Abstract:
:Linezolid is a novel oxazolidinone antibacterial agent active against staphylococci (including methicillin-resistant strains), enterococci (including vancomycin-resistant strains), streptococci (including penicillin-intermediate and -resistant Streptococcus pneumoniae), and other aerobic and facultative bacteria. The agent has also demonstrated activity against a broad spectrum of Gram-positive and Gram-negative anaerobic bacteria. Previous time-kill assessments have shown linezolid to be generally bacteriostatic against staphylococci and enterococci, and bactericidal against streptococci. In this study, an anaerobic glovebox technique was employed to conduct time-kill assessments for four strains of anaerobic Gram-positive, and seven strains of anaerobic Gram-negative bacteria. The time-kill experiment was performed using Anaerobe Broth medium. The drugs were tested at four-fold the minimum inhibitory concentration (MIC), or at the higher concentration of 8mg/L for linezolid, 2mg/L for clindamycin, and 8mg/L for metronidazole. Samples for viable count were taken at 0, 6, and 24h, and plated using the Bioscience International Autospiral DW. Exposure of samples to the aerobic environment during plating was held to less than 30min. Plates were counted after a 48h anaerobic incubation (37 degrees C). The species tested included Bacteroides fragilis (2), B. distasonis, B. thetaiotaomicron, Fusobacterium nucleatum, F. varium, Prevotella melaninogenica, Clostridium perfringens, Eubacterium lentum and Peptostreptococcus anaerobius (2). The activity of linezolid was compared to that of metronidazole and clindamycin, two standard anti-anaerobe agents. As expected, the control agents were very active in these assays. Metronidazole yielded log(10)CFU/mL reductions of 3.0 or greater for nine of ten strains; clindamycin yielded log(10)CFU/mL reductions of 2.0 or greater for six of 11 strains, and 3.0 or greater for three strains. Linezolid also produced significant in vitro killing in this model achieving log(10)CFU/mL reductions of 2.0 or greater for six of 11 strains, and 3.0 or greater for four strains. The profile of activity was similar to that of clindamycin indicating that additional developmental studies of linezolid with anaerobic bacteria are warranted.
journal_name
Anaerobejournal_title
Anaerobeauthors
Yagi BH,Zurenko GEdoi
10.1016/S1075-9964(03)00004-0subject
Has Abstractpub_date
2003-02-01 00:00:00pages
1-3issue
1eissn
1075-9964issn
1095-8274pii
S1075-9964(03)00004-0journal_volume
9pub_type
杂志文章相关文献
ANAEROBE文献大全abstract::Relationship between clinical severities of periodontal disease and the expression of the associated pathogens serve as good indicators of real time disease activity and progression. A double blind study using Image J software carried out to assess the density of the amplified band for Porphyromonas gingivalis in peri...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2017.10.011
更新日期:2018-02-01 00:00:00
abstract::To culture facultative and strict anaerobic bacteria is a well-established method for analyzing subgingival plaque samples. Micro-IDent and micro-IDent Plus (HAIN Lifescience GmbH, Nehren, Germany) tests are two commercially available rapid PCR-based methods for the identification and quantification of putative period...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2010.02.005
更新日期:2010-06-01 00:00:00
abstract::Staphylococcus aureus is an opportunistic pathogen that can colonize human and animal intestinal tracts, causing certain gastrointestinal diseases. The adherence of enteric pathogens to host intestinal epithelial cells is important for their pathogenesis. In the present study, Lactobacillus salivarius and Lactobacillu...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2012.08.001
更新日期:2012-10-01 00:00:00
abstract::The gastrointestinal tract is a balanced ecosystem that can get out of balance and predisposed to clostridial diseases or other pathological conditions. The objective of the present study was to evaluate the gut microbiota in dairy cows suffering from chronic botulism. Cows were investigated for Clostridium (C.) botul...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2014.03.013
更新日期:2014-06-01 00:00:00
abstract:BACKGROUND CONTEXT:Delayed-onset surgical site infections following spinal instrumentation are uncommon and often present with chronic pain and implant failure. Anaerobic organisms are rarely implicated and identified with difficulty in these infections. PURPOSE:We report a case of vertebral osteomyelitis and epidural...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2015.08.004
更新日期:2015-10-01 00:00:00
abstract::Bacterial strain QZ1 was isolated from sludge of anoxic sulfide-oxidizing (ASO) reactor. Based on 16S rDNA sequence analysis and morphological characteristics, the isolate was identified as Pseudomonas stutzeri. The isolate was found to be a facultative chemolithotroph, using sulfide as electron donor and nitrite as e...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2009.03.009
更新日期:2009-08-01 00:00:00
abstract::Despite extensive research on the epidemiology of pathogenic clostridia in dogs and cats, most published studies focus on a selected animal population and/or a single veterinary medical centre. We assessed the burden of Clostridium perfringens and C. difficile shedding by small animals in 17 veterinary clinics located...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2017.06.023
更新日期:2017-12-01 00:00:00
abstract::The molecular epidemiology of 38 non-duplicate toxigenic Clostridioides (previously Clostridium) difficile isolates from inpatients from a hospital in Brazil during a 6-year period (2012-2017) were investigated by multilocus sequence typing (MLST) and ribotyping. These isolates were classified into 20 sequence types (...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2019.01.010
更新日期:2019-04-01 00:00:00
abstract:OBJECTIVES:To determine the impact of the 2018 introduction of nucleic acid amplification tests (NAATs) for C. difficile detection on the laboratory diagnosis of C. difficile infection (CDI), and the distribution of C. difficile ribotypes. METHODS:A retrospective analysis of five years (2015-2019) of C. difficile diag...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2020.102313
更新日期:2020-12-09 00:00:00
abstract::The purpose of this article is to set out some important considerations on the main emerging antibiotic resistance patterns among anaerobic bacteria. The first point concerns the Bacteroides fragilis group and its resistance to the combination of β-lactam+β-lactamase inhibitor. When there is overproduction of cephalos...
journal_title:Anaerobe
pub_type: 杂志文章,评审
doi:10.1016/j.anaerobe.2010.10.002
更新日期:2010-12-01 00:00:00
abstract::Bifidobacteria are beneficial bacteria for humans. These bacteria are particularly effective at protecting against infectious diseases and modulating the immune response. It was shown that in newborns, the fecal distribution of the colonizing Bifidobacterium species influences the prevalence of allergic diseases. This...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2014.05.006
更新日期:2014-08-01 00:00:00
abstract::Using a chemically-defined medium simulating genital tract secretions, we have shown that pre-adhering Lactobacillus crispatus to Hela epithelial cells reduced cytotoxicity caused by Gardnerella vaginalis. This effect was associated to the expression of vaginolysin and was specific to L. crispatus interference, as oth...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2018.01.014
更新日期:2018-04-01 00:00:00
abstract::Clostridium (Clostridioides) difficile has been identified in humans and a wide range of animal species, but there has been little study of remote animal populations with limited human contact. The objective of this study was to determine the prevalence and types of C. difficile in wild and captive polar bears (Ursus ...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2019.03.013
更新日期:2019-06-01 00:00:00
abstract::Evolution of antibiotic resistance in the anaerobes was reviewed using recent data covering 2000-2013 as compared to previous years. All studies reported growing moxifloxacin resistance in Bacteroides/Parabacteroides spp. in Europe and USA and in Clostridium difficile in Europe. In half or more studies, the resistance...
journal_title:Anaerobe
pub_type: 杂志文章,评审
doi:10.1016/j.anaerobe.2014.05.004
更新日期:2015-02-01 00:00:00
abstract::Fusobacterium nucleatum is a Gram-negative anaerobe, found in a number of different areas of human and animal bodies as part of the resident microbiota. However, it also appears to be involved in polymicrobial infections in such sites. It occurs in the oral cavity where it is a prominent member of various bacterial co...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1006/anae.1997.0140
更新日期:1998-04-01 00:00:00
abstract::Analysis of restriction and modification activities in natural population of Selenomonas ruminantium have revealed the prevalence of CTGCAG (Pst I isoschizomers) recognizing restriction and/or modification systems in these bacteria. Pst I isoschizomeric restriction endonucleases were detected in 4 out of 15 strains te...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1006/anae.1998.0180
更新日期:1999-02-01 00:00:00
abstract::Susceptibility of intestinal bacteria to various antimicrobial agents in vitro, together with levels of those agents achieved in the gut, provides information on the likely impact of the agents on the intestinal flora. Orally administered drugs that are poorly absorbed may be useful for treatment of intestinal infecti...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2004.04.003
更新日期:2004-08-01 00:00:00
abstract::There is concern that the bacterial colony counts present at the time of manufacture and listed on the probiotic package may not be reflective of the numbers viable colonies at the time of purchase and patient consumption thereby diminishing efficacy. We performed a colony count study of three separate samples of five...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2013.10.005
更新日期:2014-02-01 00:00:00
abstract::Prebiotic oligosaccharides are defined by their selective stimulation of growth and/or activity of bacteria in the digestive system in ways claimed to be beneficial for health. However, apart from the short chain fatty acids, little is known about bacterial metabolites created by fermentation of prebiotics, and the si...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2014.05.007
更新日期:2014-08-01 00:00:00
abstract::Cutibacterium (formerly Propionibacterium) acnes is involved in chronic/low-grade pathologies such as sarcoidosis or prosthetic joint infection (PJI). In these diseases, granulomatous structures are frequently observed. In this study, we induced a physiological granulomatous reaction in response to different well-char...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2017.08.014
更新日期:2017-12-01 00:00:00
abstract::Bifidobacteria are part of the human gastrointestinal microbiota and are used as probiotics in functional food products because of their health promoting properties. However, only few data are available on the phenotypic characteristics displayed by human bifidobacteria strain populations. In this study we compared th...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2013.04.005
更新日期:2013-06-01 00:00:00
abstract::Mastoiditis (M) is the most common intratemporal complication of otitis media. The incidence of M has decreased since the advent of antimicrobial agents. In the last decade, however, there has been a marked increased in the incidence of acute M in several communities, sometimes in association with the growing resistan...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2005.03.005
更新日期:2005-10-01 00:00:00
abstract::Recurrent Clostridioides (formerly Clostridium) difficile infection (rCDI) is common, and patients who have had one recurrence are more likely to have multiple recurrences. Frequent recurrences have been associated with increased morbidity and mortality, high healthcare costs, and lower quality of life. In this review...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2019.102098
更新日期:2020-02-01 00:00:00
abstract::Nitrite reductase (cytochrome cd1) was purified to electrophoretic homogeneity from the soluble extract of the marine denitrifying bacterium Pseudomonas nautica strain 617. Cells were anaerobically grown with 10 mM nitrate as final electron acceptor. The soluble fraction was purified by four successive chromatographic...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1006/anae.1995.1021
更新日期:1995-08-01 00:00:00
abstract::Cellulase family and some other glycosyl hydrolases of anaerobic fungi inhabiting the digestive tract of ruminants are believed to form an enzyme complex called cellulosome. Study of the individual component of cellulosome may shed light on understanding the organization of this complex and its functional mechanism. W...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/S1075-9964(03)00087-8
更新日期:2003-06-01 00:00:00
abstract::Banana is a widely consumed fruit, which contains considerable amounts of potential prebiotic indigestible carbohydrates. In our randomised, controlled trial we aimed to evaluate the in vivo prebiotic effect of banana consumption on faecal microbiota. Thirty-four healthy women participated in the study, having Body Ma...
journal_title:Anaerobe
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.anaerobe.2011.03.018
更新日期:2011-12-01 00:00:00
abstract::Eight spindle-shaped bacteria were isolated from clinical samples in Japan and investigated for their taxonomic position. Phylogenetic trees (based on 16S rRNA, rpoB, zinc protease, and gyrB gene sequence comparisons) showed distinct clustering of eight strains with the type strain of Fusobacterium nucleatum and its c...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2021.102323
更新日期:2021-01-27 00:00:00
abstract::The changing epidemiology of Clostridium difficile infection over the past decades presents a significant challenge in the management of C. difficile associated diseases. The gastrointestinal tract microbiota provides colonization resistance against C. difficile, and growing evidence suggests that gut microbial derive...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2017.03.004
更新日期:2017-06-01 00:00:00
abstract::Resistant starch type 3 (RS3) is retrograded starch which is not digested by human starch degrading enzyme, and will thus undergo bacterial degradation in the colon. The main fermentation products are the Short Chain Fatty Acid (SCFA): acetate, propionate and butyrate. SCFA has significant benefit impact on the metabo...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2011.09.007
更新日期:2012-02-01 00:00:00
abstract::Community-associated Clostridium difficile infection (CA-CDI) represents 32% of all CDI cases based on U.S. population-based data. The current epidemic strain, NAP1, is the most prevalent strain causing these infections. Although complications, recurrence and death are uncommon, one-fourth of the CA-CDI patients are h...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2013.01.006
更新日期:2013-12-01 00:00:00