Abstract:
:Treponema denticola is a spirochete that is involved in causing periodontal diseases. This bacterium can produce H2S from thiol compounds found in the gingival crevicular fluid. Determining how H2S is made by oral bacteria is important since this molecule is present at high levels in periodontally-diseased pockets and the biological effects of H2S can explain some of the pathologies seen in periodontitis. Thus, it is of interest to identify the enzyme, or enzymes, involved in the synthesis of H2S by T. denticola. We, and others, have previously identified and characterized a T. denticola cystalysin, called HlyA, which hydrolyzes cysteine into H2S (and pyruvate and ammonia). However, there have been no studies to show that HlyA is, or is not, the only pathway that T. denticola can use to make H2S. To address this question, allelic replacement mutagenesis was used to make a deletion mutant (ΔhlyA) in the gene encoding HlyA. The mutant produces the same amount of H2S from cysteine as do wild type spirochetes, indicating that T. denticola has at least one other enzyme that can generate H2S from cysteine. To identify candidates for this other enzyme, a BLASTp search of T. denticola strain 33520 was done. There was one gene that encoded an HlyA homolog so we named it HlyB. Recombinant His-tagged HlyB was expressed in E. coli and partially purified. This enzyme was able to make H2S from cysteine in vitro. To test the role of HlyB in vivo, an HlyB deletion mutant (ΔhlyB) was constructed in T. denticola. This mutant still made normal levels of H2S from cysteine, but a strain mutated in both hly genes (ΔhlyA ΔhlyB) synthesizes significantly less H2S from cysteine. We conclude that the HlyA and HlyB enzymes perform redundant functions in vivo and are the major contributors to H2S production in T. denticola. However, at least one other enzyme can still convert cysteine to H2S in the ΔhlyA ΔhlyB mutant. An in silico analysis that identifies candidate genes for this other enzyme is presented.
journal_name
Anaerobejournal_title
Anaerobeauthors
Phillips L,Chu L,Kolodrubetz Ddoi
10.1016/j.anaerobe.2020.102231subject
Has Abstractpub_date
2020-08-01 00:00:00pages
102231eissn
1075-9964issn
1095-8274pii
S1075-9964(20)30087-1journal_volume
64pub_type
杂志文章相关文献
ANAEROBE文献大全abstract::The human intestinal microbiota is necessary for the production of enterolignans from the dietary lignan secoisolariciresinol diglucoside (SDG). However, little is known about the bacteria that contribute to SDG conversion. Therefore, we aimed at describing the occurrence and activity of SDG metabolising bacteria. The...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2005.11.002
更新日期:2006-06-01 00:00:00
abstract::Prokaryote diversity in the rumen of yak (Bos grunniens) and Jinnan cattle (Bos taurus) was estimated by 16S rDNA homology analysis. Two rumen 16S rDNA libraries were constructed. Of the 194 clones in the library of yak rumen, the sequences were mainly clustered to two phyla, low G+C Gram-positive bacteria (LGCGPB, 54...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2005.02.001
更新日期:2005-08-01 00:00:00
abstract::In order to study the microbial caecal ecosystem of wild and domestic rabbits through the fermentation characteristics and concentration and diversity of bacterial and archaeal communities, caecal samples from sixteen wild rabbits (WR) were contrasted with two groups (n = 4) of farm rabbits receiving low (LSF) or high...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2012.04.004
更新日期:2012-06-01 00:00:00
abstract::The toxic effects of heavy metals have been extensively documented in different organisms. Nevertheless, a lack of information exists with regard to this topic in the case of autochthonous microorganism communities. The aim of this study was to evaluate the toxic effects of zinc on the anaerobic microorganisms present...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2007.01.001
更新日期:2007-04-01 00:00:00
abstract::Physiological changes in populations of Clostridium beijerinckii and Clostridium tetanomorphum were monitored by fluorescence staining and flow cytometry. To estimate the number of metabolically active cells in exponential growth, a combination of the dyes propidium iodide and carboxy fluorescein diacetate appeared to...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2013.10.006
更新日期:2014-10-01 00:00:00
abstract::The comparison of the bacterial profile of intracellular (iDNA) and extracellular DNA (eDNA) isolated from cow rumen content stored under different conditions was conducted. The influence of rumen fluid treatment (cheesecloth squeezed, centrifuged, filtered), storage temperature (RT, -80 °C) and cryoprotectants (PBS-g...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2013.09.015
更新日期:2014-10-01 00:00:00
abstract::Prevotella (formerly Bacteroides) ruminicola is a numerous rumen bacterium which plays a significant role in the metabolism of proteins and peptides in the rumen. Measurement of the hydrolysis of synthetic aminopeptidase substrates by sonicated extracts and whole cells of different species of rumen bacteria indicated ...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1006/anae.1996.0065
更新日期:1997-02-01 00:00:00
abstract::Evolution of antibiotic resistance in the anaerobes was reviewed using recent data covering 2000-2013 as compared to previous years. All studies reported growing moxifloxacin resistance in Bacteroides/Parabacteroides spp. in Europe and USA and in Clostridium difficile in Europe. In half or more studies, the resistance...
journal_title:Anaerobe
pub_type: 杂志文章,评审
doi:10.1016/j.anaerobe.2014.05.004
更新日期:2015-02-01 00:00:00
abstract::We investigated the change in chlortetracycline resistance in 360 Escherichia coli strains separated from the feces of piglets fed fermented liquid feed (FLF) in comparison with those fed dry feed (control). The total amount of lactic acid bacteria in feces was 8.77 x 10(8) CFU/g DM at weaning, which increased to 1.23...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2008.07.001
更新日期:2008-10-01 00:00:00
abstract::Several studies have suggested the association of disturbed genital tract microbiota with infertility. Our aim was to clarify the influence of sexual intercourse on partner's genital tract microbiota in infertile couples. Seventeen couples were studied, and in 5 men inflammatory prostatitis (IP) was diagnosed. Semen s...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2011.04.015
更新日期:2011-12-01 00:00:00
abstract::Ice used for direct human consumption or to preserve foods and cool down drinks can be contaminated with pathogenic microorganisms and may potentially become a vehicle for consumer's infection. To evaluate physical, chemical and microbiological quality of commercial ice and ice used for fish and seafood, 100 ice sampl...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2011.06.005
更新日期:2011-12-01 00:00:00
abstract::Sporulation during Clostridioides difficile infection (CDI) contributes to recurrent disease. Cell division and sporulation both require peptidoglycan biosynthesis. We show C. difficile growth and sporulation is attenuated by antisenses to murA and murC or the MurA inhibitor fosfomycin. Thus, targeting the early steps...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2019.102129
更新日期:2020-02-01 00:00:00
abstract::Prebiotics and probiotics are increasingly being used to produce potentially synbiotic foods, particularly through dairy products as vehicles. It is well known that both ingredients may offer benefits to improve the host health. This research aimed to evaluate the prebiotic potential of novel petit-suisse cheeses usin...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2007.05.003
更新日期:2007-10-01 00:00:00
abstract::Lacto-N-biose I (LNB) is a potential factor for the selective growth of bifidobacteria. We previously reported that the species of bifidobacteria predominant in infant intestines might use LNB. We aimed to assess the prebiotic properties of LNB in comparison to other oligosaccharides using an in vitro fermentation sys...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2012.12.007
更新日期:2013-02-01 00:00:00
abstract::Despite extensive research on the epidemiology of pathogenic clostridia in dogs and cats, most published studies focus on a selected animal population and/or a single veterinary medical centre. We assessed the burden of Clostridium perfringens and C. difficile shedding by small animals in 17 veterinary clinics located...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2017.06.023
更新日期:2017-12-01 00:00:00
abstract::This review summarizes the information that supports the potential importance of anaerobic bacteria in the pharyngo-tonsillitis (PT) associated with infectious mononucleosis (IM). Evidence supportive of the potential of involvement of bacteria in IM was provided by several studies that showed increased recovery of bac...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2005.04.004
更新日期:2005-12-01 00:00:00
abstract::The objective of this study was to develop the strain-specific PCR primers for Fusobacterium nucleatum subsp. fusiforme ATCC 51190(T) and F. nucleatum subsp. vincentii ATCC 49256(T) based on the nucleotide sequence of the Fs17 and Fv35 DNA probes, respectively. The strain specificity was tested against 10 type strains...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2009.04.003
更新日期:2010-02-01 00:00:00
abstract::The purpose of this article is to set out some important considerations on the main emerging antibiotic resistance patterns among anaerobic bacteria. The first point concerns the Bacteroides fragilis group and its resistance to the combination of β-lactam+β-lactamase inhibitor. When there is overproduction of cephalos...
journal_title:Anaerobe
pub_type: 杂志文章,评审
doi:10.1016/j.anaerobe.2010.10.002
更新日期:2010-12-01 00:00:00
abstract:BACKGROUND CONTEXT:Delayed-onset surgical site infections following spinal instrumentation are uncommon and often present with chronic pain and implant failure. Anaerobic organisms are rarely implicated and identified with difficulty in these infections. PURPOSE:We report a case of vertebral osteomyelitis and epidural...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2015.08.004
更新日期:2015-10-01 00:00:00
abstract::Eight spindle-shaped bacteria were isolated from clinical samples in Japan and investigated for their taxonomic position. Phylogenetic trees (based on 16S rRNA, rpoB, zinc protease, and gyrB gene sequence comparisons) showed distinct clustering of eight strains with the type strain of Fusobacterium nucleatum and its c...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2021.102323
更新日期:2021-01-27 00:00:00
abstract::Here, we report a case of fatal sepsis resulting from an intra-abdominal infection caused by a Bacteroides fragilis strain containing a CfiA4 metallo-β-lactamase and an upstream insertion sequence (IS) element. Meropenem was used as empiric therapy for septic shock as a result of the intra-abdominal infection, althoug...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2017.01.010
更新日期:2017-04-01 00:00:00
abstract::Analysis of restriction and modification activities in natural population of Selenomonas ruminantium have revealed the prevalence of CTGCAG (Pst I isoschizomers) recognizing restriction and/or modification systems in these bacteria. Pst I isoschizomeric restriction endonucleases were detected in 4 out of 15 strains te...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1006/anae.1998.0180
更新日期:1999-02-01 00:00:00
abstract::Clostridium difficile is a spore-forming anaerobic intestinal pathogen that causes Clostridium difficile infection (CDI). C. difficile is the leading cause of toxin-mediated nosocomial antibiotic-associated diarrhea. The pathogenesis of CDI is attributed to two major virulence factors, TcdA and TcdB toxins, that cause...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2017.04.001
更新日期:2017-06-01 00:00:00
abstract::Due to increasing antibiotic resistance among anaerobic bacteria, routine antimicrobial susceptibility testing is recommended by the Clinical and Laboratory Standards Institute (CLSI). This study compared the minimum inhibitory concentrations (MICs) from 920 Clostridium difficile isolates tested against seven antimicr...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2017.02.006
更新日期:2017-04-01 00:00:00
abstract::Gut fungal-specific PCR primers have been used to selectively amplify the ITS1 region of gut fungal rDNA recovered from faeces of domestic and wild animals to investigate population diversity. Two different gel-based methods are described for separating populations of gut fungal rDNA amplicons, namely (1) denaturing g...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2009.05.003
更新日期:2010-04-01 00:00:00
abstract::A longitudinal study of Clostridium difficile colonization in piglets was performed on a conventional swine farm in Ontario, Canada. Fecal samples were collected from 10 sows prior to their expected farrowing date, and then from all their piglets on days 2, 7, 30, 44 and 62 of life. C. difficile was isolated from 4/10...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2010.08.001
更新日期:2010-10-01 00:00:00
abstract::The bacteriolytic activity of nutritionally variant streptococci (NVS), fastidious microaerophilic bacteria, which are members of the genera Abiotrophia and Granulicatella, was characterized in a renaturating SDS polyacrylamide gel electrophoresis system. Bacteriolytic profiles appeared quite different for the three s...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2007.08.002
更新日期:2007-10-01 00:00:00
abstract::We present the first case report of osteomyelitis due to Clostridium hydrogeniformans in a previously healthy 18-year-old male. He was admitted to our hospital because of an open contaminated fracture of the right arm after being blown into a drain in a motorbike accident. He underwent surgical debridement and treatme...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2017.02.013
更新日期:2017-06-01 00:00:00
abstract::In this paper, the bioactivity of cell-free extracts obtained by dairy propionibacteria strains was investigated. Probiotic bifidobacteria and lactobacilli were used as microbial targets. The extracellular filtrates were added as ingredient (1% v/v) into the growth medium; the effect of cell-free filtrates was evaluat...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2014.09.016
更新日期:2014-12-01 00:00:00
abstract::Susceptibility of intestinal bacteria to various antimicrobial agents in vitro, together with levels of those agents achieved in the gut, provides information on the likely impact of the agents on the intestinal flora. Orally administered drugs that are poorly absorbed may be useful for treatment of intestinal infecti...
journal_title:Anaerobe
pub_type: 杂志文章
doi:10.1016/j.anaerobe.2004.04.003
更新日期:2004-08-01 00:00:00