Abstract:
:In order to study the microbial caecal ecosystem of wild and domestic rabbits through the fermentation characteristics and concentration and diversity of bacterial and archaeal communities, caecal samples from sixteen wild rabbits (WR) were contrasted with two groups (n = 4) of farm rabbits receiving low (LSF) or high (HSF) soluble fibre diets from 28 (weaning) to 51 days of age. DNA was extracted for quantifying bacteria and Archaea by qPCR and for biodiversity analysis of microbial communities by DGGE. Samples from WR had lower caecal pH and ammonia and higher volatile fatty acids concentration than farm animals. Lower acetate and higher butyrate proportions were detected in WR. Bacterial and archaeal DGGE profiles were clearly different between wild and farm rabbits, and diet-affected population of farm rabbits. Similarity index of bacteria was lower than 0.40 among WR, and 0.52 among farm rabbits. In conclusion, caecal fermentation characteristics differ between wild and farm rabbits, which harbour clearly different bacterial and archaeal communities. In farm rabbits, diversity is influenced by the dietary level of soluble fibre.
journal_name
Anaerobejournal_title
Anaerobeauthors
Abecia L,Rodríguez-Romero N,Yañez-Ruiz DR,Fondevila Mdoi
10.1016/j.anaerobe.2012.04.004subject
Has Abstractpub_date
2012-06-01 00:00:00pages
344-9issue
3eissn
1075-9964issn
1095-8274pii
S1075-9964(12)00044-3journal_volume
18pub_type
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