Proteins associated with immunopurified granules from a model pancreatic islet beta-cell system: proteomic snapshot of an endocrine secretory granule.

abstract：:An efficient means for the identification of prognostic and predictive biomarkers is essential in today's cancer management. A new approach toward biomarker discovery has therefore been proposed, where pathways instead of individual proteins would be monitored and targeted. Recently, the 'secretome', a biological flui...

journal_title：Journal of proteome research

authors： Lawlor K,Nazarian A,Lacomis L,Tempst P,Villanueva J

更新日期：2009-03-01 00:00:00

abstract：:Oxidation generates multiple diverse post-translational modifications resulting in changes in protein structure and function associated with a wide range of diseases. Of these modifications, carbonylations have often been used as hallmarks of oxidative damage. However, accumulating evidence supports the hypothesis tha...

journal_title：Journal of proteome research

authors： Rykær M,Svensson B,Davies MJ,Hägglund P

更新日期：2017-11-03 00:00:00

abstract：:The balance between comprehensively analyzing the proteome and using valuable mass spectrometry time is a genuine challenge in the field of proteomics. Multidimensional fractionation strategies have significantly increased proteome coverage, but often at the cost of increased mass analysis time, despite advances in ma...

journal_title：Journal of proteome research

authors： Krieger JR,Wybenga-Groot LE,Tong J,Bache N,Tsao MS,Moran MF

更新日期：2019-05-03 00:00:00

abstract：:Minimal hepatic encephalopathy (MHE) reduces quality of life of cirrhotic patients, predicts overt hepatic encephalopathy, and is associated with poor prognosis. We hypothesized that MHE arises once metabolic alterations derived from the liver reach a particular threshold. Our aim was to assess whether metabolic profi...

journal_title：Journal of proteome research

authors： Jiménez B,Montoliu C,MacIntyre DA,Serra MA,Wassel A,Jover M,Romero-Gomez M,Rodrigo JM,Pineda-Lucena A,Felipo V

更新日期：2010-10-01 00:00:00

abstract：:We have investigated whether postexercise ingestion of carbohydrates in combination with proteins generates a different systemic metabolic response, as compared to the sole ingestion of carbohydrate or water, in the early recovery phase following exercise. In addition, metabolic patterns related to fitness level were ...

journal_title：Journal of proteome research

authors： Chorell E,Moritz T,Branth S,Antti H,Svensson MB

更新日期：2009-06-01 00:00:00

abstract：:We investigated the effect of homogenization strategy and protein precipitation on downstream protein quantitation using multiple reaction monitoring mass spectrometry (MRM-MS). Our objective was to develop a workflow capable of processing disparate tissue types with high throughput, minimal variability, and maximum p...

journal_title：Journal of proteome research

authors： Michaud SA,Pětrošová H,Jackson AM,McGuire JC,Sinclair NJ,Ganguly M,Flenniken AM,Nutter LMJ,McKerlie C,Schibli D,Smith D,Borchers CH

更新日期：2021-01-01 00:00:00

abstract：:One of the ultimate goals of systems biology research is to obtain a comprehensive understanding of the control mechanisms of complex cellular metabolisms. Metabolic Flux Analysis (MFA) is a important method for the quantitative estimation of intracellular metabolic flows through metabolic pathways and the elucidation...

journal_title：Journal of proteome research

authors： Toya Y,Kono N,Arakawa K,Tomita M

更新日期：2011-08-05 00:00:00

abstract：:Profiling of tissues and cell types through systematic characterization of expressed genes or proteins shows promise as a basic research tool, and has potential applications in disease diagnosis and classification. We used multidimensional protein identification protein identification technology (MudPIT) to analyze pr...

journal_title：Journal of proteome research

authors： Cagney G,Park S,Chung C,Tong B,O'Dushlaine C,Shields DC,Emili A

更新日期：2005-09-01 00:00:00

abstract：:Mass spectrometry is being used to identify protein biomarkers that can facilitate development of drug treatment. Mass spectrometry-based labeling proteomic experiments result in complex proteomic data that is hierarchical in nature often with small sample size studies. The generalized linear model (GLM) is the most p...

journal_title：Journal of proteome research

authors： D'Angelo G,Chaerkady R,Yu W,Hizal DB,Hess S,Zhao W,Lekstrom K,Guo X,White WI,Roskos L,Bowen MA,Yang H

更新日期：2017-09-01 00:00:00

abstract：:Moving from macroscale preparative systems in proteomics to micro- and nanotechnologies offers researchers the ability to deeply profile smaller numbers of cells that are more likely to be encountered in clinical settings. Herein a recently developed microscale proteomic method, microdroplet processing in one pot for ...

journal_title：Journal of proteome research

authors： Weke K,Singh A,Uwugiaren N,Alfaro JA,Wang T,Hupp TR,O'Neill JR,Vojtesek B,Goodlett DR,Williams SM,Zhou M,Kelly RT,Zhu Y,Dapic I

更新日期：2021-01-25 00:00:00

abstract：:The Model Organism Protein Expression Database (MOPED, http://moped.proteinspire.org) is an expanding proteomics resource to enable biological and biomedical discoveries. MOPED aggregates simple, standardized and consistently processed summaries of protein expression and metadata from proteomics (mass spectrometry) ex...

journal_title：Journal of proteome research

authors： Higdon R,Stewart E,Stanberry L,Haynes W,Choiniere J,Montague E,Anderson N,Yandl G,Janko I,Broomall W,Fishilevich S,Lancet D,Kolker N,Kolker E

更新日期：2014-01-03 00:00:00

abstract：:MSI is a molecular imaging technique that allows for the generation of topographic 2D maps for various endogenous and some exogenous molecules without prior specification of the molecule. In this paper, we start with the premise that a region of interest (ROI) is given to us based on preselected morphological criteria...

journal_title：Journal of proteome research

authors： Bruand J,Sistla S,Mériaux C,Dorrestein PC,Gaasterland T,Ghassemian M,Wisztorski M,Fournier I,Salzet M,Macagno E,Bafna V

更新日期：2011-04-01 00:00:00

abstract：:Vascular leakage is a hallmark of severe dengue infection. Although extensive studies have been conducted during the past several decades, the molecular mechanisms underlying vascular leakage in dengue shock syndrome (DSS) remain unclear. We thus performed a proteomics study to characterize responses in human endothel...

journal_title：Journal of proteome research

authors： Kanlaya R,Pattanakitsakul SN,Sinchaikul S,Chen ST,Thongboonkerd V

更新日期：2009-05-01 00:00:00

abstract：:Mitosis is a highly regulated process in which errors can lead to genomic instability, a hallmark of cancer. During this phase of the cell cycle, transcription is silent and RNA translation is inhibited. Thus, mitosis is largely driven by post-translational modification of proteins, including phosphorylation, methylat...

journal_title：Journal of proteome research

authors： Chuang C,Lin SH,Huang F,Pan J,Josic D,Yu-Lee LY

更新日期：2010-09-03 00:00:00

abstract：:Selenite has been a touted cancer chemopreventative agent but generates conflicting outcomes. Multiple mechanisms of selenite cytotoxicity in cancer cells are thought to be induced by metabolites of selenite. We observed that intracellular metabolism of selenite generates endogenous selenium nanoparticles (SeNPs) in c...

journal_title：Journal of proteome research

authors： Bao P,Chen Z,Tai RZ,Shen HM,Martin FL,Zhu YG

更新日期：2015-02-06 00:00:00

abstract：:In-source fragmentation occurs as a byproduct of electrospray ionization. We find that ions produced as a result of in-source fragmentation often match fragment ions produced during MS/MS fragmentation, and we take advantage of this phenomenon in a novel algorithm to analyze LC-MS metabolomics data sets. Our approach ...

journal_title：Journal of proteome research

authors： Seitzer PM,Searle BC

更新日期：2019-02-01 00:00:00

abstract：:Tumor invasion defines the transition between tissue-restricted carcinomas, related to good outcome as optimal surgery becomes possible, and metastatic tumors associated with poor prognosis and a dramatic decrease in survival. In endometrial cancer, myometrial infiltration represents a determinant parameter highly val...

journal_title：Journal of proteome research

authors： Monge M,Doll A,Colas E,Gil-Moreno A,Castellvi J,Garcia A,Colome N,Perez-Benavente A,Pedrola N,Lopez-Lopez R,Dolcet X,Ramon y Cajal S,Xercavins J,Matias-Guiu X,Canals F,Reventos J,Abal M

更新日期：2009-10-01 00:00:00

abstract：:Differentiating and quantifying protein differences in complex samples produces significant challenges in sensitivity and specificity. Label-free quantification can draw from two different information sources: precursor intensities and spectral counts. Intensities are accurate for calculating protein relative abundanc...

journal_title：Journal of proteome research

authors： Chen YY,Chambers MC,Li M,Ham AJ,Turner JL,Zhang B,Tabb DL

更新日期：2013-09-06 00:00:00

abstract：:Linear, short-chain polyfluorinated and perfluorinated alkyl compounds, often referred to as PFCs, have been in worldwide use as surfactants and polymer precursors for decades, and environmental dispersal of these highly persistent compounds represents a public health threat. Whereas ubiquitous low-level exposure to t...

journal_title：Journal of proteome research

authors： Hansmeier N,Chao TC,Herbstman JB,Goldman LR,Witter FR,Halden RU

更新日期：2015-01-02 00:00:00

abstract：:Today's highly accurate spectra provided by modern tandem mass spectrometers offer considerable advantages for the analysis of proteomic samples of increased complexity. Among other factors, the quantity of reliably identified peptides is considerably influenced by the peptide identification algorithm. While most wide...

journal_title：Journal of proteome research

authors： Dorfer V,Pichler P,Stranzl T,Stadlmann J,Taus T,Winkler S,Mechtler K

更新日期：2014-08-01 00:00:00

abstract：:Extracellular vesicles (EVs) are cell-derived microparticles present in most body fluids, mainly including microvesicles and exosomes. EV-harbored proteins have emerged as novel biomarkers for the diagnosis and prediction of different cancers. We successfully isolated microvesicles and exosomes from human saliva, whic...

journal_title：Journal of proteome research

authors： Sun Y,Huo C,Qiao Z,Shang Z,Uzzaman A,Liu S,Jiang X,Fan LY,Ji L,Guan X,Cao CX,Xiao H

更新日期：2018-03-02 00:00:00

abstract：:Immune recognition of nonself is coordinated through complex mechanisms involving both innate and adaptive responses. Circulating antibodies communicate with effector cells of the innate immune system through surface receptors known as Fcγ receptors (FcγRs). The FcγRs are single-pass transmembrane glycoproteins respon...

journal_title：Journal of proteome research

authors： Cosgrave EF,Struwe WB,Hayes JM,Harvey DJ,Wormald MR,Rudd PM

更新日期：2013-08-02 00:00:00

abstract：:Multiple reaction monitoring (MRM) is emerging as a promising technique for the detection and quantification of protein biomarkers in complex biological samples. Compared to Western blotting or enzyme assays, its high sensitivity, specificity, accuracy, assay speed, and sample throughput represent a clear advantage fo...

journal_title：Journal of proteome research

authors： Martínez-Márquez A,Morante-Carriel J,Sellés-Marchart S,Martínez-Esteso MJ,Pineda-Lucas JL,Luque I,Bru-Martínez R

更新日期：2013-12-06 00:00:00

abstract：:Metabolically engineered Escherichia coli has previously been used to degrade cis-1,2-dichloroethylene (cis-DCE). The strains express the six genes of an evolved toluene ortho-monooxygenase from Burkholderia cepacia G4 (TOM-Green, which formed a reactive epoxide) with either (1) gamma-glutamylcysteine synthetase (GSHI...

journal_title：Journal of proteome research

authors： Lee J,Cao L,Ow SY,Barrios-Llerena ME,Chen W,Wood TK,Wright PC

更新日期：2006-06-01 00:00:00

abstract：:Metabolomic approaches have the potential to make an exceptional contribution to understanding how chemicals and other environmental stressors can affect both human and environmental health. However, the application of metabolomics to environmental exposures, although getting underway, has not yet been extensively exp...

journal_title：Journal of proteome research

authors： Miller MG

更新日期：2007-02-01 00:00:00

abstract：:Ovarian cancer remains a deadly threat to women as the disease is often diagnosed in the late stages when the chance of survival is low. There are no good biomarkers available for early detection and only a few markers have shown clinical utility for prognosis, response to therapy and disease recurrence. We mined cond...

journal_title：Journal of proteome research

authors： Gunawardana CG,Kuk C,Smith CR,Batruch I,Soosaipillai A,Diamandis EP

更新日期：2009-10-01 00:00:00

abstract：:A role for Src Family Kinases (SFKs) in the dynamics of endocytic and secretory pathways has previously been reported. Identification of low-abundance compartmentalized complexes still remains challenging, highlighting the need for novel tools. Here we describe analysis of SFK-signaling complexes of hepatic Golgi/endo...

journal_title：Journal of proteome research

authors： Bilodeau N,Fiset A,Boulanger MC,Bhardwaj S,Winstall E,Lavoie JN,Faure RL

更新日期：2010-02-05 00:00:00

abstract：:Dysregulated protein phosphorylation is a primary culprit in multiple physiopathological states. Hence, although analysis of signaling cascades on a proteome-wide scale would provide significant insight into both normal and aberrant cellular function, such studies are simultaneously limited by sheer biological complex...

journal_title：Journal of proteome research

authors： Ndassa YM,Orsi C,Marto JA,Chen S,Ross MM

更新日期：2006-10-01 00:00:00

abstract：:In this report, we use a proteomic strategy to identify glycoproteins on the surface of exosomes derived from myeloid-derived suppressor cells (MDSCs), and then test if selected glycoproteins contribute to exosome-mediated chemotaxis and migration of MDSCs. We report successful modification of a surface chemistry meth...

journal_title：Journal of proteome research

authors： Chauhan S,Danielson S,Clements V,Edwards N,Ostrand-Rosenberg S,Fenselau C

更新日期：2017-01-06 00:00:00

abstract：:An unusual sulfotyrosine-, phosphoserine-containing motif was mapped on a differentially post-translationally modified 60 residue antimicrobial neuroendocrine peptide called chrombacin. The study was performed by high resolution FT MS using complementary fragmentation techniques. The peptide was analyzed at low levels...

journal_title：Journal of proteome research

authors： Taylor SW,Sun C,Hsieh A,Andon NL,Ghosh SS

更新日期：2008-02-01 00:00:00