Profiling of glycans in serum for the discovery of potential biomarkers for ovarian cancer.

abstract：:Proteins are involved in all tasks of life, and their characterization is essential to understand the underlying mechanisms of biological processes. We present a method called "differential visual proteomics" geared to study proteome-wide structural changes of proteins and protein-complexes between a disturbed and an ...

journal_title：Journal of proteome research

authors： Syntychaki A,Rima L,Schmidli C,Stohler T,Bieri A,Sütterlin R,Stahlberg H,Castaño-Díez D,Braun T

更新日期：2019-09-06 00:00:00

abstract：:Protein microarrays consist of known proteins spotted onto solid substrates and are used to perform highly multivariate assessments of protein-binding interactions. Human protein arrays are routinely applied to pathogen detection, immune response biomarker profiling, and antibody specificity profiling. Here, we descri...

journal_title：Journal of proteome research

authors： Feng F,Ataca ST,Ran M,Wang Y,Breen M,Kepler TB

更新日期：2020-07-02 00:00:00

abstract：:Royal jelly (RJ) is an exclusive food for queen honey bee (Apis mellifera L.) that is synthesized and secreted by young worker bees. RJ is also widely used in medical products, cosmetics, and as health foods. However, little is known about RJ functionality and the total protein components, although recent research is ...

journal_title：Journal of proteome research

authors： Furusawa T,Rakwal R,Nam HW,Shibato J,Agrawal GK,Kim YS,Ogawa Y,Yoshida Y,Kouzuma Y,Masuo Y,Yonekura M

更新日期：2008-08-01 00:00:00

abstract：:Bacillus Amyloliquefaciens SQR9 is a well-investigated plant growth-promoting rhizobacteria with strong root colonization capability. To identify the key proteins involved in in situ root colonization and biofilm formation, the proteomic profiles of planktonic and root colonized SQR9 cells were compared. A total of 75...

journal_title：Journal of proteome research

authors： Qiu M,Xu Z,Li X,Li Q,Zhang N,Shen Q,Zhang R

更新日期：2014-12-05 00:00:00

abstract：:Adipose tissue is a major storage site for lipophilic environmental contaminants. The environmental metabolic disruptor hypothesis postulates that some pollutants can promote obesity or metabolic disorders by activating nuclear receptors involved in the control of energetic homeostasis. In this context, monoethylhexyl...

journal_title：Journal of proteome research

authors： Ellero-Simatos S,Claus SP,Benelli C,Forest C,Letourneur F,Cagnard N,Beaune PH,de Waziers I

更新日期：2011-12-02 00:00:00

abstract：:Detergents are indispensable solubilizing agents in the purification and analysis of membrane proteins. For mass spectrometric identification of proteins, it is essential that detergents are removed prior to analysis, necessitating an in-gel digestion step. Here, we report a procedure that allows use of detergents and...

journal_title：Journal of proteome research

authors： Nagaraj N,Lu A,Mann M,Wiśniewski JR

更新日期：2008-11-01 00:00:00

abstract：:The development of medical interventions for the preservation of disease-free longevity would be facilitated by markers that predict healthy aging. Altered protein N-glycosylation patterns have been found with increasing age and several disease states. Here we investigate whether glycans derived from the total glycopr...

journal_title：Journal of proteome research

authors： Ruhaak LR,Uh HW,Beekman M,Hokke CH,Westendorp RG,Houwing-Duistermaat J,Wuhrer M,Deelder AM,Slagboom PE

更新日期：2011-04-01 00:00:00

abstract：:Hepatocellular carcinoma (HCC) remains as the fifth most common cancer in the world and accounts for more than 700,000 deaths annually. Changes in serum glycosylation have long been associated with this cancer but the source of that material is unknown and direct glycan analysis of HCC tissues has been limited. Our la...

journal_title：Journal of proteome research

authors： West CA,Wang M,Herrera H,Liang H,Black A,Angel PM,Drake RR,Mehta AS

更新日期：2018-10-05 00:00:00

abstract：:Salt stress is a major abiotic factor limiting maize yield. To characterize the mechanism underlying maize salt tolerance, we compared the seedling root proteomes of salt-tolerant Jing724 and salt-sensitive D9H. The germination rate and growth parameter values (weight and length) were higher for Jing724 than for D9H u...

journal_title：Journal of proteome research

authors： Luo M,Zhao Y,Wang Y,Shi Z,Zhang P,Zhang Y,Song W,Zhao J

更新日期：2018-01-05 00:00:00

abstract：:While the proteome defines the expressed gene products, the metabolome results from reactions controlled by such gene products. Plasma represents an accessible "window" to the metabolome both in regard of availability and content. The wide range of the plasma metabolome, in terms of molecular diversity and abundance, ...

journal_title：Journal of proteome research

authors： Griffiths WJ,Hornshaw M,Woffendin G,Baker SF,Lockhart A,Heidelberger S,Gustafsson M,Sjövall J,Wang Y

更新日期：2008-08-01 00:00:00

abstract：:A number of aptamers have been selected against cell surface biomarkers or against eukaryotic tissue culture cells themselves. To determine the general utility of aptamers for assessing the cell surface proteome, we developed a standardized flow cytometry assay and carried out a comprehensive study with 7 different ap...

journal_title：Journal of proteome research

authors： Li N,Ebright JN,Stovall GM,Chen X,Nguyen HH,Singh A,Syrett A,Ellington AD

更新日期：2009-05-01 00:00:00

abstract：:Ovarian cancer remains a deadly threat to women as the disease is often diagnosed in the late stages when the chance of survival is low. There are no good biomarkers available for early detection and only a few markers have shown clinical utility for prognosis, response to therapy and disease recurrence. We mined cond...

journal_title：Journal of proteome research

authors： Gunawardana CG,Kuk C,Smith CR,Batruch I,Soosaipillai A,Diamandis EP

更新日期：2009-10-01 00:00:00

abstract：:Porcine reproductive and respiratory syndrome (PRRS) has devastated the pig industry worldwide for almost 25 years, and its virus (PRRSV) preferentially infects and replicates in pulmonary alveolar macrophages (PAMs). To discover cellular protein responses in PRRSV-infected PAMs, two-dimensional liquid chromatography-...

journal_title：Journal of proteome research

authors： Lu Q,Bai J,Zhang L,Liu J,Jiang Z,Michal JJ,He Q,Jiang P

更新日期：2012-05-04 00:00:00

abstract：:Recent progress in genomics, proteomics, and bioinformatics enables unprecedented opportunities to examine the evolutionary history of molecular, cellular, and developmental pathways through phylogenomics. Accordingly, we have developed a motif analysis tool for phylogenomics (Phylomat, http://alg.ncsa.uiuc.edu/pmat) ...

journal_title：Journal of proteome research

authors： Graham WV,Tcheng DK,Shirk AL,Attene-Ramos MS,Welge ME,Gaskins HR

更新日期：2004-11-01 00:00:00

abstract：:An unusual sulfotyrosine-, phosphoserine-containing motif was mapped on a differentially post-translationally modified 60 residue antimicrobial neuroendocrine peptide called chrombacin. The study was performed by high resolution FT MS using complementary fragmentation techniques. The peptide was analyzed at low levels...

journal_title：Journal of proteome research

authors： Taylor SW,Sun C,Hsieh A,Andon NL,Ghosh SS

更新日期：2008-02-01 00:00:00

abstract：:Cannabinoid 2 receptor (CB2R), a Class-A G-protein coupled receptor (GPCR), is a promising drug target under a wide array of pathological conditions. Rational drug design has been hindered due to our poor understanding of the structural features involved in ligand binding. Binding of a high-affinity biarylpyrazole inv...

journal_title：Journal of proteome research

authors： Mallipeddi S,Kreimer S,Zvonok N,Vemuri K,Karger BL,Ivanov AR,Makriyannis A

更新日期：2017-07-07 00:00:00

abstract：:In shotgun proteomics analysis, user-specified parameters are critical to database search performance and therefore to the yield of confident peptide-spectrum matches (PSMs). Two of the most important parameters are related to the accuracy of the mass spectrometer. Precursor mass tolerance defines the peptide candidat...

journal_title：Journal of proteome research

authors： May DH,Tamura K,Noble WS

更新日期：2017-04-07 00:00:00

abstract：:A popular method for peptide quantification relies on isobaric labeling such as tandem mass tags (TMT), which enables multiplexed proteome analyses. Quantification is achieved by reporter ions generated by fragmentation in a tandem mass spectrometer. However, with higher degrees of multiplexing, the smaller mass diffe...

journal_title：Journal of proteome research

authors： Kelstrup CD,Aizikov K,Batth TS,Kreutzman A,Grinfeld D,Lange O,Mourad D,Makarov AA,Olsen JV

更新日期：2018-11-02 00:00:00

abstract：:Ozone (O3), a potent air pollutant and a significant greenhouse gas, has been recognized as a major component of global climate change. However, current increasing trends in its background level are projecting a more severe threat to natural and cultivated plants in the near future. The present study has been designed...

journal_title：Journal of proteome research

authors： Sarkar A,Rakwal R,Bhushan Agrawal S,Shibato J,Ogawa Y,Yoshida Y,Kumar Agrawal G,Agrawal M

更新日期：2010-09-03 00:00:00

abstract：:Pathogenic events in Alzheimer's disease are believed to involve an imbalance between the production and clearance of the neurotoxic 42 amino acid form of the beta-amyloid peptide (Abeta1-42). Although much is known about the production of Abeta1-42, many questions remain about its degradation. Here, we describe an op...

journal_title：Journal of proteome research

authors： Portelius E,Tran AJ,Andreasson U,Persson R,Brinkmalm G,Zetterberg H,Blennow K,Westman-Brinkmalm A

更新日期：2007-11-01 00:00:00

abstract：:Liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based proteomic methods have been widely used to identify lysine acylation proteins. However, these experimental approaches often fail to detect proteins that are in low abundance or absent in specific biological samples. To circumvent these problems, we develo...

journal_title：Journal of proteome research

authors： Du Y,Zhai Z,Li Y,Lu M,Cai T,Zhou B,Huang L,Wei T,Li T

更新日期：2016-12-02 00:00:00

abstract：:Mycobacterium tuberculosis, the causative agent of tuberculosis, accounts for 1.5 million human deaths annually worldwide. Despite efforts to eradicate tuberculosis, it still remains a deadly disease. The two best characterized strains of M. tuberculosis, virulent H37Rv and avirulent H37Ra, provide a unique platform t...

journal_title：Journal of proteome research

authors： Verma R,Pinto SM,Patil AH,Advani J,Subba P,Kumar M,Sharma J,Dey G,Ravikumar R,Buggi S,Satishchandra P,Sharma K,Suar M,Tripathy SP,Chauhan DS,Gowda H,Pandey A,Gandotra S,Prasad TS

更新日期：2017-04-07 00:00:00

abstract：:As a potential tool for proteomics and protein characterization, in-gel cysteine- and arginine-specific cleavage is demonstrated by means of trypsin or endoproteinase Lys-C for six model proteins (lysozyme, alpha-lactalbumin, beta-lactoglobulin, ribonuclease A, albumin, and transferrin), ranging in size from 14 kDa to...

journal_title：Journal of proteome research

authors： Thevis M,Ogorzalek Loo RR,Loo JA

更新日期：2003-03-01 00:00:00

abstract：:A detailed understanding of the relationships between the distinct metabolic compartments of blood and milk would be of potential benefit to our understanding of the physiology of lactation, and potentially for development of biomarkers for health and commercially relevant traits in dairy cattle. NMR methods were used...

journal_title：Journal of proteome research

authors： Maher AD,Hayes B,Cocks B,Marett L,Wales WJ,Rochfort SJ

更新日期：2013-03-01 00:00:00

abstract：:Spectral libraries provide a sensitive and accurate method for identifying peptides from tandem mass spectra, complementary to searching genome-derived databases or sequencing de novo. Their application requires comprehensive libraries including peptides from low-abundant proteins. Here we describe a method for constr...

journal_title：Journal of proteome research

authors： van der Plas-Duivesteijn SJ,Mohammed Y,Dalebout H,Meijer A,Botermans A,Hoogendijk JL,Henneman AA,Deelder AM,Spaink HP,Palmblad M

更新日期：2014-03-07 00:00:00

abstract：:Gas chromatography-mass spectrometry (GC-MS) platforms are typically run in electron ionization (EI) mode for mass spectral matching and metabolite annotation. With the advent of high resolution mass spectrometry (HRMS), soft ionization techniques such as chemical ionization (CI) may provide additional coverage for co...

journal_title：Journal of proteome research

authors： Misra BB,Olivier M

更新日期：2020-07-02 00:00:00

abstract：:Proteolysis of autoantigens can alter normal MHC class II antigen processing and has been implicated in the induction of autoimmune diseases. Many autoantigens are substrates for the protease granzyme B (GrB), but the mechanistic significance of this association is unknown. Peptidylarginine deiminase 4 (PAD4) is a fre...

journal_title：Journal of proteome research

authors： Darrah E,Kim A,Zhang X,Boronina T,Cole RN,Fava A,Giles JT,Bingham III CO,Chalmers MJ,Griffin PR,Sadegh-Nasseri S,Rosen A

更新日期：2017-01-06 00:00:00

abstract：:Hepatocellular carcinoma is one of the most fatal cancers worldwide. In this study, a reversed-phase-strong cation exchange-reversed-phase three-dimensional liquid chromatography strategy was established and coupled with mass spectrometry to investigate the differential proteome expression of HCC and normal liver tiss...

journal_title：Journal of proteome research

authors： Xu B,Wang F,Song C,Sun Z,Cheng K,Tan Y,Wang H,Zou H

更新日期：2014-08-01 00:00:00

abstract：:Bacillus weihenstephanensis is a subspecies of the Bacillus cereus sensu lato group of spore-forming bacteria known to cause food spoilage or food poisoning. The key distinguishing phenotype of B. weihenstephanensis is its ability to grow below 7 °C or, from a food safety perspective, to grow and potentially produce t...

journal_title：Journal of proteome research

authors： Stelder SK,Mahmud SA,Dekker HL,de Koning LJ,Brul S,de Koster CG

更新日期：2015-05-01 00:00:00

abstract：:We have investigated the molecular mechanisms regulating the necrosis and apoptosis that occur on treatment of mouse mammary tumor FM3A cells with 5-fluoro-2'-deoxyuridine (FUdR), a potent anticancer agent, using the original clone F28-7 and its variant F28-7-A cells. Previously, we reported an interesting observation...

journal_title：Journal of proteome research

authors： Sato A,Satake A,Hiramoto A,Wataya Y,Kim HS

更新日期：2010-05-07 00:00:00