3) conjugation. In cells exposed to 6 h of transient oxygen/glucose deprivation (OGD), a model of ischemia, SUMOylation increases profoundly between 0 and 30 min following re-oxygenation. To elucidate the effect of transient OGD on...
journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr200834f
更新日期：2012-02-03 00:00:00
abstract：:The GalNAc O-glycosylation on Ser/Thr residues of extracellular proteins has not been well characterized from a proteomics perspective. We previously reported a sialic acid capture-and-release protocol to enrich tryptic N- and O-glycopeptides from human cerebrospinal fluid glycoproteins using nano-LC-ESI-MS/MS with co...
journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr300963h
更新日期：2013-02-01 00:00:00
abstract：:Detergents are indispensable solubilizing agents in the purification and analysis of membrane proteins. For mass spectrometric identification of proteins, it is essential that detergents are removed prior to analysis, necessitating an in-gel digestion step. Here, we report a procedure that allows use of detergents and...
journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr800412j
更新日期：2008-11-01 00:00:00
abstract：:There are new challenges for hazard and risk assessment in the chemical industry with regard to REACH legislation in Europe and related activities in the U.S. and Japan, which require the development of novel in vitro models for the molecular characterization of drug- or chemical-related effects replacing conventional...
journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr100514e
更新日期：2010-11-05 00:00:00
abstract：:Birth weight is an important indicator of maternal and fetal health and a predictor of health in later life. However, the determinants of variance in birth weight are still poorly understood. We aimed to identify the biological pathways, which may be perturbed by environmental exposures, that are important in determin...
journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/acs.jproteome.7b00846
更新日期：2018-03-02 00:00:00
abstract：:Src family kinases (SFKs) are the earliest known family of tyrosine kinases and are widely thought to play essential roles in cellular signal transduction. Although numerous functional analyses have been performed, no study has analyzed the specificity of all SFKs on an equal platform. To gain a better understanding o...
journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr100773t
更新日期：2010-11-05 00:00:00
abstract：:Histones regulate a variety of chromatin templated events by their post-translational modifications (PTMs). Although there are extensive reports on the PTMs of canonical histones, the information on the histone variants remains very scanty. Here, we report the identification of different PTMs, such as acetylation, met...
journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr500597a
更新日期：2014-12-05 00:00:00
