Abstract：
:As central tissue of glucose homeostasis, islet has been an important focus of diabetes research. Phosphorylation plays pivotal roles in islet function, especially in islet glucose-stimulated insulin secretion. A systematic view on how phosphorylation networks were coordinately regulated in this process remains lacking, partially due to the limited amount of islets from an individual for a phosphoproteomic analysis. Here we optimized the in-tip and best-ratio phosphopeptide enrichment strategy and a SILAC-based workflow for processing rat islet samples. With limited islet lysates from each individual rat (20-47 μg), we identified 8539 phosphosites on 2487 proteins. Subsequent quantitative analyses uncovered that short-term (30 min) high glucose stimulation induced coordinate responses of islet phosphoproteome on multiple biological levels, including insulin secretion related pathways, cytoskeleton dynamics, protein processing in ER and Golgi, transcription and translation, and so on. Furthermore, three glucose-responsive phosphosites (Prkar1a pT75pS77 and Tagln2 pS163) from the data set were proved to be correlated with insulin secretion. Overall, we initially gave an in-depth map of islet phosphoproteome regulated by glucose on individual rat level. This was a significant addition to our knowledge about how phosphorylation networks responded in insulin secretion. Also, the list of changed phosphosites was a valuable resource for molecular researchers in diabetes field.
journal_name
J Proteome Resjournal_title
Journal of proteome researchauthors
Li J,Li Q,Tang J,Xia F,Wu J,Zeng Rdoi
10.1021/acs.jproteome.5b00507subject
Has Abstractpub_date
2015-11-06 00:00:00pages
4635-46issue
11eissn
1535-3893issn
1535-3907journal_volume
14pub_type
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journal_title：Journal of proteome research
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更新日期：2013-08-02 00:00:00
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更新日期：2008-01-01 00:00:00
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更新日期：2013-03-01 00:00:00
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pub_type： 杂志文章
doi：10.1021/pr050002o
更新日期：2005-09-01 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章,评审
doi：10.1021/acs.jproteome.9b00228
更新日期：2019-06-07 00:00:00
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pub_type： 杂志文章
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更新日期：2007-03-01 00:00:00
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pub_type： 杂志文章
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更新日期：2012-06-01 00:00:00
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doi：10.1021/pr025568g
更新日期：2003-03-01 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr049857b
更新日期：2005-01-01 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr050122w
更新日期：2005-07-01 00:00:00
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更新日期：2011-12-02 00:00:00
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