Abstract：
:Clostridium difficile is a spore-forming anaerobic intestinal pathogen that causes Clostridium difficile infection (CDI). C. difficile is the leading cause of toxin-mediated nosocomial antibiotic-associated diarrhea. The pathogenesis of CDI is attributed to two major virulence factors, TcdA and TcdB toxins, that cause the symptomatic infection. C. difficile also expresses a number of key proteins, including cell wall proteins (CWPs). S-layer proteins (SLPs) are CWPs that form a paracrystalline surface array that coats the surface of the bacterium. SLPs have a role in C. difficile binding to the gastrointestinal tract, but their importance in virulence need to be better elucidated. Here, we describe bottom-up proteomics analysis of surface-enriched proteins fractions obtained through glycine extraction of five C. difficile clinical isolates from Brazil using gel-based and gel-free approaches. We were able to identify approximately 250 proteins for each strain, among them SlpA, Cwp2, Cwp6, CwpV and Cwp84. Identified CWPs presented different amino acid coverage, which might suggest differences in post-translational modifications. Proteomic analysis of SLPs from ribotype 133, agent of C. difficile outbreaks in Brazil, revealed unique proteins and provided additional information towards in depth characterization of the strains causing CDI in Brazil.
journal_name
Anaerobejournal_title
Anaerobeauthors
Ferreira TG,Moura H,Barr JR,Pilotto Domingues RMC,Ferreira EOdoi
10.1016/j.anaerobe.2017.04.001subject
Has Abstractpub_date
2017-06-01 00:00:00pages
120-128eissn
1075-9964issn
1095-8274pii
S1075-9964(17)30068-9journal_volume
45pub_type
杂志文章相关文献
ANAEROBE文献大全abstract：:Prevotella dentalis is a Gram-negative anaerobic rod involved in various human diseases, especially oral infections. We report a rare case of a pleural effusion due to this microorganism in an elderly patient. An 88-year-old man with chronic respiratory disease presented with a left pleural effusion for more than 1 mo...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2018.09.004
更新日期：2018-12-01 00:00:00
abstract：:Bifidobacteria are part of the human gastrointestinal microbiota and are used as probiotics in functional food products because of their health promoting properties. However, only few data are available on the phenotypic characteristics displayed by human bifidobacteria strain populations. In this study we compared th...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2013.04.005
更新日期：2013-06-01 00:00:00
abstract：:As shown previously, a recombinant alphatoxin variant (rAT121A/91) constructed from the naturally occurring Clostridium perfringens mutant strain 121A/91, was devoid of enzymatic (PLC), hemolytic and lethal activity (18). In the present study, the recombinant variant was altered by an oligonucleotide-directed reversio...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2005.06.003
更新日期：2006-02-01 00:00:00
abstract：:Recently new resin-based BacT/Alert FAN Plus bottles containing antibiotic-binding polymeric beads, were introduced as an improvement of the charcoal-based FAN bottles for the recovery of bacteria and fungi. To assess the performance of the novel anaerobic FN plus bottles in the detection of anaerobic organisms, we co...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2015.07.010
更新日期：2015-10-01 00:00:00
abstract：:Diterpenes are an important class of plant metabolites that can be used in the search for new antibacterial agents. ent-Copalic acid (CA), the major diterpene in Copaifera species exudates, displays several pharmacological properties. This study evaluates the CA antibacterial potential against the anaerobic bacteria P...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2018.05.013
更新日期：2018-08-01 00:00:00
abstract：:Staphylococcus aureus is an opportunistic pathogen that can colonize human and animal intestinal tracts, causing certain gastrointestinal diseases. The adherence of enteric pathogens to host intestinal epithelial cells is important for their pathogenesis. In the present study, Lactobacillus salivarius and Lactobacillu...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2012.08.001
更新日期：2012-10-01 00:00:00
abstract：:Aspiration pneumonia is an infectious disease of the lungs caused by inhalation of saliva or foods, associated with swallowing dysfunction. Therefore, the major causative organisms are oral or gastric bacteria. In this study, we evaluated the antimicrobial susceptibility patterns of the anaerobic bacteria which can ca...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2019.04.002
更新日期：2019-06-01 00:00:00
abstract：:The formation of dental plaque biofilm by specific Gram-negative rods and spirochetes plays an important role in the development of periodontal disease. The aim of this study was to characterize biofilm formation by Fusobacterium nucleatum and Capnocytophaga ochracea. Coaggregation between F. nucleatum and Capnocytoph...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2012.01.001
更新日期：2012-02-01 00:00:00
abstract：:We investigated the change in chlortetracycline resistance in 360 Escherichia coli strains separated from the feces of piglets fed fermented liquid feed (FLF) in comparison with those fed dry feed (control). The total amount of lactic acid bacteria in feces was 8.77 x 10(8) CFU/g DM at weaning, which increased to 1.23...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2008.07.001
更新日期：2008-10-01 00:00:00
abstract：:Treponemes occur in the microflora of the dental plaque. Certain Treponema species that are frequently isolated from chronic periodontitis lesions are involved in its initiation and progression. In addition to mechanical instrumentation, antimicrobial agents are used as an adjunctive treatment modality for periodontit...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2017.10.005
更新日期：2017-12-01 00:00:00
abstract：:Nitrite reductase (cytochrome cd1) was purified to electrophoretic homogeneity from the soluble extract of the marine denitrifying bacterium Pseudomonas nautica strain 617. Cells were anaerobically grown with 10 mM nitrate as final electron acceptor. The soluble fraction was purified by four successive chromatographic...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1006/anae.1995.1021
更新日期：1995-08-01 00:00:00
abstract：:In the past few years, many studies revealed a remarkable genetic variability in Bacteroides fragilis species, and the existence of two divisions was proposed according to presence or absence of the cfiA (metallo-β-lactamase/carbapenemase) gene. The aim of this study was to evaluate the use of DNA sequence analysis fo...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2012.11.005
更新日期：2013-02-01 00:00:00
abstract：:Bacterial strain QZ1 was isolated from sludge of anoxic sulfide-oxidizing (ASO) reactor. Based on 16S rDNA sequence analysis and morphological characteristics, the isolate was identified as Pseudomonas stutzeri. The isolate was found to be a facultative chemolithotroph, using sulfide as electron donor and nitrite as e...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2009.03.009
更新日期：2009-08-01 00:00:00
abstract：:Sixty-six nonduplicate Bacteroides clinical isolates collected at Marmara University Hospital were tested to investigate carbapenem and metronidazole resistance profiles and to detect the resistance genes (cfiA and nim) and related insertion sequence (IS) elements. The study found that there were no strains resistant ...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2011.10.004
更新日期：2012-02-01 00:00:00
abstract：:Clostridium difficile causes diarrhoeal diseases ranging from asymptomatic carriage to a fulminant, relapsing, and potentially fatal colitis. Endospore production plays a vital role in transmission of infection, and in order to cause disease these spores must then germinate and return to vegetative cell growth. Type B...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2010.10.001
更新日期：2010-12-01 00:00:00
abstract：:The extracellular matrix protein fibronectin (Fn) is known to bind to the surface of Clostridium perfringens cells. Fn is a disulfide-linked homodimer protein, with each Fn polypeptide consisting of three types of repeating modules: 12 type I, 2 type II, and 15-17 type III modules. To determine the epitope on Fn recog...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2014.11.007
更新日期：2015-08-01 00:00:00
abstract：:Several factors affect the composition of species that inhabit our intestinal tract, including mode of delivery, genetics and nutrition. Antimicrobial peptides and proteins secreted in the gastrointestinal tract are powerful tools against bacteria. Lactoferrin (LF) inhibits the growth of several bacterial species, suc...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2020.102232
更新日期：2020-08-01 00:00:00
abstract：:Susceptibility of intestinal bacteria to various antimicrobial agents in vitro, together with levels of those agents achieved in the gut, provides information on the likely impact of the agents on the intestinal flora. Orally administered drugs that are poorly absorbed may be useful for treatment of intestinal infecti...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2004.04.003
更新日期：2004-08-01 00:00:00
abstract：:Linezolid is a novel oxazolidinone antibacterial agent active against staphylococci (including methicillin-resistant strains), enterococci (including vancomycin-resistant strains), streptococci (including penicillin-intermediate and -resistant Streptococcus pneumoniae), and other aerobic and facultative bacteria. The ...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/S1075-9964(03)00004-0
更新日期：2003-02-01 00:00:00
abstract：:Ice used for direct human consumption or to preserve foods and cool down drinks can be contaminated with pathogenic microorganisms and may potentially become a vehicle for consumer's infection. To evaluate physical, chemical and microbiological quality of commercial ice and ice used for fish and seafood, 100 ice sampl...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2011.06.005
更新日期：2011-12-01 00:00:00
abstract：:Gut fungal-specific PCR primers have been used to selectively amplify the ITS1 region of gut fungal rDNA recovered from faeces of domestic and wild animals to investigate population diversity. Two different gel-based methods are described for separating populations of gut fungal rDNA amplicons, namely (1) denaturing g...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2009.05.003
更新日期：2010-04-01 00:00:00
abstract：:Bacteroides thetaiotaomicron maybe one of the most adaptable intestinal bacteria due to its complex genome. Known to be an opportunistic pathogenic anaerobe, B. thetaiotaomicron has recently been described as a symbiont with anti-inflammatory properties. In this study, peptide mass finger printing technique was used t...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2014.04.008
更新日期：2014-08-01 00:00:00
abstract：:This review focuses on the virulence arsenal of the most pathogenic species among Gram positive anaerobic cocci, Finegoldia magna according to recently published data from 2012 to 2016. Virulence factors like sortase dependent pili and F. magna adhesion factor (FAF) facilitate the start of the infection. Albumin bindi...
journal_title：Anaerobe
pub_type： 杂志文章,评审
doi：10.1016/j.anaerobe.2016.10.007
更新日期：2016-12-01 00:00:00
abstract：:Three cases of Clostridium aldenense bacteremia are reported. C. aldenense is also associated with intra-abdominal infections and closely resembles Clostridium clostridioforme and therefore may be misidentified. C. aldenense may be a more frequent pathogen than appreciated and is generally fluoroquinolone resistant. ...
journal_title：Anaerobe
pub_type： 杂志文章,评审
doi：10.1016/j.anaerobe.2010.08.004
更新日期：2010-10-01 00:00:00
abstract：:This study aimed to determine the probiotic potential of 100 strains of Lactic Acid Bacteria (LAB) isolated from different intestinal segments of indigenous poultry in Tunisia. The strains were submitted to a battery of standard tests and criteria commonly used for determining their probiotic properties and attributes...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2012.05.001
更新日期：2012-08-01 00:00:00
abstract：:Fusobacterium nucleatum is a Gram-negative anaerobe, found in a number of different areas of human and animal bodies as part of the resident microbiota. However, it also appears to be involved in polymicrobial infections in such sites. It occurs in the oral cavity where it is a prominent member of various bacterial co...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1006/anae.1997.0140
更新日期：1998-04-01 00:00:00
abstract：:Clostridium chauvoei is the etiologic agent of blackleg in cattle, inducing fever, severe myonecrosis, oedemic lesions and ultimately death of infected animals. The pathogen often results in such rapid death that antibiotic therapy is futile and thus vaccination is the only efficient strategy in order to control the d...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2019.02.011
更新日期：2019-04-01 00:00:00
abstract：:We studied the serum bactericidal activity (SBA) of moxifloxacin and levofloxacin against common pathogens associated with complicated intra-abdominal infections. Ten healthy volunteers received a single dose of moxifloxacin (400 mg) and levofloxacin (750 mg) and serum samples were collected at 2, 4, 8, 12, and 24h af...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2007.09.005
更新日期：2008-02-01 00:00:00
abstract：:Clostridia represent a diverse group of spore-forming gram positive anaerobes that include several pathogenic species. In general, diseases caused by clostridia are a result of intoxication of the infected host. Thus, clostridial toxins have been targeted for diagnostic, therapeutic, and preventive strategies against ...
journal_title：Anaerobe
pub_type： 杂志文章,评审
doi：10.1016/j.anaerobe.2013.08.009
更新日期：2013-12-01 00:00:00
abstract：:Highest antimicrobial activity of peptide ST4SA (51,200 AU/mL) was recorded after 14 h of growth in MRS broth with optimal production at pH 6.0 or 6.5. Growth of strain ST4SA in the presence of tryptone, yeast extract, or a combination of the two, yielded 102,400 AU/mL. An increase in production of peptide ST4SA to 10...
journal_title：Anaerobe
pub_type： 杂志文章
doi：10.1016/j.anaerobe.2008.11.002
更新日期：2009-06-01 00:00:00
