A T-RFLP database for the rapid profiling of methanogenic communities in anaerobic digesters.

Abstract:

:We present a simple protocol for the cost- and time-efficient profiling of methanogens based on T-RFLP fingerprinting of mcrA amplicons. Sequence data were compiled from mesophilic lab-scale and full-scale biogas reactors operated under various conditions and fed with various substrates. The database facilitates the rapid identification of methanogens, thus reducing the need of cloning and sequencing.

journal_name

Anaerobe

journal_title

Anaerobe

authors

Bühligen F,Lucas R,Nikolausz M,Kleinsteuber S

doi

10.1016/j.anaerobe.2016.03.013

subject

Has Abstract

pub_date

2016-06-01 00:00:00

pages

114-6

eissn

1075-9964

issn

1095-8274

pii

S1075-9964(16)30027-0

journal_volume

39

pub_type

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