Abstract:
:Severe acute pancreatitis (SAP) is caused by complicated biological factors, and revealing its complex pathogenesis by single-target analysis is difficult. Systematic studies have developed slowly because extraction of degradable pancreatic proteins exposed to multiple proteases is challenging. We present integrated whole proteomic and phosphoproteomic profiles of SAP rats based on a modified protein extraction strategy with less protein degradation. Data-dependent acquisition (DDA) and data-independent acquisition (DIA) strategies were applied to select an appropriate method. Total 275 differentially expressed proteins and 757 differentially expressed phosphorylated proteins were identified by DIA-based quantitative proteomics. Several signal transduction pathways, including the AMPK, MAPK, and PI3K-Akt pathways, were enriched in SAP. Up-regulation of phosphorylated proteins involved in the process of TNFA signaling and inflammatory response was also detected in SAP. Our results improve the understanding of SAP development and progression.
journal_name
J Proteome Resjournal_title
Journal of proteome researchauthors
Wang C,Zhang Y,Tan J,Chen B,Sun Ldoi
10.1021/acs.jproteome.0c00229subject
Has Abstractpub_date
2020-06-05 00:00:00pages
2471-2482issue
6eissn
1535-3893issn
1535-3907journal_volume
19pub_type
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