Proteins associated with immunopurified granules from a model pancreatic islet beta-cell system: proteomic snapshot of an endocrine secretory granule.

abstract：:The identification of commercial shrimp species is a relevant issue to ensure correct labeling, maintain consumer confidence and enhance the knowledge of the captured species, benefiting both, fisheries and manufacturers. A proteomic approach, based on 2DE, tryptic in-gel digestion, MALDI-TOF MS, and ESI-MS/MS analyse...

journal_title：Journal of proteome research

authors： Ortea I,Cañas B,Gallardo JM

更新日期：2009-11-01 00:00:00

abstract：:The peptide toxins in the venoms of small invertebrates such as stinging ants have rarely been studied due to the limited amount of venom available per individual. We used a venomics strategy to identify the molecular diversity of the venom peptidome for the myrmicine ant Tetramorium bicarinatum. The methodology inclu...

journal_title：Journal of proteome research

authors： Touchard A,Téné N,Song PCT,Lefranc B,Leprince J,Treilhou M,Bonnafé E

更新日期：2018-10-05 00:00:00

abstract：:The Biology/Disease-driven (B/D) working groups of the Human Proteome Project are alliances of research groups aimed at developing or improving proteomic tools to support specific biological or disease-related research areas. Here, we describe the activities and progress to date of the B/D working group focused on pro...

journal_title：Journal of proteome research

authors： Boersema PJ,Melnik A,Hazenberg BPC,Rezeli M,Marko-Varga G,Kamiie J,Portelius E,Blennow K,Zubarev RA,Polymenidou M,Picotti P

更新日期：2018-12-07 00:00:00

abstract：:Antibody reagents have been remained as a standard approach to characterize blood group (BG) antigens in clinic. The specificity and cross-reactivity of these BG antibodies are routine detected using the gel microcolumn assay (GMA). However, the GMA is neither specific nor sensitive, thus increasing the risk of improp...

journal_title：Journal of proteome research

authors： Yang L,Yu Y,Ma C,Wang H,Dai J,Duan H,Fu Z,Wu P,Wang D,Yu X

更新日期：2018-09-07 00:00:00

abstract：:Glutaredoxins (GRXs), small redox proteins that use reduced glutathione as an electron donor, are key components of the cellular antioxidant system. In this study, we used Saccharomyces cerevisiae as a model system to investigate the effects of GRX deletion on yeast chronological life span (CLS). Deletion of either Gr...

journal_title：Journal of proteome research

authors： Liu Y,Yang F,Li S,Dai J,Deng H

更新日期：2018-07-06 00:00:00

abstract：:Methotrexate (MTX) is a chemotherapeutic agent that can cause a range of toxic side effects including gastrointestinal damage, hepatotoxicity, myelosuppression, and nephrotoxicity and has potentially complex interactions with the gut microbiome. Following untargeted UPLC-qtof-MS analysis of urine and fecal samples fro...

journal_title：Journal of proteome research

authors： Letertre MPM,Munjoma N,Wolfer K,Pechlivanis A,McDonald JAK,Hardwick RN,Cherrington NJ,Coen M,Nicholson JK,Hoyles L,Swann JR,Wilson ID

更新日期：2020-08-07 00:00:00

abstract：:Cystinuria is a purely renal, rare genetic disease caused by mutations in cystine transporter genes and characterized by defective cystine reabsorption leading to kidney stones. In 14% of cases, patients undergo nephrectomy, but given the difficulty to predict the evolution of the disease, the identification of marker...

journal_title：Journal of proteome research

authors： Bourderioux M,Nguyen-Khoa T,Chhuon C,Jeanson L,Tondelier D,Walczak M,Ollero M,Bekri S,Knebelmann B,Escudier E,Escudier B,Edelman A,Guerrera IC

更新日期：2015-01-02 00:00:00

abstract：:Roux-en-Y gastric bypass (RYGB) is one of the most effective treatments for long-term weight loss and diabetes remission; however, the mechanisms underlying these changes are not clearly understood. In this study, the serum metabolic profiles of 23 remission and 12 nonremission patients with type 2 diabetes mellitus (...

journal_title：Journal of proteome research

authors： Luo P,Yu H,Zhao X,Bao Y,Hong CS,Zhang P,Tu Y,Yin P,Gao P,Wei L,Zhuang Z,Jia W,Xu G

更新日期：2016-04-01 00:00:00

abstract：:Many pressing medical challenges, such as diagnosing disease, enhancing directed stem-cell differentiation, and classifying cancers, have long been hindered by limitations in our ability to quantify proteins in single cells. Mass spectrometry (MS) is poised to transcend these limitations by developing powerful methods...

journal_title：Journal of proteome research

authors： Specht H,Slavov N

更新日期：2018-08-03 00:00:00

abstract：:Human plasma and serum proteins are subject to intrinsic proteolytic degradation both during and after blood collection. By monitoring peptides, we investigated the stability of plasma and serum samples and the effects of anticoagulants and protease inhibitors on the plasma samples. Serum and plasma were subjected to ...

journal_title：Journal of proteome research

authors： Yi J,Kim C,Gelfand CA

更新日期：2007-05-01 00:00:00

abstract：:We report the comparative proteomic characterization of the venoms of adult and newborn specimens of the lancehead pitviper Bothrops asper from two geographically isolated populations from the Caribbean and the Pacific versants of Costa Rica. The crude venoms were fractionated by reverse-phase HPLC, followed by analys...

journal_title：Journal of proteome research

authors： Alape-Girón A,Sanz L,Escolano J,Flores-Díaz M,Madrigal M,Sasa M,Calvete JJ

更新日期：2008-08-01 00:00:00

abstract：:Cellular functions are largely carried out by noncovalent protein complexes that may exist within the cell as stable modules or as assemblies of dynamically changing composition, whose formation and decomposition are triggered in response to extracellular stimuli. The protein constituents of complexes often exhibit po...

journal_title：Journal of proteome research

authors： Przybylski C,Jünger MA,Aubertin J,Radvanyi F,Aebersold R,Pflieger D

更新日期：2010-10-01 00:00:00

abstract：:Human milk oligosaccharides (HMOs) perform a number of functions including serving as prebiotics to stimulate the growth of beneficial intestinal bacteria, as receptor analogues to inhibit binding of pathogens, and as substances that promote postnatal brain development. There is further evidence that HMOs participate ...

journal_title：Journal of proteome research

authors： Wu S,Tao N,German JB,Grimm R,Lebrilla CB

更新日期：2010-08-06 00:00:00

abstract：:FDA oversight of medical devices, including in vitro diagnostic devices (IVDs or laboratory tests), in the United States was a direct result of the passage of the Medical Device Amendments of 1976. This law introduced a series of general controls for medical devices including registration and listing, requirements for...

journal_title：Journal of proteome research

authors： Hackett JL,Gutman SI

更新日期：2005-07-01 00:00:00

abstract：:Knowledge of the protein networks interacting with the amyloid precursor protein (APP) in vivo can shed light on the physiological function of APP. To date, most proteins interacting with the APP intracellular domain (AICD) have been identified by Yeast Two Hybrid screens which only detect direct interaction partners....

journal_title：Journal of proteome research

authors： Kohli BM,Pflieger D,Mueller LN,Carbonetti G,Aebersold R,Nitsch RM,Konietzko U

更新日期：2012-08-03 00:00:00

abstract：:Mass-spectrometry-based proteomics has become the standard approach for identifying and quantifying proteins. A vital step consists of analyzing experimentally generated mass spectra to identify the underlying peptide sequences for later mapping to the originating proteins. We here present the latest developments in S...

journal_title：Journal of proteome research

authors： Barsnes H,Vaudel M

更新日期：2018-07-06 00:00:00

abstract：:Protein post-translational modification by ubiquitin-fold modifier 1, UFM1, regulates many biological processes such as response to endoplasmic reticulum stress and regulation of tumor progression. A recent study has indicated that the UFM1-binding and PCI domain-containing protein 1 (UFBP1) is required for the conjug...

journal_title：Journal of proteome research

authors： Zhu Y,Lei Q,Li D,Zhang Y,Jiang X,Hu Z,Xu G

更新日期：2018-04-06 00:00:00

abstract：:A role for Src Family Kinases (SFKs) in the dynamics of endocytic and secretory pathways has previously been reported. Identification of low-abundance compartmentalized complexes still remains challenging, highlighting the need for novel tools. Here we describe analysis of SFK-signaling complexes of hepatic Golgi/endo...

journal_title：Journal of proteome research

authors： Bilodeau N,Fiset A,Boulanger MC,Bhardwaj S,Winstall E,Lavoie JN,Faure RL

更新日期：2010-02-05 00:00:00

abstract：:Over the past few years, awareness has risen that for mass-spectrometry-based proteomics methods to mature into everyday analytical and clinical practices, extensive quality assessment is mandatory. A currently overlooked source of qualitative information originates from the mass spectrometer itself. Apart from the ac...

journal_title：Journal of proteome research

authors： Bittremieux W,Willems H,Kelchtermans P,Martens L,Laukens K,Valkenborg D

更新日期：2015-05-01 00:00:00

abstract：:In this review, we lead the reader through the evolution of proteomics application to the study of quality control in production processes of foods (including food of plant origin and transgenic plants in particular, but also meat, wine and beer, and milk) and food safety (screening for foodborne pathogens). These top...

journal_title：Journal of proteome research

authors： D'Alessandro A,Zolla L

更新日期：2012-01-01 00:00:00

abstract：:Human milk is a rich source of bioactive proteins that support the early growth and development of the newborn. Although the major components of the protein fraction in human milk have been studied, the expression and relative abundance of minor components have received limited attention. We examined the expression of...

journal_title：Journal of proteome research

authors： Liao Y,Alvarado R,Phinney B,Lönnerdal B

更新日期：2011-04-01 00:00:00

abstract：:Epithelial ovarian carcinoma has in general a poor prognosis since the vast majority of tumors are genomically unstable and clinically highly aggressive. This results in rapid progression of malignancy potential while still asymptomatic and thus in late diagnosis. It is therefore of critical importance to develop meth...

journal_title：Journal of proteome research

authors： Waldemarson S,Krogh M,Alaiya A,Kirik U,Schedvins K,Auer G,Hansson KM,Ossola R,Aebersold R,Lee H,Malmström J,James P

更新日期：2012-05-04 00:00:00

abstract：:We report the structural analysis of cap-binding proteins using a chemical probe/ultraviolet photodissociation (UVPD) mass spectrometry strategy for evaluating solvent accessibility of proteins. Our methodology utilized a chromogenic probe (NN) to probe the exposed amine residues of wheat eukaryotic translation initia...

journal_title：Journal of proteome research

authors： O'Brien JP,Mayberry LK,Murphy PA,Browning KS,Brodbelt JS

更新日期：2013-12-06 00:00:00

abstract：:Sexual dimorphism is initialed by the components of the sex determination pathway and is most evident in gonads and germ cells. Although striking dimorphic expressions have been detected at the transcriptional level between the silkworm larval testis and the ovary, the sex-dimorphic expressions at the protein level ha...

journal_title：Journal of proteome research

authors： Chen JE,Li JY,You ZY,Liu LL,Liang JS,Ma YY,Chen M,Zhang HR,Jiang ZD,Zhong BX

更新日期：2013-06-07 00:00:00

abstract：:The gut microbiota plays a critical role in the pathogenesis of nonalcoholic fatty liver disease (NAFLD). Increased fructose consumption and inadequate copper intake are two critical risk factors in the development of NAFLD. To gain insight into the role of gut microbiota, fecal metabolites, obtained from rats exposed...

journal_title：Journal of proteome research

authors： Wei X,Song M,Yin X,Schuschke DA,Koo I,McClain CJ,Zhang X

更新日期：2015-09-04 00:00:00

abstract：:We propose a method for finding features in liquid chromatography mass spectrometry data that is based on the isotopic pattern of peaks. Our interactive approach to feature finding is carried out across many samples simultaneously and aligns features concurrently. Our scale-independent approach prioritises potential f...

journal_title：Journal of proteome research

authors： Clifford D,Buckley M,Fung KY,Cosgrove L

更新日期：2006-11-01 00:00:00

abstract：:The purpose of this discovery phase study was to identify candidate protein biomarkers for high-grade dysplastic cervical cells using mass spectrometry. Laser Capture Microdissection (LCM) was utilized to isolate high-grade dysplastic and normal cells from ThinPrep slides prepared from cervical cytological specimens. ...

journal_title：Journal of proteome research

authors： Gu Y,Wu SL,Meyer JL,Hancock WS,Burg LJ,Linder J,Hanlon DW,Karger BL

更新日期：2007-11-01 00:00:00

abstract：:Fusarium graminearum (FG) is a serious plant pathogen causing huge losses in global production of wheat and other cereals. Tri5-gene encoded trichodiene synthase is the first key enzyme for biosynthesis of trichothecene mycotoxins in FG. To further our understandings of FG metabolism which is essential for developing ...

journal_title：Journal of proteome research

authors： Chen F,Zhang J,Song X,Yang J,Li H,Tang H,Liao YC

更新日期：2011-05-06 00:00:00

abstract：:"Dry eye" is a multifactorial inflammatory disease affecting the ocular surface. Tear hyperosmolarity in dry eye contributes to inflammation and cell damage. Recent research efforts on dry eye have been directed toward biomarker discovery for diagnosis, response to treatment, and disease mechanisms. This study employe...

journal_title：Journal of proteome research

authors： Chen L,Li J,Guo T,Ghosh S,Koh SK,Tian D,Zhang L,Jia D,Beuerman RW,Aebersold R,Chan EC,Zhou L

更新日期：2015-09-04 00:00:00

abstract：:Severe acute pancreatitis (SAP) is caused by complicated biological factors, and revealing its complex pathogenesis by single-target analysis is difficult. Systematic studies have developed slowly because extraction of degradable pancreatic proteins exposed to multiple proteases is challenging. We present integrated w...

journal_title：Journal of proteome research

authors： Wang C,Zhang Y,Tan J,Chen B,Sun L

更新日期：2020-06-05 00:00:00