Online microwave D-cleavage LC-ESI-MS/MS of intact proteins: site-specific cleavages at aspartic acid residues and disulfide bonds.

abstract：:Stress in the form of moderate periods of maternal separation of newborn rats has been postulated to cause permanent changes in the central nervous system and diseases in later life. It is also considered that dietary supplementation with long chain polyunsaturated fatty acids (LC-PUFAs) can potentially ameliorate the...

journal_title：Journal of proteome research

authors： Wang Y,Holmes E,Tang H,Lindon JC,Sprenger N,Turini ME,Bergonzelli G,Fay LB,Kochhar S,Nicholson JK

更新日期：2006-07-01 00:00:00

abstract：:We performed systematic evaluation of 38 protocols to concentrate normal human urinary proteins prior to 2D-PAGE analysis. Recovery yield and pattern of resolved protein spots were compared among different methods and intra-/inter-individual variabilities were examined. Precipitation with 90% ethanol provided the grea...

journal_title：Journal of proteome research

authors： Thongboonkerd V,Chutipongtanate S,Kanlaya R

更新日期：2006-01-01 00:00:00

abstract：:The production of virus-like particles (VLPs) has gained importance over the last few years owing to the benefits they provide compared to conventional vaccines. The biopharmaceutical industry is currently searching for safer candidates based on VLPs for new and existing vaccines and implementing new methods of manufa...

journal_title：Journal of proteome research

authors： Lavado-García J,Jorge I,Cervera L,Vázquez J,Gòdia F

更新日期：2020-03-06 00:00:00

abstract：:Investigation of how diatoms cope with the rapid fluctuations in iron bioavailability in marine environments may facilitate a better understanding of the mechanisms underlying their ecological success, in particular their ability to proliferate rapidly during favorable conditions. In this study, using in vivo biochemi...

journal_title：Journal of proteome research

authors： Luo CS,Liang JR,Lin Q,Li C,Bowler C,Anderson DM,Wang P,Wang XW,Gao YH

更新日期：2014-12-05 00:00:00

abstract：:Chronic kidney disease (CKD) has emerged as a major public health problem worldwide. It frequently progresses to end-stage renal disease, which is related to very high cost and mortality. Novel biomarkers can provide insight into the novel mechanism, facilitate early detection, and monitor progression of CKD and its r...

journal_title：Journal of proteome research

authors： Zhang ZH,Chen H,Vaziri ND,Mao JR,Zhang L,Bai X,Zhao YY

更新日期：2016-10-07 00:00:00

abstract：:The zebrafish genome has recently been sequenced and annotated allowing for high-throughput proteomic analysis. Here, we report for the first time a proteomic subset of zebrafish liver, an important organ for metabolizing toxins. Using a newly developed analytical procedure, we have identified 1204 proteins from the c...

journal_title：Journal of proteome research

authors： Wang N,Mackenzie L,De Souza AG,Zhong H,Goss G,Li L

更新日期：2007-01-01 00:00:00

abstract：:Colon cancer is the most common cancer and the third leading cause of cancer mortality in humans. Using mass spectrometry-based metabolomics, the current study revealed the accumulation of four uremic toxins (cresol sulfate, cresol glucuronide, indoxyl sulfate, and phenyl sulfate) in the serum of mice harboring adenom...

journal_title：Journal of proteome research

authors： Li F,Yang XW,Krausz KW,Nichols RG,Xu W,Patterson AD,Gonzalez FJ

更新日期：2015-04-03 00:00:00

abstract：:The development of the nematode Caenorhabditis elegans is a highly dynamic process. Although various studies have assessed global transcriptome changes, information on the dynamics of the proteome during ontogenesis is not available. We metabolically labeled C. elegans by using ¹⁵N ammonium chloride as a precursor in ...

journal_title：Journal of proteome research

authors： Geillinger KE,Kuhlmann K,Eisenacher M,Meyer HE,Daniel H,Spanier B

更新日期：2012-09-07 00:00:00

abstract：:To investigate the role of rpoS in gene expression of Escherichia coli cells grown as biofilms, we compared the proteomes of a rpoS mutant and the wild-type strain. Experiments were performed on planktonic cells (in exponential or stationary growth phase) and biofilms developed on glass wool. Spot-by-spot comparison o...

journal_title：Journal of proteome research

authors： Collet A,Cosette P,Beloin C,Ghigo JM,Rihouey C,Lerouge P,Junter GA,Jouenne T

更新日期：2008-11-01 00:00:00

abstract：:The development of plasma biomarkers has proven to be more challenging than initially anticipated. Many studies have reported lists of candidate proteins rather than validated candidate markers with an assigned performance to a specific clinical objective. Biomarker research necessitates a clear rational framework wit...

journal_title：Journal of proteome research

authors： Surinova S,Schiess R,Hüttenhain R,Cerciello F,Wollscheid B,Aebersold R

更新日期：2011-01-07 00:00:00

abstract：:TGFBR2 serves as an initial regulator of the TGF-β signaling pathway, and loss or reduction of its expression can lead to uncontrolled cell growth. This study was conducted to further explore the mechanism of TGFBR2/SMAD4 on the migration and proliferation of CC cells. Here, TGFBR2 and SMAD4 expressions in CC cells an...

journal_title：Journal of proteome research

authors： Yuan J,Yi K,Yang L

更新日期：2020-08-07 00:00:00

abstract：:The past decade has been marked by the emergence of selective affinity media and sensitive mass spectrometry instrumentation that facilitated large-scale phosphoproteome analyses and expanded the repertoire of protein phosphorylation. Despite these remarkable advances, the precise location of the phosphorylation site ...

journal_title：Journal of proteome research

authors： Courcelles M,Bridon G,Lemieux S,Thibault P

更新日期：2012-07-06 00:00:00

abstract：:O-Linked N-acetyl-β-d-glucosamine (O-GlcNAc) is a dynamic post-translational modification that modifies and regulates over 3000 nuclear, cytoplasmic, and mitochondrial proteins. Upon exposure to stress and injury, cells and tissues increase the O-GlcNAc modification, or O-GlcNAcylation, of numerous proteins promoting ...

journal_title：Journal of proteome research

authors： Lee A,Miller D,Henry R,Paruchuri VD,O'Meally RN,Boronina T,Cole RN,Zachara NE

更新日期：2016-12-02 00:00:00

abstract：:We report the structural analysis of cap-binding proteins using a chemical probe/ultraviolet photodissociation (UVPD) mass spectrometry strategy for evaluating solvent accessibility of proteins. Our methodology utilized a chromogenic probe (NN) to probe the exposed amine residues of wheat eukaryotic translation initia...

journal_title：Journal of proteome research

authors： O'Brien JP,Mayberry LK,Murphy PA,Browning KS,Brodbelt JS

更新日期：2013-12-06 00:00:00

abstract：:Colorectal cancer (CRC) is the third most common cancer worldwide and has poor prognosis. To identify the proteins involved in colorectal carcinogenesis, we employed 2-DE and MALDI-TOF/TOF-based proteomics approach to study the differentially expressed proteins in tumor and adjacent nontumor tissue samples. Samples fr...

journal_title：Journal of proteome research

authors： Ma YL,Peng JY,Zhang P,Huang L,Liu WJ,Shen TY,Chen HQ,Zhou YK,Zhang M,Chu ZX,Qin HL

更新日期：2009-10-01 00:00:00

abstract：:The Baculoviral IAP repeat-containing protein 5 (BIRC5), also known as inhibitor of apoptosis protein survivin, is a member of the chromosomal passenger complex and a key player in mitosis. To investigate the function of BIRC5 in liver regeneration, we analyzed a hepatocyte-specific BIRC5-knockout mouse model using a ...

journal_title：Journal of proteome research

authors： Bracht T,Hagemann S,Loscha M,Megger DA,Padden J,Eisenacher M,Kuhlmann K,Meyer HE,Baba HA,Sitek B

更新日期：2014-06-06 00:00:00

abstract：:An efficient means for the identification of prognostic and predictive biomarkers is essential in today's cancer management. A new approach toward biomarker discovery has therefore been proposed, where pathways instead of individual proteins would be monitored and targeted. Recently, the 'secretome', a biological flui...

journal_title：Journal of proteome research

authors： Lawlor K,Nazarian A,Lacomis L,Tempst P,Villanueva J

更新日期：2009-03-01 00:00:00

abstract：:Energy imbalance is a primary cause of obesity. While the classical approach to attenuate weight gain includes an increase in energy expenditure through exercise, dietary manipulation such as the inclusion of dairy products has also been proven effective. In the present study, we explored the potential mechanisms by w...

journal_title：Journal of proteome research

authors： Mayengbam S,Mickiewicz B,Trottier SK,Mu C,Wright DC,Reimer RA,Vogel HJ,Shearer J

更新日期：2019-11-01 00:00:00

abstract：:The domain graph of domains and domain combinations of Arabidopsis thaliana is established based on pfam 14.0 database and analyzed via comparison with 10 eukaryotic, 30 bacterial, and 16 archaeal proteomes. The comparative analysis of the domain graphs provides a useful platform for revealing global insights on the e...

journal_title：Journal of proteome research

authors： Liu S,Zhang C,Zhou Y

更新日期：2005-03-01 00:00:00

abstract：:Major depressive disorder (MDD) is a debilitating mental disease with a pronounced impact on the quality of life of many people; however, it is still difficult to diagnose MDD accurately. In this study, a nontargeted metabolomics approach based on ultra-high-performance liquid chromatography equipped with quadrupole t...

journal_title：Journal of proteome research

authors： Liu X,Zheng P,Zhao X,Zhang Y,Hu C,Li J,Zhao J,Zhou J,Xie P,Xu G

更新日期：2015-05-01 00:00:00

abstract：:Papillary thyroid carcinoma (PTC) is the most common type of thyroid cancer among women worldwide. It is confirmed mainly by fine-needle aspiration biopsy (FNAB), an invasive diagnostic method. The key proteins responsible for thyroid hormone biosynthesis are glycosylated. Hence, changes in site-specific glycosylation...

journal_title：Journal of proteome research

authors： Zhang Y,Zhao W,Zhao Y,Mao Y,Su T,Zhong Y,Wang S,Zhai R,Cheng J,Fang X,Zhu J,Yang H

更新日期：2020-07-02 00:00:00

abstract：:Acute stress may trigger systemic biochemical and physiological changes in living organisms, leading to a rapid loss of homeostasis, which can be gradually reinstated by self-regulatory mechanisms and/or drug intervention strategy. However, such a sophisticated metabolic regulatory process has so far been poorly under...

journal_title：Journal of proteome research

authors： Wang X,Su M,Qiu Y,Ni Y,Zhao T,Zhou M,Zhao A,Yang S,Zhao L,Jia W

更新日期：2007-09-01 00:00:00

abstract：:Acute kidney injury (AKI) is a frequent complication after cardiopulmonary bypass, but early detection of postoperative AKI remains challenging. Protein biomarkers predict AKI excellently in homogeneous cohorts but are less reliable in patients suffering from various comorbidities. We employed nuclear magnetic resonan...

journal_title：Journal of proteome research

authors： Zacharias HU,Hochrein J,Vogl FC,Schley G,Mayer F,Jeleazcov C,Eckardt KU,Willam C,Oefner PJ,Gronwald W

更新日期：2015-07-02 00:00:00

abstract：:Rhizobia, the nitrogen-fixing bacterial symbionts of legumes, represent an agricultural application of primary relevance and a model of plant-microbe molecular dialogues. We recently described rhizobium proteome alterations induced by plant flavonoids using iTRAQ. Herein, we further extend that experimentation, provin...

journal_title：Journal of proteome research

authors： Arrigoni G,Tolin S,Moscatiello R,Masi A,Navazio L,Squartini A

更新日期：2013-11-01 00:00:00

abstract：:A simple and site-specific nonenzymatic method based on pyrolysis has been developed to cleave peptides and proteins. Pyrolytic cleavage was found to be specific and rapid as it induced a cleavage at the C-terminal side of aspartic acid in the temperature range of 220-250 degrees C in 10 s. Electrospray ionization (ES...

journal_title：Journal of proteome research

authors： Zhang S,Basile F

更新日期：2007-05-01 00:00:00

abstract：:Acinetobacter baumannii causes severe infections in compromised patients. We combined SDS-PAGE, two-dimensional gel electrophoresis and mass spectrometry (LC-MS/MS and MALDI-TOF) to separate and characterize the proteins of the cell envelope of this bacterium. In total, 135 proteins (inner and outer membrane proteins)...

journal_title：Journal of proteome research

authors： Siroy A,Cosette P,Seyer D,Lemaître-Guillier C,Vallenet D,Van Dorsselaer A,Boyer-Mariotte S,Jouenne T,Dé E

更新日期：2006-12-01 00:00:00

abstract：:Growing appreciation for astrocytes as active participants in nervous system development, neurovascular metabolic coupling, and neurological disease progression has stimulated recent investigation into specific astrocyte-secreted proteins that may mediate these functions. The current work utilized SILAC-generated isot...

journal_title：Journal of proteome research

authors： Greco TM,Seeholzer SH,Mak A,Spruce L,Ischiropoulos H

更新日期：2010-05-07 00:00:00

abstract：:Protein-protein control recognition remains a huge challenge, and its development depends on understanding the chemical and biological mechanisms by which these interactions occur. Here we describe a protein-protein control recognition technique based on the dominant electrostatic interactions occurring between the pr...

journal_title：Journal of proteome research

authors： Han S,Yin S,Yi H,Mouhat S,Qiu S,Cao Z,Sabatier JM,Wu Y,Li W

更新日期：2010-06-04 00:00:00

abstract：:In this Letter, we reanalyze published mass spectrometry data sets of clinical samples with a focus on determining the coinfection status of individuals infected with SARS-CoV-2 coronavirus. We demonstrate the use of ComPIL 2.0 software along with a metaproteomics workflow within the Galaxy platform to detect cohabita...

journal_title：Journal of proteome research

authors： Thuy-Boun PS,Mehta S,Gruening B,McGowan T,Nguyen A,Rajczewski AT,Johnson JE,Griffin TJ,Wolan DW,Jagtap PD

更新日期：2021-01-04 00:00:00

abstract：:The current report describes the use of CapLC-ESI-Q/TOF-MS for investigating the proteome profiles of hypertonic saline-induced sputum samples from 56 smokers. The severity of their lung disease ranged from normal (healthy smokers) to chronic bronchitis, chronic obstructive pulmonary disease (COPD), and COPD with emph...

journal_title：Journal of proteome research

authors： Casado B,Iadarola P,Pannell LK,Luisetti M,Corsico A,Ansaldo E,Ferrarotti I,Boschetto P,Baraniuk JN

更新日期：2007-12-01 00:00:00