当前位置： SCI文献检索 > ANAEROBE期刊下所有文献 > A combined metabolomic and phylogenetic study reveals putatively prebiotic effects of high molecular weight arabino-oligosaccharides when assessed by in vitro fermentation in bacterial communities derived from humans.

A combined metabolomic and phylogenetic study reveals putatively prebiotic effects of high molecular weight arabino-oligosaccharides when assessed by in vitro fermentation in bacterial communities derived from humans.

Abstract：

:Prebiotic oligosaccharides are defined by their selective stimulation of growth and/or activity of bacteria in the digestive system in ways claimed to be beneficial for health. However, apart from the short chain fatty acids, little is known about bacterial metabolites created by fermentation of prebiotics, and the significance of the size of the oligosaccharides remains largely unstudied. By in vitro fermentations in human fecal microbial communities (derived from six different individuals), we studied the effects of high-mass (HA, >1 kDa), low-mass (LA, <1 kDa) and mixed (BA) sugar beet arabino-oligosaccharides (AOS) as carbohydrate sources. Fructo-oligosaccharides (FOS) were included as reference. The changes in bacterial communities and the metabolites produced in response to incubation with the different carbohydrates were analyzed by quantitative PCR (qPCR) and Liquid Chromatography-Mass Spectrometry (LC-MS), respectively. All tested carbohydrate sources resulted in a significant increase of Bifidobacterium spp. between 1.79 fold (HA) and 1.64 fold (FOS) in the microbial populations after fermentation, and LC-MS analysis suggested that the bifidobacteria contributed to decomposition of the arabino-oligosaccharide structures, most pronounced in the HA fraction, resulting in release of the essential amino acid phenylalanine. Abundance of Lactobacillus spp. correlated with the presence of a compound, most likely a flavonoid, indicating that lactobacilli contribute to release of such health-promoting substances from plant structures. Additionally, the combination of qPCR and LC-MS revealed a number of other putative interactions between intestinal microbes and the oligosaccharides, which contributes to the understanding of the mechanisms behind prebiotic impact on human health.

journal_name

Anaerobe

journal_title

Anaerobe

authors

Sulek K,Vigsnaes LK,Schmidt LR,Holck J,Frandsen HL,Smedsgaard J,Skov TH,Meyer AS,Licht TR

doi

10.1016/j.anaerobe.2014.05.007

subject

Has Abstract

pub_date

2014-08-01 00:00:00

pages

68-77

eissn

1075-9964

issn

1095-8274

pii

S1075-9964(14)00057-2

journal_volume

28

pub_type

杂志文章

相关文献

ANAEROBE文献大全
  • Expanding the repertoire of conservative site-specific recombination in Clostridioides difficile.

    abstract：:Recent genomic analysis of an epidemic ribotype 027 (RT027) Clostridioides difficile strain revealed the presence of several chromosomal site-specific invertible sites hypothesized to control the expression of adjacent genes in a bimodal on-off mode. This process, named phase variation, is thought to enhance phenotypi...

    journal_title：Anaerobe

    pub_type： 杂志文章

    doi：10.1016/j.anaerobe.2019.102073

    authors： Sekulovic O,Bourgeois J,Shen A,Camilli A

    更新日期：2019-12-01 00:00:00

  • Immunization with an alphatoxin variant 121A/91-R212H protects mice against Clostridium perfringens alphatoxin.

    abstract：:As shown previously, a recombinant alphatoxin variant (rAT121A/91) constructed from the naturally occurring Clostridium perfringens mutant strain 121A/91, was devoid of enzymatic (PLC), hemolytic and lethal activity (18). In the present study, the recombinant variant was altered by an oligonucleotide-directed reversio...

    journal_title：Anaerobe

    pub_type： 杂志文章

    doi：10.1016/j.anaerobe.2005.06.003

    authors： Schoepe H,Neubauer A,Schlapp T,Wieler LH,Baljer G

    更新日期：2006-02-01 00:00:00

  • The aerobic and anaerobic microbiology of pustular acne lesions.

    abstract：:Specimens from 32 pustular acne lesions that were inoculated on media supportive for the growth of aerobic and anaerobic bacteria showed bacterial growth. Only aerobic or facultative bacteria were recovered in 15 (47%) specimens, only anaerobic bacteria in 11 (34%) specimens, and mixed aerobic and anaerobic bacteria i...

    journal_title：Anaerobe

    pub_type： 杂志文章

    doi：10.1006/anae.1995.1031

    authors： Brook I,Frazier EH,Cox ME,Yeager JK

    更新日期：1995-12-01 00:00:00

  • Antimicrobial susceptibility profiles of oral Treponema species.

    abstract：:Treponemes occur in the microflora of the dental plaque. Certain Treponema species that are frequently isolated from chronic periodontitis lesions are involved in its initiation and progression. In addition to mechanical instrumentation, antimicrobial agents are used as an adjunctive treatment modality for periodontit...

    journal_title：Anaerobe

    pub_type： 杂志文章

    doi：10.1016/j.anaerobe.2017.10.005

    authors： Okamoto-Shibayama K,Sekino J,Yoshikawa K,Saito A,Ishihara K

    更新日期：2017-12-01 00:00:00

  • Ultrastructure of Clostridium difficile colonies.

    abstract：:The ultrastructural colony architecture of six Clostridium difficile strains belonging to four different ribotypes (001, 027, 078/126 and 036) was studied by scanning electron microscopy (SEM). In 2-day-old colonies several microareas differing in cell length and organization could be differentiated. Some cells at col...

    journal_title：Anaerobe

    pub_type： 杂志文章

    doi：10.1016/j.anaerobe.2013.09.014

    authors： Lipovsek S,Leitinger G,Rupnik M

    更新日期：2013-12-01 00:00:00

  • Clostridium taeniosporum is a close relative of the Clostridium botulinum Group II.

    abstract：:Clostridium taeniosporum is a Gram-positive, anaerobic, rod-shaped non-toxigenic organism isolated from Crimean lake silt. It is unique in forming spores from which about twelve large, flat, ribbon-like appendages emanate. These ribbon-like structures, about 4.5 microm long and 0.45 microm wide, are assembled from sma...

    journal_title：Anaerobe

    pub_type： 杂志文章

    doi：10.1016/j.anaerobe.2008.11.004

    authors： Iyer AV,Blinkova AL,Yang SY,Harrison M,Tepp WH,Jacobson MJ,Johnson EA,Bennett GN,Walker JR

    更新日期：2008-12-01 00:00:00

  • Susceptibility profiles and resistance genes for carbapenems (cfiA) and metronidazole (nim) among Bacteroides species in a Turkish University Hospital.

    abstract：:Sixty-six nonduplicate Bacteroides clinical isolates collected at Marmara University Hospital were tested to investigate carbapenem and metronidazole resistance profiles and to detect the resistance genes (cfiA and nim) and related insertion sequence (IS) elements. The study found that there were no strains resistant ...

    journal_title：Anaerobe

    pub_type： 杂志文章

    doi：10.1016/j.anaerobe.2011.10.004

    authors： Toprak NU,Uzunkaya OD,Sóki J,Soyletir G

    更新日期：2012-02-01 00:00:00

  • The relationship between bifidobacteria and allergic asthma and/or allergic dermatitis: a prospective study of 0-3 years-old children in Turkey.

    abstract：:Bifidobacteria are beneficial bacteria for humans. These bacteria are particularly effective at protecting against infectious diseases and modulating the immune response. It was shown that in newborns, the fecal distribution of the colonizing Bifidobacterium species influences the prevalence of allergic diseases. This...

    journal_title：Anaerobe

    pub_type： 杂志文章

    doi：10.1016/j.anaerobe.2014.05.006

    authors： Akay HK,Bahar Tokman H,Hatipoglu N,Hatipoglu H,Siraneci R,Demirci M,Borsa BA,Yuksel P,Karakullukcu A,Kangaba AA,Sirekbasan S,Aka S,Mamal Torun M,Kocazeybek BS

    更新日期：2014-08-01 00:00:00

  • Antimicrobial susceptibilities of clinical isolates of the anaerobic bacteria which can cause aspiration pneumonia.

    abstract：:Aspiration pneumonia is an infectious disease of the lungs caused by inhalation of saliva or foods, associated with swallowing dysfunction. Therefore, the major causative organisms are oral or gastric bacteria. In this study, we evaluated the antimicrobial susceptibility patterns of the anaerobic bacteria which can ca...

    journal_title：Anaerobe

    pub_type： 杂志文章

    doi：10.1016/j.anaerobe.2019.04.002

    authors： Fujita K,Takata I,Sugiyama H,Suematsu H,Yamagishi Y,Mikamo H

    更新日期：2019-06-01 00:00:00

  • Inhibition of Staphylococcus aureus adherence to Caco-2 cells by lactobacilli and cell surface properties that influence attachment.

    abstract：:Staphylococcus aureus is an opportunistic pathogen that can colonize human and animal intestinal tracts, causing certain gastrointestinal diseases. The adherence of enteric pathogens to host intestinal epithelial cells is important for their pathogenesis. In the present study, Lactobacillus salivarius and Lactobacillu...

    journal_title：Anaerobe

    pub_type： 杂志文章

    doi：10.1016/j.anaerobe.2012.08.001

    authors： Ren D,Li C,Qin Y,Yin R,Li X,Tian M,Du S,Guo H,Liu C,Zhu N,Sun D,Li Y,Jin N

    更新日期：2012-10-01 00:00:00

  • Investigation of Clostridium botulinum group III's mobilome content.

    abstract：:Clostridium botulinum group III is mainly responsible for botulism in animals. It could lead to high animal mortality rates and, therefore, represents a major environmental and economic concern. Strains of this group harbor the botulinum toxin locus on an unstable bacteriophage. Since the release of the first complete...

    journal_title：Anaerobe

    pub_type： 杂志文章

    doi：10.1016/j.anaerobe.2017.12.009

    authors： Woudstra C,Le Maréchal C,Souillard R,Anniballi F,Auricchio B,Bano L,Bayon-Auboyer MH,Koene M,Mermoud I,Brito RB,Lobato FCF,Silva ROS,Dorner MB,Fach P

    更新日期：2018-02-01 00:00:00

  • Factors predictive of severe Clostridium difficile infection depend on the definition used.

    abstract：CONTEXT:Clostridium difficile infection (CDI) produces a variety of clinical presentations ranging from mild diarrhea to severe infection with fulminant colitis, septic shock, and death. Over the past decade, the emergence of the BI/NAP1/027 strain has been linked to higher prevalence and severity of CDI. The guideline...

    journal_title：Anaerobe

    pub_type： 杂志文章

    doi：10.1016/j.anaerobe.2015.08.002

    authors： Khanafer N,Barbut F,Eckert C,Perraud M,Demont C,Luxemburger C,Vanhems P

    更新日期：2016-02-01 00:00:00

  • Lactoferrin and lactoferricin B reduce adhesion and biofilm formation in the intestinal symbionts Bacteroides fragilis and Bacteroides thetaiotaomicron.

    abstract：:Several factors affect the composition of species that inhabit our intestinal tract, including mode of delivery, genetics and nutrition. Antimicrobial peptides and proteins secreted in the gastrointestinal tract are powerful tools against bacteria. Lactoferrin (LF) inhibits the growth of several bacterial species, suc...

    journal_title：Anaerobe

    pub_type： 杂志文章

    doi：10.1016/j.anaerobe.2020.102232

    authors： de Sá Almeida JS,de Oliveira Marre AT,Teixeira FL,Boente RF,Domingues RMCP,de Paula GR,Lobo LA

    更新日期：2020-08-01 00:00:00

  • Evaluation of the routine antimicrobial susceptibility testing results of clinically significant anaerobic bacteria in a Slovenian tertiary-care hospital in 2015.

    abstract：:The aim of our study was to determined antimicrobial susceptibility profiles of 2673 clinically significant anaerobic bacteria belonging to the major genera, isolated in 2015 in a large tertiary-care hospital in Slovenia. The species identification was performed by MALDI-TOF mass spectrometry. Antimicrobial susceptibi...

    journal_title：Anaerobe

    pub_type： 杂志文章

    doi：10.1016/j.anaerobe.2017.04.007

    authors： Jeverica S,Kolenc U,Mueller-Premru M,Papst L

    更新日期：2017-10-01 00:00:00

  • Association between Clostridioides difficile ribotypes, restriction endonuclease analysis types, and toxin gene expression.

    abstract：OBJECTIVES:Clostridioides difficile strains cause severe infection. Previous studies suggested that the virulence of C. difficile is dependent on ribotype; however, this hypothesis is still controversial. We aim to investigate the relationship between ribotypes, restriction endonuclease analysis (REA) types, and toxin ...

    journal_title：Anaerobe

    pub_type： 杂志文章

    doi：10.1016/j.anaerobe.2018.09.002

    authors： Watanabe H,Koizumi Y,Matsumoto A,Asai N,Yamagishi Y,Mikamo H

    更新日期：2018-12-01 00:00:00

  • Biodiversity and fermentative activity of caecal microbial communities in wild and farm rabbits from Spain.

    abstract：:In order to study the microbial caecal ecosystem of wild and domestic rabbits through the fermentation characteristics and concentration and diversity of bacterial and archaeal communities, caecal samples from sixteen wild rabbits (WR) were contrasted with two groups (n = 4) of farm rabbits receiving low (LSF) or high...

    journal_title：Anaerobe

    pub_type： 杂志文章

    doi：10.1016/j.anaerobe.2012.04.004

    authors： Abecia L,Rodríguez-Romero N,Yañez-Ruiz DR,Fondevila M

    更新日期：2012-06-01 00:00:00

  • Establishment of valnemulin susceptibility breakpoint against Clostridium perfringens in rabbits.

    abstract：:Susceptibility breakpoints provide fundamental information for rational administration of antibiotics. The present investigation reports the first valnemulin susceptibility breakpoint (MIC<0.25 μg/mL) against Clostridium perfringens infections in rabbits based on the wild-type cutoff (COWT) and the pharmacokinetics/ph...

    journal_title：Anaerobe

    pub_type： 杂志文章

    doi：10.1016/j.anaerobe.2017.08.006

    authors： Tao MT,Zhou YF,Sun J,Liu YH,Liao XP

    更新日期：2017-12-01 00:00:00

  • Molecular epidemiology of Clostridioides (Clostridium) difficile strains recovered from clinical trials in the US, Canada and Europe from 2006-2009 to 2012-2015.

    abstract：:The prevalence of C. difficile infection (CDI) and severe CDI are influenced by the prevalence of specific C. difficile strains, which are themselves influenced by antimicrobial susceptibility determinants as well as antimicrobial usage patterns. Restriction endonuclease analysis (REA) typing and antimicrobial suscept...

    journal_title：Anaerobe

    pub_type： 杂志文章

    doi：10.1016/j.anaerobe.2018.05.009

    authors： Cheknis A,Johnson S,Chesnel L,Petrella L,Sambol S,Dale SE,Nary J,Sears P,Citron DM,Goldstein EJC,Gerding DN

    更新日期：2018-10-01 00:00:00

  • Effect of the corn silage to grass silage ratio and feed particle size of diets for ruminants on the ruminal Bacteroides-Prevotella community in vitro.

    abstract：:This study examined whether different corn silage to grass silage ratios in ruminant rations and different grinding levels of the feed affect the composition of the ruminal Bacteroides-Prevotella community in vitro. Three diets, composed of 10% soybean meal as well as of different corn silage and grass silage proporti...

    journal_title：Anaerobe

    pub_type： 杂志文章

    doi：10.1016/j.anaerobe.2010.05.002

    authors： Witzig M,Boguhn J,Kleinsteuber S,Fetzer I,Rodehutscord M

    更新日期：2010-08-01 00:00:00

  • Prevalence and characteristics of Clostridium perfringens and Clostridium difficile in dogs and cats attended in diverse veterinary clinics from the Madrid region.

    abstract：:Despite extensive research on the epidemiology of pathogenic clostridia in dogs and cats, most published studies focus on a selected animal population and/or a single veterinary medical centre. We assessed the burden of Clostridium perfringens and C. difficile shedding by small animals in 17 veterinary clinics located...

    journal_title：Anaerobe

    pub_type： 杂志文章

    doi：10.1016/j.anaerobe.2017.06.023

    authors： Álvarez-Pérez S,Blanco JL,Harmanus C,Kuijper EJ,García ME

    更新日期：2017-12-01 00:00:00

  • Inhibition of Clostridium perfringens sporulation by Bacteroides fragilis and short-chain fatty acids.

    abstract：:The effect a common fecal organism, Bacteroides fragilis, has on the sporulation of Clostridium perfringens, an organism linked to some cases of antibiotic associated diarrhea, was examined. Established B. fragilis cultures significantly decreased the number of heat resistant spores formed by C. perfringens ATCC 12915...

    journal_title：Anaerobe

    pub_type： 杂志文章

    doi：10.1016/j.anaerobe.2004.05.006

    authors： Wrigley DM

    更新日期：2004-10-01 00:00:00

  • Effect of solvents on obligately anaerobic bacteria.

    abstract：:Growth of Acetobacterium woodii and Clostridium sporogenes was studied in the presence of water-immiscible solvents. Nitrogen purging, vacuum distillation or distillation under nitrogen were all suitable as methods to remove oxygen from the solvents, since growth rates and yields of A. woodii were unaffected in the pr...

    journal_title：Anaerobe

    pub_type： 杂志文章

    doi：10.1016/j.anaerobe.2007.09.006

    authors： Rodriguez Martinez MF,Kelessidou N,Law Z,Gardiner J,Stephens G

    更新日期：2008-02-01 00:00:00

  • Functional analysis of an feoB mutant in Clostridium perfringens strain 13.

    abstract：:Bacterial pathogens have adopted numerous mechanisms for acquiring iron from host proteins during an infection, including the direct acquisition of ferric iron from heme-associated proteins or from iron-scavenging siderophores. Ferric iron then is transported into the cytosol, where it can be utilized by the bacterial...

    journal_title：Anaerobe

    pub_type： 杂志文章

    doi：10.1016/j.anaerobe.2016.05.005

    authors： Awad MM,Cheung JK,Tan JE,McEwan AG,Lyras D,Rood JI

    更新日期：2016-10-01 00:00:00

  • Emergence of fluoroquinolone-resistant Propionibacterium acnes caused by amino acid substitutions of DNA gyrase but not DNA topoisomerase IV.

    abstract：:With the aim of elucidating the mechanisms of fluoroquinolones resistance in Propionibacterium acnes, we determined the susceptibility of fluoroquinolones in 211 isolates from patients with acne vulgaris. We identified five isolates (2.4%) with reduced susceptibility to nadifloxacin (minimum inhibitory concentration ≥...

    journal_title：Anaerobe

    pub_type： 杂志文章

    doi：10.1016/j.anaerobe.2016.10.012

    authors： Nakase K,Sakuma Y,Nakaminami H,Noguchi N

    更新日期：2016-12-01 00:00:00

  • Biofilm growth kinetics of a monomethylamine producing Alphaproteobacteria strain isolated from an anaerobic reactor.

    abstract：:Industrial fishing effluents are characterized by high loads of protein and sulfate that stimulate the activity of proteolytic and sulfate reducing bacteria during anaerobic digestion. Their metabolic products (NH3 and H2S respectively) have a well-known detrimental effect on the activity of methanogens. Since methyla...

    journal_title：Anaerobe

    pub_type： 杂志文章

    doi：10.1016/j.anaerobe.2009.04.007

    authors： Jopia P,Ruiz-Tagle N,Villagrán M,Sossa K,Pantoja S,Rueda L,Urrutia-Briones H

    更新日期：2010-02-01 00:00:00

  • The human commensal Bacteroides fragilis binds intestinal mucin.

    abstract：:The mammalian gastrointestinal tract harbors a vast microbial ecosystem, known as the microbiota, which benefits host biology. Bacteroides fragilis is an important anaerobic gut commensal of humans that prevents and cures intestinal inflammation. We wished to elucidate aspects of gut colonization employed by B. fragil...

    journal_title：Anaerobe

    pub_type： 杂志文章

    doi：10.1016/j.anaerobe.2011.05.017

    authors： Huang JY,Lee SM,Mazmanian SK

    更新日期：2011-08-01 00:00:00

  • Clinical sources and antimicrobial susceptibility of Prevotella timonensis at the university hospital of Montpellier, France.

    abstract：:We describe 84 clinical isolates of Prevotella timonensis recovered between January 2007 and November 2016 at the University Hospital of Montpellier. They were recovered from a variety of clinical samples, mostly of genital and wound origins. All isolates were isolated from a mixed aerobic and anaerobic microbiota. An...

    journal_title：Anaerobe

    pub_type： 杂志文章

    doi：10.1016/j.anaerobe.2018.01.002

    authors： Aberkane S,Pradel B,Dumont Y,Veloo ACM,Laurens C,Bonzon L,Godreuil S,Marchandin H,Jean-Pierre H

    更新日期：2018-04-01 00:00:00

  • Clinical relevance of Clostridium bacteremia: An 8-year retrospective study.

    abstract：:Clostridium spp. are recovered from 25% of the blood culture positive with anaerobes. However, the clinical relevance of Clostridium bacteremia has been controverted in the literature, particularly for C. perfringens. We aimed to evaluate the clinical relevance of Clostridium bacteremia, either due to C. perfringens o...

    journal_title：Anaerobe

    pub_type： 杂志文章

    doi：10.1016/j.anaerobe.2020.102202

    authors： Stabler S,Titécat M,Duployez C,Wallet F,Loïez C,Bortolotti P,Faure E,Faure K,Kipnis E,Dessein R,Le Guern R

    更新日期：2020-06-01 00:00:00

  • Effect of modified MRS medium on production and purification of antimicrobial peptide ST4SA produced by Enterococcus mundtii.

    abstract：:Highest antimicrobial activity of peptide ST4SA (51,200 AU/mL) was recorded after 14 h of growth in MRS broth with optimal production at pH 6.0 or 6.5. Growth of strain ST4SA in the presence of tryptone, yeast extract, or a combination of the two, yielded 102,400 AU/mL. An increase in production of peptide ST4SA to 10...

    journal_title：Anaerobe

    pub_type： 杂志文章

    doi：10.1016/j.anaerobe.2008.11.002

    authors： Todorov SD,Dicks LM

    更新日期：2009-06-01 00:00:00

  • Reactor performance of a 750 m(3) anaerobic digestion plant: varied substrate input conditions impacting methanogenic community.

    abstract：:A 750 m(3) anaerobic digester was studied over a half year period including a shift from good reactor performance to a reduced one. Various abiotic parameters like volatile fatty acids (VFA) (formic-, acetic-, propionic-, (iso-)butyric-, (iso-)valeric-, lactic acid), total C, total N, NH4 -N, and total proteins, as we...

    journal_title：Anaerobe

    pub_type： 杂志文章

    doi：10.1016/j.anaerobe.2014.03.012

    authors： Wagner AO,Malin C,Lins P,Gstraunthaler G,Illmer P

    更新日期：2014-10-01 00:00:00