当前位置： SCI文献检索 > ANAEROBE期刊下所有文献 > Use of PCR primers and probes based on the 23S rRNA and internal transcription spacer (ITS) gene sequence for the detection and enumerization of Lactobacillus acidophilus and Lactobacillus plantarum in feed supplements.

Use of PCR primers and probes based on the 23S rRNA and internal transcription spacer (ITS) gene sequence for the detection and enumerization of Lactobacillus acidophilus and Lactobacillus plantarum in feed supplements.

Abstract：

:Novel polymerase chain reaction (PCR) primers designed from the 16S-23S internal transcription spacer (ITS) rRNA and 23S rRNA genes, respectively, were used for the specific detection of Lactobacillus acidophilus and Lactobacillus plantarum. Molecular weights of the PCR products were 221 and 599 bp, respectively. Strains of L. acidophilus and L. plantarum obtained from the culture center, dairy products, infant stool and other samples, could be identified with these PCR primers. DNAs from other lactic acid bacteria (LAB) species including strains of Lactobacillus pentosus which was closely related to L. plantarum, and bacteria species other than LAB, would not generate the false positive results. When this PCR primer set was used for the detection of L. acidophilus and L. plantarum in feed supplement or feed starter samples, reliable results were obtained. Furthermore, when these L. acidophilus or L. plantarum specific primers were used as DNA probes for the colony hybridization of L. acidophilus and L. plantarum, the viable cells of these LAB species in culture and feed supplements or starter products could be identified and enumerized. The method described here thus offers a rapid and economic way to inspect and assure the quality of the feed supplements or fermentation starters.

journal_name

Anaerobe

journal_title

Anaerobe

authors

Tsai CC,Lai CH,Yu B,Tsen HY

doi

10.1016/j.anaerobe.2010.01.003

subject

Has Abstract

pub_date

2010-06-01 00:00:00

pages

270-7

issue

3

eissn

1075-9964

issn

1095-8274

pii

S1075-9964(10)00016-8

journal_volume

16

pub_type

杂志文章

相关文献

ANAEROBE文献大全
  • Clostridium difficile clinical isolates exhibit variable susceptibility and proteome alterations upon exposure to mammalian cationic antimicrobial peptides.

    abstract：:Clostridium difficile is a leading cause of hospital-acquired bacterial infections in the United States, and the increased incidence of recurrent C. difficile infections is particularly problematic. The molecular mechanisms of C. difficile colonization, including its ability to evade host innate immune responses, is p...

    journal_title：Anaerobe

    pub_type： 杂志文章

    doi：10.1016/j.anaerobe.2012.09.004

    authors： McQuade R,Roxas B,Viswanathan VK,Vedantam G

    更新日期：2012-12-01 00:00:00

  • Antimicrobial susceptibilities of clinical isolates of the anaerobic bacteria which can cause aspiration pneumonia.

    abstract：:Aspiration pneumonia is an infectious disease of the lungs caused by inhalation of saliva or foods, associated with swallowing dysfunction. Therefore, the major causative organisms are oral or gastric bacteria. In this study, we evaluated the antimicrobial susceptibility patterns of the anaerobic bacteria which can ca...

    journal_title：Anaerobe

    pub_type： 杂志文章

    doi：10.1016/j.anaerobe.2019.04.002

    authors： Fujita K,Takata I,Sugiyama H,Suematsu H,Yamagishi Y,Mikamo H

    更新日期：2019-06-01 00:00:00

  • Clostridium taeniosporum is a close relative of the Clostridium botulinum Group II.

    abstract：:Clostridium taeniosporum is a Gram-positive, anaerobic, rod-shaped non-toxigenic organism isolated from Crimean lake silt. It is unique in forming spores from which about twelve large, flat, ribbon-like appendages emanate. These ribbon-like structures, about 4.5 microm long and 0.45 microm wide, are assembled from sma...

    journal_title：Anaerobe

    pub_type： 杂志文章

    doi：10.1016/j.anaerobe.2008.11.004

    authors： Iyer AV,Blinkova AL,Yang SY,Harrison M,Tepp WH,Jacobson MJ,Johnson EA,Bennett GN,Walker JR

    更新日期：2008-12-01 00:00:00

  • Taxonomy--General comments and update on taxonomy of Clostridia and Anaerobic cocci.

    abstract：:Since such things as sites of normal carriage, type and severity of infections produced, and antimicrobial susceptibility patterns may vary according to the particular species involved, accurate taxonomy for differentiation of species is important. For example, reclassification of the 'Bacteroides gracilis group' reve...

    journal_title：Anaerobe

    pub_type： 杂志文章

    doi：10.1006/anae.2002.0436

    authors： Finegold SM,Song Y,Liu C

    更新日期：2002-10-01 00:00:00

  • Biofilm growth kinetics of a monomethylamine producing Alphaproteobacteria strain isolated from an anaerobic reactor.

    abstract：:Industrial fishing effluents are characterized by high loads of protein and sulfate that stimulate the activity of proteolytic and sulfate reducing bacteria during anaerobic digestion. Their metabolic products (NH3 and H2S respectively) have a well-known detrimental effect on the activity of methanogens. Since methyla...

    journal_title：Anaerobe

    pub_type： 杂志文章

    doi：10.1016/j.anaerobe.2009.04.007

    authors： Jopia P,Ruiz-Tagle N,Villagrán M,Sossa K,Pantoja S,Rueda L,Urrutia-Briones H

    更新日期：2010-02-01 00:00:00

  • Elucidating the richness of bacterial groups in the gut of Nicobarese tribal community - Perspective on their lifestyle transition.

    abstract：:Lifestyle and dietary habits are crucial features that can alter the gut microbiome of humans. Humans, along with their gut microbes, have coevolved in order to sustain themselves in different environments. They were able to adapt themselves to the dietary sources available in their environment. The relation between h...

    journal_title：Anaerobe

    pub_type： 杂志文章

    doi：10.1016/j.anaerobe.2016.03.002

    authors： Anwesh M,Kumar KV,Nagarajan M,Chander MP,Kartick C,Paluru V

    更新日期：2016-06-01 00:00:00

  • Bacteremia due to a toxin A-negative, B-positive Clostridioides difficile ribotype 017 strain.

    abstract：:Clostridioides difficile is the leading cause of healthcare-associated diarrhea. Although the incidence of C. difficile-associated diarrhea is increasing worldwide, bacteremia due to C. difficile is relatively rare and represents the least frequent extra-colonic manifestation of C. difficile infection. To date, only 6...

    journal_title：Anaerobe

    pub_type：

    doi：10.1016/j.anaerobe.2020.102195

    authors： Kachrimanidou M,Protonotariou E,Kaiafa G,Ζtrieva E,Skoura L,Savopoulos C,Kuijper E

    更新日期：2020-06-01 00:00:00

  • Capnocytophaga spp. infection causing chorioamnionitis: an unusual suspect.

    abstract：:Capnocytophaga spp. are not often considered as a cause of perinatal infection. However, Capnocytophaga spp. are a commensal of the human oral cavity and can be opportunistic pathogens. The present case illustrates the ability of a species from the human oral cavity, C. sputigena, to cause an ascending infection of th...

    journal_title：Anaerobe

    pub_type： 杂志文章

    doi：10.1016/j.anaerobe.2018.07.006

    authors： Felix L,Rosenberg A,Caraballo KA,Taborga DP,Hamula C

    更新日期：2019-10-01 00:00:00

  • Low antibiotic resistance among anaerobic Gram-negative bacteria in periodontitis 5 years following metronidazole therapy.

    abstract：:The objective of this study was to assess antibiotic susceptibility among predominant Gram-negative anaerobic bacteria isolated from periodontitis patients who 5 years prior had been subject to mechanical therapy with or without adjunctive metronidazole. One pooled sample was taken from the 5 deepest sites of each of ...

    journal_title：Anaerobe

    pub_type： 杂志文章,随机对照试验

    doi：10.1016/j.anaerobe.2016.12.009

    authors： Dahlen G,Preus HR

    更新日期：2017-02-01 00:00:00

  • First case report of bacteremia caused by Dysgonomonas mossii.

    abstract：:We here report a case of Dysgonomonas mossii bacteremia with cholangitis. An 85-year-old male patient with recurrent hepatitis B surface antigen-negative/anti-hepatitis C virus-negative hepatocellular carcinoma came to our hospital in poor physical condition. Two sets of blood cultures revealed a positive result for D...

    journal_title：Anaerobe

    pub_type： 杂志文章

    doi：10.1016/j.anaerobe.2018.08.011

    authors： Buya Y,Shigoka T,Tahara H,Uehara Y,Motomura R,Sakurai S,Tanimoto K,Matsumoto T

    更新日期：2018-12-01 00:00:00

  • Real-time PCR quantification of the predominant bacterial divisions in the distal gut of Meishan and Landrace pigs.

    abstract：:Firmicutes and Bacteroidetes are closely related to body fat in humans and mice, which are the two dominant bacterial divisions of gut microbiota in mammals. Here real-time PCR analysis indicated that Meishan pigs had a 34% reduction in percentage Bacteroidetes (P = 0.008) and a significantly lower proportion of Bacte...

    journal_title：Anaerobe

    pub_type： 杂志文章

    doi：10.1016/j.anaerobe.2008.04.001

    authors： Guo X,Xia X,Tang R,Wang K

    更新日期：2008-10-01 00:00:00

  • Adhesive ability means inhibition activities for lactobacillus against pathogens and S-layer protein plays an important role in adhesion.

    abstract：:Eighty-five strains of lactobacillus were isolated from the pig intestine and identified by sequencing analysis based on 16S rRNA gene, from which five lactobacillus strains with high adhesive ability were selected. The inhibition ability of the five lactobacillus strains with or without S-layer proteins against adher...

    journal_title：Anaerobe

    pub_type： 杂志文章

    doi：10.1016/j.anaerobe.2013.06.005

    authors： Zhang W,Wang H,Liu J,Zhao Y,Gao K,Zhang J

    更新日期：2013-08-01 00:00:00

  • Development of EUCAST disk diffusion method for susceptibility testing of the Bacteroides fragilis group isolates.

    abstract：:With the emergence of antibiotic resistance among Bacteroides fragilis group isolates the need of susceptibility testing in routine laboratories is increasing. The aims of the present study were to evaluate the disk diffusion method for susceptibility testing in case of different clinical isolates of Bacteroides spp b...

    journal_title：Anaerobe

    pub_type： 杂志文章

    doi：10.1016/j.anaerobe.2014.10.008

    authors： Nagy E,Justesen US,Eitel Z,Urbán E,ESCMID Study Group on Anaerobic Infection.

    更新日期：2015-02-01 00:00:00

  • Bacterial counts from five over-the-counter probiotics: are you getting what you paid for?

    abstract：:There is concern that the bacterial colony counts present at the time of manufacture and listed on the probiotic package may not be reflective of the numbers viable colonies at the time of purchase and patient consumption thereby diminishing efficacy. We performed a colony count study of three separate samples of five...

    journal_title：Anaerobe

    pub_type： 杂志文章

    doi：10.1016/j.anaerobe.2013.10.005

    authors： Goldstein EJ,Citron DM,Claros MC,Tyrrell KL

    更新日期：2014-02-01 00:00:00

  • Hydrogen sulfide production from subgingival plaque samples.

    abstract：:Periodontitis is a polymicrobial anaerobe infection. Little is known about the dysbiotic microbiota and the role of bacterial metabolites in the disease process. It is suggested that the production of certain waste products in the proteolytic metabolism may work as markers for disease severity. Hydrogen sulfide (H2S) ...

    journal_title：Anaerobe

    pub_type： 杂志文章

    doi：10.1016/j.anaerobe.2014.09.017

    authors： Basic A,Dahlén G

    更新日期：2015-10-01 00:00:00

  • Association between the gut and oral microbiome with obesity.

    abstract：:In recent decades, obesity has become one of the most common lifestyle-associated disorders. Obesity is a major contributing factor for several other lifestyles associated disorders such as type 2 diabetes mellitus, hypertension, and cardiovascular disease. Although genetics and lifestyle have been directly implicated...

    journal_title：Anaerobe

    pub_type： 杂志文章,评审

    doi：10.1016/j.anaerobe.2020.102248

    authors： Benahmed AG,Gasmi A,Doşa A,Chirumbolo S,Mujawdiya PK,Aaseth J,Dadar M,Bjørklund G

    更新日期：2020-08-14 00:00:00

  • Complete genome sequences and analysis of the Fusobacterium nucleatum subspecies animalis 7-1 bacteriophage ɸFunu1 and ɸFunu2.

    abstract：:Fusobacterium nucleatum is a strictly anaerobic, Gram negative bacterial species that has been associated with dental infections, pre-term labor, appendicitis, inflammatory bowel disease, and, more recently, colorectal cancer. The species is unusual in its phenotypic and genotypic heterogeneity, with some strains demo...

    journal_title：Anaerobe

    pub_type： 杂志文章

    doi：10.1016/j.anaerobe.2015.10.013

    authors： Cochrane K,McGuire AM,Priest ME,Abouelleil A,Cerqueira GC,Lo R,Earl AM,Allen-Vercoe E

    更新日期：2016-04-01 00:00:00

  • Fermentation RS3 derived from sago and rice starch with Clostridium butyricum BCC B2571 or Eubacterium rectale DSM 17629.

    abstract：:Resistant starch type 3 (RS3) is retrograded starch which is not digested by human starch degrading enzyme, and will thus undergo bacterial degradation in the colon. The main fermentation products are the Short Chain Fatty Acid (SCFA): acetate, propionate and butyrate. SCFA has significant benefit impact on the metabo...

    journal_title：Anaerobe

    pub_type： 杂志文章

    doi：10.1016/j.anaerobe.2011.09.007

    authors： Purwani EY,Purwadaria T,Suhartono MT

    更新日期：2012-02-01 00:00:00

  • Diversity of Clostridium difficile in pigs and other animals in Slovenia.

    abstract：:A study of Clostridium difficile diversity in pigs, calves and horses in Slovenia was conducted. A total of 547 samples were collected and C. difficile was isolated from 247/485 (50.9%) piglet samples, from 4/42 (9.5%) calf samples, and 1/20 (5%) horse samples. The isolates were characterized by toxinotyping, PCR-ribo...

    journal_title：Anaerobe

    pub_type： 杂志文章

    doi：10.1016/j.anaerobe.2009.07.004

    authors： Avbersek J,Janezic S,Pate M,Rupnik M,Zidaric V,Logar K,Vengust M,Zemljic M,Pirs T,Ocepek M

    更新日期：2009-12-01 00:00:00

  • Lactobacillus crispatus represses vaginolysin expression by BV associated Gardnerella vaginalis and reduces cell cytotoxicity.

    abstract：:Using a chemically-defined medium simulating genital tract secretions, we have shown that pre-adhering Lactobacillus crispatus to Hela epithelial cells reduced cytotoxicity caused by Gardnerella vaginalis. This effect was associated to the expression of vaginolysin and was specific to L. crispatus interference, as oth...

    journal_title：Anaerobe

    pub_type： 杂志文章

    doi：10.1016/j.anaerobe.2018.01.014

    authors： Castro J,Martins AP,Rodrigues ME,Cerca N

    更新日期：2018-04-01 00:00:00

  • In vitro comparative evaluation of the impact of lacto-N-biose I, a major building block of human milk oligosaccharides, on the fecal microbiota of infants.

    abstract：:Lacto-N-biose I (LNB) is a potential factor for the selective growth of bifidobacteria. We previously reported that the species of bifidobacteria predominant in infant intestines might use LNB. We aimed to assess the prebiotic properties of LNB in comparison to other oligosaccharides using an in vitro fermentation sys...

    journal_title：Anaerobe

    pub_type： 杂志文章

    doi：10.1016/j.anaerobe.2012.12.007

    authors： Satoh T,Odamaki T,Namura M,Shimizu T,Iwatsuki K,Nishimoto M,Kitaoka M,Xiao JZ

    更新日期：2013-02-01 00:00:00

  • Ribotypes associated with Clostridium difficile outbreaks in Brazil display distinct surface protein profiles.

    abstract：:Clostridium difficile is a spore-forming anaerobic intestinal pathogen that causes Clostridium difficile infection (CDI). C. difficile is the leading cause of toxin-mediated nosocomial antibiotic-associated diarrhea. The pathogenesis of CDI is attributed to two major virulence factors, TcdA and TcdB toxins, that cause...

    journal_title：Anaerobe

    pub_type： 杂志文章

    doi：10.1016/j.anaerobe.2017.04.001

    authors： Ferreira TG,Moura H,Barr JR,Pilotto Domingues RMC,Ferreira EO

    更新日期：2017-06-01 00:00:00

  • Lactoferrin and lactoferricin B reduce adhesion and biofilm formation in the intestinal symbionts Bacteroides fragilis and Bacteroides thetaiotaomicron.

    abstract：:Several factors affect the composition of species that inhabit our intestinal tract, including mode of delivery, genetics and nutrition. Antimicrobial peptides and proteins secreted in the gastrointestinal tract are powerful tools against bacteria. Lactoferrin (LF) inhibits the growth of several bacterial species, suc...

    journal_title：Anaerobe

    pub_type： 杂志文章

    doi：10.1016/j.anaerobe.2020.102232

    authors： de Sá Almeida JS,de Oliveira Marre AT,Teixeira FL,Boente RF,Domingues RMCP,de Paula GR,Lobo LA

    更新日期：2020-08-01 00:00:00

  • Anaerobic bacteria cultured from the tongue dorsum of subjects with oral malodor.

    abstract：:The bacteria on the dorsum of the tongue are the most frequent cause of oral malodor; however, the bacterial flora of the tongue has not been well defined. Although recent studies have used DNA probes to detect the presence of certain periodontal pathogens, cultural studies have been limited because of the complexity ...

    journal_title：Anaerobe

    pub_type： 杂志文章

    doi：10.1016/S1075-9964(03)00109-4

    authors： Tyrrell KL,Citron DM,Warren YA,Nachnani S,Goldstein EJ

    更新日期：2003-10-01 00:00:00

  • Determination of the Clostridium perfringens-binding site on fibronectin.

    abstract：:The extracellular matrix protein fibronectin (Fn) is known to bind to the surface of Clostridium perfringens cells. Fn is a disulfide-linked homodimer protein, with each Fn polypeptide consisting of three types of repeating modules: 12 type I, 2 type II, and 15-17 type III modules. To determine the epitope on Fn recog...

    journal_title：Anaerobe

    pub_type： 杂志文章

    doi：10.1016/j.anaerobe.2014.11.007

    authors： Katayama S,Tagomori M,Morita N,Yamasaki T,Nariya H,Okada M,Watanabe M,Hitsumoto Y

    更新日期：2015-08-01 00:00:00

  • A T-RFLP database for the rapid profiling of methanogenic communities in anaerobic digesters.

    abstract：:We present a simple protocol for the cost- and time-efficient profiling of methanogens based on T-RFLP fingerprinting of mcrA amplicons. Sequence data were compiled from mesophilic lab-scale and full-scale biogas reactors operated under various conditions and fed with various substrates. The database facilitates the r...

    journal_title：Anaerobe

    pub_type： 杂志文章

    doi：10.1016/j.anaerobe.2016.03.013

    authors： Bühligen F,Lucas R,Nikolausz M,Kleinsteuber S

    更新日期：2016-06-01 00:00:00

  • The first reported case of pelvic inflammatory disease caused by Actinobaculum massiliense.

    abstract：:We report the first case of pelvic inflammatory disease (PID) caused by Actinobaculum massiliense. A 53-year-old woman attended the emergency department with symptoms compatible with a PID episode, finally resolved by intramuscular antibiotic treatment. Actinobaculum sp. was isolated by culture, and A. massiliense was...

    journal_title：Anaerobe

    pub_type： 杂志文章

    doi：10.1016/j.anaerobe.2018.11.006

    authors： Carrillo-Ávila JA,Bonilla-García L,Navarro-Marí JM,Gutiérrez-Fernández J

    更新日期：2019-02-01 00:00:00

  • The association of anaerobic bacteria with infectious mononucleosis.

    abstract：:This review summarizes the information that supports the potential importance of anaerobic bacteria in the pharyngo-tonsillitis (PT) associated with infectious mononucleosis (IM). Evidence supportive of the potential of involvement of bacteria in IM was provided by several studies that showed increased recovery of bac...

    journal_title：Anaerobe

    pub_type： 杂志文章

    doi：10.1016/j.anaerobe.2005.04.004

    authors： Brook I

    更新日期：2005-12-01 00:00:00

  • Anaerobes: a piece in the puzzle for alternative biofuels.

    abstract：:Although much newsprint is devoted to the subject of reducing the United States and other major developed countries dependence on their respective foreign energy sources; the most challenging issues for society is to provide long-term, sustainable energy sources to accommodate the global population as a whole. The pro...

    journal_title：Anaerobe

    pub_type： 杂志文章

    doi：10.1016/j.anaerobe.2011.05.011

    authors： Lawson PA,Allen TD,Caldwell ME,Tanner RS

    更新日期：2011-08-01 00:00:00

  • A molecular technique to explore the relationship between Porphyromonas gingivalis and severity of chronic periodontitis: A clinical approach.

    abstract：:Relationship between clinical severities of periodontal disease and the expression of the associated pathogens serve as good indicators of real time disease activity and progression. A double blind study using Image J software carried out to assess the density of the amplified band for Porphyromonas gingivalis in peri...

    journal_title：Anaerobe

    pub_type： 杂志文章

    doi：10.1016/j.anaerobe.2017.10.011

    authors： Bagavad Gita J,Aishwarya AB,Pavithra N,Chandrasekaran SC,Ann V George,Gnanamani A

    更新日期：2018-02-01 00:00:00