Abstract:
:Skeletal muscle tissue engineering represents an attractive approach to overcome problems associated with autologous transfer of muscle tissue and provides a valid alternative in muscle regeneration enhancement. The aim of this study was to investigate the suitability, as scaffold for skeletal muscle tissue engineering, of a known biodegradable block copolymer (DegraPol) processed by electrospinning in the novel form of microfibrous membranes. Scaffolds were characterized with reference to their morphological, degradative and mechanical properties. Subsequently, cell viability, adhesion and differentiation on coated and uncoated DegraPol) slides were investigated using line cells (C2C12 and L6) and primary human satellite cells (HSCs). The membranes exhibited absence of toxic residuals and satisfactory mechanical properties (linear elastic behavior up to 10% deformation, E modulus in the order of magnitude of MPa). A promising cellular response was also found in preliminary experiments: both line cells and HSCs adhered, proliferated and fused on differently coated electrospun membranes. Positive staining for myosin heavy chain expression indicated that differentiation of C2C12 multinucleated cells occurred within the porous elastomeric substrate. Together the results of this study provide significant evidence of the suitability of electrospun DegraPol) membranes as scaffolds for skeletal muscle tissue engineering and that they represent a promising alternative to scaffolds currently used in this field.
journal_name
Biomaterialsjournal_title
Biomaterialsauthors
Riboldi SA,Sampaolesi M,Neuenschwander P,Cossu G,Mantero Sdoi
10.1016/j.biomaterials.2004.11.035keywords:
subject
Has Abstractpub_date
2005-08-01 00:00:00pages
4606-15issue
22eissn
0142-9612issn
1878-5905pii
S0142-9612(04)01036-1journal_volume
26pub_type
杂志文章相关文献
BIOMATERIALS文献大全abstract::A surface with covalently bonded heparin was tested in an in vivo model. Heparinized and non-heparinized tubings were inserted in the pig aorta. They were left in place for up to 16 wk. After removal they were analysed for their capacity to take up and inhibit thrombin. A comparison was also made with tubings that had...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/0142-9612(87)90088-3
更新日期:1987-11-01 00:00:00
abstract::Extracellular matrix (ECM) derived scaffolds continue to be investigated for the treatment of volumetric muscle loss (VML) injuries. Clinically, ECM scaffolds have been used for lower extremity VML repair; in particular, MatriStem™, a porcine urinary bladder matrix (UBM), has shown improved functional outcomes and vas...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2015.07.040
更新日期:2015-10-01 00:00:00
abstract::Elucidation of relationship among cellular uptake, localization and biological activities of metal complexes could make great breakthrough in the understanding of their action mechanisms and provide useful information for rational design of metal-based anticancer drugs. Iron(II) complexes have emerged as potential ant...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2015.08.031
更新日期:2015-12-01 00:00:00
abstract::The erosion of composite polymer matrices made of slow and fast eroding polymers was investigated. These matrices can be used as implants that release drugs in a preprogrammed way. To understand the mechanism of drug release, the erosion of cylindrical polymer matrices made of several layers of different polymers was ...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/s0142-9612(96)00151-2
更新日期:1997-03-01 00:00:00
abstract::Tissue engineering generally utilizes natural or synthetic scaffolds to repair or replace damaged tissues. However, due to the lack of guidance of biological signals, most of the implanted scaffolds have always suffered from poor in vivo cellularization. Herein, we demonstrate a bio-orthogonal reaction-based strategy ...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2019.119615
更新日期:2020-02-01 00:00:00
abstract::As a common receptor for three myelin associated inhibitors, Nogo-66 receptor (NgR) mediates their inhibitory activities on neurite outgrowth in the adult mammalian central nervous system (CNS). Therapeutic vaccination protocol targeting NgR emulsified with Freund's adjuvant (FA) has been used in spinal cord injury (S...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2011.07.009
更新日期:2011-11-01 00:00:00
abstract::Alzheimer disease (AD) is a neurodegenerative disorder and the most common form of dementia. Histopathologically is characterized by the presence extracellular neuritic plaques and with a large number of neurons lost. In this paper, we design a new nanomaterial, graphene quantum dots (GQDs) conjugated neuroprotective ...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2016.08.021
更新日期:2016-11-01 00:00:00
abstract:OBJECTIVES:To analyze the biocompatibility of multifilament polyester (PET) meshes used for the implantation of auditory brainstem implants in a standardized Wistar rat model (n=29). METHODS:The physical properties of the meshes were examined during surgery. Using a modified plastic embedding, the local tissue reactio...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/s0142-9612(02)00320-4
更新日期:2003-02-01 00:00:00
abstract::Matrix anisotropy is important for long term in vivo functionality. However, it is not fully understood how to guide matrix anisotropy in vitro. Experiments suggest actin-mediated cell traction contributes. Although F-actin in 2D displays a stretch-avoidance response, 3D data are lacking. We questioned how cyclic stre...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2012.06.103
更新日期:2012-10-01 00:00:00
abstract::The application of poly(hydroxybutyrate-co-hydroxyhexanoate) (PHBHHx) for sustained and controlled delivery of hydrophilic insulin was made possible by preparing insulin phospholipid complex loaded biodegradable PHBHHx nanoparticles (INS-PLC-NPs). The INS-PLC-NPs produced by a solvent evaporation method showed a spher...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2011.10.072
更新日期:2012-02-01 00:00:00
abstract::We compared the osteogenic response in porous alumni (Al) and hydroxyapatite coated porous alumni ceramic (HA/Al) by grafting rat bone marrow cells with these implants subcutaneously in the back of syngeneic rats. The ceramics did not show any bone formation without marrow, but did when combined with marrow cells. The...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/0142-9612(96)89774-2
更新日期:1996-08-01 00:00:00
abstract::Great efforts have been made to develop suitable bioactive constructs that release growth factors (GFs) in a controlled manner for tissue-regeneration applications. Platelet lysates (PLs) are an affordable source of multiple GFs and other proteins, and show great potential in the wound-healing process. Herein, poly-l-...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2020.120138
更新日期:2020-10-01 00:00:00
abstract::Severe wear of the ultra-high molecular weight polyethylene (UHMWPE) components of total joint replacements limits their long-term success. In previous studies, the infrared spectra obtained on retrieved UHMWPE components were interpreted as evidence that the UHMWPE oxidizes in vivo. In direct contrast, infrared spect...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/0142-9612(93)90062-7
更新日期:1993-07-01 00:00:00
abstract::Hydrogels, microgels and nanogels have emerged as versatile and viable platforms for sustained protein release, targeted drug delivery, and tissue engineering due to excellent biocompatibility, a microporous structure with tunable porosity and pore size, and dimensions spanning from human organs, cells to viruses. In ...
journal_title:Biomaterials
pub_type: 杂志文章,评审
doi:10.1016/j.biomaterials.2014.03.001
更新日期:2014-06-01 00:00:00
abstract::There are many ways to influence cell activities, and biomaterials with functional groups attached is an attractive method that clearly has the ability to modulate cell behavior. The evidence is clear that biomaterials, with or without growth factors and cells, have resulted in numerous products for the regenerative m...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2011.02.028
更新日期:2011-06-01 00:00:00
abstract::Silicone elastomers have been used as implants in orthopaedics for replacements of small joints such as the metacarpophalangeal joint. A series of experiments was conducted an axially tensioned silicone elastomer tendon spacer samples to determine whether there is a relationship between stress in the material and imag...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/s0142-9612(97)00155-5
更新日期:1998-01-01 00:00:00
abstract::Notorious bacterial biofilms are becoming severe threats to public health worldwide. As the important component in biofilm extracellular polymeric substances (EPS), extracellular DNA (eDNA) has been manifested to connect different EPS components and bacteria together, leading biofilms hard to eliminate. Herein a serie...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2019.04.007
更新日期:2019-07-01 00:00:00
abstract::New light-activation units equipped with high-illuminant blue light-emitting diodes (LEDs) have recently been proposed as a replacement for the halogen units that are widely used in dentistry to polymerize light-cured resins. The photoinitiators in light-cured dental resins, typified by the camphorquinone (CQ)/amine p...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/s0142-9612(02)00636-1
更新日期:2003-05-01 00:00:00
abstract::Artificial meniscal lesions extending into the avascular part of the meniscus, which do not heal by any other means, were repaired by suturing either a porous polymer implant or a synovial flap into the defect. The implant guided the ingrowth of vascular repair tissue into the defect. This fibrous tissue later on tran...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/0142-9612(91)90066-j
更新日期:1991-11-01 00:00:00
abstract::Tissue-engineering technology employing genetically-modified mesenchymal stem cells combined with proper scaffolds represents a promising strategy for bone regeneration. Elucidating the underlying mechanisms that govern the osteogenesis of mesenchymal stem cells will give deeper insights into the regulatory patterns, ...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2015.10.042
更新日期:2016-01-01 00:00:00
abstract::Hydroxyapatite (HA) in 25%, 50% and 75% w/w fractions was incorporated into chitin solutions and processed into air- and freeze-dried materials. These HA-chitin materials were exposed to cell cultures and implanted into the intramusculature of a rat model. The HA-chitin materials were found to be non-cytotoxic and deg...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/s0142-9612(03)00612-4
更新日期:2004-03-01 00:00:00
abstract::A novel technique has been developed for microstructural studies of bone. The spatial organizations of the mineral and collagen fibres in bone have been a matter of discussion for some time, with numerous diverse observations arising from various preparative techniques. In this latest investigation details of the mine...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/0142-9612(85)90002-x
更新日期:1985-05-01 00:00:00
abstract::Human stem cells are vulnerable to unfavorable conditions, and their transportation relies on costly and inconvenient cryopreservation. We report here that human mesenchymal stem cells (MSC) in spheroids survived ambient conditions (AC) many days longer than in monolayer. Under AC, the viability of MSC in spheroids re...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2017.03.050
更新日期:2017-07-01 00:00:00
abstract::Cell delivery in cell therapy is typically challenged by the low cell survival rate and immunological rejection during cells injection and circulation. Encapsulation of cells with semipermeable hydrogels or membranes can improve cell viability by resisting high shear force and inhibit immune response with the physical...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2019.01.029
更新日期:2019-03-01 00:00:00
abstract::The purpose of this work was to study the relationship between water absorption, dimensional change (swelling) under cavity constraint and radial stress generation in resin matrix dental restorative materials. Water absorption was determined on disc specimens whilst swelling was determined on samples of materials rest...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2003.10.088
更新日期:2004-08-01 00:00:00
abstract::Approximately 285 million people worldwide suffer from diabetes, with insulin supplementation as the most common treatment measure. Regenerative medicine approaches such as a bioengineered pancreas has been proposed as potential therapeutic alternatives. A bioengineered pancreas will benefit from the development of a ...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2013.05.066
更新日期:2013-09-01 00:00:00
abstract::We recently showed that the highly organized architecture of the corneal stroma could be reproduced using scaffolds consisting of orthogonally aligned multilayers of collagen fibrils prepared using a high magnetic field. Here we show that such scaffolds permit the reconstruction in vitro of human hemi-corneas (stroma ...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2010.07.066
更新日期:2010-11-01 00:00:00
abstract::The successful delivery of anti-cancer drugs relies on the simultaneous capability to actively target a specific location, a sufficient lifetime in the active form in the circulation, and traceability and quantification of drug distribution via in vivo medical imaging. Herein, a highly magnetic nanocarrier (HMNC) comp...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/j.biomaterials.2011.05.047
更新日期:2011-09-01 00:00:00
abstract::This study was designed to examine the adhesion, proliferation, and morphology of chondrocytes on new scaffolds; and to examine these cells histologically for the ability of the chondrocytes to maintain chondrogenic properties after subcutaneous implantation into nude mice. Both 75:25 poly (L-lactide-co-epsilon-caprol...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/s0142-9612(03)00210-2
更新日期:2003-09-01 00:00:00
abstract::A glass of nominal composition (wt%) 17.25 MgO-52.75 3CaO.P2O5-30 SiO2 and a glass-ceramic obtained from it showed surface modifications when immersed in an acellular medium having a composition similar to that of human blood plasma. A (Ca, P)-rich layer, with an approximate Ca/P atomic ratio of 1.7, identified as hyd...
journal_title:Biomaterials
pub_type: 杂志文章
doi:10.1016/0142-9612(95)94146-c
更新日期:1995-07-01 00:00:00