Abstract：
:Liver tissue engineering requires a perfect extracellular matrix (ECM) for primary hepatocytes culture to maintain high level of liver-specific functions and desirable mechanical stability. The aim of this study was to develop a novel natural nanofibrous scaffold with surface-galactose ligands to enhance the bioactivity and mechanical stability of primary hepatocytes in culture. The nanofibrous scaffold was fabricated by electrospinning a natural material, galactosylated chitosan (GC), into nanofibers with an average diameter of approximately 160 nm. The GC nanofibrous scaffolds displayed slow degradation and suitable mechanical properties as an ECM for hepatocytes according to the evaluation of disintegration and Young's modulus testing. The results of morphology characterization, double-staining fluorescence assay and function detection showed that hepatocytes cultured on GC nanofibrous scaffold formed stably immobilized 3D flat aggregates and exhibited superior cell bioactivity with higher levels of liver-specific function maintenance in terms of albumin secretion, urea synthesis and cytochrome P-450 enzyme than 3D spheroid aggregates formed on GC films. These spheroid aggregates could be detached easily during culture period from the flat GC films. We suggest such GC-based nanofibrous scaffolds could be useful for various applications such as bioartificial liver-assist devices and tissue engineering for liver regeneration as primary hepatocytes culture substrates.
journal_name
Biomaterialsjournal_title
Biomaterialsauthors
Feng ZQ,Chu X,Huang NP,Wang T,Wang Y,Shi X,Ding Y,Gu ZZdoi
10.1016/j.biomaterials.2009.01.053subject
Has Abstractpub_date
2009-05-01 00:00:00pages
2753-63issue
14eissn
0142-9612issn
1878-5905pii
S0142-9612(09)00112-4journal_volume
30pub_type
杂志文章相关文献
BIOMATERIALS文献大全abstract：:Despite recent advances in cardiovascular medicine, ischemic diseases remain a major cause of morbidity and mortality. Although stem cell-based therapies for the treatment of ischemic diseases show great promise, limited availability of biologically functional stem cells mired the application of stem cell-based therap...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2014.06.037
更新日期：2014-10-01 00:00:00
abstract：:Knee arthroplasty has reached a high degree of reliability. Many of the remaining long-term loosening cases are due to the polyethylene wear debris. The introduction of the alumina ceramic to polyethylene bearing couple had been a promising means for its reduction. The discussion of its applications in total knee repl...
journal_title：Biomaterials
pub_type： 杂志文章,评审
doi：10.1016/s0142-9612(01)00262-9
更新日期：2002-04-01 00:00:00
abstract：:Atherosclerotic plaques are cholesterol-induced inflammatory niches accumulating in the vascular sub-endothelial space. Cellular and extracellular composition of human plaques is maneuvered by local inflammation that leads to alterations in the original vascular microenvironment and to the recruitment of an invading f...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2017.09.034
更新日期：2018-01-01 00:00:00
abstract：:Very elastic poly(L-lactide-co-epsilon-caprolactone) (PLCL) (50:50) copolymer blended with gelatin was electrospun into microfibers from a hexafluoroisopropanol solution. PLCL fiber sheet exhibited the unique soft and flexible behavior while gelatin fiber was hard and brittle. As the gelatin content of PLCL/gelatin fi...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2007.12.029
更新日期：2008-04-01 00:00:00
abstract：:Particulate wear debris of artificial joints, metallic and plastic, and solutions of metals in body fluids are known to have adverse effects. Encapsulation of the articulating parts would prevent wear debris from entering the surrounding tissue and check tissue ingrowth into the joint. The capsule could contain an art...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/0142-9612(80)90056-3
更新日期：1980-01-01 00:00:00
abstract：:Demineralised bone matrix (DBM) is a form of allogeneic tissue graft widely used in oral and maxillofacial procedures. There is a long history of controversy relating to the suitability of ethylene oxide gas (EtOx) as a terminal sterilisation agent for this graft, relating to its effects on the clinical performance of...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(00)00255-6
更新日期：2001-05-01 00:00:00
abstract：:Safety and high efficacy of vectors are essential requirements for gene therapy. To address these challenges, poly(carboxy betaine methacrylate ethyl ester)-poly(carboxy betaine methacrylate) (PCBMAEE-PCBMA) diblock copolymers were synthesized to form core-shell vector for gene delivery. The hydrophobic PCBMAEE segmen...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2014.05.066
更新日期：2014-09-01 00:00:00
abstract：:3D printing is known as a cost-effective technique that shows huge potential in fabrication of graft substitutes for bone tissue regeneration. However, the tradeoff between 3D printability, mechanical strength and bioactivity of the printed materials (i.e., inks) remains a challenge. In this work, we present a novel p...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2020.120378
更新日期：2020-12-01 00:00:00
abstract：:Matrix systems used to study complex three-dimensional (3D) cellular processes like mammary epithelial tissue morphogenesis and tumorigenesis ex vivo often require ill-defined biological components, which lead to poor reproducibility and a lack of control over physical parameters. In this study, a well-defined, tunabl...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2016.10.007
更新日期：2017-01-01 00:00:00
abstract：:Silicone elastomers have been used as implants in orthopaedics for replacements of small joints such as the metacarpophalangeal joint. A series of experiments was conducted an axially tensioned silicone elastomer tendon spacer samples to determine whether there is a relationship between stress in the material and imag...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(97)00155-5
更新日期：1998-01-01 00:00:00
abstract：:The aim of this study was to evaluate biologic behavior of a composite of bioactive glass (BAG) (S53P4) and copolymer of poly(epsilon-caprolactone-co-DL-lactide) in experimental bone defects in rabbits. Twenty New Zealand white rabbits were used for the study. Bone defects (4 x 6mm) were prepared in the medial surface...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(02)00546-x
更新日期：2003-05-01 00:00:00
abstract：:To enable the synthesis of hydrogels containing microporosity, a family of pore generating additives (porogens) were synthesized from poly(ethylene glycols) (PEGs) with different molecular weights using naphthyl acetic acid. The products formed were characterized by UV-vis, differential scanning calorimetry and soluti...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/0142-9612(93)90206-h
更新日期：1993-11-01 00:00:00
abstract：:Photodynamic therapy (PDT) is a new therapeutic approach for the palliative treatment of malignant bile duct obstruction. In this study, we designed photosensitizer-embedded self-expanding nonvascular metal stent (PDT-stent) which allows repeatable photodynamic treatment of cholangiocarcinoma without systemic injectio...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2014.07.001
更新日期：2014-10-01 00:00:00
abstract：:Nanotechnology-based drug delivery systems have been intensively investigated, while only a few of them can be used for clinic application. Hematoporphyrin (HP), a major molecule in erythrocyte, has been widely used in photodynamic therapy (PDT). In the present study, polyethylene glycol (PEG) modified hematoporphyrin...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2013.12.004
更新日期：2014-03-01 00:00:00
abstract：:Poly(γ-4-((2-(piperidin-1-yl)ethyl)aminomethyl)benzyl-l-glutamate) (PPABLG), a cationic helical polypeptide, has been recently developed by us as an effective non-viral gene delivery vector. In attempts to elucidate the effect of molecular architecture on the gene delivery efficiencies and thereby identify a potential...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2012.11.064
更新日期：2013-03-01 00:00:00
abstract：:Programmable hydrogels are defined as hydrogels that are able to change their properties and functions periodically, reversibly and/or sequentially on demand. They are different from those responsive hydrogels whose changes are passive or cannot be stopped or reversed once started and vice versa. The purpose of this r...
journal_title：Biomaterials
pub_type： 杂志文章,评审
doi：10.1016/j.biomaterials.2018.03.008
更新日期：2018-09-01 00:00:00
abstract：:Polypyrrole (PPy) is a conducting polymer and is obtained electrochemically on an electrode such as indium-tin oxide (ITO). In this study, in order to develop a novel cell-culture system which makes it possible to communicate with cultured mammalian cells, bovine adrenal chromaffin cells were cultured on PPy-coated IT...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/0142-9612(96)00015-4
更新日期：1996-10-01 00:00:00
abstract：:Tissue engineering requires the use of polymeric scaffolds that mimic many roles of extracellular matrices (ECM) in the body. Controlling cell-scaffold interactions is one of the most critical parameters for regulating cell phenotype in tissue engineering, and a peptide with the sequence of RGD has been widely exploit...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2010.03.063
更新日期：2010-07-01 00:00:00
abstract：:In the present work, we designed and synthesized a novel mono-guanidine heterolipid (MGH) and confirmed its structure by NMR and ESI-MS. The MGH was used as cationic lipid in developing etoposide loaded cationic self-microemulsifying drug delivery system (ECS) intended to be delivered by intratumoral route. The ECS ex...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2015.03.040
更新日期：2015-07-01 00:00:00
abstract：:The effect of surface characteristics on the interaction of sterically stabilized polystyrene particles with mouse peritoneal macrophages was studied using a range of poloxamers and poloxamine as coating agents. The coated particles were characterized in terms of thickness of coating layer, surface charge and critical...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/0142-9612(87)90099-8
更新日期：1987-03-01 00:00:00
abstract：:A glucose biosensor was fabricated with loading of glucose oxidase (GOx) into a new organic-inorganic hybrid nanocomposite. The preparation involves formation of silica network into a Nafion (perfluorosulfonate ionomer) and subsequent loading of polyaniline grafted multiwalled carbon nanotubes (MWNT-g-PANI) onto Nafio...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2009.07.047
更新日期：2009-10-01 00:00:00
abstract：:The non-collagenous proteins of mature cortical rabbit bone and 3 wk old bone specimens formed in titanium implant bone harvest chambers, were separated by means of fine pressure liquid chromatography. It was found that a sufficient amount of bone could be obtained from five bone harvest chambers in five rabbits durin...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/0142-9612(90)90158-m
更新日期：1990-04-01 00:00:00
abstract：:The adsorption of bovine serum albumin (BSA) on to titanium powder has been studied as a function of protein concentration and pH, and in the presence of calcium and phosphate ions. Isotherm data have shown that the adsorption process does not follow the Langmuir model (inflection points). The maximum adsorption (Adma...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/0142-9612(96)83280-7
更新日期：1996-05-01 00:00:00
abstract：:Recent studies have revealed that visible-light (VL)-irradiated camphorquinone (CQ), in the presence of a tertiary amine (e.g., N,N-dimethyl-p-toluidine, DMT), generates initiating radicals that may indiscriminately react with molecular oxygen forming reactive oxygen species (ROS). In this study, the ability of the an...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2005.04.001
更新日期：2005-11-01 00:00:00
abstract：:Valvular interstitial cells (VICs) actively maintain and repair heart valve tissue; however, persistent activation of VICs to a myofibroblast phenotype can lead to aortic stenosis. To better understand and quantify how microenvironmental cues influence VIC phenotype and myofibroblast activation, we compared expression...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2015.09.035
更新日期：2016-01-01 00:00:00
abstract：:Nickel-titanium (NiTi, nitinol) shape memory alloy was nitrided using an original powder immersion reaction assisted coating (PIRAC) method in order to modify its surface properties. PIRAC nitriding method is based on annealing the samples in the atmosphere of highly reactive nitrogen supplied by decomposition of unst...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(00)00368-9
更新日期：2001-07-01 00:00:00
abstract：:Reactive diluents such as triethyleneglycol-dimethacrylate (TEGDMA) have been widely used with bisphenol-A-glycidyl-dimethacrylate (Bis-GMA) to achieve restorative resins with appropriate viscosity and higher conversion. However, additional water sorption and polymerization shrinkage were also introduced. The aim of t...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2004.04.041
更新日期：2005-04-01 00:00:00
abstract：:Compared to soluble cytokines, surface-tethered ligands can deliver biological signalling with precise control of spatial positioning and concentration. A strategy that immobilises ligand molecules on a surface in a uniform orientation using non-cleavable linkages under physiological conditions would enhance the speci...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2014.10.029
更新日期：2015-01-01 00:00:00
abstract：:A photografting technique to produce functional groups of silanol able to induce apatite nucleation was attempted on polyethylene substrate for biomimetic formation of bone-mineral-like apatite layer on its surface. The polyethylene surface was subjected to vapor-phase photografting of vinyltrimethoxysilane and subseq...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(00)00437-3
更新日期：2001-09-01 00:00:00
abstract：:Local signals from tissue-specific extracellular matrix (ECM) microenvironments, including matrix adhesive ligand, mechanical elasticity and micro-scale geometry, are known to instruct a variety of stem cell differentiation processes. Likewise, these signals converge to provide multifaceted, mechanochemical cues for h...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2015.04.036
更新日期：2015-08-01 00:00:00
