Abstract：
:Amyloid plaques, found in characteristically large numbers in specific brain areas of Alzheimer's disease (AD) and Down's Syndrome (DS) patients, are composed of a 41-43 amino acid peptide, A4, derived from a transmembrane glycoprotein, amyloid precursor protein (APP). In transformed cells APP has been shown to be cleaved within the extracellular portion of the A4 region causing the release of 100-120 kDa soluble N-terminal APP products. If this cleavage occurs in human tissue, neither the soluble product nor the remaining 10-12 kDa transmembrane fragment could be further degraded to yield A4. It has been hypothesized that an alternate APP cleavage product containing the intact A4 region is released in increased amounts in AD and DS brain where subsequent extracellular degradation produces the amyloidogenic A4 peptide. In support of this hypothesis, we have found that PC-12 cells maintained in serum-free media with or without additional injurious agents release a 60 kDa protein which has been detected by immunoprecipitation and immunoblot analyses with 9 antisera elicited by 4 distinct peptides within the carboxyl-terminal half of APP. Six of these antisera, elicited by peptides corresponding to the carboxyl-terminal 20 amino acids of APP, or the A4 peptide itself, do not bind the normally released 120 kDa APP product which is detected by 11 other antisera elicited by peptides with the N-terminal portion of APP. Controls in which two 60 kDa-detecting antisera were preabsorbed with the peptides used to elicit them, produced markedly reduced 60 kd bands on immunoblots.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
J Neurosci Resjournal_title
Journal of neuroscience researchauthors
Baskin F,Rosenberg RN,Greenberg BDdoi
10.1002/jnr.490290115subject
Has Abstractpub_date
1991-05-01 00:00:00pages
127-32issue
1eissn
0360-4012issn
1097-4547journal_volume
29pub_type
杂志文章abstract：:Understanding the pathway for amyloid percursor protein (APP) catabolism has become an important line of investigation. APP is a ubiquitous membrane bound protein that is rapidly cleaved at the membrane, yielding a secreted protein identical to protease nexin II and an internalized 11.5 kDa 100 residue C terminal deri...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490330121
更新日期：1992-09-01 00:00:00
abstract：:The growth cone, the motile tip of developing neuronal processes, is considered responsible for the exact guidance of axons and synaptogenesis. High activity of tyrosine kinases in growth cones may contribute to the functions of growth cones. Our previous work revealed that vinculin is one of the endogenous substrates...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490300127
更新日期：1991-09-01 00:00:00
abstract：:Mice heterozygously deficient in the major myelin component P0 are an established model of an inherited neuropathy and show signs of myelin degeneration in motor nerves. Unlike the case in patients, the sensory nerves are only mildly affected in the mouse mutants and do not show features indicative of myelin degenerat...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.10115
更新日期：2002-01-15 00:00:00
abstract：:High levels of the proto-oncogene product, pp60c-src, have been found in developing and adult neural tissues as well as in certain fully mature cells of the hematopoietic lineage, e.g., platelets and myelomonocytes. Adrenal medullary chromaffin cells exhibit characteristics of both types of cells, i.e., they are deriv...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490240107
更新日期：1989-09-01 00:00:00
abstract：:Experiments were conducted with the aid of intracellular microelectrodes to study physiological properties of neuropile glial cells in the central nervous system of the medicinal leech. The results showed significant contributions of both K+ and Cl- ions to the membrane potential. The transmitter substance 5-hydroxytr...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490070108
更新日期：1982-01-01 00:00:00
abstract：:The use of progesterone following brain injury has a controversial history. On one hand, some lab-based models have showed progesterone as being neuroprotective, but on the other, clinical trials have showed quite the opposite. One of many complaints that arose from this discrepancy was the lack of a diverse pool of a...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.24060
更新日期：2018-04-01 00:00:00
abstract：:Glucocorticoid hormones affect gene expression directly at the level of transcription via intracellular receptors that translocate to the nucleus in the presence of steroid. In the brain, two types of high-affinity receptors bind glucocorticoids, the type I, mineralocorticoid receptor and the type II, glucocorticoid r...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490270315
更新日期：1990-11-01 00:00:00
abstract：:The occurrence of type-1 plasminogen activator inhibitor (PAI-1) in human cerebrospinal fluid (CSF) has not previously been reported. As a member of the serpin superfamily of serine protease inhibitors and an acute phase response component, PAI-1 has powerful potential roles in nervous system homeostasis. We have dete...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490340311
更新日期：1993-02-15 00:00:00
abstract：:Oligodendrocytes (OLs) show heterogeneous properties that depend on their location in the central nervous system (CNS). In this regard, the investigation of oligodendrocyte precursor cells (OPCs) derived from human pluripotent stem cells (hPSCs) should be reconsidered, particularly in cases of brain-predominant disord...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.24413
更新日期：2019-07-01 00:00:00
abstract：:To study the sequence of degenerative events possibly associated with cholinergic cell death in Alzheimer's disease, septal cholinergic neurons derived from rat embryonic brains were exposed to chronic excitotoxic stress by glutamate. Counts of choline acetyltransferase (ChAT)-immunopositive neurons and measurement of...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490400608
更新日期：1995-04-15 00:00:00
abstract：:We have previously shown that Na+, K(+)-ATPase activity in hypothalamus is increased after administration of an acute dose of desipramine, a noradrenaline uptake inhibitor (Viola et al., Cell Molec Neurobiol 9:263-271, 1989). In this report the same treatment (10 mg per kg) was applied to evaluate 3H-ouabain binding i...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/(sici)1097-4547(19970101)47:1<77::aid-jnr8
更新日期：1997-01-01 00:00:00
abstract：:A high density of opioid receptor-like 1 (ORL1) receptor (also referred to as NOP receptor) is found in limbic areas and in regions containing monoamines, which are implicated in emotional activity and physiopathology of depression and anxiety. We aimed at defining precisely the localization of ORL1 receptors in dorsa...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.20571
更新日期：2005-08-15 00:00:00
abstract：:Currently, presymtomatic hematopoietic stem and progenitor cell transplantation (HSPCT) is the only therapeutic modality that alleviates Krabbe's disease (KD)-induced central nervous system damage. However, all HSPCT-treated patients exhibit severe deterioration in peripheral nervous system function characterized by m...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.23847
更新日期：2016-11-01 00:00:00
abstract：:Injury to areas of the central nervous system can alter neurotrophin levels, which may influence postlesion neuronal survival and plasticity. To determine if sensorineural hearing loss induces such changes, we used an enzyme-linked immunosorbent assay (ELISA) to measure neurotrophin-3 (NT-3) and brain-derived neurotro...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.20457
更新日期：2005-05-01 00:00:00
abstract：:The present study examined the effects of high-altitude exposure on the pineal gland, the main source of production of melatonin. It was surmised that hypoxia experienced at high altitude, caused by decreased oxygen tension in the ambient air, might lead to some structural alterations in the pineal gland and, hence, a...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.10136
更新日期：2002-02-15 00:00:00
abstract：:Accumulating evidence indicates an immunosuppressive role for CD4(+)CD25(+) regulatory T cells (Tregs) in autoimmune diseases. Although an impaired Treg function in patients with relapsing-remitting multiple sclerosis (RR-MS) has been reported recently, no information is available so far about Treg function in the pro...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.20852
更新日期：2006-06-01 00:00:00
abstract：:We have studied the interaction between several growth factors to promote parasympathetic neuronal survival. Neither insulin nor insulin-like growth factor-I (IGF-I) had any effect on the survival of embryonic day 8 chick ciliary neurons in culture. Similarly, the protein kinase C activator phorbol dibutyrate (PdBu) h...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490280112
更新日期：1991-01-01 00:00:00
abstract：:The expression of GABAB receptors in cultured mouse cerebellar granule cells was investigated in binding experiments using [3H](S,R)-baclofen as well as in functional assessment of the ability of (R)-baclofen to interact with depolarization (15-40 mM KCl) coupled changes in intracellular Ca2+ homeostasis and neurotran...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490390604
更新日期：1994-12-15 00:00:00
abstract：:In this study, the responses of nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) and neuronal nitric oxide synthase (nNOS) activities were quantitatively analyzed at different times in both ipsilateral and contralateral sides of trigeminal nuclei, after unilateral trigeminal muscle nerve transection, i...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.1235
更新日期：2001-11-01 00:00:00
abstract：:CADASIL is a cerebrovascular disease caused by mutations in the NOTCH3 gene. Most mutations result in a gain or loss of cysteine residue in one of the 34 epidermal growth factor-like repeats in the extracellular domain of the Notch3 protein, thus sparing the number of cysteine residues. To date, more than 130 differen...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.21935
更新日期：2009-04-01 00:00:00
abstract：:Nerve growth factor (NGF) has been purified from the guinea pig prostate using a modification of the Bocchini-Angeletti method for isolating 2.5S NGF from mouse submaxillary glands. As with the mouse preparation, guinea pig prostate NGF appears to migrate as a high molecular weight entity at physiological pH. Followin...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490060403
更新日期：1981-01-01 00:00:00
abstract：:Laminin mediates neural adhesion and process formation. A possible signal transduction pathway for laminin was investigated in both NG108-15 and PC12 neuronal cells using radiolabeling studies as well as various stimulators and inhibitors of phosphatases and kinases. Using [32P]-ortho-phosphate, laminin caused a decre...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490270321
更新日期：1990-11-01 00:00:00
abstract：:Seventeen white patients from the Stanford Sleep Disorders Clinic complaining of excessive daytime somnolence (EDS) were selected for restriction-fragment-length polymorphism (RFLP) studies. Fourteen of the patients with clinically diagnosed narcolepsy were seropositive for DR2. RFLP analysis of these patients compare...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490180134
更新日期：1987-01-01 00:00:00
abstract：:We have previously shown in rat primary cultured mesencephalic cells that cyanide induces a high level of oxidative stress and necrotic death. To evaluate the mechanism of the cytotoxicity, the effects of cyanide on intracellular glutathione (GSH) pools and inducible nitric oxide synthase (iNOS)-mediated reactive nitr...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.20998
更新日期：2006-10-01 00:00:00
abstract：:Rapsyn is a protein that interacts with the cytoplasmic face of the nicotinic acetylcholine receptors (AChR) to cluster them within postsynaptic membrane of muscle. Here we show that intracellular AChRs are also affected by rapsyn. When rapsyn was co-transfected with AChR into QT-6 fibroblasts, (125)I-alpha-bungarotox...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4547(20000415)60:2<155::AID-JNR
更新日期：2000-04-15 00:00:00
abstract：:Perinatal hypoxia is a major cause of neurodevelopmental deficits. Neuronal migration patterns are particularly sensitive to perinatal hypoxia/ischemia and are associated with the clinical deficits. The rat model of hypoxia/ischemia at P7 mimics that of perinatal injury in humans. Before assessing the effects of postn...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.21607
更新日期：2008-05-15 00:00:00
abstract：:Recently we demonstrated that a member of the neuregulin-1 (NRG-1) family of growth factors is a mitogen and survival factor for olfactory ensheathing cells (OECs). OECs are specialized glial cells within the olfactory system that are believed to play a role in the continual nerve re-growth of this tissue. OECs share ...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/1097-4547(20000715)61:2<172::AID-JNR8>3.0.
更新日期：2000-07-15 00:00:00
abstract：:The mitochondrial permeability transition (mPT) has been implicated in both excitotoxic and apoptotic neuronal cell death, despite the fact that it has not been previously identified in neurons. To study the mPT in hippocampal neurons, cultures were loaded with the mitochondrial dye JC-1 and observed with confocal and...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4547(19980915)53:6<728::AID-JNR
更新日期：1998-09-15 00:00:00
abstract：:Brain zinc homeostasis is strictly controlled under healthy conditions, indicating the importance of zinc for physiological function in the brain. A part of zinc in the brain exists in the synaptic vesicles, is released from a subclass of glutamatergic neurons (i.e., zincergic neurons), and serves as a signal factor (...
journal_title：Journal of neuroscience research
pub_type： 杂志文章,评审
doi：10.1002/jnr.23385
更新日期：2014-07-01 00:00:00
abstract：:Niemann-Pick C (NPC) disease is an autosomal recessive lipidosis characterized by lysosomal accumulations of unesterified cholesterol. Cultured NPC cells exhibit a defect in the intracellular trafficking of LDL-derived cholesterol that leads to lysosomal accumulations of unesterified cholesterol. We found in a prelimi...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/(sici)1097-4547(19970801)49:3<389::aid-jnr
更新日期：1997-08-01 00:00:00
