Abstract:
:Clostridioides difficile is a Gram-positive, anaerobic bacterium. It is known that C. difficile is one of the major causes of antibiotic associated diarrhea. The enhanced antibiotic resistance observed in C. difficile is the result of highly resistant spores produced by the bacterium. In Bacillus subtilis, the sin operon is involved in sporulation inhibition. Two proteins coded within this operon, SinR and SinI, have an antagonistic relationship; SinR acts as an inhibitor to sporulation whereas SinI represses the activity of SinR, thus allowing the bacterium to sporulate. In a previous study, we examined the sin locus in C. difficile and named the two genes associated with this operon sinR and sinR', analogous to sinR and sinI in B. subtilis, respectively. We have shown that SinR and SinR' have pleiotropic roles in pathogenesis pathways and interact antagonistically with each other. Unlike B. subtilis SinI, SinR' in C. difficile carries two domains: the HTH domain and the Multimerization Domain (MD). In this study, we first performed a GST Pull-down experiment to determine the domain within SinR' that interacts with SinR. Second, the effect of these two domains on three phenotypes; sporulation, motility, and toxin production was examined. The findings of this study confirmed the prediction that the Multimerization Domain (MD) of SinR' is responsible for the interaction between SinR and SinR'. It was also discovered that SinR' regulates sporulation, toxin production and motility primarily by inhibiting SinR activity through the Multimerization Domain (MD).
journal_name
Anaerobejournal_title
Anaerobeauthors
Ciftci Y,Girinathan BP,Dhungel BA,Hasan MK,Govind Rdoi
10.1016/j.anaerobe.2019.05.002subject
Has Abstractpub_date
2019-10-01 00:00:00pages
1-7eissn
1075-9964issn
1095-8274pii
S1075-9964(19)30080-0journal_volume
59pub_type
杂志文章相关文献
ANAEROBE文献大全abstract::The aim of this study was to determine the microbial profile of periodontal lesions in HIV seropositive patients and to compare it with rapidly progressing periodontal lesions in systemically healthy patients. The subgingival microflora of 20 CDC II, 20 CDC III, 20 CDC IV/V and 20 systemically healthy patients with ra...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1006/anae.1997.0081
更新日期:1997-04-01 00:00:00
abstract::Clostridia represent a diverse group of spore-forming gram positive anaerobes that include several pathogenic species. In general, diseases caused by clostridia are a result of intoxication of the infected host. Thus, clostridial toxins have been targeted for diagnostic, therapeutic, and preventive strategies against ...
journal_title:Anaerobe
pub_type: 杂志文章,评审
doi:10.1016/j.anaerobe.2013.08.009
更新日期:2013-12-01 00:00:00
abstract::The purpose of this article is to set out some important considerations on the main emerging antibiotic resistance patterns among anaerobic bacteria. The first point concerns the Bacteroides fragilis group and its resistance to the combination of β-lactam+β-lactamase inhibitor. When there is overproduction of cephalos...
journal_title:Anaerobe
pub_type: 杂志文章,评审
doi:10.1016/j.anaerobe.2010.10.002
更新日期:2010-12-01 00:00:00
abstract::This review focuses on the virulence arsenal of the most pathogenic species among Gram positive anaerobic cocci, Finegoldia magna according to recently published data from 2012 to 2016. Virulence factors like sortase dependent pili and F. magna adhesion factor (FAF) facilitate the start of the infection. Albumin bindi...
journal_title:Anaerobe
pub_type: 杂志文章,评审
doi:10.1016/j.anaerobe.2016.10.007
更新日期:2016-12-01 00:00:00
abstract::The mammalian gastrointestinal tract harbors a vast microbial ecosystem, known as the microbiota, which benefits host biology. Bacteroides fragilis is an important anaerobic gut commensal of humans that prevents and cures intestinal inflammation. We wished to elucidate aspects of gut colonization employed by B. fragil...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2011.05.017
更新日期:2011-08-01 00:00:00
abstract::To date nine species of anaerobic intestinal spirochaetes have been validly assigned to the genus Brachyspira. These include both pathogenic and non-pathogenic species. In the current study a genomic analysis of a novel spirochaete isolate was undertaken to determine whether it is a distinct species that previously ha...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2019.05.004
更新日期:2019-10-01 00:00:00
abstract::Treponema denticola is a spirochete that is involved in causing periodontal diseases. This bacterium can produce H2S from thiol compounds found in the gingival crevicular fluid. Determining how H2S is made by oral bacteria is important since this molecule is present at high levels in periodontally-diseased pockets and...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2020.102231
更新日期:2020-08-01 00:00:00
abstract::The minimum inhibitory concentration (MIC) of eight antimicrobial agents was determined by the agar dilution method for 80 isolates of Clostridium difficile from neonatal pigs with enteritis. MICs(50) for erythromycin, tilmicosin, and tylosin were relatively low (0.25-0.50 microg/mL), but MICs(90) (64 or > or =256 mic...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2004.01.003
更新日期:2004-02-01 00:00:00
abstract::The bacteria on the dorsum of the tongue are the most frequent cause of oral malodor; however, the bacterial flora of the tongue has not been well defined. Although recent studies have used DNA probes to detect the presence of certain periodontal pathogens, cultural studies have been limited because of the complexity ...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/S1075-9964(03)00109-4
更新日期:2003-10-01 00:00:00
abstract::Clostridium ventriculi (formerly Sarcina ventriculi) is a Gram-positive, obligate anaerobic coccus. Human infections due to this bacterium have rarely been reported, its involvement in the development of gastric ulcers and perforation has been suggested. We present a case of bacteremia due to C. ventriculi following a...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2019.05.005
更新日期:2019-10-01 00:00:00
abstract::Clostridium chauvoei is the etiologic agent of blackleg, a high mortality rate disease affecting mainly cattle and sheep. Carcasses of animals affected by the disease are the chief source of soil infection and considered as an ever-present threat to livestock health. A study was undertaken to examine the cross-contami...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2008.01.004
更新日期:2008-06-01 00:00:00
abstract::The first case of Clostridium difficile RT027 infection in the Czech Republic (CZ) was identified. The patient had been hospitalised in Germany prior to moving to CZ. Multiple-Locus Variable number tandem repeat Analysis revealed a genetic relatedness between the patient's isolate and RT027 isolate collected in the Ge...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2014.09.020
更新日期:2014-12-01 00:00:00
abstract::Although much newsprint is devoted to the subject of reducing the United States and other major developed countries dependence on their respective foreign energy sources; the most challenging issues for society is to provide long-term, sustainable energy sources to accommodate the global population as a whole. The pro...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2011.05.011
更新日期:2011-08-01 00:00:00
abstract::Obesity is among the most pressing health concerns in the world since it is increasingly common even in the developing world, and is clearly associated with increased risk for chronic debilitating diseases and death. Furthermore, obesity can influence the pathogenesis of infectious diseases by affecting the balance of...
journal_title:Anaerobe
pub_type: 杂志文章,评审
doi:10.1016/j.anaerobe.2014.12.009
更新日期:2015-08-01 00:00:00
abstract::The members of the phylum Fusobacteria and its two families, Fusobacteriaceae and Leptotrichiaceae, are distinguished at present mainly on the basis of their branching in the 16S rRNA gene trees and analysis of the internal transcribed spacer sequences in the 16S-23S rDNA. However, no biochemical or molecular characte...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2014.06.007
更新日期:2014-08-01 00:00:00
abstract::Analysis of restriction and modification activities in natural population of Selenomonas ruminantium have revealed the prevalence of CTGCAG (Pst I isoschizomers) recognizing restriction and/or modification systems in these bacteria. Pst I isoschizomeric restriction endonucleases were detected in 4 out of 15 strains te...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1006/anae.1998.0180
更新日期:1999-02-01 00:00:00
abstract::Community-associated Clostridium difficile infection (CA-CDI) represents 32% of all CDI cases based on U.S. population-based data. The current epidemic strain, NAP1, is the most prevalent strain causing these infections. Although complications, recurrence and death are uncommon, one-fourth of the CA-CDI patients are h...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2013.01.006
更新日期:2013-12-01 00:00:00
abstract::The human intestinal microbiota is necessary for the production of enterolignans from the dietary lignan secoisolariciresinol diglucoside (SDG). However, little is known about the bacteria that contribute to SDG conversion. Therefore, we aimed at describing the occurrence and activity of SDG metabolising bacteria. The...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2005.11.002
更新日期:2006-06-01 00:00:00
abstract::Evolution of antibiotic resistance in the anaerobes was reviewed using recent data covering 2000-2013 as compared to previous years. All studies reported growing moxifloxacin resistance in Bacteroides/Parabacteroides spp. in Europe and USA and in Clostridium difficile in Europe. In half or more studies, the resistance...
journal_title:Anaerobe
pub_type: 杂志文章,评审
doi:10.1016/j.anaerobe.2014.05.004
更新日期:2015-02-01 00:00:00
abstract::Propionic acid is commonly found as a fermentation product in the gastrointestinal tracts of food animals and has also been used to limit the microbial contaminants in animal feeds. Because propionic acid is known to have antibacterial activity, the propionic acid encountered by foodborne pathogens during their life c...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1006/anae.1998.0177
更新日期:1998-12-01 00:00:00
abstract::Since such things as sites of normal carriage, type and severity of infections produced, and antimicrobial susceptibility patterns may vary according to the particular species involved, accurate taxonomy for differentiation of species is important. For example, reclassification of the 'Bacteroides gracilis group' reve...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1006/anae.2002.0436
更新日期:2002-10-01 00:00:00
abstract::With the emergence of antibiotic resistance among Bacteroides fragilis group isolates the need of susceptibility testing in routine laboratories is increasing. The aims of the present study were to evaluate the disk diffusion method for susceptibility testing in case of different clinical isolates of Bacteroides spp b...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2014.10.008
更新日期:2015-02-01 00:00:00
abstract::Physiological changes in populations of Clostridium beijerinckii and Clostridium tetanomorphum were monitored by fluorescence staining and flow cytometry. To estimate the number of metabolically active cells in exponential growth, a combination of the dyes propidium iodide and carboxy fluorescein diacetate appeared to...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2013.10.006
更新日期:2014-10-01 00:00:00
abstract::Treponemes occur in the microflora of the dental plaque. Certain Treponema species that are frequently isolated from chronic periodontitis lesions are involved in its initiation and progression. In addition to mechanical instrumentation, antimicrobial agents are used as an adjunctive treatment modality for periodontit...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2017.10.005
更新日期:2017-12-01 00:00:00
abstract::Clostridioides difficile is the leading cause of healthcare-associated diarrhea. Although the incidence of C. difficile-associated diarrhea is increasing worldwide, bacteremia due to C. difficile is relatively rare and represents the least frequent extra-colonic manifestation of C. difficile infection. To date, only 6...
journal_title:Anaerobe
pub_type:
doi:10.1016/j.anaerobe.2020.102195
更新日期:2020-06-01 00:00:00
abstract::Anaerobes form a large part of microbial communities, and have begun to be specifically studied in both healthy and pathologic contexts. Porphyromonas is one of the top ten anaerobic taxa in the microbiome (anaerobiome) in healthy subjects. However, to date, most studies focused on the deleterious role of P. gingivali...
journal_title:Anaerobe
pub_type: 杂志文章,评审
doi:10.1016/j.anaerobe.2020.102230
更新日期:2020-06-30 00:00:00
abstract::There is concern that the bacterial colony counts present at the time of manufacture and listed on the probiotic package may not be reflective of the numbers viable colonies at the time of purchase and patient consumption thereby diminishing efficacy. We performed a colony count study of three separate samples of five...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2013.10.005
更新日期:2014-02-01 00:00:00
abstract::The objective of this work was to compare the biomass and community structure of bacteria, protozoa and archaea communities in samples of rumen and faeces of goats and to what extent the diet (alfalfa hay with or without supplemented oats) offered to them exert an influence. Four cannulated adult goats fistulated in t...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2013.11.012
更新日期:2014-10-01 00:00:00
abstract::Azo dyes are ubiquitous in products and often become environmental pollutants due to their anthropogenic nature. Azoreductases are enzymes which are present within many bacteria and are capable of breaking down the azo dyes via reduction of the azo bond. Often, though, carcinogenic aromatic amines are formed as metabo...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2013.07.005
更新日期:2013-10-01 00:00:00
abstract::Complex microbial communities are the functional core of anaerobic digestion processes taking place in biogas plants (BGP). So far, however, a comprehensive characterization of the microbiomes involved in methane formation is technically challenging. As an alternative, enriched communities from laboratory-scale experi...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2017.02.005
更新日期:2017-08-01 00:00:00