Abstract:
:Direct delivery of proteins to the cytosol of cells holds tremendous potential in biological and medical applications. Engineering vehicles for escorting proteins to the cytosol in a controlled release fashion has thus generated considerable interest. We report here the preparation of redox-responsive single-protein nanocapsules for intracellular protein delivery. Through in situ interfacial polymerization, the target protein is noncovalently encapsulated into a positively-charged polymeric shell interconnected by disulfide-containing crosslinkers. The dissociation of the polymeric shell under reducing conditions and the subsequent release of protein were confirmed using cell-free assays in the presence of glutathione (GSH). The nanocapsules were demonstrated to be efficiently internalized into the cells and to release the protein in the reducing cytosol. Using the nanocapsule as a vehicle, we showed that active caspase 3 (CP-3) can be delivered and can induce apoptosis in a variety of human cancer cell lines, including HeLa, MCF-7 and U-87 MG. Our approach therefore presents an effective intracellular protein delivery strategy for therapeutic, diagnostic and reprogramming applications.
journal_name
Biomaterialsjournal_title
Biomaterialsauthors
Zhao M,Biswas A,Hu B,Joo KI,Wang P,Gu Z,Tang Ydoi
10.1016/j.biomaterials.2011.03.060subject
Has Abstractpub_date
2011-08-01 00:00:00pages
5223-30issue
22eissn
0142-9612issn
1878-5905pii
S0142-9612(11)00358-9journal_volume
32pub_type
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