Abstract：
:Eighty-five strains of lactobacillus were isolated from the pig intestine and identified by sequencing analysis based on 16S rRNA gene, from which five lactobacillus strains with high adhesive ability were selected. The inhibition ability of the five lactobacillus strains with or without S-layer proteins against adherence of Escherichia coli K88 and Salmonella enteritidis 50335 to Caco-2 was evaluated in vitro with Lactobacillus rhamnosus GG strain (LGG) as a positive control. In addition, tolerance of lactobacilli to heat, acid, bile, Zn(2+) and Cu(2+) were assessed. All five selected strains, Lactobacillus salivarius ZJ614 (JN981856), Lactobacillus reuteri ZJ616 (JN981858), L. reuteri ZJ617 (JN981859), L. reuteri ZJ621 (JN981863) and L. reuteri ZJ623 (JN981865), showed inhibition against the two pathogens, E. coli K88 and S. enteritidis 50335. L. reuteri ZJ621 showed higher inhibition ability than the others to S. enteritidis 50335 (P < 0.05). Sodium dodecyl sulfate-Polyacrylamide gel electrophoresis (SDS-PAGE) analysis indicated that all five strains had abundant bands with molecular weight ranging from 34 to 130 KDa as well as had a common band of approximately 42 KDa. After treatment with 5 M LiCl to remove S-layer protein, the inhibition activities of the lactobacilli against pathogens decreased significantly (P < 0.05). The results showed that higher adhesive ability means higher inhibition activity for lactobacillus against pathogen, in which S-layer proteins plays an important role.
journal_name
Anaerobejournal_title
Anaerobeauthors
Zhang W,Wang H,Liu J,Zhao Y,Gao K,Zhang Jdoi
10.1016/j.anaerobe.2013.06.005subject
Has Abstractpub_date
2013-08-01 00:00:00pages
97-103eissn
1075-9964issn
1095-8274pii
S1075-9964(13)00095-4journal_volume
22pub_type
杂志文章相关文献
ANAEROBE文献大全abstract：:The changing epidemiology of Clostridium difficile infection over the past decades presents a significant challenge in the management of C. difficile associated diseases. The gastrointestinal tract microbiota provides colonization resistance against C. difficile, and growing evidence suggests that gut microbial derive...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2017.03.004
更新日期：2017-06-01 00:00:00
abstract：:To investigate whether there is a nexus between the microbial diversity level (taxonomic, functional and ecological) and the stress tolerance potential of the microbial community, the impact of different ammonium sources was evaluated. Therefore reactors adapted either to the anaerobic digestions of sugar beet silage ...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2019.01.003
更新日期：2019-04-01 00:00:00
abstract：:Gut fungal-specific PCR primers have been used to selectively amplify the ITS1 region of gut fungal rDNA recovered from faeces of domestic and wild animals to investigate population diversity. Two different gel-based methods are described for separating populations of gut fungal rDNA amplicons, namely (1) denaturing g...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2009.05.003
更新日期：2010-04-01 00:00:00
abstract：:Several studies have suggested the association of disturbed genital tract microbiota with infertility. Our aim was to clarify the influence of sexual intercourse on partner's genital tract microbiota in infertile couples. Seventeen couples were studied, and in 5 men inflammatory prostatitis (IP) was diagnosed. Semen s...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2011.04.015
更新日期：2011-12-01 00:00:00
abstract：:Aspiration pneumonia is an infectious disease of the lungs caused by inhalation of saliva or foods, associated with swallowing dysfunction. Therefore, the major causative organisms are oral or gastric bacteria. In this study, we evaluated the antimicrobial susceptibility patterns of the anaerobic bacteria which can ca...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2019.04.002
更新日期：2019-06-01 00:00:00
abstract：:Proteins play a pivotal role in thermophily. Comparing the molecular properties of homologous proteins from thermophilic and mesophilic bacteria is important for understanding the mechanisms of microbial adaptation to extreme environments. The thermophile Thermoanaerobacter (Thermoanaerobium) brockii and the mesophile...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1006/anae.1997.0083
更新日期：1997-08-01 00:00:00
abstract：:The recognition of the pathogenicity of Cutibacterium acnes in implant-associated infection is not always obvious. In this paper, we aimed to distinguish pathogenic and non-pathogenic C. acnes isolates. To reach this goal, we investigated the clonal complex (CC) of a large collection of C. acnes clinical isolates thro...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2017.04.009
更新日期：2017-10-01 00:00:00
abstract：:The aim of our study was to determined antimicrobial susceptibility profiles of 2673 clinically significant anaerobic bacteria belonging to the major genera, isolated in 2015 in a large tertiary-care hospital in Slovenia. The species identification was performed by MALDI-TOF mass spectrometry. Antimicrobial susceptibi...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2017.04.007
更新日期：2017-10-01 00:00:00
abstract：:We studied the serum bactericidal activity (SBA) of moxifloxacin and levofloxacin against common pathogens associated with complicated intra-abdominal infections. Ten healthy volunteers received a single dose of moxifloxacin (400 mg) and levofloxacin (750 mg) and serum samples were collected at 2, 4, 8, 12, and 24h af...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2007.09.005
更新日期：2008-02-01 00:00:00
abstract：:Clostridioides difficile is a Gram-positive, anaerobic bacterium. It is known that C. difficile is one of the major causes of antibiotic associated diarrhea. The enhanced antibiotic resistance observed in C. difficile is the result of highly resistant spores produced by the bacterium. In Bacillus subtilis, the sin ope...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2019.05.002
更新日期：2019-10-01 00:00:00
abstract：:We investigated the change in chlortetracycline resistance in 360 Escherichia coli strains separated from the feces of piglets fed fermented liquid feed (FLF) in comparison with those fed dry feed (control). The total amount of lactic acid bacteria in feces was 8.77 x 10(8) CFU/g DM at weaning, which increased to 1.23...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2008.07.001
更新日期：2008-10-01 00:00:00
abstract：:Lifestyle and dietary habits are crucial features that can alter the gut microbiome of humans. Humans, along with their gut microbes, have coevolved in order to sustain themselves in different environments. They were able to adapt themselves to the dietary sources available in their environment. The relation between h...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2016.03.002
更新日期：2016-06-01 00:00:00
abstract：:Relationship between clinical severities of periodontal disease and the expression of the associated pathogens serve as good indicators of real time disease activity and progression. A double blind study using Image J software carried out to assess the density of the amplified band for Porphyromonas gingivalis in peri...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2017.10.011
更新日期：2018-02-01 00:00:00
abstract：:Clostridium difficile is the major cause of nosocomial diarrhea. We have previously demonstrated that in southern Taiwan, severe C. difficile-associated diarrhea (CDAD) cases were due to the C. difficile RT 126 strain infection, indicating the arrival of an epidemic C. difficile clone in southern Taiwan. RT126 has a c...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2016.06.002
更新日期：2016-10-01 00:00:00
abstract：:Recent surveys indicate that the majority of toxigenic Clostridium difficile strains isolated in European hospitals belonged to PCR-ribotypes (RTs) different from RT 027 or RT 078. Among these types, RT 018 has been reported in Italy and, more recently, in Korea and Japan. In Italy, strains RT 018 have become predomin...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2016.10.003
更新日期：2016-12-01 00:00:00
abstract：:This manuscript summarizes some of our earlier work on the microbiology of autism subjects' stool specimens, as compared with stools from control subjects. Our most recent data indicating that Desulfovibrio may play an important role in regressive autism is also presented. In addition, we present information on antimi...
journal_title：Anaerobe
pub_type： 杂志文章,评审
doi：10.1016/j.anaerobe.2011.12.018
更新日期：2012-04-01 00:00:00
abstract：:Although much newsprint is devoted to the subject of reducing the United States and other major developed countries dependence on their respective foreign energy sources; the most challenging issues for society is to provide long-term, sustainable energy sources to accommodate the global population as a whole. The pro...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2011.05.011
更新日期：2011-08-01 00:00:00
abstract：:This study examined whether different corn silage to grass silage ratios in ruminant rations and different grinding levels of the feed affect the composition of the ruminal Bacteroides-Prevotella community in vitro. Three diets, composed of 10% soybean meal as well as of different corn silage and grass silage proporti...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2010.05.002
更新日期：2010-08-01 00:00:00
abstract：:Clostridia represent a diverse group of spore-forming gram positive anaerobes that include several pathogenic species. In general, diseases caused by clostridia are a result of intoxication of the infected host. Thus, clostridial toxins have been targeted for diagnostic, therapeutic, and preventive strategies against ...
journal_title：Anaerobe
pub_type： 杂志文章,评审
doi：10.1016/j.anaerobe.2013.08.009
更新日期：2013-12-01 00:00:00
abstract：:We here report a case of Dysgonomonas mossii bacteremia with cholangitis. An 85-year-old male patient with recurrent hepatitis B surface antigen-negative/anti-hepatitis C virus-negative hepatocellular carcinoma came to our hospital in poor physical condition. Two sets of blood cultures revealed a positive result for D...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2018.08.011
更新日期：2018-12-01 00:00:00
abstract：:A 750 m(3) anaerobic digester was studied over a half year period including a shift from good reactor performance to a reduced one. Various abiotic parameters like volatile fatty acids (VFA) (formic-, acetic-, propionic-, (iso-)butyric-, (iso-)valeric-, lactic acid), total C, total N, NH4 -N, and total proteins, as we...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2014.03.012
更新日期：2014-10-01 00:00:00
abstract：:Analysis of restriction and modification activities in natural population of Selenomonas ruminantium have revealed the prevalence of CTGCAG (Pst I isoschizomers) recognizing restriction and/or modification systems in these bacteria. Pst I isoschizomeric restriction endonucleases were detected in 4 out of 15 strains te...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1006/anae.1998.0180
更新日期：1999-02-01 00:00:00
abstract：:Community-associated Clostridium difficile infection (CA-CDI) represents 32% of all CDI cases based on U.S. population-based data. The current epidemic strain, NAP1, is the most prevalent strain causing these infections. Although complications, recurrence and death are uncommon, one-fourth of the CA-CDI patients are h...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2013.01.006
更新日期：2013-12-01 00:00:00
abstract：:The chemotactic behaviour of Campylobacter strains was determined in the presence of different amino acids at two temperatures (37 °C and 42 °C). Two strains of catalase positive (Campylobacter jejuni) and negative (Campylobacter sputurum) Campylobacter were isolated from river water in Tonekabon, Iran and identified ...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2011.06.010
更新日期：2011-12-01 00:00:00
abstract：:Members of Dysgonomonas are Gram-stain-negative, non-motile, facultatively anaerobic coccobacilli originally described in relation to their isolation from stool and wounds of human patients (CDC group DF-3). More recently, Dysgonomonas have been found to be widely distributed in terrestrial environments and are partic...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2020.102302
更新日期：2020-12-01 00:00:00
abstract：:Cutibacterium (formerly Propionibacterium) acnes is involved in chronic/low-grade pathologies such as sarcoidosis or prosthetic joint infection (PJI). In these diseases, granulomatous structures are frequently observed. In this study, we induced a physiological granulomatous reaction in response to different well-char...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2017.08.014
更新日期：2017-12-01 00:00:00
abstract：:Susceptibility breakpoints provide fundamental information for rational administration of antibiotics. The present investigation reports the first valnemulin susceptibility breakpoint (MIC<0.25 μg/mL) against Clostridium perfringens infections in rabbits based on the wild-type cutoff (COWT) and the pharmacokinetics/ph...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2017.08.006
更新日期：2017-12-01 00:00:00
abstract：:This review focuses on the virulence arsenal of the most pathogenic species among Gram positive anaerobic cocci, Finegoldia magna according to recently published data from 2012 to 2016. Virulence factors like sortase dependent pili and F. magna adhesion factor (FAF) facilitate the start of the infection. Albumin bindi...
journal_title：Anaerobe
pub_type： 杂志文章,评审
doi：10.1016/j.anaerobe.2016.10.007
更新日期：2016-12-01 00:00:00
abstract：:Bacteroides thetaiotaomicron maybe one of the most adaptable intestinal bacteria due to its complex genome. Known to be an opportunistic pathogenic anaerobe, B. thetaiotaomicron has recently been described as a symbiont with anti-inflammatory properties. In this study, peptide mass finger printing technique was used t...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2014.04.008
更新日期：2014-08-01 00:00:00
abstract：:Injection drug use (IDU), specifically non-intravenous "skin-popping" of heroin, seems to provide optimal conditions for Clostridial infection and toxin production. IDU is therefore a major risk factor for wound botulism and Clostridial necrotizing soft tissue infections (NSTI) and continues to be linked to cases of t...
journal_title：Anaerobe
pub_type： 杂志文章,评审
doi：10.1016/j.anaerobe.2014.09.005
更新日期：2014-12-01 00:00:00
