Effect of solvents on obligately anaerobic bacteria.

Abstract:

:Growth of Acetobacterium woodii and Clostridium sporogenes was studied in the presence of water-immiscible solvents. Nitrogen purging, vacuum distillation or distillation under nitrogen were all suitable as methods to remove oxygen from the solvents, since growth rates and yields of A. woodii were unaffected in the presence of tetradecane which had been degassed by these methods. Varying the solvent volume from 20% to 80% of the culture volume had little effect on growth rate of A. woodii. A.woodii was relatively sensitive to organic solvents since growth was inhibited by alkanes with logP(octanol/water) values below 7.1. C. sporogenes was less solvent sensitive, since it grew without inhibition when the logP of the solvent was > or = 6.6. Nevertheless, both A. woodii and C. sporogenes were more sensitive to solvent polarity than aerobic bacteria.

journal_name

Anaerobe

journal_title

Anaerobe

authors

Rodriguez Martinez MF,Kelessidou N,Law Z,Gardiner J,Stephens G

doi

10.1016/j.anaerobe.2007.09.006

subject

Has Abstract

pub_date

2008-02-01 00:00:00

pages

55-60

issue

1

eissn

1075-9964

issn

1095-8274

pii

S1075-9964(07)00079-0

journal_volume

14

pub_type

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