Online microwave D-cleavage LC-ESI-MS/MS of intact proteins: site-specific cleavages at aspartic acid residues and disulfide bonds.

abstract：:Histones are subjected to extensive post-translational modifications (PTMs) that are known to play key roles in many biological processes. In this study, we report a fast, efficient, highly reproducible, and easily automated method involving ultra-high performance liquid chromatography (UHPLC) coupled to a high resolu...

journal_title：Journal of proteome research

authors： Contrepois K,Ezan E,Mann C,Fenaille F

更新日期：2010-10-01 00:00:00

abstract：:Tropomyosins (Tm) constitute a family of ubiquitous and highly conserved actin-binding proteins, playing essential roles in a variety of biological processes. Tm isoforms produced by multiple Tm encoding genes and alternatively expressed exons along with post-translational modifications (PTMs) regulate Tm function. Th...

journal_title：Journal of proteome research

authors： Peng Y,Chen X,Zhang H,Xu Q,Hacker TA,Ge Y

更新日期：2013-01-04 00:00:00

abstract：:Automated sequencing of unknowns in bottom-up proteomics makes the data produced susceptible to process control errors, which can be propagated into mistakes in analyte identification. Inclusion of an unintrusive internal standard, such as lysozyme, allows monitoring all phases of the proteomics process including samp...

journal_title：Journal of proteome research

authors： Riter LS,Hodge BD,Gooding KM,Julian RK Jr

更新日期：2005-01-01 00:00:00

abstract：:Hepatocellular carcinoma (HCC) is a major liver malignancy possessing a high mortality rate and is particularly prevalent in China and Asia. While surgery is the most effective treatment for liver tumor, about 80% of HCC patients are inoperable at presentation and die early due to late diagnosis. For early cancer dete...

journal_title：Journal of proteome research

authors： Sun S,Xu MZ,Poon RT,Day PJ,Luk JM

更新日期：2010-01-01 00:00:00

abstract：:The present study was undertaken to identify proteins interacting with PrP(C) that could provide new insights into its physiological functions and pathological role. Human PrP(C) was expressed in prion protein-deficient murine hippocampus (HpL3-4) neuronal cells. The PrP(C) along with its interacting proteins were aff...

journal_title：Journal of proteome research

authors： Zafar S,von Ahsen N,Oellerich M,Zerr I,Schulz-Schaeffer WJ,Armstrong VW,Asif AR

更新日期：2011-07-01 00:00:00

abstract：:"Omics" studies reported to date have dealt with either thoroughly characterized single species or poorly explored meta-microbial communities. However, these techniques are capable of producing highly informative data for the analysis of interactions between two organisms. We examined the bacterial interaction between...

journal_title：Journal of proteome research

authors： Nakanishi Y,Fukuda S,Chikayama E,Kimura Y,Ohno H,Kikuchi J

更新日期：2011-02-04 00:00:00

abstract：:The purpose of this study was to evaluate the use of high resolution LC-MS together with metabolomics and D(4)-cholic acid (D(4)-CA) as a metabolic tracer to measure the metabolism and reconjugation of bile acids (BAs) in vitro and in vivo. Metabolic tracers are very important because they allow for the direct detecti...

journal_title：Journal of proteome research

authors： Castro-Perez JM,Roddy TP,Shah V,Wang SP,Ouyang X,Ogawa A,McLaren DG,Tadin-Strapps M,Robinson MJ,Bartz SR,Ason B,Chen Y,Previs SF,Wong KK,Vreeken RJ,Johns DG,Hubbard BK,Hankemeier T,Mitnaul L

更新日期：2011-10-07 00:00:00

abstract：:Hypoxia is present in most solid tumors and is clinically correlated with increased metastasis and poor patient survival. While studies have demonstrated the role of hypoxia and hypoxia-regulated proteins in cancer progression, no attempts have been made to identify hypoxia-regulated proteins using quantitative proteo...

journal_title：Journal of proteome research

authors： Djidja MC,Chang J,Hadjiprocopis A,Schmich F,Sinclair J,Mršnik M,Schoof EM,Barker HE,Linding R,Jørgensen C,Erler JT

更新日期：2014-05-02 00:00:00

abstract：:The two key steps for analyzing proteomic data generated by high-resolution MS are database searching and postprocessing. While the two steps are interrelated, studies on their combinatory effects and the optimization of these procedures have not been adequately conducted. Here, we investigated the performance of thre...

journal_title：Journal of proteome research

authors： Tu C,Sheng Q,Li J,Ma D,Shen X,Wang X,Shyr Y,Yi Z,Qu J

更新日期：2015-11-06 00:00:00

abstract：:We have investigated the molecular mechanisms regulating the necrosis and apoptosis that occur on treatment of mouse mammary tumor FM3A cells with 5-fluoro-2'-deoxyuridine (FUdR), a potent anticancer agent, using the original clone F28-7 and its variant F28-7-A cells. Previously, we reported an interesting observation...

journal_title：Journal of proteome research

authors： Sato A,Satake A,Hiramoto A,Wataya Y,Kim HS

更新日期：2010-05-07 00:00:00

abstract：:Beta-thalassemia results from mutations of the β-hemoglobin (Hbb) gene and reduced functional Hbb synthesis. Excess α-Hb causes globin chain aggregation, oxidation, cytoskeletal damage, and increased red blood cell clearance. These events result in anemia, altered iron homeostasis, and expansion of extramedullary eryt...

journal_title：Journal of proteome research

authors： Vallelian F,Gelderman-Fuhrmann MP,Schaer CA,Puglia M,Opitz L,Baek JH,Vostal J,Buehler PW,Schaer DJ

更新日期：2015-02-06 00:00:00

abstract：:In this study, we aim to identify the potential biomarkers in hTG pathogenesis in schisandrin B-induced hTG mouse model. To investigate whether these identified biomarkers are only specific to schisandrin B-induced hTG mouse model, we also measured these biomarkers in a high fat diet (HFD)-induced hTG mouse model. We ...

journal_title：Journal of proteome research

authors： Kwan HY,Hu YM,Chan CL,Cao HH,Cheng CY,Pan SY,Tse KW,Wu YC,Yu ZL,Fong WF

更新日期：2013-03-01 00:00:00

abstract：:We demonstrate an approach for global quantitative analysis of protein mixtures using differential stable isotopic labeling of the enzyme-digested peptides combined with microbore liquid chromatography (LC) matrix-assisted laser desorption ionization (MALDI) mass spectrometry (MS). Microbore LC provides higher sample ...

journal_title：Journal of proteome research

authors： Ji C,Li L

更新日期：2005-05-01 00:00:00

abstract：:In an attempt to identify prostate cancer biomarkers with greater diagnostic and prognostic capabilities, we have developed an integrative proteomic discovery workflow focused on N-linked glycoproteins that refines the target selection process. In this work, hydrazide-based chemistry was used to identify N-linked glyc...

journal_title：Journal of proteome research

authors： Whitmore TE,Peterson A,Holzman T,Eastham A,Amon L,McIntosh M,Ozinsky A,Nelson PS,Martin DB

更新日期：2012-05-04 00:00:00

abstract：:Understanding how bacteria survive periods of cell wall stress is of fundamental interest and can help generate ideas for improved antibacterial treatments. In this study we use tandem mass tagging to characterize the proteomic response of vancomycin resistant Streptomyces coelicolor to the exposure to sublethal level...

journal_title：Journal of proteome research

authors： Hesketh A,Deery MJ,Hong HJ

更新日期：2015-07-02 00:00:00

abstract：:Ureteral obstruction will lead clinically to hydronephrosis, which may further develop into partial or complete loss of kidney function and even cause permanent histological damage. However, there is little knowledge of metabolic responses during the obstructed process and its recoverability. In this study, a complete...

journal_title：Journal of proteome research

authors： Wang Z,Xu R,Shen G,Feng J

更新日期：2018-09-07 00:00:00

abstract：:ProXL is a Web application and accompanying database designed for sharing, visualizing, and analyzing bottom-up protein cross-linking mass spectrometry data with an emphasis on structural analysis and quality control. ProXL is designed to be independent of any particular software pipeline. The import process is simpli...

journal_title：Journal of proteome research

authors： Riffle M,Jaschob D,Zelter A,Davis TN

更新日期：2016-08-05 00:00:00

abstract：:Clostridium difficile in recent years has undergone rapid evolution and has emerged as a serious human pathogen. Proteomic approaches can improve the understanding of the diversity of this important pathogen, especially in comparing the adaptive ability of different C. difficile strains. In this study, TMT labeling an...

journal_title：Journal of proteome research

authors： Chen JW,Scaria J,Mao C,Sobral B,Zhang S,Lawley T,Chang YF

更新日期：2013-03-01 00:00:00

abstract：:Knowledge of protein subcellular localization assists in the elucidation of protein function and understanding of different biological mechanisms that occur at discrete subcellular niches. Organelle-centric proteomics enables localization of thousands of proteins simultaneously. Although such techniques have successfu...

journal_title：Journal of proteome research

authors： Groen AJ,Sancho-Andrés G,Breckels LM,Gatto L,Aniento F,Lilley KS

更新日期：2014-02-07 00:00:00

abstract：:The plasma of patients with stable carotid atherosclerosis (n = 9), and healthy subjects (n = 10) have been fingerprinted with both GC-MS and (1)H NMR. Principal component analysis (PCA), partial least-squares-discriminant analysis (PLS-DA) and orthogonal partial least-squares-discriminant analysis (OPLS-DA) have been...

journal_title：Journal of proteome research

authors： Teul J,Rupérez FJ,Garcia A,Vaysse J,Balayssac S,Gilard V,Malet-Martino M,Martin-Ventura JL,Blanco-Colio LM,Tuñón J,Egido J,Barbas C

更新日期：2009-12-01 00:00:00

abstract：:Broadly neutralizing antibodies (bNAbs) that target the trimeric HIV-1 envelope glycoprotein spike (Env) are tools that can guide the design of recombinant Env proteins intended to engage the predicted human germline precursors of bNAbs (gl-bNAbs). The protein components of gl-bNAb epitopes are often masked by glycans...

journal_title：Journal of proteome research

authors： Behrens AJ,Kumar A,Medina-Ramirez M,Cupo A,Marshall K,Cruz Portillo VM,Harvey DJ,Ozorowski G,Zitzmann N,Wilson IA,Ward AB,Struwe WB,Moore JP,Sanders RW,Crispin M

更新日期：2018-03-02 00:00:00

abstract：:To decrease the mortality of lung cancer, better screening and diagnostic tools as well as treatment options are needed. Protein glycosylation is one of the major post-translational modifications that is altered in cancer, but it is not exactly clear which glycan structures are affected. A better understanding of the ...

journal_title：Journal of proteome research

authors： Ruhaak LR,Taylor SL,Stroble C,Nguyen UT,Parker EA,Song T,Lebrilla CB,Rom WN,Pass H,Kim K,Kelly K,Miyamoto S

更新日期：2015-11-06 00:00:00

abstract：:Propranolol is a nonselective beta-blocker of the beta-adrenergic receptors, and the S-enantiomer is more active compared with the R-enantiomer. Clinically, it has been shown to be effective in hypermetabolic burn patients by decreasing cardiac work, protein catabolism, and lipolysis. While gene expression profiles ha...

journal_title：Journal of proteome research

authors： Sui J,Tan TL,Zhang J,Ching CB,Chen WN

更新日期：2007-05-01 00:00:00

abstract：:The increasing occurrence of multidrug resistant tuberculosis exerts a major burden on treatment of this infectious disease. Thioridazine, previously used as a neuroleptic, is active against extensively drug resistant tuberculosis when added to other second- and third-line antibiotics. By quantitatively studying the p...

journal_title：Journal of proteome research

authors： de Keijzer J,Mulder A,de Haas PE,de Ru AH,Heerkens EM,Amaral L,van Soolingen D,van Veelen PA

更新日期：2016-06-03 00:00:00

abstract：:Stroke remains a major public health problem worldwide; it causes severe disability and is associated with high mortality rates. However, early diagnosis of stroke is difficult, and no reliable biomarkers are currently established. In this study, mass-spectrometry-based metabolomics was utilized to characterize the me...

journal_title：Journal of proteome research

authors： Liu P,Li R,Antonov AA,Wang L,Li W,Hua Y,Guo H,Wang L,Liu P,Chen L,Tian Y,Xu F,Zhang Z,Zhu Y,Huang Y

更新日期：2017-02-03 00:00:00

abstract：:Nostoc punctiforme ATCC 29133 is a photoautotrophic cyanobacterium with the capacity to fix atmospheric N 2. Its ability to mediate this process is similar to that described for Nostoc sp. PCC 7120, where vegetative cells differentiate into heterocysts. Quantitative proteomic investigations at both the filament level ...

journal_title：Journal of proteome research

authors： Ow SY,Noirel J,Cardona T,Taton A,Lindblad P,Stensjö K,Wright PC

更新日期：2009-01-01 00:00:00

abstract：:The purpose of this study was to determine the system-wide consequences of deficiencies in two essential micronutrients, vitamins E and C, on the proteome using zebrafish (Danio rerio) as one of the few vertebrate models that similar to humans cannot synthesize vitamin C. We describe a label-free proteomics workflow t...

journal_title：Journal of proteome research

authors： Motorykin I,Traber MG,Tanguay RL,Maier CS

更新日期：2014-03-07 00:00:00

abstract：:The study of glycosylation patterns (glycomics) in biological samples is an emerging field that can provide key insights into cell development and pathology. A current challenge in the field of glycomics is to determine how to quantify changes in glycan expression between different cells, tissues, or biological fluids...

journal_title：Journal of proteome research

authors： Atwood JA 3rd,Cheng L,Alvarez-Manilla G,Warren NL,York WS,Orlando R

更新日期：2008-01-01 00:00:00

abstract：:Protein-protein interactions play a major role in the molecular machinery of life, and various techniques such as AP-MS are dedicated to their identification. However, those techniques return lists of proteins devoid of organizational structure, not detailing which proteins interact with which others. Proposing a hier...

journal_title：Journal of proteome research

authors： Postic G,Marcoux J,Reys V,Andreani J,Vandenbrouck Y,Bousquet MP,Mouton-Barbosa E,Cianférani S,Burlet-Schiltz O,Guerois R,Labesse G,Tufféry P

更新日期：2020-07-02 00:00:00

abstract：:Campylobacter jejuni, an important foodborne microorganism, poses severe and emergent threats to human health as antibiotic resistance becomes increasingly prevalent. The mechanisms of drug resistance are hard to decipher, and little is known at the metabolic level. Here we apply metabolomic profiling to discover meta...

journal_title：Journal of proteome research

authors： Li H,Xia X,Li X,Naren G,Fu Q,Wang Y,Wu C,Ding S,Zhang S,Jiang H,Li J,Shen J

更新日期：2015-02-06 00:00:00