Proteins associated with immunopurified granules from a model pancreatic islet beta-cell system: proteomic snapshot of an endocrine secretory granule.

Abstract:

:beta-Cell granules contain proteins involved in fuel regulation, which when altered, contribute to metabolic disorders including diabetes mellitus. We analyzed proteins present in purified granules from the INS-1E beta-cell model. Fifty-one component proteins were identified by LC-MS/MS including hormones, granins, protein processing components, cellular trafficking components, enzymes implicated in cellular metabolism and chaperone proteins. These findings may increase understanding of granule secretion and the processes leading to protein aggregation and beta-cell death in type-2 diabetes.

journal_name

J Proteome Res

authors

Hickey AJ,Bradley JW,Skea GL,Middleditch MJ,Buchanan CM,Phillips AR,Cooper GJ

doi

10.1021/pr800675k

subject

Has Abstract

pub_date

2009-01-01 00:00:00

pages

178-86

issue

1

eissn

1535-3893

issn

1535-3907

pii

10.1021/pr800675k

journal_volume

8

pub_type

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