当前位置： SCI文献检索 > BIOMATERIALS期刊下所有文献 > Microarray analyses to quantify advantages of 2D and 3D hydrogel culture systems in maintaining the native valvular interstitial cell phenotype.

Microarray analyses to quantify advantages of 2D and 3D hydrogel culture systems in maintaining the native valvular interstitial cell phenotype.

Abstract：

:Valvular interstitial cells (VICs) actively maintain and repair heart valve tissue; however, persistent activation of VICs to a myofibroblast phenotype can lead to aortic stenosis. To better understand and quantify how microenvironmental cues influence VIC phenotype and myofibroblast activation, we compared expression profiles of VICs cultured on poly(ethylene glycol) (PEG) gels to those cultured on tissue culture polystyrene (TCPS), as well as fresh isolates. In general, VICs cultured in hydrogel matrices had lower levels of activation (<10%), similar to levels seen in healthy valve tissue, while VICs cultured on TCPS were ∼75% activated myofibroblasts. VICs cultured on TCPS also exhibited a higher magnitude of perturbations in gene expression than soft hydrogel cultures when compared to the native phenotype. Using peptide-modified PEG gels, VICs were seeded on (2D), as well as encapsulated in (3D), matrices of the same composition and modulus. Despite similar levels of activation, VICs cultured in 2D had distinct variations in transcriptional profiles compared to those in 3D hydrogels. Genes related to cell structure and motility were particularly affected by the dimensionality of the culture platform, with higher expression levels in 2D than in 3D. These results indicate that dimensionality may play a significant role in dictating cell phenotype (e.g., through differences in polarity, diffusion of soluble signals), and emphasize the importance of using multiple metrics when characterizing cell phenotype.

journal_name

Biomaterials

journal_title

Biomaterials

authors

Mabry KM,Payne SZ,Anseth KS

doi

10.1016/j.biomaterials.2015.09.035

subject

Has Abstract

pub_date

2016-01-01 00:00:00

pages

31-41

eissn

0142-9612

issn

1878-5905

pii

S0142-9612(15)00786-3

journal_volume

74

pub_type

杂志文章

相关文献

BIOMATERIALS文献大全
  • Bioreducible polymer-transfected skeletal myoblasts for VEGF delivery to acutely ischemic myocardium.

    abstract：:Implantation of skeletal myoblasts to the heart has been investigated as a means to regenerate and protect the myocardium from damage after myocardial infarction. While several animal studies utilizing skeletal myoblasts have reported positive findings, results from clinical studies have been mixed. In this study we u...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/j.biomaterials.2010.09.061

    authors： McGinn AN,Nam HY,Ou M,Hu N,Straub CM,Yockman JW,Bull DA,Kim SW

    更新日期：2011-01-01 00:00:00

  • Topographical cues control the morphology and dynamics of migrating cortical interneurons.

    abstract：:In mammalian embryos, cortical interneurons travel long distances among complex three-dimensional tissues before integrating into cortical circuits. Several molecular guiding cues involved in this migration process have been identified, but the influence of physical parameters remains poorly understood. In the present...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/j.biomaterials.2019.05.005

    authors： Leclech C,Renner M,Villard C,Métin C

    更新日期：2019-09-01 00:00:00

  • Dynamic tissue engineering scaffolds with stimuli-responsive macroporosity formation.

    abstract：:Macropores in tissue engineering scaffolds provide space for vascularization, cell-proliferation and cellular interactions, and is crucial for successful tissue regeneration. Modulating the size and density of macropores may promote desirable cellular processes at different stages of tissue development. Most current t...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/j.biomaterials.2013.02.051

    authors： Han LH,Lai JH,Yu S,Yang F

    更新日期：2013-06-01 00:00:00

  • Biostability and biological performance of a PDMS-based polyurethane for controlled drug release.

    abstract：:Polymers have been used to deliver therapeutic agents in a range of medical devices with drug eluting stents being the most widespread current application. Although polymers enable controlled release of a therapeutic agent, the polymeric surface has been reported to provide suboptimal biocompatibility and haemocompati...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/j.biomaterials.2008.04.007

    authors： Simmons A,Padsalgikar AD,Ferris LM,Poole-Warren LA

    更新日期：2008-07-01 00:00:00

  • ROS-augmented and tumor-microenvironment responsive biodegradable nanoplatform for enhancing chemo-sonodynamic therapy.

    abstract：:Nanocarrier for augmenting the efficacy of reactive oxygen species (ROS) by tumor microenvironment (TME) has become an emerging strategy for cancer treatment. Herein, a smart biodegradable drug delivery nanoplatform with mitochondrial-targeted ability, pH-responsive drug release and enzyme-like catalytic function is d...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/j.biomaterials.2020.119761

    authors： An J,Hu YG,Cheng K,Li C,Hou XL,Wang GL,Zhang XS,Liu B,Zhao YD,Zhang MZ

    更新日期：2020-03-01 00:00:00

  • Patient-derived scaffolds uncover breast cancer promoting properties of the microenvironment.

    abstract：:Tumor cells interact with the microenvironment that specifically supports and promotes tumor development. Key components in the tumor environment have been linked to various aggressive cancer features and can further influence the presence of subpopulations of cancer cells with specific functions, including cancer ste...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/j.biomaterials.2019.119705

    authors： Landberg G,Fitzpatrick P,Isakson P,Jonasson E,Karlsson J,Larsson E,Svanström A,Rafnsdottir S,Persson E,Gustafsson A,Andersson D,Rosendahl J,Petronis S,Ranji P,Gregersson P,Magnusson Y,Håkansson J,Ståhlberg A

    更新日期：2020-03-01 00:00:00

  • Problems of encapsulation of total joint replacements.

    abstract：:Particulate wear debris of artificial joints, metallic and plastic, and solutions of metals in body fluids are known to have adverse effects. Encapsulation of the articulating parts would prevent wear debris from entering the surrounding tissue and check tissue ingrowth into the joint. The capsule could contain an art...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/0142-9612(80)90056-3

    authors： Derbyshire B,Skorecki J,Wheble VH

    更新日期：1980-01-01 00:00:00

  • Effect of dual ion implantation of calcium and phosphorus on the properties of titanium.

    abstract：:This study is concerned with the effect of dual implantation of calcium and phosphorus upon the structure, corrosion resistance and biocompatibility of titanium. The ions were implanted in sequence, first Ca and then P, both at a dose of 10(17) ions/cm2 at a beam energy of 25 keV. Transmission electron microscopy was ...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/j.biomaterials.2004.08.015

    authors： Krupa D,Baszkiewicz J,Kozubowski JA,Barcz A,Sobczak JW,Biliński A,Lewandowska-Szumieł M,Rajchel B

    更新日期：2005-06-01 00:00:00

  • Assembly of upconversion nanophotosensitizer in vivo to achieve scatheless real-time imaging and selective photodynamic therapy.

    abstract：:A perfect "off" to "on" switch of the therapeutic function is very important to minimize the phototoxicity of nanoplatforms assisted imaging-guided photodynamic therapy (PDT) of cancer. Current approaches rely on preloaded photosensitizers, where the off/on state of PDT is regulated by the sensitizing light of photose...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/j.biomaterials.2019.02.015

    authors： Feng Y,Wu Y,Zuo J,Tu L,Que I,Chang Y,Cruz LJ,Chan A,Zhang H

    更新日期：2019-05-01 00:00:00

  • Viscoelastic behaviour of acrylic bone cements.

    abstract：:Local contact stresses at the bone-cement interface are thought to play an important role in the initiation of component loosening. A reduced-modulus bone cement can lower these local contact stresses. The viscoelastic properties of such a cement raised the question of long term subsidence of the implant system. In th...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/s0142-9612(97)00115-4

    authors： Yetkinler DN,Litsky AS

    更新日期：1998-09-01 00:00:00

  • The influence of substrate stiffness on the behavior and functions of Schwann cells in culture.

    abstract：:Solid tissues in the body possess a range of stiffness and provide cells with an instructive microenvironment. Scaffolds in tissue engineering should be rationally designed to become an adhesion substrate friendly to cells. Schwann cells are the principal glial cell in the peripheral nervous system and used as support...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/j.biomaterials.2012.06.006

    authors： Gu Y,Ji Y,Zhao Y,Liu Y,Ding F,Gu X,Yang Y

    更新日期：2012-10-01 00:00:00

  • Electrospun chitosan-based nanofibers and their cellular compatibility.

    abstract：:Chitosan-based nanofibers with an average fiber diameter controllable from a few microns down to approximately 40 nm and a narrow size distribution were fabricated by electrospinning solutions containing chitosan, polyethylene oxide (PEO), and Triton X-100. Rheological study showed a strong dependence of spinnability ...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/j.biomaterials.2005.03.027

    authors： Bhattarai N,Edmondson D,Veiseh O,Matsen FA,Zhang M

    更新日期：2005-11-01 00:00:00

  • Osteoblast behavior on polytetrafluoroethylene modified by long pulse, high frequency oxygen plasma immersion ion implantation.

    abstract：:Polytetrafluoroethylene (PTFE) is a commonly used medical polymer due to its biological stability and other attractive properties such as high hardness and wear resistance. However, the low surface energy and lack of functional groups to interact with the cellular environment have severely limited its applications in ...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/j.biomaterials.2009.09.066

    authors： Wang H,Kwok DT,Wang W,Wu Z,Tong L,Zhang Y,Chu PK

    更新日期：2010-01-01 00:00:00

  • Corrosion behavior of copper/LDPE nanocomposites in simulated uterine solution.

    abstract：:We prepared copper/low-density polyethylene (LDPE) nanocomposites with various mass fractions of copper nanoparticles and investigated the Cu(2+) release rate of them for 220 days in a simulated uterine solution .The influences of copper nanoparticle mass fraction on Cu(2+) release and volume resistivity confirm that ...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/j.biomaterials.2004.08.003

    authors： Cai S,Xia X,Xie C

    更新日期：2005-05-01 00:00:00

  • Surface-modified GVs as nanosized contrast agents for molecular ultrasound imaging of tumor.

    abstract：:Nanobubbles, as a kind of new ultrasound contrast agent (UCAs), have shown promise to penetrate tumor vasculature to allow for targeted imaging. However, their inherent physical instability is an ongoing concern that could weaken their imaging ability with ultrasound. Gas vesicles (GVs), which are genetically encoded,...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/j.biomaterials.2020.119803

    authors： Wang G,Song L,Hou X,Kala S,Wong KF,Tang L,Dai Y,Sun L

    更新日期：2020-04-01 00:00:00

  • Treatment of osteomyelitis with teicoplanin-encapsulated biodegradable thermosensitive hydrogel nanoparticles.

    abstract：:Osteomyelitis characterized by an inflammatory response often leads to bone loss and the spread of bacterial infection to surrounding soft tissues. To overcome the side effects induced by the systemic antibiotic treatment for osteomyelitis, recent investigations have explored the use of antibiotic-loaded undegradable ...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/j.biomaterials.2010.03.027

    authors： Peng KT,Chen CF,Chu IM,Li YM,Hsu WH,Hsu RW,Chang PJ

    更新日期：2010-07-01 00:00:00

  • Vitamin E TPGS as a molecular biomaterial for drug delivery.

    abstract：:D-α-tocopheryl polyethylene glycol succinate (Vitamin E TPGS, or simply TPGS) is a water-soluble derivative of natural Vitamin E, which is formed by esterification of Vitamin E succinate with polyethylene glycol (PEG). As such, it has advantages of PEG and Vitamin E in application of various nanocarriers for drug deli...

    journal_title：Biomaterials

    pub_type： 杂志文章,评审

    doi：10.1016/j.biomaterials.2012.03.046

    authors： Zhang Z,Tan S,Feng SS

    更新日期：2012-06-01 00:00:00

  • Differential characterization of hepatic tumors in MR imaging by burst-released Mn2+-ions from hollow manganese-silicate nanoparticles in the liver.

    abstract：:Gd3+-based contrast agents monopolize in the clinical MR imaging-based diagnosis of hepatic tumors, however, the inherent toxicity by the released Gd3+-ions triggered an urgent demand for safer alternatives. Here, we demonstrate hollow manganese silicate nanoparticles (HMS), which exert burst-release of Mn2+-ions by s...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/j.biomaterials.2019.119600

    authors： Kim JG,Jang MS,Kumari N,Choi JK,Im GH,Kwon T,Lee JH,Lee WJ,Lee IS

    更新日期：2020-02-01 00:00:00

  • Targeted reversal and phosphorescence lifetime imaging of cancer cell metabolism via a theranostic rhenium(I)-DCA conjugate.

    abstract：:Cancer cell metabolism is quite different from normal cells. Targeting cancer metabolism and untuning the tumor metabolic machine has emerged as a promising strategy for cancer therapy. We have developed a multi-functional Re-dca conjugate (Re-dca 2) by conjugating the metabolic modulator dichloroacetate (DCA) to mito...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/j.biomaterials.2018.05.040

    authors： Yang J,Cao Q,Zhang H,Hao L,Zhou D,Gan Z,Li Z,Tong YX,Ji LN,Mao ZW

    更新日期：2018-09-01 00:00:00

  • Structural alterations of p(HEMA)--collagen implants.

    abstract：:Samples of linear (additionally crosslinked) p(HEMA) with different amounts of fibrillar collagen were implanted into the popliteal region of rats. After 3 month, the implanted materials were harvested and examined by SEM. The implants underwent marked structural or morphological changes. While the fibrillar collagen ...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/0142-9612(91)90142-w

    authors： Stol M,Cífková I,Tyrácková V,Adam M

    更新日期：1991-07-01 00:00:00

  • A synthetic stroma-free germinal center niche for efficient generation of humoral immunity ex vivo.

    abstract：:B cells play a major role in the adaptive immune response by producing antigen-specific antibodies against pathogens and imparting immunological memory. Following infection or vaccination, antibody-secreting B cells and memory B cells are generated in specialized regions of lymph nodes and spleens, called germinal cen...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/j.biomaterials.2018.02.039

    authors： Roh KH,Song HW,Pradhan P,Bai K,Bohannon CD,Dale G,Leleux J,Jacob J,Roy K

    更新日期：2018-05-01 00:00:00

  • The promotion of bone healing by progranulin, a downstream molecule of BMP-2, through interacting with TNF/TNFR signaling.

    abstract：:Endochondral ossification plays a key role in the bone healing process, which requires normal cartilage callus formation. Progranulin (PGRN) growth factor is known to enhance chondrocyte differentiation and endochondral ossification during development, yet whether PGRN also plays a role in bone regeneration remains un...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/j.biomaterials.2013.05.030

    authors： Zhao YP,Tian QY,Frenkel S,Liu CJ

    更新日期：2013-09-01 00:00:00

  • A multifunctional anti-inflammatory drug that can specifically target activated macrophages, massively deplete intracellular H2O2, and produce large amounts CO for a highly efficient treatment of osteoarthritis.

    abstract：:Specifically inhibiting the proliferation of activated macrophages and clearing the high levels of reactive oxygen species (ROS) secreted by macrophages is crucial for osteoarthritis (OA) treatment. Moreover, if the clearance of these high levels of ROS can be simultaneously used to induce oxidation-responsive release...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/j.biomaterials.2020.120155

    authors： Yang G,Fan M,Zhu J,Ling C,Wu L,Zhang X,Zhang M,Li J,Yao Q,Gu Z,Cai X

    更新日期：2020-10-01 00:00:00

  • Secretory function of adrenal chromaffin cells cultured on polypyrrole films.

    abstract：:Polypyrrole (PPy) is a conducting polymer and is obtained electrochemically on an electrode such as indium-tin oxide (ITO). In this study, in order to develop a novel cell-culture system which makes it possible to communicate with cultured mammalian cells, bovine adrenal chromaffin cells were cultured on PPy-coated IT...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/0142-9612(96)00015-4

    authors： Aoki T,Tanino M,Sanui K,Ogata N,Kumakura K

    更新日期：1996-10-01 00:00:00

  • Synthetic probes for in vitro purification and in vivo tracking of hepatocytes derived from human pluripotent stem cells.

    abstract：:Hepatocytes derived from human pluripotent stem cells (hPSCs) are promising candidates for cell therapy and drug discovery. However, it remains challenging to efficiently purify hepatocytes from undesired cell types after differentiation and to accurately monitor grafted cells after transplantation. Indocyanine Green ...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/j.biomaterials.2019.119431

    authors： Park JY,Han J,Jung HS,Lee G,Kim HJ,Cho GS,Park HJ,Han C,Kim JS,Kim JH

    更新日期：2019-11-01 00:00:00

  • Regulation of vascular smooth muscle cell autophagy by DNA nanotube-conjugated mTOR siRNA.

    abstract：:The efficient delivery of short interfering RNA (siRNA) is an enormous challenge in the field of gene therapy. Herein, we report a delivery nanosystem based on programmed DNA self-assembly mammalian target of rapamycin (mTOR) siRNA-loaded DNA nanotubes (DNA-NTs). We demonstrate that these siRNA-DNA-NTs can be effectiv...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/j.biomaterials.2015.07.015

    authors： You Z,Qian H,Wang C,He B,Yan J,Mao C,Wang G

    更新日期：2015-10-01 00:00:00

  • Growth of hollow cell spheroids in microbead templated chambers.

    abstract：:Cells form hollow, spheroidal structures during the development of many tissues, including the ocular lens, inner ear, and many glands. Therefore, techniques for in vitro formation of hollow spheroids are valued for studying developmental and disease processes. Current in vitro methods require cells to self-organize i...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/j.biomaterials.2017.07.031

    authors： Wang E,Wang D,Geng A,Seo R,Gong X

    更新日期：2017-10-01 00:00:00

  • Study of a (trimethylenecarbonate-co-epsilon-caprolactone) polymer part 1: preparation of a new nerve guide through controlled random copolymerization using rare earth catalysts.

    abstract：:Random copolymers of trimethylene carbonate and epsilon-caprolactone have been synthesized through ring-opening polymerization using rare earth alkoxides as initiator. The structure of the copolymers has been characterized by 1H and 13C NMR. Their thermal behaviour, their permeability to liquid and their mechanical pr...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/s0142-9612(01)00029-1

    authors： Schappacher M,Fabre T,Mingotaud AF,Soum A

    更新日期：2001-11-01 00:00:00

  • Cell adhesion on phase-separated surface of block copolymer composed of poly(2-methacryloyloxyethyl phosphorylcholine) and poly(dimethylsiloxane).

    abstract：:We investigated the morphological effect of phase-separated block copolymer surfaces composed of poly(2-methacryloyloxyethyl phosphorylcholine (MPC)) (PMPC) and poly(dimethylsiloxane) (PDMS) on protein adsorption and cell adhesion behavior. We observed three different types of phase-separated surface morphologies by T...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/j.biomaterials.2009.06.031

    authors： Seo JH,Matsuno R,Takai M,Ishihara K

    更新日期：2009-10-01 00:00:00

  • Integrin-assisted drug delivery of nano-scaled polymer therapeutics bearing paclitaxel.

    abstract：:Angiogenesis plays a prominent role in cancer progression. Anti-angiogenic therapy therefore, either alone or in combination with conventional cytotoxic therapy, offers a promising therapeutic approach. Paclitaxel (PTX) is a widely-used potent cytotoxic drug that also exhibits anti-angiogenic effects at low doses. How...

    journal_title：Biomaterials

    pub_type： 杂志文章

    doi：10.1016/j.biomaterials.2011.01.073

    authors： Eldar-Boock A,Miller K,Sanchis J,Lupu R,Vicent MJ,Satchi-Fainaro R

    更新日期：2011-05-01 00:00:00