Evaluation of the routine antimicrobial susceptibility testing results of clinically significant anaerobic bacteria in a Slovenian tertiary-care hospital in 2015.

Abstract:

:The aim of our study was to determined antimicrobial susceptibility profiles of 2673 clinically significant anaerobic bacteria belonging to the major genera, isolated in 2015 in a large tertiary-care hospital in Slovenia. The species identification was performed by MALDI-TOF mass spectrometry. Antimicrobial susceptibility was determined immediately at the isolation of the strains against: penicillin, co-amoxiclav, imipenem, clindamycin and metronidazole, using gradient diffusion methodology and EUCAST breakpoints. The most frequent anaerobes were Bacteroides fragilis group with 31% (n = 817), Gram positive anaerobic cocci (GPACs) with 22% (n = 589), Prevotella with 14% (n = 313) and Propionibacterium with 8% (n = 225). Metronidazole has retained full activity (100%) against all groups of anaerobic bacteria intrinsically susceptible to it. Co-amoxiclav and imipenem were active against most tested anaerobes with zero or low resistance rates. However, observed resistance to co-amoxiclav (8%) and imipenem (1%) is worrying especially among B. fragilis group isolates. High overall resistance (23%) to clindamycin was detected in our study and was highest among the genera Prevotella, Bacteroides, Parabacteroides, GPACs and Clostridium. Routine testing of antimicrobial susceptibility of clinically relevant anaerobic bacteria is feasible and provides good surveillance data.

journal_name

Anaerobe

journal_title

Anaerobe

authors

Jeverica S,Kolenc U,Mueller-Premru M,Papst L

doi

10.1016/j.anaerobe.2017.04.007

subject

Has Abstract

pub_date

2017-10-01 00:00:00

pages

64-69

eissn

1075-9964

issn

1095-8274

pii

S1075-9964(17)30074-4

journal_volume

47

pub_type

杂志文章

相关文献

ANAEROBE文献大全
  • ent-Copalic acid antibacterial and anti-biofilm properties against Actinomyces naeslundii and Peptostreptococcus anaerobius.

    abstract::Diterpenes are an important class of plant metabolites that can be used in the search for new antibacterial agents. ent-Copalic acid (CA), the major diterpene in Copaifera species exudates, displays several pharmacological properties. This study evaluates the CA antibacterial potential against the anaerobic bacteria P...

    journal_title:Anaerobe

    pub_type: 杂志文章

    doi:10.1016/j.anaerobe.2018.05.013

    authors: Souza MGM,Leandro LF,Moraes TDS,Abrão F,Veneziani RCS,Ambrosio SR,Martins CHG

    更新日期:2018-08-01 00:00:00

  • Lactoferrin and lactoferricin B reduce adhesion and biofilm formation in the intestinal symbionts Bacteroides fragilis and Bacteroides thetaiotaomicron.

    abstract::Several factors affect the composition of species that inhabit our intestinal tract, including mode of delivery, genetics and nutrition. Antimicrobial peptides and proteins secreted in the gastrointestinal tract are powerful tools against bacteria. Lactoferrin (LF) inhibits the growth of several bacterial species, suc...

    journal_title:Anaerobe

    pub_type: 杂志文章

    doi:10.1016/j.anaerobe.2020.102232

    authors: de Sá Almeida JS,de Oliveira Marre AT,Teixeira FL,Boente RF,Domingues RMCP,de Paula GR,Lobo LA

    更新日期:2020-08-01 00:00:00

  • Lactobacillus crispatus represses vaginolysin expression by BV associated Gardnerella vaginalis and reduces cell cytotoxicity.

    abstract::Using a chemically-defined medium simulating genital tract secretions, we have shown that pre-adhering Lactobacillus crispatus to Hela epithelial cells reduced cytotoxicity caused by Gardnerella vaginalis. This effect was associated to the expression of vaginolysin and was specific to L. crispatus interference, as oth...

    journal_title:Anaerobe

    pub_type: 杂志文章

    doi:10.1016/j.anaerobe.2018.01.014

    authors: Castro J,Martins AP,Rodrigues ME,Cerca N

    更新日期:2018-04-01 00:00:00

  • A molecular technique to explore the relationship between Porphyromonas gingivalis and severity of chronic periodontitis: A clinical approach.

    abstract::Relationship between clinical severities of periodontal disease and the expression of the associated pathogens serve as good indicators of real time disease activity and progression. A double blind study using Image J software carried out to assess the density of the amplified band for Porphyromonas gingivalis in peri...

    journal_title:Anaerobe

    pub_type: 杂志文章

    doi:10.1016/j.anaerobe.2017.10.011

    authors: Bagavad Gita J,Aishwarya AB,Pavithra N,Chandrasekaran SC,Ann V George,Gnanamani A

    更新日期:2018-02-01 00:00:00

  • Prokaryote diversity in the rumen of yak (Bos grunniens) and Jinnan cattle (Bos taurus) estimated by 16S rDNA homology analyses.

    abstract::Prokaryote diversity in the rumen of yak (Bos grunniens) and Jinnan cattle (Bos taurus) was estimated by 16S rDNA homology analysis. Two rumen 16S rDNA libraries were constructed. Of the 194 clones in the library of yak rumen, the sequences were mainly clustered to two phyla, low G+C Gram-positive bacteria (LGCGPB, 54...

    journal_title:Anaerobe

    pub_type: 杂志文章

    doi:10.1016/j.anaerobe.2005.02.001

    authors: An D,Dong X,Dong Z

    更新日期:2005-08-01 00:00:00

  • The aerobic and anaerobic microbiology of pustular acne lesions.

    abstract::Specimens from 32 pustular acne lesions that were inoculated on media supportive for the growth of aerobic and anaerobic bacteria showed bacterial growth. Only aerobic or facultative bacteria were recovered in 15 (47%) specimens, only anaerobic bacteria in 11 (34%) specimens, and mixed aerobic and anaerobic bacteria i...

    journal_title:Anaerobe

    pub_type: 杂志文章

    doi:10.1006/anae.1995.1031

    authors: Brook I,Frazier EH,Cox ME,Yeager JK

    更新日期:1995-12-01 00:00:00

  • Comparison of Clostridium difficile minimum inhibitory concentrations obtained using agar dilution vs broth microdilution methods.

    abstract::Due to increasing antibiotic resistance among anaerobic bacteria, routine antimicrobial susceptibility testing is recommended by the Clinical and Laboratory Standards Institute (CLSI). This study compared the minimum inhibitory concentrations (MICs) from 920 Clostridium difficile isolates tested against seven antimicr...

    journal_title:Anaerobe

    pub_type: 杂志文章

    doi:10.1016/j.anaerobe.2017.02.006

    authors: Hastey CJ,Dale SE,Nary J,Citron D,Law JH,Roe-Carpenter DE,Chesnel L

    更新日期:2017-04-01 00:00:00

  • Antimicrobial susceptibilities of clinical isolates of the anaerobic bacteria which can cause aspiration pneumonia.

    abstract::Aspiration pneumonia is an infectious disease of the lungs caused by inhalation of saliva or foods, associated with swallowing dysfunction. Therefore, the major causative organisms are oral or gastric bacteria. In this study, we evaluated the antimicrobial susceptibility patterns of the anaerobic bacteria which can ca...

    journal_title:Anaerobe

    pub_type: 杂志文章

    doi:10.1016/j.anaerobe.2019.04.002

    authors: Fujita K,Takata I,Sugiyama H,Suematsu H,Yamagishi Y,Mikamo H

    更新日期:2019-06-01 00:00:00

  • Development and application of aerobic, chemically defined media for Dysgonomonas.

    abstract::Members of Dysgonomonas are Gram-stain-negative, non-motile, facultatively anaerobic coccobacilli originally described in relation to their isolation from stool and wounds of human patients (CDC group DF-3). More recently, Dysgonomonas have been found to be widely distributed in terrestrial environments and are partic...

    journal_title:Anaerobe

    pub_type: 杂志文章

    doi:10.1016/j.anaerobe.2020.102302

    authors: Bridges CM,Gage DJ

    更新日期:2020-12-01 00:00:00

  • Impact of the introduction of nucleic acid amplification testing on Clostridioides difficile detection and ribotype distribution in Wales.

    abstract:OBJECTIVES:To determine the impact of the 2018 introduction of nucleic acid amplification tests (NAATs) for C. difficile detection on the laboratory diagnosis of C. difficile infection (CDI), and the distribution of C. difficile ribotypes. METHODS:A retrospective analysis of five years (2015-2019) of C. difficile diag...

    journal_title:Anaerobe

    pub_type: 杂志文章

    doi:10.1016/j.anaerobe.2020.102313

    authors: Perry MD,White PL,Morris TE

    更新日期:2020-12-09 00:00:00

  • Effect of modified MRS medium on production and purification of antimicrobial peptide ST4SA produced by Enterococcus mundtii.

    abstract::Highest antimicrobial activity of peptide ST4SA (51,200 AU/mL) was recorded after 14 h of growth in MRS broth with optimal production at pH 6.0 or 6.5. Growth of strain ST4SA in the presence of tryptone, yeast extract, or a combination of the two, yielded 102,400 AU/mL. An increase in production of peptide ST4SA to 10...

    journal_title:Anaerobe

    pub_type: 杂志文章

    doi:10.1016/j.anaerobe.2008.11.002

    authors: Todorov SD,Dicks LM

    更新日期:2009-06-01 00:00:00

  • Performance of BacT/Alert resin-based FN plus bottles compared with BacT/Alert charcoal-based FN bottles for the detection of anaerobes in experimentally seeded blood cultures.

    abstract::Recently new resin-based BacT/Alert FAN Plus bottles containing antibiotic-binding polymeric beads, were introduced as an improvement of the charcoal-based FAN bottles for the recovery of bacteria and fungi. To assess the performance of the novel anaerobic FN plus bottles in the detection of anaerobic organisms, we co...

    journal_title:Anaerobe

    pub_type: 杂志文章

    doi:10.1016/j.anaerobe.2015.07.010

    authors: De Keukeleire S,Wybo I,Emmerechts K,Piérard D

    更新日期:2015-10-01 00:00:00

  • Association between the gut and oral microbiome with obesity.

    abstract::In recent decades, obesity has become one of the most common lifestyle-associated disorders. Obesity is a major contributing factor for several other lifestyles associated disorders such as type 2 diabetes mellitus, hypertension, and cardiovascular disease. Although genetics and lifestyle have been directly implicated...

    journal_title:Anaerobe

    pub_type: 杂志文章,评审

    doi:10.1016/j.anaerobe.2020.102248

    authors: Benahmed AG,Gasmi A,Doşa A,Chirumbolo S,Mujawdiya PK,Aaseth J,Dadar M,Bjørklund G

    更新日期:2020-08-14 00:00:00

  • Molecular characterization and antimicrobial susceptibilities of Clostridium difficile clinical isolates from Victoria, Australia.

    abstract::Some Australian strain types of Clostridium difficile appear unique, highlighting the global diversity of this bacterium. We examined recent and historic local isolates, finding predominantly toxinotype 0 strains, but also toxinotypes V and VIII. All isolates tested were susceptible to vancomycin and metronidazole, wh...

    journal_title:Anaerobe

    pub_type: 杂志文章

    doi:10.1016/j.anaerobe.2015.05.001

    authors: Mackin KE,Elliott B,Kotsanas D,Howden BP,Carter GP,Korman TM,Riley TV,Rood JI,Jenkin GA,Lyras D

    更新日期:2015-08-01 00:00:00

  • Emergence of fluoroquinolone-resistant Propionibacterium acnes caused by amino acid substitutions of DNA gyrase but not DNA topoisomerase IV.

    abstract::With the aim of elucidating the mechanisms of fluoroquinolones resistance in Propionibacterium acnes, we determined the susceptibility of fluoroquinolones in 211 isolates from patients with acne vulgaris. We identified five isolates (2.4%) with reduced susceptibility to nadifloxacin (minimum inhibitory concentration ≥...

    journal_title:Anaerobe

    pub_type: 杂志文章

    doi:10.1016/j.anaerobe.2016.10.012

    authors: Nakase K,Sakuma Y,Nakaminami H,Noguchi N

    更新日期:2016-12-01 00:00:00

  • Propionibacterium acnes biofilm - A sanctuary for Staphylococcus aureus?

    abstract::The purpose of this study was to measure the effect of combined culture of Propionibacterium acnes and Staphylococcus aureus on biofilm formation under different oxygen concentrations. We measured planktonic growth and biofilm formation of P. acnes and S. aureus alone and together under aerobic and anaerobic condition...

    journal_title:Anaerobe

    pub_type: 杂志文章

    doi:10.1016/j.anaerobe.2016.05.014

    authors: Tyner H,Patel R

    更新日期:2016-08-01 00:00:00

  • Bacterial counts from five over-the-counter probiotics: are you getting what you paid for?

    abstract::There is concern that the bacterial colony counts present at the time of manufacture and listed on the probiotic package may not be reflective of the numbers viable colonies at the time of purchase and patient consumption thereby diminishing efficacy. We performed a colony count study of three separate samples of five...

    journal_title:Anaerobe

    pub_type: 杂志文章

    doi:10.1016/j.anaerobe.2013.10.005

    authors: Goldstein EJ,Citron DM,Claros MC,Tyrrell KL

    更新日期:2014-02-01 00:00:00

  • Anaerobes and laboratory automation: Like oil and water?

    abstract::Diagnostic laboratories are urged to take advantage of novel technological advancements to provide standardized and high-throughput information for clinicians; however, total laboratory automation (TLA) has only recently been introduced in clinical microbiology in the last 10-12 years. The introduction of total labora...

    journal_title:Anaerobe

    pub_type: 杂志文章

    doi:10.1016/j.anaerobe.2019.06.009

    authors: Gajdács M

    更新日期:2019-10-01 00:00:00

  • Diversity of Clostridium difficile in pigs and other animals in Slovenia.

    abstract::A study of Clostridium difficile diversity in pigs, calves and horses in Slovenia was conducted. A total of 547 samples were collected and C. difficile was isolated from 247/485 (50.9%) piglet samples, from 4/42 (9.5%) calf samples, and 1/20 (5%) horse samples. The isolates were characterized by toxinotyping, PCR-ribo...

    journal_title:Anaerobe

    pub_type: 杂志文章

    doi:10.1016/j.anaerobe.2009.07.004

    authors: Avbersek J,Janezic S,Pate M,Rupnik M,Zidaric V,Logar K,Vengust M,Zemljic M,Pirs T,Ocepek M

    更新日期:2009-12-01 00:00:00

  • Factors predictive of severe Clostridium difficile infection depend on the definition used.

    abstract:CONTEXT:Clostridium difficile infection (CDI) produces a variety of clinical presentations ranging from mild diarrhea to severe infection with fulminant colitis, septic shock, and death. Over the past decade, the emergence of the BI/NAP1/027 strain has been linked to higher prevalence and severity of CDI. The guideline...

    journal_title:Anaerobe

    pub_type: 杂志文章

    doi:10.1016/j.anaerobe.2015.08.002

    authors: Khanafer N,Barbut F,Eckert C,Perraud M,Demont C,Luxemburger C,Vanhems P

    更新日期:2016-02-01 00:00:00

  • Anaerobic bacteria and antibiotics: What kind of unexpected resistance could I find in my laboratory tomorrow?

    abstract::The purpose of this article is to set out some important considerations on the main emerging antibiotic resistance patterns among anaerobic bacteria. The first point concerns the Bacteroides fragilis group and its resistance to the combination of β-lactam+β-lactamase inhibitor. When there is overproduction of cephalos...

    journal_title:Anaerobe

    pub_type: 杂志文章,评审

    doi:10.1016/j.anaerobe.2010.10.002

    authors: Dubreuil L,Odou MF

    更新日期:2010-12-01 00:00:00

  • In vitro comparative evaluation of the impact of lacto-N-biose I, a major building block of human milk oligosaccharides, on the fecal microbiota of infants.

    abstract::Lacto-N-biose I (LNB) is a potential factor for the selective growth of bifidobacteria. We previously reported that the species of bifidobacteria predominant in infant intestines might use LNB. We aimed to assess the prebiotic properties of LNB in comparison to other oligosaccharides using an in vitro fermentation sys...

    journal_title:Anaerobe

    pub_type: 杂志文章

    doi:10.1016/j.anaerobe.2012.12.007

    authors: Satoh T,Odamaki T,Namura M,Shimizu T,Iwatsuki K,Nishimoto M,Kitaoka M,Xiao JZ

    更新日期:2013-02-01 00:00:00

  • LuxS signaling in Porphyromonas gingivalis-host interactions.

    abstract::Dental plaque is a multispecies biofilm in the oral cavity that significantly influences oral health. The presence of the oral anaerobic pathogen Porphyromonas gingivalis is an important determinant in the development of periodontitis. Direct and indirect interactions between P. gingivalis and the host play a major ro...

    journal_title:Anaerobe

    pub_type: 杂志文章

    doi:10.1016/j.anaerobe.2014.11.011

    authors: Scheres N,Lamont RJ,Crielaard W,Krom BP

    更新日期:2015-10-01 00:00:00

  • Inhibition of Staphylococcus aureus adherence to Caco-2 cells by lactobacilli and cell surface properties that influence attachment.

    abstract::Staphylococcus aureus is an opportunistic pathogen that can colonize human and animal intestinal tracts, causing certain gastrointestinal diseases. The adherence of enteric pathogens to host intestinal epithelial cells is important for their pathogenesis. In the present study, Lactobacillus salivarius and Lactobacillu...

    journal_title:Anaerobe

    pub_type: 杂志文章

    doi:10.1016/j.anaerobe.2012.08.001

    authors: Ren D,Li C,Qin Y,Yin R,Li X,Tian M,Du S,Guo H,Liu C,Zhu N,Sun D,Li Y,Jin N

    更新日期:2012-10-01 00:00:00

  • Fusobacterium watanabei sp. nov. as additional species within the genus Fusobacerium, isolated from human clinical specimens.

    abstract::Eight spindle-shaped bacteria were isolated from clinical samples in Japan and investigated for their taxonomic position. Phylogenetic trees (based on 16S rRNA, rpoB, zinc protease, and gyrB gene sequence comparisons) showed distinct clustering of eight strains with the type strain of Fusobacterium nucleatum and its c...

    journal_title:Anaerobe

    pub_type: 杂志文章

    doi:10.1016/j.anaerobe.2021.102323

    authors: Tomida J,Akiyama-Miyoshi T,Tanaka K,Hayashi M,Kutsuna R,Fujiwara N,Kawamura Y

    更新日期:2021-01-27 00:00:00

  • Detection of periodontopathogenic bacteria in pregnant women by traditional anaerobic culture method and by a commercial molecular genetic method.

    abstract::To culture facultative and strict anaerobic bacteria is a well-established method for analyzing subgingival plaque samples. Micro-IDent and micro-IDent Plus (HAIN Lifescience GmbH, Nehren, Germany) tests are two commercially available rapid PCR-based methods for the identification and quantification of putative period...

    journal_title:Anaerobe

    pub_type: 杂志文章

    doi:10.1016/j.anaerobe.2010.02.005

    authors: Urbán E,Terhes G,Radnai M,Gorzó I,Nagy E

    更新日期:2010-06-01 00:00:00

  • Complete genome sequences and analysis of the Fusobacterium nucleatum subspecies animalis 7-1 bacteriophage ɸFunu1 and ɸFunu2.

    abstract::Fusobacterium nucleatum is a strictly anaerobic, Gram negative bacterial species that has been associated with dental infections, pre-term labor, appendicitis, inflammatory bowel disease, and, more recently, colorectal cancer. The species is unusual in its phenotypic and genotypic heterogeneity, with some strains demo...

    journal_title:Anaerobe

    pub_type: 杂志文章

    doi:10.1016/j.anaerobe.2015.10.013

    authors: Cochrane K,McGuire AM,Priest ME,Abouelleil A,Cerqueira GC,Lo R,Earl AM,Allen-Vercoe E

    更新日期:2016-04-01 00:00:00

  • A new Lactobacillus plantarum strain, TN8, from the gastro intestinal tract of poultry induces high cytokine production.

    abstract::This study aimed to determine the probiotic potential of 100 strains of Lactic Acid Bacteria (LAB) isolated from different intestinal segments of indigenous poultry in Tunisia. The strains were submitted to a battery of standard tests and criteria commonly used for determining their probiotic properties and attributes...

    journal_title:Anaerobe

    pub_type: 杂志文章

    doi:10.1016/j.anaerobe.2012.05.001

    authors: Ben Salah R,Trabelsi I,Ben Mansour R,Lassoued S,Chouayekh H,Bejar S

    更新日期:2012-08-01 00:00:00

  • Detection of resistance genes and susceptibility patterns in Bacteroides and Parabacteroides strains.

    abstract::Susceptibility to five antimicrobials was determined for Bacteroides spp. (n = 52) and Parabacteroides distasonis (n = 8). All isolates were susceptible to metronidazole. The resistance rates to ampicillin, cefoxitin, tetracycline and clindamycin were 98%, 9.6%, 65.3% and 19.2% of the Bacteroides strains, respectively...

    journal_title:Anaerobe

    pub_type: 杂志文章

    doi:10.1016/j.anaerobe.2010.02.003

    authors: Boente RF,Ferreira LQ,Falcão LS,Miranda KR,Guimarães PL,Santos-Filho J,Vieira JM,Barroso DE,Emond JP,Ferreira EO,Paula GR,Domingues RM

    更新日期:2010-06-01 00:00:00

  • Colonisation of Clostridium in the body is restricted to hypoxic and necrotic areas of tumours.

    abstract::The use of gene therapy is one of the most recent molecular strategies for the treatment of cancer. It is essential, however, to have an efficient transfer system by which the desired gene can be delivered to the correct environment. The experiments described in this report investigate apathogenic Clostridium as a pos...

    journal_title:Anaerobe

    pub_type: 杂志文章

    doi:10.1006/anae.1998.0161

    authors: Lambin P,Theys J,Landuyt W,Rijken P,van der Kogel A,van der Schueren E,Hodgkiss R,Fowler J,Nuyts S,de Bruijn E,Van Mellaert L,Anné J

    更新日期:1998-08-01 00:00:00