Abstract：
:DNA that encodes tumor-specific antigens represents potential immunostimulatory agents. However, rapid enzymatic degradation and fragmentation of DNA during administration can result in limited vector expression and, consequently, poor efficacy. These challenges have necessitated the use of novel strategies for DNA delivery. Herein, we study the ability of cationic self-assembling peptide hydrogels to encapsulate plasmid DNA, and enhance its immunostimulatory potential in vivo. The effect of network charge on the gel's ability to retain the DNA was assessed employing three gel-forming peptides that vary systematically in formal charge. The peptide HLT2, having a formal charge of +5 at neutral pH, was optimal in encapsulating microgram quantities of DNA with little effect on its rheological properties, allowing its effective syringe delivery in vivo. The plasmid, DNA(TA), encapsulated within these gels encodes for a melanoma-specific gp100 antigen fused to the alarmin protein adjuvant HMGN1. Implantation of DNA(TA)-loaded HLT2 gels into mice resulted in an acute inflammatory response with the presence of polymorphonuclear cells, which was followed by infiltrating macrophages. These cellular infiltrates aid in the processing of encapsulated DNA, promoting increased lymphoproliferation and producing an enhanced immune response mediated by CD4+/IFNγ+ expressing Th1 cells, and complemented by the formation of gp100-specific antibodies.
journal_name
Biomaterialsjournal_title
Biomaterialsauthors
Medina SH,Li S,Howard OM,Dunlap M,Trivett A,Schneider JP,Oppenheim JJdoi
10.1016/j.biomaterials.2015.02.125subject
Has Abstractpub_date
2015-01-01 00:00:00pages
545-53eissn
0142-9612issn
1878-5905pii
S0142-9612(15)00263-Xjournal_volume
53pub_type
杂志文章相关文献
BIOMATERIALS文献大全abstract：:The combined use of natural ECM components and synthetic materials offers an attractive alternative to fabricate hydrogel-based tissue engineering scaffolds to study cell-matrix interactions in three-dimensions (3D). A facile method was developed to modify gelatin with cysteine via a bifunctional PEG linker, thus intr...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2011.09.031
更新日期：2012-01-01 00:00:00
abstract：:The purpose of this research was to develop porous poly(D,L-lactide-co-glycolide) (PLGA) scaffolds from which ascorbate-2-phosphate (AsAP) and dexamethasone (Dex) are continuously released for a month for osteogenesis of mesenchymal stem cells for bone tissue engineering. Porous PLGA matrices containing AsAP and Dex w...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(03)00358-2
更新日期：2003-11-01 00:00:00
abstract：:As a common receptor for three myelin associated inhibitors, Nogo-66 receptor (NgR) mediates their inhibitory activities on neurite outgrowth in the adult mammalian central nervous system (CNS). Therapeutic vaccination protocol targeting NgR emulsified with Freund's adjuvant (FA) has been used in spinal cord injury (S...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2011.07.009
更新日期：2011-11-01 00:00:00
abstract：:This study was undertaken to evaluate the role of collagen matrix to enhance platelet-rich plasma (PRP) effects on pro-inflammatory cytokine-induced arthritic model. We have previously demonstrated the highly regenerative roles of PRP to restore disc degeneration and osteoporosis. In this study, PRP modulated by colla...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2011.05.002
更新日期：2011-09-01 00:00:00
abstract：:Primary stability and an optimized load transfer are assumed to account for an undisturbed osseointegration process of implants. Immediate loaded newly designed titanium dental implants inserted in the mandible of minipigs were used for the characterization of the interfacial area between the implant surface and the s...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2003.08.070
更新日期：2004-05-01 00:00:00
abstract：:Angiogenesis is a complex multistage process involving multiple factors and numerous cells. The use of the Chorioallantoic Membrane (CAM) assay is well documented as a method to investigate angiogenesis. This technique is ideal for screening samples, but requires an objective analysis technique. The angiogenic respons...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2007.04.042
更新日期：2007-09-01 00:00:00
abstract：:Thrombomodulin (TM) is a newly described endothelial cell-associated protein that functions as a potent natural anticoagulant by converting thrombin from a procoagulant protease to an anticoagulant. In this study, focussing on the application of TM for biomedical materials, recombinant human TM (hTM) was immobilized o...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/0142-9612(94)90041-8
更新日期：1994-08-01 00:00:00
abstract：:Titiania nanotubes have large potential in medical implant applications but their tissue compatibility is still controversial. Since the sterilization methods may impact the biocompatibility of titania nanotubes and be the source of the controversy, we investigate the influence of three commonly used sterilization met...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2009.11.103
更新日期：2010-03-01 00:00:00
abstract：:The aim of this study was to investigate the potential protective role of fullerenol C60(OH)24 on doxorubicin-induced liver toxicity using in vivo (female Sprague-Dawley rats) and in vitro (human hepatocellular carcinoma - HepG2; colorectal adenocarcinoma cell lines - Caco-2) approaches. The first (healthy control) an...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2008.04.048
更新日期：2008-08-01 00:00:00
abstract：:Mucus layer coating the vaginal epithelium represents a barrier for intravaginally delivered recombined adenoviral (rAd) vectors, but it could be overcome by proper polyethylene glycol (PEG) modification. Here we synthesized two cationic PEG derivatives, amino-(EO)n/(AGE)m-Cyss (APCs). The polymers contained neutral l...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2014.05.056
更新日期：2014-09-01 00:00:00
abstract：:Surface sensitive spectroscopies, Auger electron and X-ray photoelectron (XPS), were used to determine changes in titanium oxide composition, oxide stoichiometry, and adsorbed surface species as a function of exposure to human serum in a balanced electrolyte (serum/SIE) and 8.0 mM ethylenediaminetetraacetic acid in a ...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/0142-9612(92)90108-z
更新日期：1992-01-01 00:00:00
abstract：:The physiological activity of hyaluronic acid (HA) polymers and oligomers makes it a promising material for a variety of applications. The development of HA-hydrogel scaffolds with improved mechanical stability against degradation and biochemical functionality may enhance their application to tissue engineering. In th...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2004.02.067
更新日期：2005-02-01 00:00:00
abstract：:The purpose of this study was to investigate the chemical influence of chemo-mechanical caries removal (CMCR) on surface topography of dental ceramic materials. Thirty samples of three different types of ceramics (Vita Omega, Vita Alpha, and Procera AllTitan) were manufactured and used. With an optical profilometer, d...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(02)00141-2
更新日期：2002-10-01 00:00:00
abstract：:Redox-sensitive non-viral delivery systems exploit intracellular reducing environment to improve the efficacy of the delivery of nucleic acids by selectively releasing the cargo in the subcellular space. Bcl-2 overexpression is frequently observed in human cancers and is closely associated with increased resistance to...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2008.03.009
更新日期：2008-06-01 00:00:00
abstract：:A comparative in vitro assessment of 4 types of tubing representative of the materials currently used in cardiopulmonary bypass (CPB) procedures was conducted under static conditions using liquid extracts of the materials or direct contact with fresh human blood or serum. The parameters monitored were biomarkers of co...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(99)00051-4
更新日期：1999-09-01 00:00:00
abstract：:The shelf life of implantable materials has rarely been addressed. We determined whether osteoconductivity of titanium is stable over time. Rat bone marrow-derived osteoblasts were cultured on new titanium disks (immediately after acid-etching), 3-day-old (stored after acid-etching for 3 days in dark ambient condition...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2009.06.040
更新日期：2009-10-01 00:00:00
abstract：:Biomimetic strategies focusing on presenting short bioadhesive oligopeptides, including the arginine-glycine-aspartic acid (RGD) motif present in numerous adhesive proteins, on a non-fouling support have emerged as promising approaches to improve cellular activities and healing responses. Nevertheless, these bio-inspi...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2006.06.027
更新日期：2006-11-01 00:00:00
abstract：:Hydroxyapatite (HAP) cement was implanted in the femur of rats with experimentally induced osteoporosis, and the osteogenic response was studied histomorphologically. Osteoporosis was induced with ovariectomy with either a normal diet or a low-calcium diet. The rats fed a low-calcium diet after ovariectomy showed a de...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(02)00003-0
更新日期：2002-07-01 00:00:00
abstract：:Bioactive glass is used as both a bone filler and as a coating on implants, and has been advocated as a potential osteogenic scaffold for tissue engineering. Rat-derived mesenchymal stem cells (MSCs) show elevated levels of alkaline phosphatase activity when grown on 45S5 bioactive glass as compared to standard tissue...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2007.05.038
更新日期：2007-10-01 00:00:00
abstract：:In-stent restenosis is one of the most serious modes of failure of cardiovascular stent implant. Although drug-eluting stents have been proven to reduce in-stent restenosis, the nonspecific inhibitory effects of anti-proliferative drugs, such as rapamycin, result in delayed re-endothelialization and fatal late stent t...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2016.11.042
更新日期：2017-02-01 00:00:00
abstract：:One of the key challenges for neural tissue engineering is to exploit supporting materials with robust functionalities not only to govern cell-specific behaviors, but also to form functional neural network. The unique electrical and mechanical properties of graphene imply it as a promising candidate for neural interfa...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2013.05.024
更新日期：2013-09-01 00:00:00
abstract：:Calcium phosphates (CaP) and phosphate-based glass (P-glass, xCaO-(0.55-x) Na(2)O-0.45P(2)O(5) composition) composite coatings were obtained on a strong ZrO(2) to improve biocompatibility, the mechanical strength and biological activity. Hydroxyapatite (HA) and P-glass mixed powder slurries were coated on the ZrO(2) s...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2003.10.094
更新日期：2004-08-01 00:00:00
abstract：:The nanoscale materials properties of bone apatite crystals have been implicated in breast cancer bone metastasis and their interactions with extracellular matrix proteins are likely involved. In this study, we used geologic hydroxyapatite (HAP, Ca10(PO4)6(OH)2), closely related to bone apatite, to investigate how HAP...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2016.11.043
更新日期：2017-02-01 00:00:00
abstract：:Soft tissue degradation of the 3 principal amalgam phases have been investigated in relation to their role in the formation of the amalgam tattoo. Each phase, finely powdered, was implanted subcutaneously into the submandibular region of guinea-pigs for periods ranging from 1 week to 1 year. The rates of breakdown wer...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/0142-9612(83)90043-1
更新日期：1983-04-01 00:00:00
abstract：:Effects of squalane-dissolved fullerene-C60 (Sql-fullerene) on macrophage activation and adipose conversion with oxidative stress were studied using an inflammatory adipose-tissue equivalent (ATE) and OP9 mouse stromal preadipocyte-U937 lymphoma cell co-culture systems. Differentiation of OP9 cells was initiated by in...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2010.04.032
更新日期：2010-08-01 00:00:00
abstract：:Subtle changes on the surface of polymeric materials on autoclaving have been ignored so far. An increase in cyclic trimer content on the surface of polyethylene terephthalate on autoclaving is reported. This increase may affect the biocompatibility of polyethylene terephthalate. Quantification of the cyclic trimer wa...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/0142-9612(84)90079-6
更新日期：1984-09-01 00:00:00
abstract：:Two new formulations of bioactive glasses were used as coatings on titanium alloy (TiAl6V4) implants for prosthetic applications in the orthopaedic field. The biocompatibility of these bioglasses, as well as their osteoconductive properties, were assessed by employing primary cultures of human osteoblasts. A nonbioact...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(97)00249-4
更新日期：1998-06-01 00:00:00
abstract：:Islet transplantation is considered the most promising treatment for type 1 diabetes. However, the clinical success is limited by islet dysfunction in long-term culture. In this study, we have utilized the rapid self-gelation and injectability offered by blending of mulberry silk (Bombyx mori) with non-mulberry (Anthe...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2018.09.037
更新日期：2018-12-01 00:00:00
abstract：:The object of this study was to develop a small experimental animal model for a systematic study of the interfacial phenomena between percutaneous implants and skin. Plasma-sprayed and dense hydroxyapatite test implants were inserted into the tibia and dorsum of guinea pigs and into the tibia and on the cranium of rab...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/0142-9612(88)90096-8
更新日期：1988-05-01 00:00:00
abstract：:Three-dimensional gelatin-chondroitin 6 sulphate-hyanuronic acid (gelatin-C6S-HA) biomatrices were used as the scaffold to investigate the phenotypic and molecular expression of basement membrane (BM) and extracellular matrix (ECM) proteins in vitro. The cells were cultured in three different culture conditions: kerat...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2006.05.004
更新日期：2006-10-01 00:00:00
