Abstract：
:To decrease the mortality of lung cancer, better screening and diagnostic tools as well as treatment options are needed. Protein glycosylation is one of the major post-translational modifications that is altered in cancer, but it is not exactly clear which glycan structures are affected. A better understanding of the glycan structures that are differentially regulated in lung tumor tissue is highly desirable and will allow us to gain greater insight into the underlying biological mechanisms of aberrant glycosylation in lung cancer. Here, we assess differential glycosylation patterns of lung tumor tissue and nonmalignant tissue at the level of individual glycan structures using nLC-chip-TOF-MS. Using tissue samples from 42 lung adenocarcinoma patients, 29 differentially expressed (FDR < 0.05) glycan structures were identified. The levels of several oligomannose type glycans were upregulated in tumor tissue. Furthermore, levels of fully galactosylated glycans, some of which were of the hybrid type and mostly without fucose, were decreased in cancerous tissue, whereas levels of non- or low-galactosylated glycans mostly with fucose were increased. To further assess the regulation of the altered glycosylation, the glycomics data was compared to publicly available gene expression data from lung adenocarcinoma tissue compared to nonmalignant lung tissue. The results are consistent with the possibility that the observed N-glycan changes have their origin in differentially expressed glycosyltransferases. These results will be used as a starting point for the further development of clinical glycan applications in the fields of imaging, drug targeting, and biomarkers for lung cancer.
journal_name
J Proteome Resjournal_title
Journal of proteome researchauthors
Ruhaak LR,Taylor SL,Stroble C,Nguyen UT,Parker EA,Song T,Lebrilla CB,Rom WN,Pass H,Kim K,Kelly K,Miyamoto Sdoi
10.1021/acs.jproteome.5b00255subject
Has Abstractpub_date
2015-11-06 00:00:00pages
4538-49issue
11eissn
1535-3893issn
1535-3907journal_volume
14pub_type
杂志文章abstract：:Recently, we reported comparative analysis of glycoproteins which express cancer-specific N-glycans on various cancer cells and identified 24 glycoproteins having polylactosamine (polyLacNAc)-type N-glycans that are abundantly present in malignant cells [ Mitsui et al., J. Pharm. Biomed. Anal. 2012 , 70 , 718 - 726 ]....
journal_title：Journal of proteome research
pub_type： 杂志文章
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journal_title：Journal of proteome research
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journal_title：Journal of proteome research
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journal_title：Journal of proteome research
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journal_title：Journal of proteome research
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更新日期：2016-08-05 00:00:00
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更新日期：2014-12-05 00:00:00
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更新日期：2013-09-06 00:00:00
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journal_title：Journal of proteome research
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doi：10.1021/pr500484w
更新日期：2014-11-07 00:00:00
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pub_type： 杂志文章
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更新日期：2013-01-04 00:00:00
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journal_title：Journal of proteome research
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doi：10.1021/acs.jproteome.8b00227
更新日期：2018-07-06 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/acs.jproteome.6b01026
更新日期：2017-08-04 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/acs.jproteome.7b00843
更新日期：2018-04-06 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr801114k
更新日期：2009-09-01 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章,评审
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更新日期：2005-07-01 00:00:00
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journal_title：Journal of proteome research
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更新日期：2012-02-03 00:00:00
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journal_title：Journal of proteome research
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journal_title：Journal of proteome research
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更新日期：2012-10-05 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章,评审
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更新日期：2019-06-07 00:00:00
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更新日期：2011-04-01 00:00:00
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更新日期：2013-02-01 00:00:00
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更新日期：2014-08-01 00:00:00
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更新日期：2008-04-01 00:00:00
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journal_title：Journal of proteome research
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更新日期：2007-05-01 00:00:00
abstract：:Natural killer (NK) cells are important components of innate immune defense. NK cells kill virus-infected cells and secrete cytokines that are involved in activation of other immune cells. Macrophage-derived cytokines interferon-alpha (IFN-alpha) and interleukin-15 (IL-15) are in turn important activators of NK cells,...
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更新日期：2005-01-01 00:00:00
