Abstract:
:The efficient delivery of short interfering RNA (siRNA) is an enormous challenge in the field of gene therapy. Herein, we report a delivery nanosystem based on programmed DNA self-assembly mammalian target of rapamycin (mTOR) siRNA-loaded DNA nanotubes (DNA-NTs). We demonstrate that these siRNA-DNA-NTs can be effectively transfected into pulmonary arterial smooth muscle cells (PASMCs) via endocytosis; and that the loaded mTOR siRNA can induce obvious autophagy and inhibit cell growth under both normal and hypoxic conditions. Moreover, we found that mTOR siRNA can control the autophagy and proliferation of PASMCs under hypoxic condition, suggesting a potential therapeutic application for mTOR siRNA in diseases involving abnormal autophagy in PASMCs.
journal_name
Biomaterialsjournal_title
Biomaterialsauthors
You Z,Qian H,Wang C,He B,Yan J,Mao C,Wang Gdoi
10.1016/j.biomaterials.2015.07.015subject
Has Abstractpub_date
2015-10-01 00:00:00pages
137-50eissn
0142-9612issn
1878-5905pii
S0142-9612(15)00591-8journal_volume
67pub_type
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