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Synthetic probes for in vitro purification and in vivo tracking of hepatocytes derived from human pluripotent stem cells.

Abstract：

:Hepatocytes derived from human pluripotent stem cells (hPSCs) are promising candidates for cell therapy and drug discovery. However, it remains challenging to efficiently purify hepatocytes from undesired cell types after differentiation and to accurately monitor grafted cells after transplantation. Indocyanine Green (ICG), an FDA-approved, near-infrared (NIR) dye, has been used for various clinical purposes and is exclusively taken up by hepatocytes. However, ICG has a long emission wavelength (λem > 800 nm) that is beyond the detection range of fluorescence-activated cell sorting (FACS) systems. Moreover, it is easily eliminated from hepatocytes, hindering its application for NIR imaging. Here, we designed and synthesized two different probes based on the properties of ICG; 1) hepatocyte purifying agent (HPA, λem = 562 nm) for in vitro sorting and 2) hepatocyte imaging agent (HIA, λem = 817 nm) for efficient in vivo NIR imaging. We obtained highly enriched populations of hPSC-derived hepatocytes (hPSC-Heps) from various hPSC lines using HPA probe-based FACS purification. In addition, HIA labelling and NIR imaging allowed the direct visualization and tracking of grafted hPSC-Heps in animals with liver injuries. These results demonstrated that these two probes could be used as powerful tools with hPSC-Heps in both cell replacement therapy and drug screening.

journal_name

Biomaterials

journal_title

Biomaterials

authors

Park JY,Han J,Jung HS,Lee G,Kim HJ,Cho GS,Park HJ,Han C,Kim JS,Kim JH

doi

10.1016/j.biomaterials.2019.119431

subject

Has Abstract

pub_date

2019-11-01 00:00:00

pages

119431

eissn

0142-9612

issn

1878-5905

pii

S0142-9612(19)30530-7

journal_volume

222

pub_type

杂志文章

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