Abstract：
:Tissue engineering requires the use of polymeric scaffolds that mimic many roles of extracellular matrices (ECM) in the body. Controlling cell-scaffold interactions is one of the most critical parameters for regulating cell phenotype in tissue engineering, and a peptide with the sequence of RGD has been widely exploited for this purpose. We hypothesized that the spacer arm length of adhesion ligands coupled to synthetic ECMs could be vital for regulation of cell-scaffold interactions. We prepared alginate gels modified with RGD peptides containing varying spacer arm lengths and cultured primary human fibroblasts either on the gels (2-D) or within the gels (3-D). The spacer arm length of the RGD peptides significantly influenced the adhesion and proliferation of fibroblasts in both the 2-D and 3-D studies. We found that a minimum number of four glycine units in the spacer arm was essential for enhanced adhesion and growth of the cells in vitro. An optimal spacer arm length of the RGD peptides was also necessary for minimizing cellular stress responses as determined by analyzing expression of heat shock proteins and Bcl-2 in cultured cells. This approach to controlling cell phenotype using adhesion peptides with various spacer arm lengths could be useful for designing novel scaffolds in tissue engineering applications.
journal_name
Biomaterialsjournal_title
Biomaterialsauthors
Lee JW,Park YJ,Lee SJ,Lee SK,Lee KYdoi
10.1016/j.biomaterials.2010.03.063subject
Has Abstractpub_date
2010-07-01 00:00:00pages
5545-51issue
21eissn
0142-9612issn
1878-5905pii
S0142-9612(10)00447-3journal_volume
31pub_type
杂志文章相关文献
BIOMATERIALS文献大全abstract：:Peel bond strength and tensile bond strength between three polyvinylsiloxane denture soft liners and a heat-cured acrylic resin denture base were measured using two adhesive systems. The soft lining materials differed only in regard of their filler content and compliance. The values of bond strength and mode of failur...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(01)00253-8
更新日期：2002-03-01 00:00:00
abstract：:In the design of parenteral delivery systems the modulation of the biodegradation of a polymer matrix represents a promising strategy to control drug release. We have investigated the degradation of ABA triblock copolymers, consisting of poly(lactide-co-glycolide) A-blocks and poly(oxyethylene) B-blocks, and PLG, poly...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(99)00262-8
更新日期：2000-05-01 00:00:00
abstract：:A cross-linking method for collagen-based biomaterials was developed using the water-soluble carbodiimide 1-ethyl-3-(3-dimethyl aminopropyl)carbodiimide hydrochloride (EDC). Cross-linking using EDC involves the activation of carboxylic acid groups to give O-acylisourea groups, which form cross-links after reaction wit...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/0142-9612(96)81413-x
更新日期：1996-04-01 00:00:00
abstract：:Porous silica gels with high surface areas were prepared from tetraethylothosilicate and polyacrylic acid (PAA) of high molecular weight in acidic media by a sol-gel method. PAA content and ageing temperatures were varied in order to obtain different microstructures. Samples were sintered at temperatures up to 400 deg...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(02)00190-4
更新日期：2002-11-01 00:00:00
abstract：:Rapid prototyping is a novel process for the production of scaffolds of predetermined size and three-dimensional shape. The aim of the study was to determine the feasibility of this technology for producing scaffolds for tissue engineering an aortic valve and the optimal concentration of collagen processed in this man...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2005.12.003
更新日期：2006-05-01 00:00:00
abstract：:As a common receptor for three myelin associated inhibitors, Nogo-66 receptor (NgR) mediates their inhibitory activities on neurite outgrowth in the adult mammalian central nervous system (CNS). Therapeutic vaccination protocol targeting NgR emulsified with Freund's adjuvant (FA) has been used in spinal cord injury (S...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2011.07.009
更新日期：2011-11-01 00:00:00
abstract：:Pancreatic cancer is predicted to be the second leading cause of cancer-related death by 2025. The best chemotherapy only extends survival by an average of 18 weeks. The extensive fibrotic stroma surrounding the tumor curbs therapeutic options as chemotherapy drugs cannot freely penetrate the tumor. RNA interference (...
journal_title：Biomaterials
pub_type： 杂志文章,评审
doi：10.1016/j.biomaterials.2019.119742
更新日期：2020-05-01 00:00:00
abstract：:Skeletal muscle tissue engineering represents an attractive approach to overcome problems associated with autologous transfer of muscle tissue and provides a valid alternative in muscle regeneration enhancement. The aim of this study was to investigate the suitability, as scaffold for skeletal muscle tissue engineerin...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2004.11.035
更新日期：2005-08-01 00:00:00
abstract：:In human body ascorbic acid plays an essential role in the synthesis and function of skeletal tissues and immune system factors. Ascorbic acid is also a major physiological antioxidant, repairing oxidatively damaged biomolecules, preventing the formation of excessive reactive oxygen species or scavenging these species...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2006.01.036
更新日期：2006-06-01 00:00:00
abstract：:Direct delivery of proteins to the cytosol of cells holds tremendous potential in biological and medical applications. Engineering vehicles for escorting proteins to the cytosol in a controlled release fashion has thus generated considerable interest. We report here the preparation of redox-responsive single-protein n...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2011.03.060
更新日期：2011-08-01 00:00:00
abstract：:A series of novel cationic star polymers were synthesized by conjugating multiple oligoethylenimine (OEI) arms onto an alpha-cyclodextrin (alpha-CD) core as nonviral gene delivery vectors. The molecular structures of the alpha-CD-OEI star polymers, which contained linear or branched OEI arms with different chain lengt...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2007.03.033
更新日期：2007-07-01 00:00:00
abstract：OBJECTIVES:To analyze the biocompatibility of multifilament polyester (PET) meshes used for the implantation of auditory brainstem implants in a standardized Wistar rat model (n=29). METHODS:The physical properties of the meshes were examined during surgery. Using a modified plastic embedding, the local tissue reactio...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(02)00320-4
更新日期：2003-02-01 00:00:00
abstract：:A range of poly(alkyl methacrylate)s in the range C5 to C13 with varying degrees of crosslinking, have been studied with respect to stress-strain behaviour. Where the extensions to break were sufficiently high, stress-strain properties conformed well to the statistical theory of rubber elasticity, the Mooney/Rivlin C2...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/0142-9612(87)90011-1
更新日期：1987-09-01 00:00:00
abstract：:In ophthalmology, there is a need for novel degradable biomaterials for e.g. controlled drug release in the vitreous body. These degradable materials should feature both excellent biocompatibility, and well-defined kinetics of degradation. In most cases, poly(D,L-lactic acid), or poly(lactic-co-glycolic acid) are used...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(99)00223-9
更新日期：2000-03-01 00:00:00
abstract：:Mononuclear phagocyte system is the first target for injected nanomaterials. Monocytes/macrophages are the central actors for trafficking and clearance of magnetic nanoparticles in vivo. However the fate of nanosized magnetic label is an ongoing issue. Here we demonstrate that the monocyte/macrophage system shows a co...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2010.05.062
更新日期：2010-09-01 00:00:00
abstract：:The construction of tissue-engineered devices for medical applications is now possible in vitro using cell culture and bioreactors. Although methods of incorporating them back into the host are available, current constructs depend purely on diffusion which limits their potential. The absence of a vascular network capa...
journal_title：Biomaterials
pub_type： 杂志文章,评审
doi：10.1016/j.biomaterials.2004.07.006
更新日期：2005-05-01 00:00:00
abstract：:The development of highly funtional and orally available nanoparticles is the ultimate goal in nanoparticle delivery. Various functional nanoparticles have been studied to that end but there has yet to be an oral nanoparticle that can be successfully applied. Here, we describe for the first time a novel bile acid conj...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2017.09.022
更新日期：2017-12-01 00:00:00
abstract：:DNA that encodes tumor-specific antigens represents potential immunostimulatory agents. However, rapid enzymatic degradation and fragmentation of DNA during administration can result in limited vector expression and, consequently, poor efficacy. These challenges have necessitated the use of novel strategies for DNA de...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2015.02.125
更新日期：2015-01-01 00:00:00
abstract：:Bioactive hydrogels formed from the Michael-type addition reactions of end-functionalized poly (ethylene glycol) macromers with thiol-containing protease-sensitive peptide crosslinkers have previously been described as matrices for cell-induced enzymatic remodeling. In this study, we sought to develop materials formul...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2010.10.016
更新日期：2011-02-01 00:00:00
abstract：:An examination has been made of factors affecting the results obtained from the impinging jet method for evaluating the durability of dental cements. These included intrinsic factors i.e. the powder/liquid ratio used in the mix and the cure time of the cement and extrinsic factors i.e. the pH, ionic strength and tempe...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/0142-9612(86)90106-7
更新日期：1986-05-01 00:00:00
abstract：:The physiological activity of hyaluronic acid (HA) polymers and oligomers makes it a promising material for a variety of applications. The development of HA-hydrogel scaffolds with improved mechanical stability against degradation and biochemical functionality may enhance their application to tissue engineering. In th...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2004.02.067
更新日期：2005-02-01 00:00:00
abstract：:We report the synthesis and characterization of dendrimer-entrapped gold nanoparticles (Au DENPs) modified by polyethylene glycol (PEG) with enhanced biocompatibility for computed tomography (CT) imaging applications. In this study, amine-terminated poly(amidoamine) dendrimers of generation 5 (G5.NH(2)) modified by PE...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2011.10.052
更新日期：2012-02-01 00:00:00
abstract：:Here we document the complete process of bioresorption and bone replacement of rods made of forged composites of unsintered hydroxyapatite particles/poly l-lactide (F-u-HA/PLLA) implanted in the femoral medullary cavities of rabbits. Bioresorption, osteoconductive bioactivity and bone replacement were compared in thre...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2005.02.016
更新日期：2005-09-01 00:00:00
abstract：:A variety of bioactive composites have been investigated over the last two decades as substitute materials for diseased or damaged tissues in the human body. In this paper, the rationale and strategy of developing these composites are given. Major factors influencing the production and performance of bioactive composi...
journal_title：Biomaterials
pub_type： 杂志文章,评审
doi：10.1016/s0142-9612(03)00037-1
更新日期：2003-06-01 00:00:00
abstract：:The continuous release of nitric oxide (NO) by the native endothelium of blood vessels plays a substantial role in the cardiovascular physiology, as it influences important pathways of cardiovascular homeostasis, inhibits vascular smooth muscle cell (VSMC) proliferation, inhibits platelet activation and aggregation, a...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2015.06.016
更新日期：2015-09-01 00:00:00
abstract：:Many joint diseases are associated with the deposition of crystals in articular cartilage. Three main types are commonly found: calcium pyrophosphate, calcium hydroxyapatite and monosodium urate. The effects of these articular crystals on the frictional properties and the surface condition of the articular cartilage w...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/0142-9612(89)90074-4
更新日期：1989-07-01 00:00:00
abstract：:The clinical application of nanoparticular Gd(III) based contrast agents for tumor molecular MRI has been hindered by safety concerns associated with prolonged tissue retention, although they can produce strong tumor enhancement. In this study, a targeted well-defined cyclodextrin-based nanoglobular contrast agent was...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2016.02.002
更新日期：2016-04-01 00:00:00
abstract：:In the present study, we evaluated, in mice, the efficacy of the tetrafunctional block copolymer 704 as a nonviral gene delivery vector to the lungs. SPECT/CT molecular imaging of gene expression, biochemical assays, and immunohistochemistry were used. Our dataset shows that the formulation 704 resulted in higher leve...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2014.12.051
更新日期：2015-03-01 00:00:00
abstract：:An advantage of using vesicles (liposomes) as drug delivery carriers is that their pharmacokinetics can be controlled by surface characteristics, which can permit specific delivery of the encapsulated agents to organs or cells in vivo. Here we report a vesicle formulation which targets the bone marrow after intravenou...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2007.01.041
更新日期：2007-06-01 00:00:00
abstract：:Clinically used contrast agents for magnetic resonance imaging (MRI) suffer by the lack of specificity; short circulation time; and insufficient relaxivity. Here, a one-step combinatorial approach is described for the synthesis of magnetic lipid-polymer (hybrid) nanoparticles (MHNPs) encapsulating 5 nm ultra-small sup...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2013.07.003
更新日期：2013-10-01 00:00:00
