Abstract:
:We have developed a high-throughput system for generating baculoviruses and testing the expression, solubility, and affinity column purification of encoded proteins. We have used this system to generate baculoviruses for and analyze the expression of 337 proteins from three different herpesviruses (HSV-1, EBV, and CMV) and vaccinia virus. Subsets of these proteins were also tested for expression and solubility in E. coli. Comparisons of the results in the two systems are presented for each virus.
journal_name
J Proteome Resjournal_title
Journal of proteome researchauthors
Gao M,Brufatto N,Chen T,Murley LL,Thalakada R,Domagala M,Beattie B,Mamelak D,Athanasopoulos V,Johnson D,McFadden G,Burks C,Frappier Ldoi
10.1021/pr050137ukeywords:
subject
Has Abstractpub_date
2005-11-01 00:00:00pages
2225-35issue
6eissn
1535-3893issn
1535-3907journal_volume
4pub_type
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